Standardized laboratory testing is normally used for characterization of building materials and components. In real life, especially when assessing buildings in terms of water-damaged building materials, built-in moisture, rising damp or other problems, information on the hygrothermal state of the constructions may be relevant, but is often sparse. This paper presents a study where application of a newly developed non-destructive experimental method is used to measure the relative humidity (RH) at the surface of four types of building materials. Knowing the RH at the surface, a risk assessment of fungal growth can be made. The materials include concrete, brick, aerated concrete, and gypsum, with and without a surface treatment. The method is based on the idea of establishing a water vapor transport towards equilibrium within a small air volume in contact with the surface of interest. In the laboratory the specimens were conditioned at respectively 65 % RH and 90 % RH in a climate chamber, and then transferred to conditions with 50 % RH. The open side of a Petri dish, equipped with a temperature and relative humidity sensor, was attached to the materials using reusable adhesive. The development of the relative humidity over time within the petri dish was monitored and parameterized mathematically, solving an exponential rate equation. The parameterization and certain sets of assumptions allow among other things a calculation of time to equilibrium which makes it possible to assess the reproducibility and comparison in between materials. As the materials were tested with and without surface treatment, it was possible to estimate the effect of a typical surface treatment on the moisture transport. The measurements compared well to simulations made by a model, though the model predicts slightly faster achievement of equilibrium. Despite the limited number of cases in the current study, the presented results are promising with regards to enable rough estimates of RH at the surface of building materials outside the laboratory with a very simple, inexpensive, and non-destructive method.