A field experiment was carried out at Agricultural Research Station, Faculty of Agriculture (Suluq), Benghazy University in 2023/ 2024 winter season to study four nitrogen fertilizer levels (50, 100, 150 and 200 kg/ ha) on growth and yield of three bread wheat varieties, i.e. Alfi, Kasy and Behooth 210. Split-plot design in three replicated was used, where nitrogen levels was randomly distributed on whole plot and varieties were allocated in sub-plot. Increasing nitrogen levels from 50 to 200 kg/ ha significantly and gradually increased all studied traits, except number of sterile plants. Behooth 210 variety had the tallest plants and spikes (111.25 and 14.58 cm), respectively, highest number of grains/ spike (79.50) and heaviest 100-grain weight (5.10 g). However, Alfi variety the highest number of sterile plants (42.41), highest grain yield and harvest index (4.90 ton/ ha and 21.87 %), respectively. Alfi variety fertilized with 200 kg N/ ha produced the highest grain yield (5.70 t/ ha), while Behooth variety fertilized with (200 kg N/ ha) produced the highest number of grains/ spike (84.93), heaviest 100-grain weight (5.87 g) and highest biological yield (26.37 t/ ha). Keywords: Agricultural Research Station, wheat, nitrogen levels, grain yield, harvest index, Alfi, Kasy, Behooth.
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