Active mobility and light vehicles, such as e-bikes, are gaining increasing attention as sustainable transportation alternatives to internal combustion solutions. In this context, collecting comprehensive data on environmental conditions, vehicle performance, and user interaction is crucial for improving system efficiency and user experience. This paper presents a data acquisition system designed to collect data from multiple sensor platforms. The architecture is optimized to maintain low power consumption and operate within limited computational resources, making it suitable for real-time data acquisition on light vehicles. To achieve this, a data acquisition module was developed using a single-board computer integrated with a custom shield, which also captures data related to the assistance of an e-bike motor through a wireless interface. The paper provides an in-depth discussion of the architecture and software development, along with a detailed overview of the sensors used. A demonstrator was created to verify the system architecture idea and prove the potentialities of the system overall. The demonstrator has been qualified by professional and semi-professional riders in the framework of the Giro-E, a cyclist event which took place in May 2024, on the same roads of the Giro d’Italia. Finally, some preliminary analyses on the data acquired are provided to show the performance of the system, particularly in reconstructing the user behavior, the environmental parameters, and the type of road.