Resuscitation with plasma components has been shown to improve endotheliopathy induced by hemorrhagic shock, but the optimal resuscitation strategy to preserve the endothelial glycocalyx has yet to be defined. The aim of this study was to determine if resuscitation with lactated ringers (LR), whole blood (WB), packed red blood cells (RBC), platelet rich plasma (PRP), platelet poor plasma (PPP), balanced RBC:PRP (1:1), or day 14 RBC (d14) would best minimize endothelial damage following shock. Male C57BL/6 mice were hemorrhaged to a goal mean arterial pressure (MAP) of 25 mm Hg for one hour. Unshocked sham mice served as controls. Mice were then resuscitated with equal volumes of LR, WB, RBC, PRP, PPP, 1:1, or d14 RBC and then sacrificed at 1-, 4-, or 24-hours (n = 5). Serum was analyzed for syndecan-1, ubiquitin C-terminal hydrolase L1 (UCHL-1), and cytokine concentrations. Lungs underwent syndecan-1 immunostaining and lung injury scores were calculated after H&E stains. Proteolytic cleavage of the endothelial glycocalyx was assessed by serum matrix metalloprotease 9 (MMP-9) levels. Serum syndecan-1 and UCHL-1 levels were significantly increased following resuscitation with d14 RBC compared to other groups. Early elevation in lung syndecan-1 staining was noted in LR treated mice while d14 mice showed decreased staining compared to sham mice following shock. Lung injury scores were significantly elevated 4 hours after resuscitation with LR and d14 RBC compared to WB. Serum MMP-9 levels were significantly increased at 1 and 4-hours in d14 mice compared to sham mice. Systemic inflammation was increased in animals receiving LR, 1:1, or d14 RBC. Resuscitation with WB following hemorrhagic shock reduces endothelial syndecan-1 shedding and mitigates lung injury. Aged RBC and LR fail to attenuate endothelial injury following hemorrhagic shock. Further research will be necessary to determine the effect of each of these resuscitative fluids in a hemorrhagic shock model with the addition of tissue injury.