An inventory of the fishes of the Selva El Ocote Biosphere Reserve and Nezahualcóyotl (Malpaso) dam, including the Totopac River in Chiapas, Mexico, is presented. The material was collected from 14 sampling campaigns in 83 points between March 2007 and September 2009. Four more samplings were performed in 2014. In total 22,034 specimens were collected, the systematic list of the fishes comprises 47 species contained in 30 genera, 16 families and 10 orders. The most representative families were Cichlidae and Poeciliidae with 16 and 11 species respectively (57% of collected individuals). Six species are under some risk category according to NOM-059-SEMARNAT-2010, the IUCN Red List and the American Fisheries Society: Potamarius nelsoni, Rhamdia guatemalensis, Rhamdia laluchensis, Priapella intermedia, Xiphophorus clemenciae, and Paraneetroplus hartwegi. Four species are exotic: Oreochromis mossambicus, Oreochromis niloticus, Parachromis managuensis, and Tilapia zillii.