Lake surface water temperature (LSWT) is an important physical attribute that affects the ecological environment of lakes. It plays an important role in the ecological process of the water environment and directly affects human production and life, the ecological environment system of the water area, and the diversity of organisms. In order to adjust and improve the ecological conditions of the watershed, it is necessary to obtain a large amount of effective lake surface water temperature data, and use this as a basis to analyze the temporal and spatial change process of the LSWT. This study uses MODIS (MOD11A2) surface temperature product data to obtain the LSWT of Dianchi Lake, and then analyzes the temporal and spatial variation characteristics of the LSWT of Dianchi Lake on different time scales (year, season, month) during the day and night. The research results show that the annual, seasonal and monthly average values of the LSWT during the day and night are all increasing from 2001 to 2017. The average annual temperature rise rate of Dianchi LSWT during the day and night is 0.09°Cyr−1 and 0.05°Cyr−1, respectively. The spatial analysis results show that the LSWT increase area of Dianchi Lake is expanding.