A facile procedure for binding silver nanoparticles on polyester fabrics was reported. In this paper, the multifunctional polymer films were firstly formed through simple dip-coating of polyester fabrics in an aqueous solution of dopamine. Then the silver nanoparticles were in situ generated on the surface of dopamine-modified polyester fabrics in an aqueous solution of silver nitrate under room temperature. X-ray diffraction, scanning electron microscopy and inductively coupled plasma optical emission spectrometry were used to characterize the structure and the content of silver nanoparticles. The antibacterial efficiencies of treated samples were quantitatively estimated. The results indicated that the dopa-polyester fabrics loaded with silver nanoparticles showed durable antibacterial activity.