Regional innovation systems in Central and Eastern Europe suffer from many handicaps connected with generally lower levels of business activity and unfavourable positions of firms within global value chains. In this paper, results of business coaching programmes implemented in South Moravia (Czechia) are evaluated. The success of support is assessed (1) in terms of turnover, value added and employment growth (using a counterfactual analysis), and (2) according to the maturity of companies. Average annual growth of the treated companies was higher compared to a control group of non-supported firms. Apart from the initial dynamic growth, the supported start-ups underwent the fastest growth between 2 and 3 years after entering the programme, when the impact of the support on their performance was fully expressed. The findings bring new insights into the effects of targeted support of companies, using unique company data combined with qualitative information on the results of evaluated measures.