For a long time, the main problem with water intended for human consumption has been health, and this problem stems from the existence of micro-organisms (bacteria, viruses, protozoa, parasites) that transmit many dangerous infections to humans. Vibrio cholerae is one of these micro-organisms. Cholera is a severe acute diarrhoeal disease caused by Vibrio cholerae, a Gram-negative bacterium that colonises warm, saline and alkaline surface waters, often in association with phytoplankton or zooplankton. The aim of this study was to identify bacteria of the Vibrio genus in the waters of the Télimélé and Boké prefectures. The study was carried out from the beginning of May to the end of September 2021. The samples were analysed in the bacteriology laboratory of the Institut de Recherche en Biologie Appliquée de Guinée (IRBAG) in Kindia, using conventional identification methods. The results of the analysis proved the existence of the bacterial strain in the water analysed. During the analyses, we encountered two types of Vibrio: parahaemolyticus with 1.28% in Télimélé and alginolyticus with 2.56% in Boké. These germs were found in wells and lakes, but Vibrio spp was not found. In addition to bacteria of the species Vibrio parahaemolyticus and Vibrio alginolyticus, we encountered two (2) other types of microorganisms: Enterococcus faecalis observed in ponds and streams and Salmonella spp. found in taps, boreholes and wells.