Switching Platform Research Articles

Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection

A novel graphene oxide (GO) fluorescence switch-based homogenous system has been developed to solve two problems that are commonly encountered in conventional GO-based biosensors. First, with the assistance of toehold-mediated nonenzymatic amplification (TMNA), the sensitivity of this system greatly surpasses that of previously described GO-based biosensors, which are always limited to the nM range due to the lack of efficient signal amplification. Second, without enzymatic participation in amplification, the unreliability of detection resulting from nonspecific desorption of DNA probes on the GO surface by enzymatic protein can be avoided. Moreover, the interaction mechanism of the double-stranded TMNA products contains several single-stranded toeholds at two ends and GO has also been explored with combinations of atomic force microscopy imaging, zeta potential detection, and fluorescence assays. It has been shown that the hybrids can be anchored to the surface of GO through the end with more unpaired bases, and that the other end, which has weaker interaction with GO, can escape GO adsorption due to the robustness of the central dsDNA structures. We verified this GO fluorescence switch-based detection system by detecting microRNA 21, an overexpressed non-encoding gene in a variety of malignant cells. Rational design of the probes allowed the isothermal nonenzymatic reaction to achieve more than 100-fold amplification efficiency. The detection results showed that our strategy has a detection limit of 10 pM and a detection range of four orders of magnitude.

Correcting loss of a papilla following orthodontic space opening (Atherton patch) through implant supported rehabilitation. A case report

The objective of this case report is to describe a surgical and prosthetic technique to create a lost papilla following orthodontic space opening (Atherton´s patch) through implant supported rehabilitation. A switching platform implant was used to replace a left maxillary canine in a unitary interdental edentulous ridge with Atherton´s patch in the distal area of the upper lateral left incisor. The radiographic study revealed correct level of the interproximal bone of the adjacent teeth. A mucoperiosteal flap with crest incision and sulcular extension to the adjacent teeth was made. Special attention was paid to correct position of the implant and the distance (≥ 1.5 mm) between the platform and the roots of the adjacent teeth. A submerged technique was used. Tissue modeling through provisional crown was performed in order to create an ideal emergence profile with total papilla fill recorded at the Atherton´s patch area. Final screw retained CAD-CAM zirconia structure was place. Final follow up was performed 2 years after provisional crown placement, and total fill of both papilla, including at Atherton´s patch area, was recorded. Key words:Atherton´s patch, papilla, switching platform, implant and orthodontics, esthetic score.

Management System in Routing and Switching Platforms

Routing and switching platforms are widely used in modern communication equipments, network management based on routing and switching platform has a significant meaning and a prospect of application. The article briefly describes the routing and switching platform ZebOS and the Simple Network Management Protocol SNMP, it explains the implementation method and workflow of SNMP network management used in routing and switching platform, and it realizes the configuration and management of routing exchange platform.

Influence of Platform and Abutment Angulation on Peri-Implant Bone. A Three-Dimensional Finite Element Stress Analysis

The aim of this study was to evaluate stress distribution on the peri-implant bone, simulating the influence of Nobel Select implants with straight or angulated abutments on regular and switching platform in the anterior maxilla, by means of 3-dimensional finite element analysis. Four mathematical models of a central incisor supported by external hexagon implant (13 mm × 5 mm) were created varying the platform (R, regular or S, switching) and the abutments (S, straight or A, angulated 15°). The models were created by using Mimics 13 and Solid Works 2010 software programs. The numerical analysis was performed using ANSYS Workbench 10.0. Oblique forces (100 N) were applied to the palatine surface of the central incisor. The bone/implant interface was considered perfectly integrated. Maximum (σmax) and minimum (σmin) principal stress values were obtained. For the cortical bone the highest stress values (σmax) were observed in the RA (regular platform and angulated abutment, 51 MPa), followed by SA (platform switching and angulated abutment, 44.8 MPa), RS (regular platform and straight abutment, 38.6 MPa) and SS (platform switching and straight abutment, 36.5 MPa). For the trabecular bone, the highest stress values (σmax) were observed in the RA (6.55 MPa), followed by RS (5.88 MPa), SA (5.60 MPa), and SS (4.82 MPa). The regular platform generated higher stress in the cervical periimplant region on the cortical and trabecular bone than the platform switching, irrespective of the abutment used (straight or angulated).

OpenFlow Implementation on NetMagic Platform

Stanford University proposed a new network switch model named OpenFlow for the research of network innovation. OpenFlow enables researchers to control the behavior of the network by managing flow table. NetMagic is an open network switching platform for the innovative research of next generation Internet architecture. This paper introduces OpenFlow and NetMagic briefly, and implements a customized and scalable OpenFlow model system on the NetMagic platform. Finally, this paper shows the features and advantages of OpenFlow with an experiment.

Influence of implant neck design and implant–abutment connection type on peri‐implant health. Radiological study

To carry out a comparative study of two implants with different neck features and prostheses platform connection (machined with external connection and rough-surfaced with switching platform) upon peri-implant marginal bone loss, before and after functional loading. A randomized, prospective radiological study was made. Eighteen totally edentulous patients were selected. Subjects were divided into two groups according to the type of implant neck used: (a) Osseous(®), with machined surface, without microthreads, external connection, and without platform switching; and (b) Inhex(®), with treated surface, microthreads, internal connection, and platform switching. Mesial and distal marginal bone loss was measured. Implant success was assessed according to the criteria of Buser. Control timepoints were as follows: (a) at implant placement; (b) at prosthesis placement; (c) 6 months after loading; (d) 12 months after loading. Fifteen patients that received 120 dental implants were included: 47% Osseous(®) group and 53% Inhex(®) group. Global mean marginal bone loss with Osseous(®) was 0.27 ± 0.43 mm and 0.38 ± 0.51 mm as determined 6 and 12 months after prosthetic loading, respectively, whereas in the case of Inhex(®) was 0.07 ± 0.13 and 0.12 ± 0.17 mm. These differences were statistically significant (P = 0.047). Difference between Osseous(®) and Inhex(®) in maxilla (P = 0.272) and mandibular (P = 0.462) bone loss were not statistically significant. Bone loss after 6 and 12 months proved statistically significant between two groups, with comparatively greater loss in the case of Osseous(®) implants vs. Inhex(®) implants. Regardless the heterogeneity of the two groups (neck shape, microthreads, surface texture), the implant-abutment connection appears to be a significant factor on peri-implant crestal bone levels. Anyway, in both groups, the values obtained were within normal ranges described in the literature.

Influence of microthreads and platform switching on stress distribution in bone using angled abutments

PurposeTo evaluate the stress distribution in peri-implant bone by simulating the effect of an implant with microthreads and platform switching on angled abutments through tridimensional finite element analysis. The postulated hypothesis was that the presence of microthreads and platform switching would reduce the stress concentration in the cortical bone. MethodsFour mathematical models of a central incisor supported by an implant (5.0mm×13mm) were created in which the type of thread surface in the neck portion (microthreaded or smooth) and the diameter of the angled abutment connection (5.0 and 4.1mm) were varied. These models included the RM (regular platform and microthreads), the RS (regular platform and smooth neck surface), the SM (platform switching and microthreads), and the SS (platform switching and smooth neck). The analysis was performed using ANSYS Workbench 10.0 (Swanson Analysis System). An oblique load (100N) was applied to the palatine surface of the central incisor. The bone/implant interface was considered to be perfectly integrated. Values for the maximum (σmax) and minimum (σmin) principal stress, the equivalent von Mises stress (σvM), and the maximum principal elastic strain (ɛmax) for cortical and trabecular bone were obtained. ResultsFor the cortical bone, the highest σmax (MPa) were observed for the RM (55.1), the RS (51.0), the SM (49.5), and the SS (44.8) models. The highest σvM (MPa) were found for the RM (45.4), the SM (42.1), the RS (38.7), and the SS models (37). The highest values for σmin were found for the RM, SM, RS and SS models. For the trabecular bone, the highest σmax values (MPa) were observed in the RS model (6.55), followed by the RM (6.37), SS (5.6), and SM (5.2) models. ConclusionThe hypothesis that the presence of microthreads and a switching platform would reduce the stress concentration in the cortical bone was partially rejected, mainly because the microthreads increased the stress concentration in cortical bone. Only platform switching reduced the stress in cortical bone.

Short Implant to Support Maxillary Restorations

The aim of this study was to evaluate stress distribution on peri-implant bone simulating the influence of implants with different lengths on regular and switching platforms in the anterior maxilla by means of three-dimensional finite element analysis. Four mathematical models of a central incisor supported by an external hexagon implant (diameter, 5.0 mm) were created, varying the length (15.0 mm for long implants [L] and 7.0 mm for short implants [S]) and the diameter of the abutment platform (5.0 mm for regular models [R] and 4.1 mm for switching models [S]). The models were created using the Mimics 11.11 (Materialise) and SolidWorks 2010 (Inovart) software. Numerical analysis was performed using ANSYS Workbench 10.0 (Swanson Analysis System). Oblique forces (100 N) were applied to the palatine surface of the central incisor. The bone/implant interface was considered perfectly integrated. Maximum (σ(max)) and minimum (σ(min)) principal stress values were obtained. For the cortical bone, the highest stress values (σ(max)) were observed in the SR (73.7 MPa) followed by LR (65.1 MPa), SS (63.6 MPa), and LS (54.2 MPa). For the trabecular bone, the highest stress values (σ(max)) were observed in the SS (8.87 MPa) followed by the SR (8.32 MPa), LR (7.49 MPa), and LS (7.08 MPa). The influence of switching platform was more evident for the cortical bone in comparison with the trabecular bone for the short and long implants. The long implants showed lower stress values in comparison to the short implants, mainly when the switching platform was used.

Regular and Platform Switching: Bone Stress Analysis Varying Implant Type

This study aimed to evaluate stress distribution on peri-implant bone simulating the influence of platform switching in external and internal hexagon implants using three-dimensional finite element analysis. Four mathematical models of a central incisor supported by an implant were created: External Regular model (ER) with 5.0 mm × 11.5 mm external hexagon implant and 5.0 mm abutment (0% abutment shifting), Internal Regular model (IR) with 4.5 mm × 11.5 mm internal hexagon implant and 4.5 mm abutment (0% abutment shifting), External Switching model (ES) with 5.0 mm × 11.5 mm external hexagon implant and 4.1 mm abutment (18% abutment shifting), and Internal Switching model (IS) with 4.5 mm × 11.5 mm internal hexagon implant and 3.8 mm abutment (15% abutment shifting). The models were created by SolidWorks software. The numerical analysis was performed using ANSYS Workbench. Oblique forces (100 N) were applied to the palatal surface of the central incisor. The maximum (σ(max)) and minimum (σ(min)) principal stress, equivalent von Mises stress (σ(vM)), and maximum principal elastic strain (ε(max)) values were evaluated for the cortical and trabecular bone. For cortical bone, the highest stress values (σ(max) and σ(vm) ) (MPa) were observed in IR (87.4 and 82.3), followed by IS (83.3 and 72.4), ER (82 and 65.1), and ES (56.7 and 51.6). For ε(max), IR showed the highest stress (5.46e-003), followed by IS (5.23e-003), ER (5.22e-003), and ES (3.67e-003). For the trabecular bone, the highest stress values (σ(max)) (MPa) were observed in ER (12.5), followed by IS (12), ES (11.9), and IR (4.95). For σ(vM), the highest stress values (MPa) were observed in IS (9.65), followed by ER (9.3), ES (8.61), and IR (5.62). For ε(max) , ER showed the highest stress (5.5e-003), followed by ES (5.43e-003), IS (3.75e-003), and IR (3.15e-003). The influence of platform switching was more evident for cortical bone than for trabecular bone, mainly for the external hexagon implants. In addition, the external hexagon implants showed less stress concentration in the regular and switching platforms in comparison to the internal hexagon implants.

Comparison Between Immediate and Delayed Laser-Treated Implants Surface With Switching Platform: A Clinical Retrospective Study

The aim of the present study was to compare immediate (Im) versus delayed (De) placement of laser-treated implants surface with switching platform to confirm the predictability and performance of this type of implant. The implants were placed in pos exodontia and healed sites at the incisor, canine, premolar, and molar regions of the maxilla or the mandible. A protocol was prepared in which patient age, sex, implant length, diameter, and use of bone graft were recorded. The study included 44 GEASS Srl (Udine, Italy) implants with laser surface and morse taper connection, placed in 27 patients (mean age: 56 years; range: 25-80 years).The survival rates were 100% in the Im group and in the De group. The patients were followed for a minimum of 12 months. Implants with laser surface and morse connection presented when placed in fresh sockets showed similar results to implants placed in mature bone after 12 months of follow-up.

A hybrid optical packet and wavelength selective switching platform for high-performance data center networks

We propose and experimentally demonstrate for the first time a hybrid optical packet and wavelength selective switching platform for high-performance data center networks. This architecture based on cascaded silicon microrings and semiconductor optical amplifiers (SOAs) supports wavelength reconfigurable packet and circuit switching, and is highly scalable, energy efficient and potentially integratable. By combining the wavelength-selective behavior of the microring and the broadband behavior of the SOA switch, we are able to achieve fast switching transitions, high extinction ratios, and low driving voltages, which are all requirements for future optical high-performance data center networks. Routing correctness and error-free operation (<10(-12)) are verified for both 10-Gb/s and 40-Gb/s packets and streaming data with format transparency.

Using NetMagic to observe fine-grained per-flow latency measurements

We introduce NetMagic to demonstrate the efficacy of RLI architecture RLI for the fine-grained per-flow latency measurements. In this demo, the main function of RLI is implemented in NetMagic, which is the key component of our experimental network comprising several computers and switches. We are going to show how NetMagic can provide rapid implementation and evaluation of RLI architecture that is difficult with commercial switch or router platforms. In the demo, the estimated fine-grained per-flow latency by RLI is monitored and dynamically presented. Further, the true latency with a resolution of 8ns is also provided by NetMagic for the evaluation. The efficacy of RLI architecture can be observed in a real-time fashion by the difference between estimated latencies and true ones.

Immunohistochemical evaluation of pro-inflammatory molecules in the soft tissue surrounding switching platform implants

Introduction . Switching platform implants have been proposed to reduce the peri-implant bone loss by shifting the implant abutment interface internally and away from the bone tissue. Aim of the present study was to characterize the inflammatory infiltrate in the soft tissue surrounding switching and matching platform implants and to evaluate the expression of pro-inflammatory molecules involved in the bone loss. Material and methods. A total of 32 implants with diameters of 3.8mm, 4.3mm, 4.8mm and 5.5mm were all restored with abutment of 3.8mm of diameter, thus resulting the following implant-abutment mismatches: 0mm (n=10) (control group), 0.25mm (n=7) (test group1), 0.5mm (n=8) (test group2), and 0.85mm (n=7) (test group3). Four years after loading, peri-implant soft tissue samples were harvested and processed for immunohistochemical analysis. Total amount of lymphocytes T (LyT) -B (LyB) infiltrated, and expression of IL-17 and RANKL were detected. Results . At the harvesting time all sites were clinically healthy. No significant differences were found between groups (p>0.05) in terms of infiltrated T and B cells amount, IL-17 and RANKL expression. In all samples lymphocytes T and B were mainly localized close to the junctional epithelium and sparsely detected in the surrounding connective tissue. The distribution of IL-17 and RANKL staining resulted strictly correlated to the inflammatory infiltrated. When pooled data were analyzed, amount of lymphocytes T and IL-17 were higher than respectively lymphocytes B and RANKL. Amount of LyT and LyB were highly correlated (Pearson’s r>0.7) and IL-17 was moderately correlated (Pearson’s r>0.4, <0.7) to LyT and LyB. Conclusions . In the prolonged exposure of the abutment at the oral cavity the configuration of the implant abutment interface may do not affect the inflammatory cellular and molecular pattern responsible for bone loss.

Soft tissues around long-term platform switching implant restorations: a histological human evaluation. Preliminary results

Switching platform restorations seems to reduce the peri-implant bone resorption and to preserve the peri-implant soft tissues. The aim of the present human study was to compare histologically the peri-implant soft tissue in switching and traditional platform implants 4 years after restoration. Forty-eight months after implant restoration, 37 peri-implant soft tissue samples from 14 patients were harvested from traditionally restored implants (control group) and from three different platforms mismatching 0.25-0.85 mm (test groups). At the harvesting time, all sites were clinically healthy. Samples were processed to evaluate the inflammatory infiltrate area [inflamed connective tissue (ICT)], the microvascular density (MVD) and the collagen content (AA%). At the analyses, no significant differences were found between groups in terms of ICT, MVD and AA% (p>0.05). In all groups, most samples with a well-preserved junctional epithelium showed a small and localized inflammatory infiltrated associated with not-well-oriented collagen fibres and an increased MVD. Forty-eight months after restoration, switching and traditional platform implants had similar histological peri-implant soft tissue features, despite different bone level changes detected radiographically and published in a previous parent study. The present study seems to confirm platform switching as a safe prosthetic concept leading to better maintenance of peri-implant bone levels. However, further histological studies are required to longitudinally confirm the present data.

Topologies of multiterminal HVDC-VSC transmission for large offshore wind farms

AbstractTopologies of multiterminal HVDC-VSC transmission systems for large offshore wind farms are investigated. System requirements for multiterminal HVDC are described, particularly the maximum power loss allowed in the event of a fault. Alternative control schemes and HVDC circuit topologies are reviewed, including the need for HVDC circuit breakers. Various topologies are analyzed and compared according to a number of criteria: number and capacity of HVDC circuits, number of HVDC circuit breakers, maximum power loss, flexibility, redundancy, lines utilization, need for offshore switching platforms and fast communications.

Immediate loading of single tooth: Case report and review of the literature

Aim This was to report a case of immediate loading of a single tooth implant analyzing aesthetic and functional outcomes; a review of the recent literature on the outcomes of immediately loaded single tooth implants is presented, reporting survival rates and providing a classification about success rates of each implant type, in order to define remaining questions requring further investigation in future researches. Materials and methods A case of single tooth replacement and immediate loading with switching platform technique is reported. Moreover 78 scientific texts were selected with the key words “immediate loading single tooth”. Of these only 45 articles specifically analyzed single tooth immediately loaded dental implant; 16 of 45 were available in full text and were therefore investigated. Results The immediate loading of dental implants is well documented in the literature. Several clinical trials show that overall implant survival and success rates are influenced by parameters such as implant design, surface characteristic, bone properties and bone augmentation, implant area placement (upper or lower jaw) and postextraction conditions. However, in single tooth replacement with immediate loading aesthetics (especially in the front area) and functionality (posterior area of the jaw) must be considered. Conclusion This case report and the literature review demonstrated that scientific documentation of implant survival influenced by timing of loading is rather debated. Most of the reported outcome analyses primarily focus on implant survival and follow up. Future researches should analyze how the immediate loading can influence implant success.

Platform Switching and Bone Platform Switching

Bone platform switching involves an inward bone ring in the coronal part of the implant that is in continuity with the alveolar bone crest. Bone platform switching is obtained by using a dental fixture with a reverse conical neck. A retrospective study was performed to evaluate the effectiveness of conventional vs reverse conical neck implants. In the period between May 2004 and November 2007, 86 patients (55 females and 31 males; median age, 53 years) were operated and 234 implants were inserted: 40 and 194 were conventional vs reverse conical neck implants, respectively. Kaplan-Meier algorithm and Cox regression were used to detect those variables associated with the clinical outcome. No differences in survival and success rates were detected between conventional vs reverse conical neck implants alone or in combination with any of the studied variables. Although bone platform switching leads to several advantages, no statistical difference in alveolar crest resorption is detected in comparison with reverse conical neck implants. We suppose that the proximity of the implant abutment junction to the alveolar crestal bone gives no protection against the microflora contained in the micrograph. Additional studies on larger series and a combination of platform switching and bone platform switching could lead to improved clinical outcomes.

Double-blind randomized controlled trial study on post-extraction immediately restored implants using the switching platform concept: soft tissue response. Preliminary report

Double-blind randomized controlled trial study on post-extraction immediately restored implants using the switching platform concept: soft tissue response. Preliminary report

Double‐blind randomized controlled trial study on post‐extraction immediately restored implants using the switching platform concept: soft tissue response. Preliminary report

To evaluate the soft tissue response to immediately placed implants using the platform switching concept. In 22 patients, 22 implants of 5.5 mm platform diameter were placed immediately into fresh extraction sockets in maxillae without compromised bone tissue. Eventual post-extraction bone defects were filled using bovine bone matrix mixed with collagen. Immediately after insertion, implants were randomly divided: 11 implants were connected with a 3.8 mm diameter abutment (test group) and 11 with a 5.5 mm diameter abutment (control group). A provisional crown was adapted and adjusted for non-functional immediate positioning. Two months later, definitive prosthetic rehabilitation was performed. Periodontal parameter, buccal peri-implant mucosal changes (REC), mesial and distal papilla height (PH) and vertical height of jumping distance (VHG) were measured at the time of implant placement, of definitive prosthesis insertion and every 6 months thereafter. The mean follow-up was 25 months. All implants were clinically osseointegrated. The test group showed a +0.18 mm REC gain. PH gain was +0.045 mm on average. The mean values were statistically significant (P< or =0.005) compared with the control group (PH=-0.88 mm; REC=-0.45 mm). No difference between the two groups in periodontal parameters was found. The mean value of bone filling was 7.51 mm in the test group (97.4% of VHG) and 8.57 mm in the control group (95.2% of VHG). No statistically significant difference was found between the two groups. This study suggests that, in a limited time period of 2 years, immediately placed implants with subsequent platform switching can provide peri-implant tissue stability.

Applications of micro hot embossing for optical switch formation

This paper demonstrates applications of micro hot embossing process for fabricating vertical micromirrors and optical switch platforms using polycarbonate as the process material. The effect of process parameters upon the height of mirrors, the thickness of cantilevers and the deflection of switch platforms was investigated. Micromirrors with a dimension of 500 μm (width) ×100 μm (thickness) × 200 μm (height) were embossed at 180 °C under a pressure of 1.75 kg/mm 2. Cantilevers with a thickness around 120 μm were obtained at 200 °C and a pressure of 1.75 kg/mm 2. Considering both the mirror height and the thickness of cantilever, the process temperature and pressure were chosen as ≥185 °C and ≥1.75 kg/mm 2, respectively. Annealing the embossed sample in 140 °C can reduce its deflection to 50–60% of its original value. Measurement using a scanning force microscope illustrated that surface roughness ( R a) of the embossed polymer mirror was around 0.1 μm.