Detector System Research Articles

Abstract 1042: Multi-exposure high dynamic range microscopy obviates the requirement for matching camera exposure to reagent concentration in high-plex spatial biology applications

Abstract High-plex immunofluorescence imaging allows researchers to perform deep cellular phenotyping with spatial context. However, high-plex immunofluorescence imaging requires the end user to painstakingly optimize antibody concentrations and image acquisition settings before producing reproducible imaging data with sufficient and quantifiable dynamic range. Mismatches of reagent concentration and camera exposures will result in under and over-saturated images, and both outcomes are detrimental for signal quantification and downstream interpretation. As the number of markers deployed in spatial biology increases, it becomes necessary to develop strategies that streamline or automate parameter setting that is needed to capture data with optimal dynamic range. The CellScape™ platform for Precise Spatial Multiplexing, featuring ChipCytometry™ technology, utilizes multi-exposure high dynamic range (HDR) microscopy as a tool to produce data with consistent and reliable detection of biomarker signals. CellScape replaces the standard single exposure with an exposure series calibrated to the physical limitations of the detector system. Each exposure series captures linear data, ranging from the dimmest to the brightest signals that the system can detect, as well as everything in between. HDR capture is thus akin to capturing an image at every possible exposure length, and it thus includes data from the ‘optimal’ matched exposure. By these means, HDR obviates the need for exposure selection by covering the entire exposure gamut. Here, we use CellScape to collect a high-plex immunofluorescence spatial biology dataset. Analysis of the dataset highlights the extended linear dynamic range of the integrated luminance HDR data, including a calculation of the expected ‘best-matched’ exposure. Further, we extend this analysis to show how HDR can handle biological variation and allow normalization that is not possible with single exposure imaging. Finally, we examine a reagent concentration series to demonstrate how HDR can be used to maximize the sensitivity of the assay by allowing increasing detection reagent concentration without compromising overall dynamic range for the signal. Our data demonstrates that HDR microscopy supports the deployment of spatial biology as a tool to interrogate the tumor microenvironment, unravel responsiveness to drug treatments and cell therapies, and ultimately stratify patients for targeted treatment and clinical trial enrollment. Citation Format: J Spencer Schwarz, Matthew H. Ingalls, Xenia Meshik, Adam Northcutt, Oliver Braubach. Multi-exposure high dynamic range microscopy obviates the requirement for matching camera exposure to reagent concentration in high-plex spatial biology applications [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1042.

Characterization of tracks from alpha-particles in PADC detectors for passive environmental monitoring of fast neutron radiation field

Fast neutron dosimetry is particularly crucial in the realm of radioprotection, as it significantly contributes to evaluating the radiation protection measures. This assessment aims to ensure and enhance the effectiveness of implemented safeguards and precautions, thereby minimizing the risks and potential harm associated with radiation exposure. Given the substantial biological damage that fast neutrons can inflict when interacting with living tissues, accurate dosimetry is indispensable for ensuring the safety of personnel in these environments and for optimizing radiation therapy treatments. Employing an indirect detector technique, this research focuses on characterizing the geometrical and optical properties of tracks produced by alpha particles resulting from boron-neutron reactions and distinguish them from the background made up of tracks generated from radon decay and impurities on the surface of the detector. This methodology holds potential utility particularly in situations where dosimeters are not adequately stored: while the idea of employing plastic or alternative materials to shield dosimeters from radon may appear straightforward, there are numerous factors complicating its effectiveness and universal applicability, for example when the radon proof encapsulations are not sealed perfectly. Through the development of a robust protocol for fast neutron dosimetry, we can not only differentiate between tracks produced by alpha particles of varying energy levels but also quantify the dose resulting from exposure to the neutron field. A solid-state nuclear track detector system (Politrack, Mi.am, Italy) was used to address the critical need for measuring exposure to fast neutrons, thermalized by polyethylene spheres. This advancement facilitates the implementation of more effective radioprotection strategies and contributes to the overall safety of radiation therapy procedures.

Polarization of Compton-Scattered Prompt Gamma-Ray Technique for HEU Detection at 186 keV

Prompt gamma-ray polarization is a practical method for detecting highly enriched uranium (HEU) in concealed sources. It also provides information on their geometry, magnetic fields, and radiation mechanisms. However, prompt gamma-ray polarization measurements have rarely been applied in nuclear nonproliferation areas to detect HEU. In this study, the feasibility of detecting the characteristic energy peak of 186 keV, which is associated with the asymmetry of the activation mechanism and the detection of energy-dependent polarization from concealed HEU sources, was evaluated using the Compton scattering approach. A Monte Carlo N-particle transport code simulation was used to realize the activation mechanism of HEU via two 1.4-mm strips of converter material [i.e., cesium lead tribromide (CsPbBr3)], transported by secondary scattered gamma rays during the three-stage process of Compton scattering, polarization, and detection. This paper presents the mathematical model, the physics of Compton scattering, and the polarization mechanism for the detection technique. In this case, the physics is relevant to both processes in which the emitted secondary scattered gamma rays undergo initial orthogonal polarization. Specifically, to meet the objective of testing the technical protocol for the enhanced detection of energy peaks associated with HEU, particularly 186 keV, simulations were conducted to quantify the HEU volume and neutron source strength in the MCNP data card to perform error analysis. The detector system had the potential to acquire good resolved photopeak with a 4.5% relative error or less, with a 1 Ci source activity, and a peak-to-background ratio of 1.15. This resolution took 163 s for high-purity germanium detection, which is comparable to current methods used for material detection placed within 100 to 900 s to completion. The small error difference was due to the attributes of the phenomenal enhancement properties of cesium tribromide and polarimetry. The identified photo peaks included K-shell X-rays from 235U, 61 keV from fission, 511-keV annihilation, and the peak of interest at 186 keV. The result from spectral analyses showed clear signatures related to pure and adulterated HEU. HEU detection with the low neutron yield and the easiness of shielding the yield of the HEU sample showed that the HEU characterization was feasible when shielded, with the highest success rate under both enhancement approaches. The optimization and scale-up of this technique are expected to enhance its applications in a large-scale HEU detection design.

Dazzling Evaluation of the Impact of a High-Repetition-Rate CO2 Pulsed Laser on Infrared Imaging Systems.

This article utilizes the Canny edge extraction algorithm based on contour curvature and the cross-correlation template matching algorithm to extensively study the impact of a high-repetition-rate CO2 pulsed laser on the target extraction and tracking performance of an infrared imaging detector. It establishes a quantified dazzling pattern for lasers on infrared imaging systems. By conducting laser dazzling and damage experiments, a detailed analysis of the normalized correlation between the target and the dazzling images is performed to quantitatively describe the laser dazzling effects. Simultaneously, an evaluation system, including target distance and laser power evaluation factors, is established to determine the dazzling level and whether the target is recognizable. The research results reveal that the laser power and target position are crucial factors affecting the detection performance of infrared imaging detector systems under laser dazzling. Different laser powers are required to successfully interfere with the recognition algorithm of the infrared imaging detector at different distances. And laser dazzling produces a considerable quantity of false edge information, which seriously affects the performance of the pattern recognition algorithm. In laser damage experiments, the detector experienced functional damage, with a quarter of the image displaying as completely black. The energy density threshold required for the functional damage of the detector is approximately 3 J/cm2. The dazzling assessment conclusions also apply to the evaluation of the damage results. Finally, the proposed evaluation formula aligns with the experimental results, objectively reflecting the actual impact of laser dazzling on the target extraction and the tracking performance of infrared imaging systems. This study provides an in-depth and accurate analysis for understanding the influence of lasers on the performance of infrared imaging detectors.

Simulation of Readout Electronics for Nuclear Radiation Detector Using MATLAB Program

A computer program was developed using the MATLAB programming language to simulate the electronics readout for a radiation detector system. The function of each stage of this system is described by a mathematical model in the Laplace domain. The electrical signals have been shown and analyzed at two main outputs of the system. They are described according to their related circuit parameters. The obtained results of the simulation can be used to achieve a best design of the concerned circuits and to provide appropriate details about the system operation. Validation of the simulated signals by comparison with available experimental results has been achieved.

Design and evaluation of a calibration system for portable motor vehicle emission particle number detectors

The supervision of the number concentration of particles emitted from motor vehicles is becoming increasingly strict. The regular calibration of portable particle number (PN) detectors is a prerequisite to ensure the effective supervision of PN. At present, the calibration of PN detectors is mostly completed in the laboratory. However, due to the complexity and poor portability of the laboratory calibration system, it is difficult to use this system for the on-site calibration of PN detectors. To this end, a portable motor vehicle emission PN detector calibration system based on the principles of negative pressure siphon atomization, differential electrical mobility classifier, and fA-level weak current measurement was developed, which could quickly and accurately calibrate the PN detector on-site. The system consists of three parts: an atomizer generator, a plate differential mobility analyzer (PDMA), and an aerosol electrometer. The atomizer generator produces NaCl particles with a median particle size of 70 nm and fluctuation of <10%. The particle size classification deviation of the PDMA is <3.7% and the counting error of the aerosol electrometer is <10%. The On-board Emissions Measurement System was calibrated as a PN detector on a self-developed portable calibration system as well as on a laboratory calibration system. The comparison results showed that the measurement results of the portable calibration system were closer to the instrument factory report certificate results, and the maximum error did not exceed 6.72%.

The LSU-Argonne conversion electron spectrometer: A new detector for the X-Array and SATURN decay station

A new conversion electron detector has been commissioned at the ATLAS/ CARIBU facility at Argonne National Laboratory. The LSU-Argonne Conversion Electron Spectrometer (LACES) is a LN2-cooled Si(Li) detector system designed to be incorporated into a decay station that comprises the dedicated HPGe clover array with a box geometry (X-Array) and the Scintillator and Tape Using Radioactive Nuclei (SATURN) device. This integration enables simultaneous measurements of conversion electrons and gamma-rays in decay experiments, yielding novel information on transition multipolarities, electric monopole transitions, and isomeric states that decay mostly via conversion electrons. A measurement of the energy resolution of LACES yielded 2.3-keV FWHM at 975 keV for electrons and 1.3-keV FWHM at 75 keV for X-rays. A detailed study of the absolute detection efficiency (at 5 mm from the source) was performed, where this quantity was determined experimentally in the range of electron transition energies between 25.5 keV and 1047.8 keV and subsequently simulated using the GEANT4 code. Measurement and simulations are found to be in excellent agreement. A precise characterization, for this type of detector system, of the absolute detection efficiency for such a wide energy range is reported for the first time.

Progress of Lyman-alpha-based beam emission spectroscopy (LyBES) diagnostic on the HL-2A tokamak

An edge Lyman-alpha-based beam emission spectroscopy (LyBES) diagnostic, using a heating NBI (neutral beam injection) system, is currently under development on the HL-2A tokamak. The 20-channel edge LyBES, which is intended to measure the density fluctuation in plasma edge (from R = 1960 mm to R = 2026 mm) with an improved spatial resolution of 3.3 mm, is a complement to the existing conventional beam emission spectroscopy (BES) diagnostic. In this article, we introduce the progress of LyBES diagnostic, including the collection optics, the monochromator, and the detector system. The reflectance of the collection mirrors is measured to be ~82% at 122 nm, and the aberration geometrical radius of the collection optics is tested to be ~150 μm in the aimed area. The linear dispersion of the LyBES monochromator is designed to be ~0.09 nm mm−1. The bandwidth of the detector system with the 5×107 V A−1 preamplifier gain is measured to be ~280 kHz, and the peak-to-peak noise of the detector system is tested to be ~16 mV. The finalized design, components development and testing of the LyBES diagnostic have been completed at present, and an overall performance of the LyBES diagnostic is to be confirmed in the next HL-2A campaign.

Molecular sieve vacuum swing adsorption purification and radon reduction system for gaseous dark matter and rare-event detectors

In the field of directional dark matter experiments SF6 has emerged as an ideal target gas. A critical challenge with this gas, and with other proposed gases, is the effective removal of contaminant gases. This includes radon which produce unwanted background events, but also common pollutants such as water, oxygen and nitrogen, which can capture ionisation electrons, resulting in loss of detector gas gain over time. We present here a novel molecular sieve (MS) based gas recycling system for the simultaneous removal of both radon and common pollutants from SF6. The apparatus has the additional benefit of minimising gas required in experiments and utilises a Vacuum Swing Adsorption (VSA) technique for continuous, long-term operation. The gas system's capabilities were tested with a 100 L low-pressure SF6 Time Projection Chamber (TPC) detector. For the first time, we present a newly developed low-radioactive MS type 5 Å. This material was found to emanate radon at 98% less per radon captured compared to commercial counterparts, the lowest known MS emanation at the time of writing. Consequently, the radon activity in the TPC detector was reduced, with an upper limit of less than 7.2 mBq at a 95% confidence level (C.L.). Incorporation of MS types 3 Å and 4 Å to absorb common pollutants was found successfully to mitigate against gain deterioration while recycling the target gas.

Assessment of Radioactivity of Some Samples of Healthy Drinking Water and Liquefied Water in Some Areas of the Capital Baghdad Using HPGE Detector System

In this work, a total of 20 water samples were collected from various locations inside the city of Baghdad. Each sample consists of a volume of 1 litre of water which had been obtained from multiple geographical locations. There were twelve water samples representing pure tap water sourced from various locations additionally, eight samples of mineral water were obtained from several marketplaces in Baghdad. A total of six radionuclides were detected in the water samples using the HPGe detector. These radionuclides were identified as Bi 214, Ra 226, TI 208, Bi 212, Pb 212, and K 40. Among these, Bi 214 and Ra 226 are part of the U-238 series, while TI 208, Bi 212, and Pb 212 belong to the Th 232 series. Additionally, K 40 is a naturally occurring radionuclide. The observed minimum value for the average specific activity of radionuclides in pure tap water samples was Al-Yarmouk Pure tap water. However, the sample Hay Al-qahira pure tap water exhibited the highest recorded specific activity values of radionuclides in pure tap water. The observed minimum value for the average specific activity of radionuclides in mineral water samples was Bardaa mineral water. However, the sample (W15) exhibited the highest recorded values for the specific activity of radionuclides in mineral water. The measured external dosage of natural radioactivity in water samples from Baghdad was found to be relatively low, at 0.366 mSv.y-1. This value falls below the recommended limit the United Nations Scientific Committee set on the Effects of Atomic Radiation (UNSCEAR), 1 mSv.y-1.

Design of Environmental Detector System (EDS) application through industrial and vocational collaboration

Politeknik Negeri Medan (POLMED) as a vocational institution and PT Berkat Solusi Teknik as a partner have the same responsibility to solve problems that exist in society. Problems in society related to pollution from industrial waste. This is due to many complaints from the public, NGO, and other organizations regarding environmental damage due to production and economic activities. Based on the problems above, this study is needed to detect and monitor environmental parameters such as CO2 level, lighting, noise, temperature, humidity, and measurement data that can be accessed and displayed in real-time via the internet. The implementation method is strengthening work organization, making EDS prototype brackets, design of an environmental detector system (EDS) prototype, prototype testing, MBKM internship, international conferences, monitoring and coordinating with partner, internal monitoring, and evaluation I and II, and preparation of final report. We innovate in the selection of components and the technology used. This collaboration is a symbiotic mutualism between POLMED and industry. The results are in average values, namely the density of dust particles is 1.418 µg/m3. Temperature 36.60oC. Humidity 53.82%RH. Light intensity 10,615 lux. These values above are still normal condition, and there is no pollution in campus. Especially for temperature and humidity. This condition above on the threshold.

Survey of Natural Radioactivity of Soil and Rock Samples and their Radiological Implications to Human Health in Ugwuele Quarry Mining Site, Uturu, Abia State, Nigeria

This work measured the activity concentration of natural radionuclides 238U, 232Th and 40K in soil and rock samples collected in Ugwuele mining site and it surrounding communities in Uturu, Abia state, Nigeria. Fifteen different samples collected were analyzed. A NaI (Tl) spectrometry detector system was adopted for this research. The results obtained were used to estimate the radiological implication of the quarry mining in the study area. The average gamma activity obtained for 238U, 232Th and 40K in soil were 53.39 ± 3.08, 76.79 ± 9.66 and 1061.02 ± 15.72 Bq/kg respectively and 55.09 ± 5.71, 90.96 ± 16.10 and 1281.78 ± 6.38 Bq/kg respectively for rock samples. The calculated radiological hazard indices from Absorbed dose rate, Annual effective dose, Radium equivalent, internal hazard index and External hazard index gave 120.25 nGy/h, 0.147 msv/yr, 247.5 Bq/kg, 0.833 and 0.687 respectively. The average dose rate was higher than the recommended limit. Therefore, soil and rock materials found in Ugwuele could increase the radiological hazards on miners and residents of the area due to long time cumulative exposure.

Smart LPG Leakage Monitoring and Control System Using Gas Sensor (MQ-X), AWS IoT, and ESP Module

Purpose: Nowadays, LPG is used in almost every kitchen for cooking. It has lots of advantages over other cooking fuels. We need to maintain compliance when we cook using it. The gas flows through a couple of equipment like a regulator, pipe, knob, and burner. If equipment got damaged or the regulator was kept on after cooking, it emitted the gas. This event is dangerous and destructive. So, once it is emitted, it should be detected immediately, and emissions should be stopped. There are several sensors available to detect the gas. We demonstrate using this sensor to build an intelligent gas monitoring system here. When the gas is detected, we regulate the valve and stop the gas emission using the control mechanism. Design/Methodology: There are two approaches to designing the LPG detector for the researcher. One approach is to create from scratch, using the raw sensor, creating the schematics, and then building the PCB. After that, the components are assembled and tested in a natural environment. Finally, it would be best if we pack within a suitable enclosure. It takes time to make a testable product. The other approach is for rapid prototyping. Here, we followed the fast procedure. To minimize the hardware overhead, we purchased one complete gas detector system, which is available in the market, and then we added our intelligence to it. Findings/Result: We tested the prototype in a natural environment. The result was found as expected and very promising. We inject the LPG into the sensor. After a couple of seconds, the built-in relay got energized. The ESP module sends the message to the predefined mobile number. Originality/Value: Several projects have already been done to detect LPG leakage. Most of the project monitors gas; it produces an alarm if leakage happens. But here we added a couple of facilities. One is when gas leakage is detected instantly; it triggers the close of the regulator valve. The second one is the incident time, which will be uploaded to the cloud, not local device memory. It will help for audit purposes. Once a leak is detected, we can send the message to the What’s App. These features provide value to the user. Type of Paper: Experimental-based Research.

An 8-channel low power ASIC for Helium-3 tube position sensitive neutron detectors

The majority of neutron spectrometers designated for use in the China Spallation Neutron Source (CSNS) rely on position-sensitive detectors that utilize Helium-3 tube detector technology. To minimize the impact of air on neutron scattering experiments, detectors must be located inside a vacuum chamber. For these types of spectrometers, it is also advisable to house the readout electronics within the vacuum chamber to limit the number of feed-through cables required and enhance the signal-to-noise ratio. This paper proposes an application-specific integrated circuit (ASIC) dedicated to position-sensitive Helium-3 tube detectors, including front-end amplification, shaping module and readout driving buffer. The 8-channel chip, named HEROCV1 (HElium-3 ReadOut Circuits), achieves an input dynamic range from 10 fC to 1 pC, with a counting rate of up to 100 kHz. The equivalent noise charge (ENC) measurement is 1297e-@15pF, and the power consumption is less than 9.9 mW per channel. The ASIC has replaced the traditional front-end circuit based on discrete components, fundamentally addressing the issue of front-end power consumption and enabling the entire detector system to operate in a vacuum environment.

Operational experience with Adaptive Gain Integrating Pixel Detectors at European XFEL

The European X-ray Free Electron Laser (European XFEL) is a cutting-edge user facility that generates per second up to 27,000 ultra-short, spatially coherent X-ray pulses within an energy range of 0.26 to more than 20 keV. Specialized instrumentation, including various 2D X-ray detectors capable of handling the unique time structure of the beam, is required. The one-megapixel AGIPD (AGIPD1M) detectors, developed for the European XFEL by the AGIPD Consortium, are the primary detectors used for user experiments at the SPB/SFX and MID instruments. The first AGIPD1M detector was installed at SPB/SFX when the facility began operation in 2017, and the second one was installed at MID in November 2018. The AGIPD detector systems require a dedicated infrastructure, well-defined safety systems, and high-level control procedures to ensure stable and safe operation. As of now, the AGIPD1M detectors installed at the SPB/SFX and MID experimental end stations are fully integrated into the European XFEL environment, including mechanical integration, vacuum, power, control, data acquisition, and data processing systems. Specific high-level procedures allow facilitated detector control, and dedicated interlock systems based on Programmable Logic Controllers ensure detector safety in case of power, vacuum, or cooling failure. The first 6 years of operation have clearly demonstrated that the AGIPD1M detectors provide high-quality scientific results. The collected data, along with additional dedicated studies, have also enabled the identification and quantification of issues related to detector performance, ensuring stable operation. Characterization and calibration of detectors are among the most critical and challenging aspects of operation due to their complex nature. A methodology has been developed to enable detector characterization and data correction, both in near real-time (online) and offline mode. The calibration process optimizes detector performance and ensures the highest quality of experimental results. Overall, the experience gained from integrating and operating the AGIPD detectors at the European XFEL, along with the developed methodology for detector characterization and calibration, provides valuable insights for the development of next-generation detectors for Free Electron Laser X-ray sources.

Optimisation of the bone single photon emission computed tomography in oncology patients

Introduction: The introduction of hybrid imaging systems such as Single Photon Emission Computed Tomography/Computed Tomography (SPECT/CT) has completely changed the scanning procedure of conventional diagnostic nuclear medicine protocols. Modern bone scintigraphy protocols include SUV quanitification and Whole Body (WB) SPECT/CT scanning modality. The major limitation of these new technologies is relatively long scanning time. New detector systems with modern reconstruction softwares have been developed for fast scanning SPECT protocolos. These new technologies can produce images of reduced acquisition with same quality as full scanning acquisition. As a result new studies suggest that planar WB scintigraphy should be replaced with WB SPECT/CT. Methods: One hundred oncology patients performed SPECT/CT as a part as their clinical follow-up. Three different scanning and three reconstruction protocols have been evaluated. Two nuclear medicine physicians evaluated with Likert scale image sharpness, lesion visibility, and lesion background detectability. The overall image quality was determined as the sum of these three parameters. Results: In terms of scan duration reduction on image quality, Volumetrix Evolution for Bone performed during ultra-fast SPECT acquisition achieved the highest score, which is superior compared to the standard SPECT acquisition protocol. The overall image quality was the best with the Volumetrix MI Evolution for Bone protocol for ultra-fast acquisition Conclusion: The Evolution for Bone protocol for ultra-fast acquisition showed best results compared to other protocols. The adoption of new acquisition SPECT protocol may offer more comfortable exams, resulting in higher patient satisfaction. The implementation of this new protocol can lead to an improvement in SPECT sensitivity, primarily due to the reduction of SPECT motion artifacts.

The generation of noise-like pulses and various solitons with CuO nanorods as a broadband saturable absorber

Copper oxide (CuO) nanorods exhibit unique photoelectric properties due to their high surface area, excellent volume effect, and quantum confinement effect, which have also attracted wide attention in the application of ultrafast photonics. In the present work, CuO nanorods were successfully prepared by a simple synthesis strategy and characterized in detail. The density of electron states (DOS) and the band structure of CuO were predicted by the first principles simulation method. The nonlinear optical response parameters of CuO nanorods were determined by a twin-balanced detector system. Based on the excellent nonlinear optical response, CuO nanorods were exploited as saturable absorbers (SAs) to achieve the noise-like pulse (NLP) mode-locking operation in the erbium (Er)-doped fiber laser (EDFL) at 1552 nm and ytterbium (Yb)-doped fiber laser (YDFL) at 1038.5 nm. Additionally, by shortening the cavity length of EDFL and finely optimizing the intracavity polarization, various solitons, and dual-wavelength solitons were also observed. Moreover, the dynamic evolution of spectra and pulses in YDFL was simulated using scalar nonlinear Ginzburg-Laudau equation (GLE). Our work well demonstrated the broadband and high-efficiency saturable absorption in CuO nanorods, paving the path for investigating multi-soliton dynamics and broadband ultrafast photonics.

Submillisecond in situ X-ray diffraction measurement system with changing temperature and pressure using diamond anvil cells at BL10XU/SPring-8.

Recently, there has been a high demand for elucidating kinetics and visualizing reaction processes under extreme dynamic conditions, such as chemical reactions under meteorite impact conditions, structural changes under nonequilibrium conditions, and in situ observations of dynamic changes. To accelerate material science studies and Earth science fields under dynamic conditions, a submillisecond in situ X-ray diffraction measurement system has been developed using a diamond anvil cell to observe reaction processes under rapidly changing pressure and temperature conditions replicating extreme dynamic conditions. The development and measurements were performed at the high-pressure beamline BL10XU/SPring-8 by synchronizing a high-speed hybrid pixel array detector, laser heating and temperature measurement system, and gas-pressure control system that enables remote and rapid pressure changes using the diamond anvil cell. The synchronized system enabled momentary heating and rapid cooling experiments up to 5000 K via laser heating as well as the visualization of structural changes in high-pressure samples under extreme dynamic conditions during high-speed pressure changes.

Pressurized liquid extraction assisted by high-intensity ultrasound to obtain hesperidin from lime waste: Integration of in-line purification and on-line chromatographic analysis

Hesperidin is a phenolic compound usually found in citrus fruits, which is known for its anti-inflammatory and antioxidant properties. This bioactive compound has already been used to formulate medications to treat chronic venous insufficiency. In this work, through a system which allows the in-line coupling of the pressurized liquid extraction (PLE) and high-intensity ultrasound (HIUS) with solid phase extraction (SPE), and analysis by high-performance liquid chromatography with UV–Vis detector (HPLC-UV) in on-line mode, a method was developed to obtain, separate, and quantify hesperidin from the industrial waste of lime. An eco-friendly approach with water and ethanol as extraction solvents was used. Parameters such as temperature (80, 100, and 120 °C) and HIUS power (0, 200, and 400 W) were evaluated regarding hesperidin yield. In this context, the higher hesperidin yield (18.25 ± 1.52 mg/g) was achieved using water at a subcritical state (120 °C and 15 MPa). The adsorbent SepraTM C-18-E isolated hesperidin from the other extracted compounds employing 50% ethanol in the SPE elution. The possibility ofon-lineanalysis coupling a high-performance liquid chromatograph to an ultraviolet detector (HPLC-UV) system was studied and shown to be a feasible approach for developing integrated technologies. Conventional extractions and their antioxidant capacities were evaluated, highlighting the advantages of the HIUS-PLE-SPE extractive method. Furthermore, the on-linechromatographic analysis showed the potential of the HIUS-PLE-SPE- HPLC-UV system to quantify the extracted compounds in real time.

Development of a near-infrared single-photon 3D imaging LiDAR based on 64×64 InGaAs/InP array detector and Risley-prism scanner.

A near-infrared single-photon lidar system, equipped with a 64×64 resolution array and a Risley prism scanner, has been engineered for daytime long-range and high-resolution 3D imaging. The system's detector, leveraging Geiger-mode InGaAs/InP avalanche photodiode technology, attains a single-photon detection efficiency of over 15% at the lidar's 1064 nm wavelength. This efficiency, in tandem with a narrow pulsed laser that boasts a single-pulse energy of 0.5 mJ, facilitates 3D imaging capabilities for distances reaching approximately 6 kilometers. The Risley scanner, composing two counter-rotating wedge prisms, is designed to perform scanning measurements across a 6-degree circular field-of-view. Precision calibration of the scanning angle and the beam's absolute direction was achieved using a precision dual-axis turntable and a collimator, culminating in 3D imaging with an exceptional scanning resolution of 28 arcseconds. Additionally, this work has developed a novel spatial domain local statistical filtering framework, specifically designed to separate daytime background noise photons from the signal photons, enhancing the system's imaging efficacy in varied lighting conditions. This paper showcases the advantages of array-based single-photon lidar image-side scanning technology in simultaneously achieving high resolution, a wide field-of-view, and extended detection range.