Syzygium Aromaticum Extract Research Articles

Determination of the minimum inhibitor concentration of Syzygium aromaticum extract on the growth of Pseudomonas aeruginosa isolated from burn infection

The cold aqueous extraction method of Syzygium aromaticum was used and the minimum inhibitory concentration (MIC) was measured after culturing the bacteria in a nutrient broth medium containing known concentrations of the extract. And the susceptibility of the bacteria to a group of common antibiotics has been determined.
 The results showed that 72.8% of the burn infection were caused by P. aeruginosa, and that the (MIC) value ranged between (0.01-0.1 mg / ml). And that the bacteria showed multi resistance to antibiotics, and high sensitivity to Nitrofurantoin.

Controlling Size Distribution of Silver Nanoparticles using Natural Reducing Agents in MCM‐41@Ag

AbstractIt is by reported the effect of natural reducing agents such as plant extracts (Equisetum myriochaetum leaves, Cymbopogon citratus, Camellia sinensis, Syzygium aromaticum), fruits (orange, apple), and biopolymers (arabic gum, and chitosan) on the size distribution of silver nanoparticles, obtained by green synthesis, to be incorporated in a MCM‐41@Ag nanocomposite. The aim is to determine the influence of temperature, agitation time, concentration, atmosphere, and nature of the reducing agent on the MCM‐41@Ag particle size. According to the DLS analysis, the extract of Syzygium aromaticum produced silver nanoparticles in the range of 8–80 nm. Further structural, morphological, optical, and surface characterization confirmed the obtention of the desired MCM‐41@Ag nanocomposite. Our results show the potential of MCM‐41@Ag to be incorporated in Metal‐insulator‐semiconductor capacitors for detection purposes.

Study of chloroquine susceptibility potential of plants using pseudomonas aeruginosa as in vitro model.

Chloroquine (CQ) is mainly known for antimalarial activity but due to lower sensitivity, it has not been well explored in the microbial disease treatment. In the present investigation, we attempted to enhance the CQ sensitivity in Pseudomonas aeruginosa. Presence of efflux pump is well demonstrated in bacterial system which plays an important role in drug sensitivity and resistance in bacteria and also serves other functions. Taking the advantage of presence of efflux pump in Pseudomonas aeruginosa, we made an attempt to sensitize the Pseudomonas aeruginosa with various plant extracts and phytochemicals for the development of CQ sensitivity. Ten rationally selected plant extracts were screened for the development of chloroquine sensitivity in P. aeruginosa. The chloroquine susceptibility assay was demonstrated by combining CQ and verapamil (a known efflux pump inhibitor) as a standard in an in vitro assay system. Results were quite encouraging as methanolic extracts of Syzygium aromaticum, Zingiber officinale and Curcuma longa were able to enhance chloroquine sensitivity in P. aeruginosa by increasing the zone of inhibition in well-defined assay system. These plant extracts were finally analysed for the presence of various phytochemicals. The Syzygium aromaticum extract showed the presence of phytochemicals, such as quinones, phenol, triterpenoid, saponins, tannins, alkaloids and flavonoids. On the other hand, the methanolic extract of Zingiber officinale and Curcuma longa showed the presence of saponins, tannins, alkaloids and flavonoids in the extract. Towards the identification of active principle of selected plant extract for CQ sensitivity enhancement, thin-layer chromatography was performed and various phytocomponent bands were isolated. Flavonoid (Rf 0.44) in Syzygium aromaticum, alkaloid (Rf 0.43) in Zingiber officinale and phenol (Rf 0.62) in Curcuma longa were found responsible for the enhancement of CQ susceptibility in P. aeruginosa. This interesting finding confirmed the concept that a prior course or combination of plant extracts or phytochemicals with chloroquine can be effective against P. aeruginosa. Present investigation successfully presented the proof of concept for the enhancement of chloroquine sensitivity in bacterial system by modulating an efflux pump. Concept can be explored for repurposing chloroquine for new applications.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13205-022-03382-1.

Effectiveness of Syzygium aromaticum Extract in Prevention of Diabetic Retinopathy in Experimental Animals

Aim of the study: Syzygium aromaticum has been reported to possess aphrodisiac, stomachic, carminative, antispasmodic and anti-cataract properties in traditional ayurvedic medicine. The current study determined the efficacy of S. aromaticum alcoholic extract in the prevention of diabetic retinopathy in rats with type 2 diabetes. Materials and Methods: Type 2 diabetes was induced in neonatal rats with streptozotocin. The effect of S. aromaticum alcoholic extract in different doses on glycemic parameters, lenticular changes, vessel diameter, antioxidant enzymes, inflammatory markers and thickness basement membrane of retinal capillaries was assessed with electron microscopy. The results of the treatment groups was compared with that of the diabetes control group. Results: The treatment of diabetic rats with S. aromaticum alcoholic extract at different doses significantly reduced blood glucose, glycated hemoglobin, lenticular changes, vessel diameter, inflammatory markers and thickness of basement membrane of retinal capillaries and antioxidant enzymes status. Conclusion: S. aromaticum alcoholic extract may be used strategically to prevent diabetic retinopathy.

Study on Antioxidant Property of Syzygium aromaticum (Clove)

This paper is aimed at evaluating the antioxidant properties of Eugenia Caryophyllata (Syzygium aromaticum), 100% aqueous and 20:80% (water and alcohol) for hydroethanolic and hydromethanolic extracts were prepared as working solutions for the studies. About 5 different antioxidant examinations of Syzygium aromaticum (Clove) extracts were carried out which include total phenolic content, antioxidant assay, free radical’s DPPH scavenging activity, scavenging hydrogen peroxide and reducing power assay, which was estimated spectrophotometrically by the use of butylated hydroxyanisole (BHA) as standard, and tocopherol used as the standard for reducing power test. Hydromethanolic extract exhibited higher free radical scavenging activity (62.12%), at the highest concentration of 1000 µg/mL followed by hydroethanolic and aqueous (58.80% and 48.32% respectively), less reducing power properties were observed with high absorbance value in the highest concentration of hydromethanolic extract (0.198 ± 0.001 A) and also hydromethanolic extract shows highest scavenging hydrogen peroxide (76.99 ± 0.09).

Inhibition of Escherichia coli nitroreductase by the constituents in Syzygium aromaticum.

Gut bacterial nitroreductases play an important role in reduction of various nitroaromatic compounds to the corresponding N-nitroso compounds, hydroxylamines or aromatic amines, most of which are carcinogenic and mutagenic agents. Inhibition of gut nitroreductases has been recognized as an attractive approach for reducing mutagen metabolites in the colon, so as to prevent colon diseases. In this study, the inhibitory effects of 55 herbal medicines against Escherichia coli(E. coli) nitroreductase (EcNfsA) were examined. Compared with other herbal extracts, Syzygium aromaticum extract showed superior inhibitory potency toward EcNfsA mediated nitrofurazone reduction. Then, the inhibitory effects of 22 major constituents in Syzygium aromaticum against EcNfsA were evaluted. Compared with other tested natural compounds, ellagic acid, corilagin, betulinic acid, oleanic acid, ursolic acid, urolithin M5 and isorhamnetin were found with strong to moderate inhibitory effect against EcNfsA, with IC50 values ranging from 0.67 to 28.98 mol·L-1. Furthermore, the inhibition kinetic analysis and docking simulation demonstrated that ellagic acid and betulinic acid potently inhibited EcNfsA (Ki < 2 μmol·L -1) in a competitively inhibitory manner, which created strong interactions with the catalytic triad of EcNfsA. In summary, our findings provide new scientific basis for explaining the anti-mutagenic activity of Syzygium aromaticum, where some newly identified EcNfsA inhibitors can be used for developing novel agents to reduce the toxicity induced by bacterial nitroreductase.

Phyto-mediated synthesis of Pt and Au/Pt bimetallic nanoparticles using Syzygium aromaticum bud-extract: Study of their catalytic, antibacterial, and antioxidant activities

Herein, we report bio-mediated production of Pt nanoparticles and Au/Pt BMNPs using aqueous extract of Syzygium aromaticum (clove) buds. The presence of phenolic compounds, mainly eugenol was responsible for reduction of metal ions as confirmed through FTIR spectroscopic analysis. The appearance of single SPR band in UV–Visible spectra revealed the alloy nature of BMNPs. Further, microscopic analysis showed the dependence of concentration, pH and temperature on morphology and size of the particles. Other techniques such as XRD, XPS, Zeta potential and cyclic voltammetry were employed to scrutinize the structural and compositional features of the NPs. Au/Pt BMNPs showed good anti-oxidative potential by inhibiting DPPH (IC50-27.2 μg/mL) and ABTS (IC50-14 μg/mL). The antibacterial activity was also investigated, and the zone of inhibition was maximum for Au/Pt BMNPs against Gram negative (Pseudomonas aeruginosa; 18 mm) and Gram positive (Staphylococcus aureus; 16 mm) bacteria. Additionally, Au/Pt BMNPs (96% degradation in 6 min) exhibited better catalytic efficiency for the reduction of PNP compared to monometallic Pt NPs (92% degradation in 10 min). In conclusion, our results reveal better potential efficacy of Au/Pt biosynthesized BMNPs due to the synergistic interactions between monometallic components.

Changes in Quality Traits and Oxidation Stability of Syzygium aromaticum Extract-Added Cooked Ground Beef during Frozen Storage.

This study was accomplished by comparing the oxidative stability of (0.1%) Syzygium aromaticum extract (SAE) and (0.02%) butylated hydroxytoluene (BHT)-added cooked ground beef with an antioxidant free-control sample during frozen storage. All samples showed a non-significant (p > 0.05) effect on pH, thawing loss, redness, and yellowness values during storage. Incorporation of BHT and SAE led to a significant (p < 0.05) reduction in thiobarbituric acid-reactive substances (TBARS) and volatile levels as an active antioxidant. The generation of less volatiles found in SAE-treated samples up to 6 months (p < 0.05) of storage. Therefore, SAE-protected ground beef can lead to lower lightness, lipid oxidation, and volatile compounds levels after cooking compared with control and BHT samples.

Ameliorative role of Syzygium aromaticum aqueous extract on synaptosomal tyrosine hydroxylase activity, oxidative stress parameters, and behavioral changes in lead-induced neurotoxicity in mice.

This study reports the protective role of the aqueous extract of Syzygium aromaticum (ESA) against lead (Pb)-induced neurotoxicity in mice. Thirty male mice weighing between 18g and 25g were randomly divided into five groups. (1) Group 1 (control group), (2) group 2 (Pb-test group): was administered with a solution containing 0.1% (w/v) of lead acetate (PbAc), (3) group 3 (ESA+Pb100 group): was administered with 0.1% (w/v) of PbAc followed by 100mg/kg of S. aromaticum extract by gavage, (4) group 4 (ESA+Pb200): was administered with 0.1% (w/v) of PbAc followed by 200mg/kg of S. aromaticum extract, and (5) group 5 (ESA-group): was administered with 100mg/kg of S. aromaticum. Level of lead was determined by atomic absorption spectroscopy. Cerebral cortex synaptosomes prepared from mice administered orally with lead-acetate shown a significantly increased (p<.05) in tyrosine hydroxylase and protein carbonyl level and significantly decreased (p<.05) superoxide dismutase, glutathione reductase, and glutathione transferase activities. Also, there was a significant increase in brain lead concentration level, however, it was observed that S. aromaticum significantly reduced (p<.05) the level of lead at all tested doses. S. aromaticum rescued cerebral cortex synaptosomes from lead-induced neurotoxicity by relieving oxidative stress and abating elevated tyrosine hydroxylase activity. Moreover, S. aromaticum at the different dose grade (100mg and 200mg) abrogated the loss of motor performance in mice groups induced with lead. Altogether, our findings showed that S. aromaticum possesses antioxidant and neuro-modulatory potential against lead-induced neuronal damage. PRACTICAL APPLICATIONS: Environmental pollution with heavy metals is a known public health concern and their incremental concentrations in soil and water have risen to an unprecedented degree. Lead is one of the top 10 contaminants on the WHO's list of substances of greatest public health concern that impact the brain. However, exogenous natural bioactive supplements molecules could be one of the remedies to reduce Pb-induced toxicity. Our findings indicate therefore that, S. aromaticum could be a good fit for lowering Pb neurotoxicity and could be suggested as a neuroprotective molecule against neurodegenerative diseases involving catecholaminergic dysfunction induced by metallic elements.

Investigation of Syzygium Aromaticum and Nicotiana Tabacum Extracts as Corrosion Inhibitor

In the present work, corrosion inhibition of Syzygium aromaticum and Nicotiana tabacum extract was studied by DFT method. Frontier molecular orbitals (FMO) plots, quantum chemical descriptors such as highest occupied molecular orbital energy (EHOMO), lowest unoccupied molecular orbital energy (ELUMO), energy gap (ΔE), dipole moment (l) and partial charge analysis were calculated. The results obtained show that the eugenol as extract of Syzygium aromaticum and nicotine as extract of Nicotiana tabacum acts as an effective green corrosion inhibitor. In general, nicotine has a better potential as an green corrosion inhibitor than eugenol based on chemical reactivity. However, further studies are needed to gain insight into the mechanism of corrosion inhibition through the study of the adsorption of eugenol and nicotine molecules on metal surfaces.

Fabrication and enzyme-free electrochemical sensing response of polyaniline/silver/clove modified composite electrode

The sensitive detection of Hg(II) is a huge consequence for safe environment monitoring and industrial processing. Herein, a sensitive and environment-friendly electrochemical sensor was fabricated using Ag nanoparticles anchored Pani-based bio-nanocomposite. In a simple one-step process AgNPs were prepared using Syzygium aromaticum extract followed by incorporating polyaniline nanostructural array (Pani/Ag/CL) at room temperature through in-situ oxidative polymerization using K2S2O8. The glassy carbon electrode (GCE) was modified with Pani/Ag/CL for the selective electrochemical determination of trace amounts of Hg(II) from different aqueous samples. The electrochemical sensing behavior of the Pani/Ag/CL modified GCE was examined through cyclic voltammetry. The electrochemical impedance analysis of Pani/Ag/CL modified GCE displays favorable kinetic charges for transport. The best reactivity performance for electro-catalytic reduction reactions was observed in a phosphate buffer solution of pH = 5.7. The proposed PANI/Ag/CL modified GCE attains a high sensitivity of 256 μA μM−1 cm−2, a very low limit of detection (0.382 nM), and the limit of quantification (1.27 nM). The practical feasibility and selectivity of the PANI/Ag/CL/GCE were investigated in the sea, tap and wastewater samples with the adequate recovery of Hg(II) added. In addition, the proposed Pani/Ag/CL modified GCE also displayed attractive features such as fast response, simple preparation, repeatability, good stability, as well as detection of the Hg(II) in the occurrence of potential interferences metal cations.

Development of plant based nanoemulsion and its application as natural preservative having antioxidant and antimicrobial properties to deliver active compounds in apple juice

In this study, active compound loaded stable nanoemulsion (surface charge -18 mV) was developed having small diameter (&lt;100 nm) from the plant extract of raw Syzygium aromaticum , Ocimum tenuiflorum and rhizome of Zingiber officinale . The antimicrobial and antioxidant properties of formulated nanoemulsion were analyzed. The nanoemulsion showed good antimicrobial activity against various food poisoning organisms viz. bacteria ( Staphylococcus aureus and Escherichia coli ) and fungi ( Candida albicans ). For the antioxidant study, two different methods 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic 167 acid) diammonium salt (ABTS) were used, and a satisfactory result achieved (% inhibition for DPPH: 63.64±1.85 and ABTS: 48.36±1.12). The nanoemulsion in combination with a low dose of benzoic acid (200 ppm) is used as a preservative in formulated apple juice. The formulated juice quality was analyzed compared with standard fruit juice during a storage period of 30 days and quality of fruit juice remained good. This method thus allows reducing the use of synthetic preservatives with added benefit of natural beneficial compound in the formulated juice for human consumption.

Biogenic synthesis of biocompatible L-lysine coated hematite NPs for seed germination

Utilization of nanoparticles (NPs) in various biomedical fields, is of enhancing interest. Nonetheless their use in clinical practices is limited due to low uptake efficiency and toxicity towards the cells. The key factors affecting the toxicity and biocompatibility are the bio-physical properties of NPs comprising size, shape, surface charge and composition. Therefore, alteration in the surface properties of NPs leads to improved biocompatibility of NPs. In the present work, the effect of amino acid coating on the characteristics and biocompatibility of bare iron oxide NPs (c-Fe2O3) has been studied. L-lysine coated iron oxide NPs (Ly-Fe2O3) where synthesized by green route using plant extract of Syzygium aromaticum and their characteristics were compared to those of bare c-Fe2O3 NPs. The outcomes showed that amino acid coating on c-Fe2O3 NPs enhanced the particle stability and biocompatibility of NPs without affecting their core properties like morphology and crystallinity.

Larvicidal activity and Histopathological changes of Cinnamomum burmannii, Syzygium aromaticum extracts and their combination on Culex pipiens

In order to develop an eco-friendly botanical larvicide alternative to the synthetic larvicides, extracts were prepared from the Cinnamomum burmannii (C.B.) and Syzygium aromaticum (S.A.) with hexane using a sonicator. The extracts were evaluated for larvicidal activity individually and in combination against the Culex pipiens larvae. The LC50 value of C.B. and the S.A. hexane extracts tested individually were 184.2 and 363.7 µg/mL against Cx. pipiens respectively. All the combinations of the extract of C.B. and S.A. showed synergistic factors higher than one. Among the different ratios of extracts, the SA25%: CB75% extract was found to be more toxic than the other combinations (LC50:125.7 µg/mL). Midgut cells treated with S.A. 25%: C.B. 75% extract showed severe morphological alterations such as degradation of microvilli; degeneration of epithelial cells, and peritrophic membrane; loss of nuclei, irregular and damage of microvilli. The extract has a promising larvicidal potential against Cx. pipiens, However, the extract was toxic against HUVEC cells, as evident from MTT and cell morphology. Further investigation is required to assess the toxicity of the extract on aquatic animals.

UJI EFEKTIVITAS FUNGISIDA NABATI SEBAGAI BAHAN PENGAWET UNTUK MENCEGAH SERANGAN JAMUR PEWARNA KAYU

Woods which have low natural durability are easy to be attacked with wood staining fungi (blue stain) so can decrease its wood quality. Syzygium aromaticum, Melia azedarach and Piper betle contain of active components (botanical fungicide) that can be used as natural preservative to prevent staining fungi attack. The purpose of this research is to know effect of botanical fungicide species and preserved wood investigated, i.e Paraserianthes falcataria and Toona sureni on the attack intensity of wood staining fungi. Results of the research showed that interaction between botanical fungicide and wood species gived very significant effect on the attack intensity of wood staining fungi. The lowest of attack intensity was resulted on Toona sureni that had preserved by Melia azedarach leaf extract. The leaf extract of Syzygium aromaticum, Melia azedarach and Piper betle could keep Paraserianthes falcataria and Toona sureni wood against staining fungi. Toona sureni wood was more resist against wood staining fungi than Paraserianthes falcataria, it was due to higher extractive in Toona sureni than Paraserianthes falcataria. To preserve woods which had bright colour such as Paraserianthes falcataria, more suitable to use Melia azedarach leaf extract which not change wood colour. &#x0D; Key words: Botanical Fungicide, Preservative, Wood Staining Fungi

Screening of antimicrobial efficacy of traditionally used Indian plants against microorganisms associated with dandruff

Dandruff is a clinical condition affecting the scalp causing itching and relapsing inflammation. Staphylococcus epidermidis, Propionibacterium acne and Malassezia furfur pathogens are found more and less respectively in the scalps of dandruff subjects. In the present study, a total of 32 plant extracts were screened for their anti-dandruff activity by agar well diffusion method. Among the selected plant extracts of methanol and ethyl acetate, 25 showed significant activity while 7 extracts have not showed activity at a particular concentration. Among all the extracts ethylacetate extract of Cinnamomum zeylanicum and Glycyrrhiza glabra showed very high activity i.e. 18-20 mm while methanolic extract of Punica granatum and Syzygium aromaticum showed moderate activity against all the three pathogens. Thus the active plant extracts can be a potential source for the formulation of natural anti-dandruff agents.

Anticancer and antioxidant activity of ethanolic extract of Clove (Syzygium aromaticum) on oral squamous cell carcinoma cell lines (KB cell lines)- An In-vitro study

Cloves (Syzygium aromaticum) as been used as traditional medicine for many years and they possess antibacterial, antifungal and antiviral properties. Clove is known for its anticancer property on various cancer cell lines and is well established, but its anticancer effect on OSCC cell lines is less known. Aim of the study was to determine the anticancer and antioxidant effect of Syzygium aromaticum extract on OSCC cell lines (KB cell lines) and compare the same with normal mouse fibroblasts cell lines (L292 cell lines). KB cell lines and L292 cell lines were commercially obtained. Clove was obtained from local market and ethanolic extract (EC) of clove was prepared. Anticancer activity was assessed by MTT, neutral red, DAPI and Double staining assay and antioxidant assay was carried out by FRAP, PM and DPPH assay. The antioxidant property of EC of clove increased with increase in the concentration in a dose dependent manner. Both MTT and Neutral Red assay showed increase in cell death with increase in concentration of EC of clove. Double staining and DAPI showed increase in cell death when treated with EC of clove. The anticancer and antioxidant activity of EC of clove was comparable with standard drug used in the assay. This in vitro study demonstrates effective anticancer and antioxidant activity on KB cell lines when compared to standard control. However, further studies are to be conducted in order to characterize other potential antitumor components of the clove, so that it can be used as therapeutic agent in treating oral carcinoma.

Syzygium aromaticum Extracts as a Potential Antibacterial Inhibitors against Clinical Isolates of Acinetobacter baumannii: An In-Silico-Supported In-Vitro Study

Imipenem is the most efficient antibiotic against Acinetobacter baumannii infection, but new research has shown that the organism has also developed resistance to this agent. A. baumannii isolates from a total of 110 clinical samples were identified by multiplex PCR. The antibacterial activity of Syzygium aromaticum multiple extracts was assessed following the GC-Mass spectra analysis. The molecular docking study was performed to investigate the binding mode of interactions of guanosine (Ethanolic extract compound) against Penicillin- binding proteins 1 and 3 of A. baumannii. Ten isolates of A. baumannii were confirmed to carry recA and iutA genes. Isolates were multidrug-resistant containing blaTEM and BlaSHV. The concentrations (0.04 to 0.125 mg mL−1) of S. aromaticum ethanolic extract were very promising against A. baumannii isolates. Even though imipenem (0.02 mg mL−1) individually showed a great bactericidal efficacy against all isolates, the in-silico study of guanosine, apioline, eugenol, and elemicin showed acceptable fitting to the binding site of the A. baumannii PBP1 and/or PBP3 with highest binding energy for guanosine between −7.1 and −8.1 kcal/mol respectively. Moreover, it formed π-stacked interactions with the residue ARG76 at 4.14 and 5.6, Å respectively. These findings might support the in vitro study and show a substantial increase in binding affinity and enhanced physicochemical characteristics compared to imipenem.

CHARACTERIZATION OF GOLD NANOPARTICLES FROM CLOVE FLOWER WATER EXTRACT AND ITS ANTIOXIDANT ACTIVITY

CHARACTERIZATION OF GOLD NANOPARTICLES FROM CLOVE FLOWER WATER EXTRACT AND ITS ANTIOXIDANT ACTIVITY. Green chemistry is a safe and cost effective method for producing metal nanoparticles, characterize and tested the antioxidant activity of gold nanoparticles (AuNPs). In this research gold nanoparticles were synthesized using HAuCl₄ as the gold precursor and water extract of Syzygium aromaticum (L.) flowers 0.5% as the bioreductor. Characterization of the gold nanoparticles was carried out using UV-Vis spectrophotometer to measure the maximum wavelength, zeta analyzer to determine the particle size, and SEM EDS to investigate the morphology and content of the gold nanoparticles. The antioxidant activity was determined by measuring its free radical scavenging activity using the DPPH method. The research found the AuNPs has a maximum wavelength at 533 nm, and particle size of 73.52 nm. Furthermore, the AuNPs show free radical scavenging activities of up to 70%.

Regeneration of Pancreas Β-Cells in Alloxan-Induced Diabetes Rats Treated with Aqueous Seed Extract of Syzygium aromaticum: A Preliminary Study

Aim: The present study was conducted to investigate the preventive/curative effect of aqueous seed extract Syzygium aromaticum in alloxan-induced diabetes in rats.&#x0D; Study Design: Twenty Wistar rats were randomly divided into four groups. The first group served as a control (received 1 ml of normal saline). The second group served as Diabetic control (received 1 ml of normal saline). The third group served as test 1 (pre-treated with 500 mg/kg body weight (b.w.) of aqueous Syzygium aromaticum seeds extract for seven days prior to diabetic induction and post-treated with aqueous seeds extract of Syzygium aromaticum after diabetic induction) and the fourth group served as test 2 (post-treated with 500 mg/kg b.w. of aqueous seed extract of Syzygium aromaticum after diabetic induction) in a daily oral dose for 14 days.&#x0D; Results: Treatment of rats with aqueous seed extract of Syzygium aromaticum in a daily dose of 500 mg/kg significantly mitigates the induced changes in the glucose and lipid profile parameters. Histopathological examination of the pancreas showed the destruction of β-cells in the diabetic control group and recovery of damaged tissues when treated with 500 mg/kg b.w. of aqueous seeds extract of Syzygium aromaticum.&#x0D; Conclusion: The present study suggests that aqueous seed extract S. aromaticum seeds extract at a dose of 500 mg/kg body weight brings about regeneration of the B-cells of the pancreas and significant beneficial effects in various physiological parameters in alloxan-induced diabetes.