Surface Molecular Imprinting Technique Research Articles

Analysis of Trace Enrofloxacin in Environmental Waters by a Surface Molecular Imprinting Technique.

Surface-imprinted polymers (ZIF-67@MIPs) supported by ZIF-67 were prepared by precipitation polymerization using enrofloxacin (ENR) as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. ZIF-67@MIPs were characterized by Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy, and particle size distribution. The adsorption performance of the polymer was studied. The adsorption equilibrium was reached within 30 min. The maximum adsorption was 9.02 μg·mg-1. The imprinting factor was 2.58. The polymer was then used as a sorbent of a solid-phase extraction column for the separation and purification of ENR in real water samples. The extraction conditions were optimized. The method was established by high-performance liquid chromatography, and the linearity was verified by UPLC-MSMS. The correlation coefficient and limits of detection and quantification were 0.9999, 0.23 ng·mL-1, and 0.76 ng·mL-1, respectively. The recoveries were in the range of 83.79-100.68%; the relative standard deviation was 4.46-7.35%. The above data indicated that the method could be used for the separation and enrichment of ENR in real samples.

Reusable magnetic molecular imprinted polymers based on magnetic graphene oxide for selective identification and detection of eugenol in environmental water samples.

In this study, a reliable method for determining eugenol content in environmental water samples was established by combining magnetic solid-phase extraction with high-performance liquid chromatography. Magnetic molecular imprinted polymers MGO@MIPs were prepared through surface molecular imprinting technique with eugenol as the template molecule. The material displayed good superparamagnetic properties and magnetic responsiveness in favor of rapid separation. The adsorption properties of MGO@MIPs for eugenol were evaluated through adsorption kinetics and selectivity experiments. MGO@MIPs were found to have favorable reusability and obvious selectivity for eugenol. In addition, adsorption and elution conditions were investigated. Under optimal conditions, a linear relationship was obtained between the concentration of eugenol and its peak area in the range of 0.02-5mg/L (R2 = 0.9998) and the limit of detection was 4.0 × 10-6mg/mL. The performance of the established method was assessed with the average recovery of 96.59-102.20% and the relative standard deviation (RSD) below 3.5%. The application of this method provides a new perspective for the separation, enrichment and detection of eugenol in water environment.

Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas.

Fluorescence intensity and selective recognition ability are crucial factors in determining the analytical techniques for fluorescent probes. In this study, a core-shell fluorescent material, composed of silver nanoparticles@nitrogen-doped graphene quantum dots (Ag NPs@N/GQDs), was synthesised using mango leaves as the raw material through a thermal cracking method, resulting in strong fluorescence luminescence intensity. By employing noradrenaline as a template molecule and using a surface molecular imprinting technique, a molecularly imprinted membrane (MIP) was formed on the surface of the fluorescent material, that was subsequently eluted to obtain a highly specific, fluorescent probe capable of recognising noradrenaline. The probe captured various concentrations of noradrenaline using the MIP, which decreased the fluorescence intensity. Then a method for detecting trace amounts of noradrenaline was established. This method exhibited a linear range from 0.5 -700pM with a detection limit of 0.154pM. The proposed method was implemented in banana samples. Satisfactory recoveries were confirmed at four different concentrations. The method presented a relative standard deviation (RSD) of less than 5.0%.

Selective extraction and quantitative analysis of pyrroloquinoline quinone from food.

Pyrroloquinoline quinone (PQQ) is a bioactive compound that has attracted significant attention due to its potential health benefits. In this study, we developed a new magnetic molecularly imprinted nanoparticle (MMIN) for the selective extraction and determination of PQQ from food samples. The MMIN was synthesized using a surface molecular imprinting technique with PQQ as the template molecule, Fe3O4 nanoparticles as the magnetic core, and methacrylic acid as the functional monomer. The MMIN exhibited high selectivity and affinity towards PQQ, allowing for efficient extraction and preconcentration of PQQ from complex food matrices. The extracted PQQ was then quantified using HPLC-DAD. The developed method showed good linearity (R2 = 0.9985) and low limits of detection (0.03 μg L-1). The accuracy and precision of the method were evaluated by analyzing spiked food samples, with average recoveries close to 89.8%. The MMIN also demonstrated good reusability, with negligible decrease in extraction efficiency after five cycles of use. Overall, the developed MMIN-based method provides a reliable and efficient approach for the analysis of PQQ in food samples.

Magnetic Molecularly Imprinted Polymers for Selective Separation Genistein from the Extract ofFlemingia philippinensisMerr. et Rolfe

AbstractIn this work, a kind of genistein magnetic imprinted polymers (Gen‐MMIPs) was successfully synthesized via surface molecular imprinting technique, in which genistein (Gen) and Fe3O4were used as the template molecule and the carrier, respectively. The obtained Gen‐MMIPs were characterized by TEM, VSM, FT‐IR, and XRD. The adsorption capacity of Gen‐MMIPs for Gen is 33.23 mg/g, with the kinetic adsorption of Gen‐MMIPs reaching equilibrium in 30 min. The imprinting factor (α) is 2.15, which indicated that the Gen‐MMIPs had good selectivity, high binding capacity. The Gen‐MMIPs were evaluated to separate genistein from the crude sample ofFlemingia philippinensisMerr. et Rolfe, which showed that the Gen‐MMIPs can be applied to separate genistein from complicated samples of natural products. These results indicated that the Gen‐MMIPs have great potential for efficient separation and enrichment of genistein in complicated samples.

Selective degradation of ceftriaxone sodium by surface molecularly imprinted BiOCl/Bi3NbO7 heterojunction photocatalyst

By using the surface molecular imprinting technique, BiOCl/Bi3NbO7 was used as the substrate photocatalyst and ceftriaxone sodium (CTRX) was used as the template. A molecularly imprinted photocatalyst with specific recognition and photocatalytic synergy for CTRX was synthesized. Due to the excellent photoelectric properties and the successful formation of CTRX-imprinted cavities on the imprinted layer surface. The characterization results showed that MIP-3 has the advantages of uniform particle size and high separation efficiency of photogenerated carriers. The molecularly imprinted photocatalyst MIP-3 showed remarkable synergy and selectivity in both adsorption-photocatalysis of CTRX. Further experimental results showed that MIP-3 exhibited high selective adsorption performance for both high and low concentrations of CTRX in the 30 min static adsorption experiment. About 92 % of CTRX was degraded in the 100 min light experiment, which was a large improvement compared with BiOCl/Bi3NbO7. The selectivity of MIP-BNO was evaluated by comparing the degradation efficiency of MIP-3 on CTRX and chloramphenicol (CIP). Compared to non-imprinted photocatalyst NIP-BNO, MIP-3 has a higher selectivity coefficient of 2.58 and a faster degradation kinetic rate of 0.0169 min−1. The stability experiments showed that MIP-3 has good recoverability and stability during photocatalytic degradation. The mechanism of selective photocatalytic degradation of CTRX by MIP-3 was investigated by UV–visible spectroscopy, radical capture experiments, and electron spin tests. The possible degradation pathway of CTRX was also analyzed by LC-MS. In conclusion, this study confirms that the combination of surface molecular imprinting and photocatalyst has great potential to provide a promising solution for the treatment of low-level, highly toxic target pollutants in mixed wastewater.

Synthesis of MRGO@ZIF-7-Based Molecular Imprinted Polymer by Surface Polymerization for the Fast and Selective Removal of Phenolic Endocrine-Disrupting Chemicals from Aqueous Environments

In this study, Zn(NO3)2·6H2O was selected as the metal source, and ZIF-7-modified magnetic graphene-based matrix materials (MRGO@ZIF-7) were prepared by in situ growth. ZIF-7 modified magnetic graphene-based molecular imprinting complexes (MRGO@ZIF7-MIP) were successfully synthesized by a surface molecular imprinting technique using bisphenol A (BPA) as the template molecule. The obtained experimental materials were characterized by X-ray diffraction (XRD), Brunner–Emmet–Teller (BET) analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), and X-ray photoelectron spectroscopy (XPS). The proper adsorption and selective recognition ability of the MRGO@ZIF7-MIP were studied by an equilibrium adsorption method. The obtained MRGO@ZIF7-MIP showed significant molecular recognition of bisphenol A (BPA) and good selectivity and reproducibility for BPA in different aqueous environments such as drinking water, river water, and lake water. These properties make this material potentially applicable for the efficient removal of phenolic endocrine disruptors in real water environments.

Dual responsive molecularly imprinted polymers based on UiO-66-DOX for selective targeting tumor cells and controlled drug release

The preparation of synthetic drug delivery systems free of antibody modifications that can effectively recognize tumor sites and control drug delivery is essential for cancer therapy. In this study, UiO-66 metal–organic framework (MOF) modified with amino and phenylboronic acid groups was used as substrate to load the anti-cancer drug doxorubicin (DOX). Then a molecularly imprinted polymer (UiO-66-DOX@MIP) with sialic acid (SA) as the template molecule was further prepared on the surface of the synthesized nanoparticles using surface molecular imprinting technique (MIT). This polymer combines the excellent properties of UiO-66 and molecularly imprinted polymer (MIP). It was simple to prepare, economical and easy to obtain, and had a regular morphology. UiO-66-DOX@MIP containing molecularly imprinted binding sites that match the template molecule SA, could specifically recognize target tumor cells. The imprinted layer prevented drug leakage in normal body fluids while degrading and drug release under tumor microenvironment, with pH and glutathione (GSH) dual-responsive drug release properties. The UiO-66-DOX@MIP showed excellent biocompatibility and great cellular uptake efficiency to cancer cells without influence of interfering substances.

Preparation of sulfamethoxazole molecularly imprinted polymers based on magnetic metal–organic frameworks/graphene oxide composites for the selective extraction of sulfonamides in food samples

Novel sulfamethoxazole molecularly imprinted polymers based on magnetic metal–organic frameworks/graphene oxide composites (MOF/MGO@MIP) were synthesized using the surface molecular imprinting technique. The scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and other characterization methods were used to analyze MOF/MGO@MIP. The adsorption performance of MOF/MGO@MIP was studied via adsorption kinetics, adsorption isotherms and selectivity experiments, and then its adsorption mechanism was investigated using various adsorption models. The adsorption results matched well with a quasi-second-order kinetic model and a Langmuir isotherm model, with the maximum adsorption capacity, equilibrium adsorption time and imprinting factor of 42.65 mg g−1, 120 min and 1.61, respectively. Meanwhile, the MOF/MGO@MIP with excellent recyclability displayed higher adsorption capacity and selectivity than MOF/MGO@NIP. Under the optimal extraction conditions, a method base on MOF/MGO@MIP combined with high-performance liquid chromatography (MOF/MGO@MIP/HPLC-UV) was established to separate and detect trace sulfonamides in honey and milk samples, achieving lower limits of detection (LOD, 0.39–0.47 μg kg−1) with an excellent linear correlation (R2 > 0.9990). The spiked recovery and relative standard deviation (RSD) were 81.38–96.71% and 1.4–6.3%, respectively. Therefore, MOF/MGO@MIP could be regarded as an efficient adsorbent for SAs pretreatment in food samples.

Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products

In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g−1. Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g−1, and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.

Application of a Magnetic Molecularly Imprinted Polymer for the Removal of Sulfanilamide as Major Impurity in Eye Drops (Sulfacetamide)

Magnetic molecularly imprinted polymers (MIPs) have been synthesized for the removal of sulfanilamide as a major impurity in the sulfacetamide eye drops. In this study, the magnetic nanoparticles were modified by tetraethyl orthosilicate and 3-methacryloxypropyl trimethoxysilane before imprinting. The magnetic MIPs were prepared through surface molecular imprinting technique, by using Fe3O4 as a magnetic component, sulfanilamide as template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and 2,2-azobisisobutyronitrile as a radical initiator in acetonitrile/toluene (60:40, v/v) as a porogen. The obtained sorbent was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The selectivity of MIPs for the sulfanilamide impurity absorption was evaluated through adsorption experiments, and the results were compared to the adsorption of impurities by MIPs. Various parameters were optimized including the time, pH, and type of eluent used for the removal of impurities from polymer, concentration of sample, and saturation of polymer. The proposed method was applied to the removal of sulfanilamide from sulfacetamide eye drops. Passing the eye drops through the MIPs led to the quantitative removal of sulfanilamide.

Development of fast resettable gravimetric aromatic gas sensors using quartz crystal microbalance

A surface molecular imprinting technique was applied for fabricating a porous aromatic gas sensing structure on the surface of a quartz crystal chip. A modified layer by layer process between poly (sodium, styrene sulfonate) (PSS) and poly (allylamine hydrochloride) (PAH) in the presence of aromatic compounds was carefully performed to obtain firm and selective films. In the obtained films, two functional areas of the phenyl rings in PSS molecules and hydrophilic static charge interaction sites between PSS and PAH were worked as sensing principles in the gas sensor applications. In the toluene or phenol imprinted film condition, the sensitivities reached to 0.1 ppm and 6.6 ppb (LOD) for the toluene and the phenol gases, and the selectivity value of the phenol to the toluene reached to 67 with our standard. The obtained selectivity and sensitivity were explainable by the nature of the film components and the surface molecular imprint effect. The sub-min level reset time was normally obtained in these measurements.

Fe3O4/graphene molecularly imprinted composite for selective separation of catecholamine neurotransmitters and their analysis in rat brain tissues

In this study, a novel of magnetic molecularly imprinted polymers (Fe3O4/GO/DMIPs) with multi-targets recognizing function were prepared by surface molecular imprinting technique adopting isoprenaline as the dummy-template molecule and graphene oxide (GO) as the carrier. The morphology, structures and magnetic properties of nanosorbents were characterized and assessed in detail and the results indicated that the 3D recognition cavities and matching functional groups with catecholamine neurotransmitters (CNs) were successfully fabricated on Fe3O4/GO surface. Moreover, the kinetic, isothermal and selective adsorption experiments were conducted to further reveal the adsorption behavior of adsorbent toward CNs and the results showed that the Fe3O4/GO/DMIPs possessed high adsorption capacity, rapid binding rate and excellent selectivity for CNs. On this basis, the Fe3O4/GO/DMIPs were further applied as adsorbent of magnetic solid-phase extraction (MSPE) for selective recognition and separation of CNs (dopamine, epinephrine, norepinephrine) followed by UPLC-MS/MS detection. The crucial parameters affecting the extraction efficiency were systematically optimized by Box-Behnken statistical design. Under the optimum conditions, satisfactory linearity (r > 0.99) was obtained with the lower limit of quantification from 0.53 to 1.93 ng mL−1. The accuracy (RE) ranged from −7.6% to 6.4% and the intra- and inter-day precisions were not more than 8.7% and 10.2%, respectively. Hence, the strategy proposed in this study might be used for high selectivity recognition and determination of CNs in complex biological matrices, which would provide a basis and reference for its application in the fields of separation and clinical monitoring.

Dummy-surface molecularly imprinted polymers based on magnetic graphene oxide for selective extraction and quantification of pyrethroids pesticides in fruit juices

A highly binding dummy template molecularly imprinted polymers (Fe3O4-NH2@GO@MIPs) was synthesized used Fe3O4-NH2@GO as carrier by dummy surface molecular imprinting technique (DSMIT) for the first time. Fe3O4-NH2@GO@MIPs were characterized by fourier transform infrared (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric analysis (TGA), brunauer emmett teller (BET) and vibrating specimen magnetometer (VSM). Adsorption and selectivity experiments of Fe3O4-NH2@GO@MIPs and non-imprinted polymers (Fe3O4-NH2@GO@NIPs) verified that the Fe3O4-NH2@GO@MIPs had a better selectivity and adsorption properties for five pyrethroids pesticides. Adsorption test results revealed that the adsorption behaviors of pyrethroids onto Fe3O4-NH2@GO@MIPs followed Langmuir equation adsorption isotherm and pseudo-second-order kinetic models. Fe3O4-NH2@GO@MIPs were used as sorbent in magnetic solid phase extraction (MSPE) to extract five pyrethroids from fruit juice samples, following desorption with acidified ethyl acetate, the pyrethroids were quantified by gas chromatography with electron capture detection (GC-ECD). The developed method MSPE coupled with GC-ECD achieved satisfactory recovery (72.1–106.8%) with RSD less than 10.8%, and the detection limits (LOD) for fenpropathrin (FENP), lambda-cyhalothrin (LC), cyfluthrin (CYF), fenvalerate (FEN) and deltamethrin (DEL) ranged from 0.007 to 0.015 μg/L. Compared to other methods, the procedure MSPE-GC-ECD based on Fe3O4-NH2@GO@MIPs in this work demonstrated to be a more convenient and practical method for the separation, enrichment, and detection of pyrethroids pesticides from fruit juice samples.

Silanized carbon dot-based thermo-sensitive molecularly imprinted fluorescent sensor for bovine hemoglobin detection.

Using the surface molecular imprinting technique, a thermo-sensitive molecularly imprinted fluorescent sensor was constructed for bovine hemoglobin (BHb) detection with the silanized carbon dots (CD@SiO2) as fluorescent signal, N-isopropylacrylamide as monomer sensitive to temperature, and BHb as template. The silanized carbon dots coated by the molecularly imprinted polymer (CD@SiO2@MIP) were characterized by high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and fluorescence spectroscopy. Owing to the combination of the strong fluorescence sensitivity of CDs and the high selectivity of the molecular imprinting shell, the prepared sensor showed good recognition and detection performance to the target protein BHb, with a linear range of 0.31-1.55μM and a detection limit of 1.55μM. Furthermore, the sensor was utilized to detect the content of BHb in real urine with a recovery of 98.6-100.5%. The CD@SiO2@MIP sensors present a high potential for applications in the detection of BHb in biological systems. Graphical abstract.

Magnetic dummy-template molecularly imprinted polymers based on multi-walled carbon nanotubes for simultaneous selective extraction and analysis of phenoxy carboxylic acid herbicides in cereals

A novel of magnetic dummy-template molecularly imprinted polymers (mag-MWCNTs-DMIPs) were prepared by surface molecular imprinting technique. The structure of polymers were characterized and the binding properties were assessed by adsorption experiments. The synthetic mag-MWCNTs-DMIPs exhibit satisfying adsorption capacity, excellent selectivity and fast adsorption rate toward phenoxy carboxylic acid (PCA) herbicides. Afterwards, a reliable analytical method for selective separation and determination of trace PCA herbicides in cereals was established by using magnetic solid-phase extraction (mag-MWCNTs-DMIPs as magnetic adsorbent) and UPLC-MS/MS detection. A series of requisite factors were optimized in detail to achieve expected extraction performance. Under the optimum MSPE parameters, the mean spiked recoveries for analytes in different cereals ranged from 86.7% to 95.2% with intra- and inter-day precision not greater than 8.5% and 10.6%, respectively. At last, the developed method was successfully utilized for determination the four PCA herbicides in actual cereals.

Dummy template surface molecularly imprinted polymers based on silica gel for removing imidacloprid and acetamiprid in tea polyphenols.

Dummy template surface molecularly imprinted polymers based on silica gel were prepared through the surface molecular imprinting technique. Nonpoisonous nicotinamide, which is a structural analogue of imidacloprid and acetamidine, was chosen as the dummy template molecule. The obtained polymers were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The results showed that the polymers exhibited high adsorption capacity and selectivity for imidacloprid and acetamiprid. The maximum adsorption capacities of the polymers toward imidacloprid and acetamiprid were 42.05 and 22.99mg/g, and the adsorption could reach binding equilibrium within 150min. The polymers were successfully applied as column-filling materials to extract imidacloprid and acetamiprid from tea polyphenols with a relatively high removal rate (92.36 and 95.20%). The polymers also showed great stability and reusability during the application. The obtained polymers possessed good application prospects for removing imidacloprid and acetamiprid in tea polyphenol production processes.

A novel bioassay for the monitoring of hydrogen peroxide in human plasma samples based on binding of horseradish peroxidase-conjugated prostate specific antigen to poly (toluidine blue) as imprinted polymer receptor

A low cost, sensitive and selective electrochemical imprinted biosensor for horseradish peroxidase-conjugated prostate-specific antigen (PSA) was designed and prepared based on the combination of Toluidine blue (TB) and self-assembly surface molecular imprinting technique. Poly toluidine blue [P(TB)] provided high surface area for dense loading of HRP-PSA antibody on GCE surface. P(TB), as supporting material, could effectively enhance imprinting efficiency and the electrode conductivity and facilitate electron transfer. The imprinted biosensor was characterized through Field emission scanning electron microscopy (FE-SEM), Energy-dispersive X-ray spectroscopy (EDX) and electrochemical methods. The proposed biosensor indicates very highly electrocatalytical activity for the reduction of hydrogen peroxide (H2O2). Also, engineered biosensor was used for determination of H2O2 by different electrochemical techniques including differential pulse voltammetry, square wave voltammetry and chronoamperometry. Under the optimized conditions, the proposed bio-imprinted polymer exhibit excellent electrocatalytical activity toward the reduction of H2O2 with wide linear range of 0.001 to 40 mM and a low limit of quantification (LLOQ) of 1 μM.

High-efficiency recognition and detection of sulindac in sewage using hydrophilic imprinted resorcinol-formaldehyde resin magnetic nano-spheres as SPE adsorbents combined with HPLC

Hydrophilic molecularly imprinted polymer for effective removal of pollutants from environmental water has attracted much attention due to their high selectivity and matrix compatibility. Herein, resorcinol and formaldehyde have been first proposed as the hydrophilic monomers to form water-compatible molecularly imprinted resin magnetic nanomaterials (Fe3O4-RF-MIPs) by adopting a two-step template immobilization strategy of surface molecular imprinting technique. In the synthesis process, carboxyl-modified Fe3O4 nanoparticles and sulindac were adopted as carrier and template molecule, respectively. The physical and chemical properties, preparation process, and adsorption conditions of Fe3O4-RF-MIPs were investigated in detail. Fe3O4-RF-MIPs exhibit uniform morphology, high crystallinity, outstanding magnetic property, fast binding kinetics, high adsorption capacity, satisfactory selectivity, and excellent reusability. Meanwhile, combining with HPLC, Fe3O4-RF-MIPs as solid phase extraction adsorbents have been successfully applied to specifically enrich and detect sulindac in sewage water. Accordingly, Fe3O4-RF-MIPs with ordered and evenly distributed imprinted sites and excellent dispersibility in water would be potential in the field of water environmental pollution control.

Magnetic molecularly imprinted polymer combined with high performance liquid chromatography for selective extraction and determination of the metabolic content of quercetin in rat plasma

In this study, a novel magnetic solid phase extraction adsorbent was prepared, namely Fe3O4@SiO2 as the carrier, quercetin as the template molecule, acrylamide (AM) as the functional monomer, ethylene glycol dimethacrylamide (EGDMA) as the crosslinker, 2,2′-azobisisobutyronitrile (AIBN) as the initiator to prepare magnetic molecularly imprinted polymer (MMIPs) with specific adsorption of quercetin by surface molecular imprinting technique. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and transmission electron microscope (TEM). The binding properties of MMIPs were evaluated by isothermal adsorption, kinetic adsorption and selective adsorption experiments. The results showed that the saturated adsorption capacity and adsorption time were 17.9 μmol/g and 20 min, respectively, and showed high selectivity for quercetin. Under optimized conditions, the limit of detection (LOD) and limit of quantitation (LOQ) were 55.327 ng/mL and 184.413 ng/mL, respectively. The recovery and RSD were 85.34–95.67% and 3.36–6.59, respectively. We successfully combined magnetic solid phase extraction with high performance liquid chromatography (MSPE-HPLC-UV) for the enrichment, separation and detection of quercetin in rat plasma. The results showed that the peak and half-life of quercetin in plasma were 1.5 h (32.310 μg/mL) and 3 h, respectively. The method was simple, rapid and efficient, and could be applied to the separation and detection of quercetin in complex matrices, and also provides a basis for the separation and identification of other natural chemical components.