Quality spawns are an important factor to support the success of button mushroom cultivation. The quality of mushroom spawn is inuenced by the nutritional composition of the media. The purpose of this study was to determine the best media for the growth of F0 and F1 button mushroom. The research was carried out in March-May 2021 at the Biology Laboratory, Faculty of Agroindustry, University of Mercu Buana Yogyakarta. The study consisted of 2 series of experiments. This study consisted of 2 series of experiments. The rst experiment was to test the growth quality of F0 champignon on 3 types media, that is potato dextrose agar (PDA),mung been sprout extract media, and white sweet potato media. The second experiment tested the growth quality of F1 button mushroom grown on three types of media, that is corn, barley and sorghum. Both experiment were single factor trials arranged in a completely randomized design (CRD) with three replications, and each replication consisted of 5 units. The results showed that the growth of F0 button mushroom grown on PDA media and mung bean sprout extract was better than white sweet potato extract media. For the growth of F1 button mushroom, the best media was corn, the second was sorghum and the third was barley.