Abstract

The article presents the classification and specificity of effective methods of protection from bio-damage. Settling on the surface of building materials and structures, microorganisms, along with the destructive effect, worsen the environmental situation in buildings and structures.Studying the works of Russian and foreign authors in the field of biotechnology made it possible to generalize the basic methods of combating biological damage to KSM (carbamide resin), which are divided into physical, biological and chemical.One of the most effective and long — lasting ways to protect building materials and structures from damage by microorganisms is the use of biocidal compounds. The latter are introduced into the composition of the material during its manufacture or by the method of impregnation. Fungicides also inhibit the development of microorganisms by reducing the intake of nutrients from the environment to the cells of the fungus.In world practice, promising biocidal compounds are considered to be preparations based on guanidine, which are effectively used in medicine, the textile industry, in chemical technologies for the synthesis of surfactants, etc. In this article, the inhibitory effect of the preparation “Teflex” (base - polyethylene guanidine) on the activity of exoxidoreductases of fungi destructors of construction composites (Aspergillus fumigatus, Stemphylium verruculosum, Trichoderma koningii and Paecilomyces carneu)s at the maximum of enzymatic activity was evaluated. Biocides were introduced into the fungi culture medium on day 3 (in vivo).It was found that “Teflex” fungicides inhibit phenolosidotoses, cotoloses and peroxidoses: phenol oxidases of the fungus Aspergillus fumigatus - 1.4 times, Paecilomyces carneus - 1.6 times, Trichoderma koningii - 2.0 times, Stemphylium verruculosum - 4.2 times (Figure 7.1 A); catalases of the fungus Paecilomyces carneus - 1.8 times, Trichoderma koningii - 2.2 times, Stemphylium verruculosum - 9.3 times, Aspergillus fumigatus - 23.9 times (Figure 7.1 b); peroxidases of the fungus Aspergillus fumigatus - 3.4 times, Trichoderma koningii-4.6 times, Paecilomyces carneus-9.9 times, Stemphylium verruculosum - 43.7 times. The fungicidal effect of the preparation “Teflex” may be associated with its destabilizing effect on the native structure of enzymes. It leads to a violation of the tertiary structure of the enzyme, causes protein denaturation and disorganization of the cell membrane.

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