Talent Program Research Articles

Multiportfolio Optimization: A Fairness-Aware Target-Oriented Model

Problem definition: We consider a multiportfolio optimization problem in which nonlinear market impact costs result in a strong dependency of one account’s performance on the trading activities of the other accounts. Methodology/results: We develop a novel target-oriented model that jointly optimizes the rebalancing trades and the split of market impact costs. The key advantages of our proposed model include the consideration of clients’ targets on investment returns and the incorporation of distributional uncertainty. The former helps fund managers to circumvent the difficulty in identifying clients’ utility functions or risk parameters, whereas the latter addresses a practical challenge that the probability distribution of risky asset returns cannot be fully observed. Specifically, to evaluate the quality of multiple portfolios’ investment payoffs in achieving targets, we propose a new class of performance measures, called fairness-aware multiparticipant satisficing (FMS) criteria. These criteria can be extended to encompass distributional uncertainty and have the salient feature of addressing the fairness issue with the collective satisficing level as determined by the least satisfied participant. We find that, structurally, the FMS criteria have a dual connection with a set of risk measures. For multiportfolio optimization, we consider the FMS criterion with conditional value-at-risk being the underlying risk measure to further account for the magnitude of shortfalls against targets. The resulting problem, although nonconvex, can be solved efficiently by solving an equivalent converging sequence of tractable subproblems. Managerial implications: For the multiportfolio optimization problem, the numerical study shows that our approach outperforms utility-based models in achieving targets and in out-of-sample performance. More generally, the proposed FMS criteria provide a new decision framework for operational problems in which the decision makers are target-oriented rather than being utility maximizers and issues of fairness and ambiguity should be considered. Funding: This work was supported by the Hong Kong Research Grants Council [Grants 14210821, 16204521], Leading Talent Program of Guangdong Province [Grant 2016LJ06D703], and the National Natural Science Foundation of China [Grants 71971187, 72171156, 72231002, 72331009]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2021.0363 .

Ovarian ferroptosis induced by androgen is involved in pathogenesis of PCOS.

Does ovarian ferroptosis play an active role in the development of polycystic ovary syndrome (PCOS)? Increased ovarian ferroptosis was present in PCOS ovaries and the inhibition of ferroptosis with ferrostatin-1 (Fer-1) ameliorated polycystic ovary morphology and anovulation. Programmed cell death plays a fundamental role in ovarian follicle development. However, the types and mechanisms of cell death involved in the ovary are yet to be elucidated. Ferroptosis is a recently discovered iron-dependent programmed cell death. Impaired iron metabolism and cell death have been observed in women with PCOS, the main cause of anovulatory infertility. Additionally, previous studies reported that an abnormal expression of noncoding RNA may promote ferroptosis in immortalized ovarian granulosa cell lines. However, little is known about whether ovarian ferroptosis is increased in PCOS, and there is insufficient direct evidence for a role of ferroptosis in PCOS, and the underlying mechanism. Moreover, the effect of the inhibition of ferroptosis with Fer-1 in PCOS remains unclear. Ferroptosis was evaluated in human granulosa cells (hGCs) from non-PCOS (n = 6-16) and PCOS (n = 7-18) patients. The experimental study was completed in vitro using primary hGCs from women undergoing IVF. Improvements in PCOS indicators following ferroptosis inhibition with Fer-1 were investigated in a dehydroepiandrosterone (DHEA)-induced PCOS rat model (n = 8 per group). Ovarian ferroptosis was evaluated in the following ways: by detecting iron concentrations via ELISA and fluorescent probes; measuring malondialdehyde (MDA) concentrations via ELISA; assessing ferroptosis-related protein abundance with western blotting; observing mitochondrial morphology with transmission electron microscopy; and determining cell viability. Primary hGCs were collected from women undergoing IVF. They were treated with dihydrotestosterone (DHT) for 24 h. The effect of DHT on ferroptosis was examined in the presence or absence of small interfering RNA-mediated knockdown of the putative receptor coregulator for signaling molecules. The role of ovarian ferroptosis in PCOS progression was explored in vivo in rats. The DHEA-induced PCOS rat model was treated with the ferroptosis inhibitor, Fer-1, and the oocytes and metaphase II oocytes were counted after ovarian stimulation. Additionally, rats were treated with the ferroptosis inducer, RSL3, to further explore the effect of ferroptosis. The concentrations of testosterone, FSH, and LH were assessed. Increased ferroptosis was detected in the ovaries of patients with PCOS and in rats with DHEA-induced PCOS. Increased concentrations of Fe2+ (P < 0.05) and MDA (P < 0.05), and upregulated nuclear receptor coactivator 4 protein levels, and downregulated ferritin heavy chain 1 (FTH1) and glutathione peroxidase 4 (GPX4) proteins were observed in the hGCs in patients with PCOS and ovaries of PCOS rats (P < 0.05 versus control). DHT was shown to induce ferroptosis via activation of NOCA4-dependent ferritinophagy. The inhibition of ferroptosis with Fer-1 in rats ameliorated a cluster of PCOS traits including impaired glucose tolerance, irregular estrous cycles, reproductive hormone dysfunction, hyperandrogenism, polycystic ovaries, anovulation, and oocyte quality (P < 0.05). Treating rats with RSL3 resulted in polycystic ovaries and hyperandrogenism (P < 0.05). N/A. Although ovarian-targeted ferroptosis inhibition may be a more targeted treatment for PCOS, the underlying mechanisms in the cycle between ferroptosis and hyperandrogenism require further exploration. Additionally, since PCOS shows high heterogeneity, it is important to investigate whether ferroptosis increases are present in all patients with PCOS. Androgen-induced ovarian ferroptosis appears to play a role in the pathogenesis of PCOS, which potentially makes it a promising treatment target in PCOS. This study was supported by the National Key R&D Program of China (2023YFC2705500, 2023YFC2705505, 2019YFA0802604), National Natural Science Foundation of China (No. 82130046, 82320108009, 82101708, 82101747, and 82001517), Shanghai leading talent program, Innovative research team of high-level local universities in Shanghai (No. SHSMU-ZLCX20210201, No. SSMU-ZLCX20180401), Shanghai Jiaotong University School of Medicine, Affiliated Renji Hospital Clinical Research Innovation Cultivation Fund Program (RJPY-DZX-003) and Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (No. 20161413), Shanghai's Top Priority Research Center Construction Project (2023ZZ02002), and Three-Year Action Plan for Strengthening the Construction of the Public Health System in Shanghai (GWVI-11.1-36). The authors report no competing interests.

Cluster Preface: Biomimetic Synthesis

Jun Deng (left) received his B.Sc. from Lanzhou University and his Ph.D. from the Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences (2014) with Professor Ang Li. After a short period of postdoctoral research in the lab of Professor Andrew Myers at Harvard University (2014–2016), he was appointed a Pioneer ‘Hundred Talents Program’ Researcher at Kunming Institute of Botany, Chinese Academy of Sciences in 2017. In December 2020, he moved to Nankai University where he is a professor at the College of Chemistry. He received the Thieme Journals Award (2020). His research focuses on natural product total synthesis and medicinal chemistry. Yefeng Tang (right) earned his B.S. from Lanzhou University (1995–1999) and his M.S. from the Institute of Materia Medica, Chinese Academy of Medical Science (1999–2002). From 2003 to 2006, he pursued his Ph.D. under the guidance of Professor Zhen Yang and Professor Jiahua Chen at Peking University. Subsequently, he conducted postdoctoral research with Prof. K. C. Nicolaou at The Scripps Research Institute between 2006 and 2009. In 2010, he commenced his independent career at the School of Pharmaceutical Sciences, Tsinghua University, where he is now a tenured professor. His primary research interests encompass the total synthesis of natural products, the development of synthetic methodologies, and the discovery of antiviral and anti-aging drugs.

Gender differences in Dutch research funding over time: A statistical investigation of the innovation scheme 2012-2021.

In 2015, the Dutch research council, NWO, took measures to combat gender bias disadvantaging female applicants in a popular three-tiered funding scheme called the Talent Programme. The innovation scheme consists of three grants for different career stages, called Veni, Vidi and Vici. This paper studies the question whether or not NWO has been successful in removing gender differences in their funding procedure. Using all available data from 2012 onwards of grant applications in the Talent Programme (16,249 applications of which 2,449 received funding), we study whether these measures had an effect using binomial generalized linear models. We find strong statistical evidence of a shift in gender effects in favour of female applicants in the first tier, the Veni (p < .001). Significant gender differences are not found in the two other tiers, the Vidi and Vici schemes. In recent years, female applicants are more likely to be awarded with a Veni grant than male applicants and this gender gap has increased over time. This suggests that gender differences still exist in the assessment of Talent Programme submissions, albeit in a different direction than a decade ago.

Project management as a component of talent management programmes

The aim of the article is to explore the poorly researched phenomenon of the use of projects as a talent development tool within talent management programmes. The author conducted a literature scoping review to examine the state of knowledge on the use of projects and project management in talent management programmes. The author identified 7 articles that address the research question. The literature review confirmed that the phenomenon exists, but it is poorly researched. Factors influencing the effectiveness and efficiency of using projects in talent management were identified, conclusions were drawn regarding the potential impact of talent management on projects carried out within talent management programmes and a preliminary conceptualization of the relationships between projects and talent management programmes was presented. This study may serve as a basis for planning field studies exploring talent management programmes that use projects as one of the tools for employee development.

Throwing it out there: Grip on multidimensional performance characteristics of judoka – a systematic review

Judo is a dynamic and complex sport, and therefore talent programs may benefit from a multidimensional approach to identify clear and reliable performance characteristics in order to monitor and train athletes. However, from both a practical and theoretical point of view, understanding on this topic is limited. This systematic review therefore aimed to (1) identify which multidimensional performance characteristics can discriminate between different performance levels of judoka and (2) find the gaps in literature. To categorize the findings the Groningen Sport Talent Model (GSTM) was applied. A systematic search of MEDLINE, PsycINFO, PubMed, and Web of Science was performed following the PRISMA guidelines. In total 23 studies with good to excellent methodological quality were included. All performance characteristics of the GSTM (anthropometrical, physiological, technical, tactical, and psychological) were represented in the included studies. There is strong evidence to suggest that a broad set of physiological characteristics is needed to manage the demands of judo combats. Additionally, there is strong evidence that performance characteristics related to grip fighting discriminate between judoka of different performance levels and higher performance level judoka are characterized by the ability to throw their opponent and to variate in technique. The findings of this review can be used as a basis for talent development and identification in judo, and to optimize training programs. Future research should focus on increasing the understanding of psychological characteristics of judoka and the multidimensional talent trajectory by conducting longitudinal studies.

Do the Total Reward and Talent Management Program Affect the Retention and Performance of Generation Y Employees?

Currently, the Indonesian national workforce is dominated by generation Y. Generation Y is the generation born between 1980-2000. Based on literature studies, this generation is known as the generation that easily leaves the workplace if they see career opportunities. Employees who move easily will affect company performance. With changes in employee demographics, it is important to examine strategies and factors that influence employee retention. This article tries to discuss and explore the factors that influence the retention and performance of generation Y employees through total rewards and talent management programs. This research uses a questionnaire survey conducted on employees of Bank Syariah Indonesia in Lampung Province, Indonesia. A total of 200 generation Y employee respondents in Lampung participated in this research. The analysis model in data processing uses SEM Lisrel 8.8. The findings reveal that total rewards are proven to influence the retention and performance of generation Y employees. The talent management program has no direct influence on the performance of generation Y employees. Intrinsic rewards are proven to have more influence on the retention and performance of generation Y employees. Talent management programs influence the performance of generation Y employees if mediated by retention.

EVS-BASED THERAPIES FOR INTERVERTEBRAL DISC REGENERATION: WHERE DO WE STAND?

Within the field of disc degeneration-related low back pain, the spine community has been increasingly acknowledging the regenerative potential of extracellular vesicles (EVs). EVs are small lipid bilayer-delimited particles naturally released by cells, involved in intercellular signaling. They do so by interacting with recipient cells and releasing their biological cargo (e.g., mRNA, miRNA, DNA, protein, lipid)EVs derived from mesenchymal stromal cells and, more recently, also EVs from notochordal cells, the cells residing within the core of the juvenile human disc, are being actively studied. In general, they have been proposed to mitigate inflammation/catabolic processes, reduce apoptosis, stimulate proliferation and even improve the matrix producing capacity of the treated cells. Within this context, appropriate characterization of EVs is essential to increase the level of evidence that the reported effects are indeed EV-associated. To analyze the purity and biochemical composition of EV preparations the International Society for Extracellular Vesicles (ISEV) has prepared guidelines recommending the analysis of multiple (EV) markers, as well as proteins co-isolated/recovered with EVs. Alongside, to prove that the effects are EV-associated and not due to co-isolated factors from the tissue or cells used to derive the EVs, appropriate technical controls need to be taken along (during cell/tissue culture). As such the question arises: “what is the evidence so far?”While from a fundamental perspective EVs are very appealing, the use of natural EVs in clinical applications is challenging. It comes with drawbacks, including biologic variability, yield, cumbersome isolation, and challenging upscaling and storage to achieve industrial levels. To date there is no FDA-approved EV-based therapy for disc-related lower back pain.Nonetheless, EV-based therapeutic approaches have unique advantages over the use of (pluripotent) stem cell-based therapies, such as a high biologic, but low immunogenic and tumorigenic potential.Acknowledgements: This talk is based on experiences from part of the project NC-CHOICE [no. 19251] of the research talent programme VICI financed by the Dutch Research Council (NWO) and the iPSpine project that receives funding from the European Union's Horizon 2020 research and innovation program under grant agreement no. 825925.

Exploring the impact of an English talent program on college students' language proficiency: A mixed-methods study

This study examined the impact of an English talent (ET) program on the English language growth of college students in China. Using mixed methods, the researchers found that the ET program had a statistically significant impact on participants’ English proficiency scores across all subtests (i.e., listening, reading, and writing/translation) compared to those of a group of students enrolled in traditional English courses. ET teachers’ reflections indicated that the program improved their students’ listening, speaking, reading, and writing skills and prepared them to compete in national English competitions. The study highlights the effectiveness of ET programs in English language education at the college level.

Analysing the Influence of Talent Management Strategies on Organisational Performance at Namibia Power Corporation

Talent management strategies have a huge impact on the productivity of employees in various organisations of which Namibia Power Corporation (Nam Power), Windhoek, Namibia is no exception. The locus of this study was to analyse the influence of talent management on organizational performance. The study aimed to analyse the impact of strategic talent management strategies on organisational performance at Nam Power, to determine the factors that hinder talent management in an organisation, and to design a model for the implementation of talent management programs that enhance organisational performance. The research philosophy underpinning this study is descriptive in nature and used to establish the association between the dependent and independent variables. Both quantitative and qualitative approaches were applied to collect the data. Some data was subjected to rigorous quantitative analysis through the use of questionnaires and managers were also interviewed to collect qualitative data. The collected data was analysed and synthesised using SPSS (Statistical Package of Social Science) software with multiple regression analysis. This software enabled the researcher to analyse data, visualize findings, and draw meaningful conclusions. The researcher used regression analysis to examine the relationship between the predictor variable and outcome. The data was analysed and displayed using bar graphs and pie charts in Microsoft Excel. The study revealed that the factors that hinder talent management in the organisation institution among others include a shortage of capacity building and professional development, absence of enthusiasm, and a shortage of professional development programs. It was recommended that to attract, develop, and maintain high-performing employees in the organisation, management should introduce increased salaries, create staff development programs, advocate for a clear communication channel between staff and leadership, and have a dynamic team of human resources professionals with competent HR systems and procedures. The study further recommends that for these programs to effectively yield good results, the organisation needs to design a talent management model that should include these components such as planning, attracting, developing, retaining, and transitioning.

The role of parental involvement in academic and sports achievement

According to previous research results and our systematic review on this topic (Kovács et al., 2022), the positive impact of parental involvement on academic achievement was revealed. However, it is unclear how parental involvement in sports contributes to sports performance and academic achievement. In this study, our main questions are about the differences in academic achievement, in participation in sports activity, and in sports results among pupils of parents involved or not involved in sports and education. To answer these questions, a questionnaire survey was conducted among 7th and 8th-grade students learning in three primary schools in a Hungarian city (N = 121). Based on the scale of parental involvement, three groups were differentiated: 1) children of parents not involved in either education or sports (N = 33), 2) children of parents involved in education only (N = 38), and 3) children of parents involved in both education and sports (N = 47). In order to examine this data, cluster and factor analysis, a Chi-square test and ANOVA, and linear regression were used. Our results showed, children of parents involved in both sports and education are overrepresented among students who received awards because of their sports performance (59.6%), among members of sports talent management programs (29.8%), and among those who achieved first place in national sports competitions (47.8%). They also seem to be the ones most intent on staying engaged, in the future, in regular and competitive sport activities (57.4% and 55.3%). On the contrary, the highest percentage of children of parents involved in education belongs to those who were given awards because of excellent academic achievement (71.1%). As far as personality traits are concerned, obsessive passion (OP) is the most characteristic of pupils with non-involved parents (M = 0.37, SD = 0.95) and least characteristic of children with parents involved in education (M = −0.17, SD = 0.94). Belonging to both groups with involved parents can be considered a negative predictor of OP (βt = −0.259* [−1.019, −0.078], βt + s = −0.237* [−0.930, −0.030]). In conclusion, it can be detected that there exists a positive interrelation between parental involvement in sports and education, and the academic and sports performance of their children.

자유전공학부 운영 현황 및 학생 특성 분석에 관한 연구

This thesis aims to guide the future development of the liberal studies system in Korean universities. It assesses the current state of universities with this system and analyzes student characteristics. The study delves into the concept of talent, educational goals, and main programs of liberal studies institutions in Korean universities. Additionally, it scrutinizes the talent concept, vision, and educational goals of I University's Liberal Studies Division, exploring concrete education programs. The research also examines traits of Liberal Studies Division students, surveying freshmen on motivation, satisfaction, participation, commitment, major decisions, and preferences. To establish the liberal studies system, the study suggests recognizing the tradition and value of liberal education, designing a systematic curriculum, implementing theme-centered classes for active student participation, and devising customized support strategies for rational major choices.

Introduction to the special issue: Addressing Complex Problems in Higher Education Talent Programs

Introduction to the special issue: Addressing Complex Problems in Higher Education Talent Programs

Revitalizing Talent Development in Jiangsu Province's Representative Universities under 'The Belt and Road' Initiative in the Post-Pandemic Landscape: A Data-driven Analysis

Examining four universities in Jiangsu Province's "Belt and Road" international talent programs, this study finds disparities in planning, faculty diversity, and student exchanges. Despite Jiangsu's reputation for excellence in higher education, these institutions perform in a range of ways, most notably in cooperation and planning within the initiative. Although some universities are excellent at internationalizing their faculty, it is still difficult to recruit from "Belt and Road" nations. The pandemic affected participation rates by interfering with exchange and student enrollment programs. Evaluating and correcting shortcomings in Jiangsu universities' "Belt and Road" talent programs was the main goal. With an emphasis on student exchanges, faculty compositions, and educational planning, the study sought to close gaps that prevent successful internationalization. The research sought to reconcile current practices with the urgent need for strong international talent development through careful analysis. Using a thorough methodology, the study examined student exchange data, faculty compositions, and educational plans, identifying notable differences. These results highlight the need for better planning, diverse faculty hiring, and equitable student exchanges. In summary, this study offers crucial recommendations to higher education institutions and decision-makers for improving the success of "Belt and Road" projects in the aftermath of the pandemic, stressing the importance of better international talent development and more robust global alliances.

A machine learning approach for the classification of sports based on a coaches’ perspective of environmental, individual and task requirements: A sports profile analysis

ABSTRACT Well-designed talent programmes in sports with a focus on talent identification, orientation, development, and transfer support the engagement of young individuals and the pursuit of elite performance. To facilitate these processes, an analysis of task, environmental and individual characteristics per sport is much needed. The aims of this study were to 1) analyse whether unique profiles per sport could be established by generic characteristics and 2) to discuss similarities and differences for the potential application in talent development and transfer. By means of a validated survey, 1247 coaches from 34 sports ranked 18 characteristics on importance to their sports (0 = not important − 10 = very important). To discriminate the responses per sport a Discriminant Analysis (DA) was carried out. To refine the DA-classification, Uniform Manifold Approximation and Projection (UMAP) with CatBoost classifier was performed. To test the performance of the CatBoost classifier-algorithm, a confusion-matrix was generated. The cross-validated DA showed that 70.2% of the coaches were correctly classified to their sport. The UMAP/CatBoost technique revealed 75.1% accuracy with correctly predicted responses per sport ranging from 18.2% (sailing) to 98.2% (soccer). With varying precision, the algorithm was able to differentiate sports by importance of its characteristics indicating similarities and differences per sport.

Gifted &amp; Talented Programs and Racial Segregation

Abstract Racial segregation can occur not only between schools but also within schools, and there has been particular concern that gifted &amp; talented (G&amp;T) programs may increase within-school segregation at the primary school level. This paper evaluates the contribution of G&amp;T education to racial segregation using data on the presence and racial composition of G&amp;T programs at virtually all U.S. elementary schools over a span of nine school years. I first show that, consistent with widespread perceptions, G&amp;T programs do disproportionately enroll white and Asian students while black, Hispanic, and Native American students are underrepresented. Next, I calculate the changes in the Dissimilarity and Exposure Indices that occur when G&amp;T programs are treated as fully separate schools, which provides a transparent measure of their contribution to overall racial segregation. These calculations indicate that accounting for the within-school racial sorting caused by G&amp;T education increases the Dissimilarity Index by 10 to 20 percent, and has little to no impact on the Exposure Index. Finally, to study the potential effects of G&amp;T programs on enrollments over time, I estimate event study models for schools initiating or discontinuing G&amp;T programs, and find no large changes in white or Asian enrollment after programs are eliminated or initiated. I conclude that G&amp;T education's impact on racial segregation in U.S. elementary schools is modest, although changes in G&amp;T programming could still be one meaningful tool to increase integration.

Talent mobility for accelerating diversity at leadership levels: A pilot study

Mayo Clinic is committed to reskilling and upskilling its workforce and to creating a strong, diverse leadership pipeline to advance its ‘Bold. Forward.’ strategy. In alignment with this commitment, the Mayo Clinic Shared Services Organization (MCSSO) launched a pilot offering staff the opportunity to work in a hybrid role and build new skills and connections. Pilot participants split their time equally between job roles in which the professional skill sets required were approximately the same. The pilot involved six MCSSO departments and six candidates (three pairs) from the participating departments. Staff were recruited for three roles: senior project manager, senior business analyst and call centre representative. Each of the three pairs worked in a hybrid assignment for six months. Candidates were offered opportunities to network with each other, MCSSO senior leaders and other diverse colleagues. Pilot evaluations were conducted at 0, 3 and 6 months with candidates and their supervisors. Additionally, benchmarking with external organisations allowed learning from advanced talent mobility and leadership diversity programmes. The evaluations highlighted that 50 per cent of the candidates were promoted by the end of their hybrid assignment. Most of them engaged in new projects, cross-trained and gained valuable insights, skills and expertise. Candidates and supervisors valued the opportunity to network with senior leaders and cross-functional colleagues. The pilot and external benchmarks emphasised the importance of a formal ‘talent mobility programme’ to build and sustain a diverse leadership pipeline. It is crucial to be intentional and bold with hybrid and mobility opportunities for intersectional candidates, under-represented groups and staff committed to equity, inclusion and diversity. All candidates and supervisors wanted the pilot to be expanded and operationalised. We believe that these early experiences, results and lessons on preparing diverse talent for leadership levels in the organisation are broadly transferable to other healthcare and non-healthcare organisations.

FABP4 Suppresses the Progress of Multiple Myeloma through Enhancing Alarming Function of Bone Marrow Resident Memory T Lymphocytes

Multiple myeloma (MM) is a hematological malignancy which characterized by malignant plasma cells resident in the bone marrow (BM). The BM microenvironment plays critical roles in the invasion, proliferation and migration of myeloma cells. Despite the therapies of MM improved in recent years, this disease is still incurable and requires precise treatment. Resident memory (TRM) cells are a distinct tissue-localized T cell lineage that is crucial for protective immunity in peripheral tissues. Recent studies reveal TRM cells as a vital component of the host immune response to cancer. TRM-like tumor-infiltrating lymphocytes (TILs) can be found in a wide range of human cancers, where they portend improved prognosis. However, the precise role and mechanism of bone marrow TRM cells in MM still remain elusive. In present study we first found that the accumulation of T RM cells (CD8 +CD44 +CD69 +CD62L -) in BM newly diagnosed MM (NDMM) patients was significantly higher than that of relapse recurrent MM (RRMM) ( Figure A), which was further confirmed by the immunohistochemistry (IHC) staining analysis ( Figure B). By using Seahorse XF e analyzer we found a decrease of oxygen consumption rate (OCR) of T RM cells in RRMM comparing to that of NDMM ( Figure C). Since fatty acid metabolism is important in β oxidation, we further investigated the expression of fatty acid binding proteins (FABP4/FABP5) in T RM cells and showed that FABP4 was dominant in T RM cells of NDMM patients ( Figure D). FABP4 +/+ mice and their relative controls FABP4 -/- mice were adopted to further explore the role and mechanism of T RM cells in MM progression. The abundance of genes related fatty acids β oxidation was significantly decreased in FABP4 -/- T RM cells ( Figure E). The tumor cell viability was decreased in myeloma cells co-cultured with FABP4 -/- T RM cells in the presence of bortezomib ( Figure F). Moreover, the expression of cytotoxic cytokines including IFN γ, TNFα and CXCL10 was significantly higher in FABP4 -/- T RM cells ( Figure G). The alarming function of T RM cells was further enhanced via recruiting more effector T (CD8 +CXCR3 +) cells ( Figure H), therefore the apoptosis of tumor cells was elevated in BM of MM patients. In summary, T RM cells are necessary and sufficient for long-lived protection against tumors. T RM cells derived FABP4 exerts anti-tumor immunity through prolonging the survival of T RM cells and enhancing the recruitment of more effector T cells to suppress the tumor progression ( Figure I). This study suggests that T RM cells might serve as a novel therapeutic target in MM and treatment specific targeting FABP4 might shed light on the development of potential therapeutic strategies for treating MM especially relapse patients. Acknowledgement This project is supported by the Sun Yat-sen University Hundred Talents Program (PT19200101), Natural Science Foundation of Guangdong Province (2022A1515010290).

Talent Development Programs for Secondary Schools: Implementation and Evaluation of a Model School

A school profile of talent development including model classes has been implemented at BG/BRG Keimgasse. This paper evaluates the impact of the actions taken by the school and compares the effects of both the model classes and the regular classes, with a school without a special focus on talent development. The aim of this study was to investigate the influence that the change in profile and teaching strategies had on both types of classes. This was conducted through initial qualitative interviews, followed up by quantitative questionnaires. It was found that the model classes had significantly higher scores in terms of school satisfaction, class climate, self-efficacy, mastery goal orientation, and in hope of successes, as well as scoring significantly lower on classroom pressure. This was achieved while shortening the education for the model-class students by one year and adding extracurricular activities. When comparing the regular classes at BG/BRG Keimgasse with classes from a regular school, the scores differed only slightly. This suggested that the concepts integrated at BG/BRG Keimgasse were successful in catering to the gifted students, without compromising the quality of the regular education.

Interphylum dissemination of NDM-5-positive plasmids in hospital wastewater from Fuzhou, China: a single-centre, culture-independent, plasmid transmission study

The global spread of plasmid-borne carbapenem resistance is an ongoing public health challenge; however, the nature of such horizontal gene transfer events among complex bacterial communities remains poorly understood. We examined the in-situ transfer of the globally dominant New Delhi metallo-β-lactamase (NDM)-5-positive IncX3 plasmid (denoted pX3_NDM-5) in hospital wastewater to simulate a real-world, One Health antimicrobial resistance context. For this transmission study, we tagged pX3_NDM-5 with the green fluorescent protein gene, gfp, using a CRISPR-based method and transferred the plasmid to a donor Escherichia coli strain. Bacteria were extracted from a hospital wastewater treatment plant (Fujian Provincial Maternity and Children's Hospital, Fuzhou, China) as the bacterial recipient community. We mixed this recipient community with the E coli donor strain carrying the gfp-tagged plasmid, both with and without sodium hypochlorite (NaClO) as an environmental stressor, and conducted several culture-based and culture-independent conjugation assays. The conjugation events were observed microscopically and quantified by fluorescence-activated cell sorting. We analysed the taxonomic composition of the sorted transconjugal pool by 16S rRNA gene amplicon sequencing and assessed the stability of the plasmid in the isolated transconjugants and its ability to transfer back to E coli. We show that the plasmid pX3_NDM-5 has a broad host range and can transfer across various bacterial phyla, including between Gram-negative and Gram-positive bacteria. Although environmental stress with NaClO did not affect the overall plasmid transfer frequency, it reduced the breadth of the transconjugant pool. The taxonomic composition of the transconjugal pool was distinct from that of the recipient communities, and environmental stress modulated the permissiveness of some operational taxonomic units towards the acquisition of pX3_NDM-5. Notably, pX3_NDM-5 transconjugants included the Gram-positive pathogen Enterococcus faecalis, and the plasmid could subsequently be reconjugated back to E coli. These findings suggest that E faecalis could act as a natural shuttle vector for the wide dissemination of pX3_NDM-5 plasmids. Our culture-independent conjugation model simulates natural environmental conditions and challenges the established theory that Gram-negative and Gram-positive bacteria rarely exchange clinically important plasmids. The data show that plasmids disseminate more widely across genera and phyla than previously thought. These findings have substantial implications when considering the spread of antimicrobial resistance across One Health sectors. The Laboratory of Lingnan Modern Agriculture Project, the National Natural Science Foundation of China, the Natural Science Foundation of Fujian Province of China, and the Outstanding Young Research Talents Program of Fujian Agriculture and Forestry University.