Abstract
In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g−1. Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g−1, and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.
Highlights
In this study, a novel method based on genistein magnetic molecularly imprinted polymers (GenMMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier
In comparison with the curve of F e3O4, the new characteristic peaks at 1088 cm−1 were attributed to the Si–O stretching vibration, indicating that SiO2 was coated on the surface of Fe3O4 nanoparticles
The absorption bands at 1710 cm−1 and 2922 cm-1 were due to the stretching vibrations of C=O and C–H in ethylene glycol dimethacrylate (EGDMA), respectively[25], which confirms that the MMIP layer was polymerized on the surface of Fe3O4@SiO2–CH2=CH2
Summary
A novel method based on genistein magnetic molecularly imprinted polymers (GenMMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. Several methods have been reported to determine genistein, including mass spectrometry[4], liquid chromatography[5], gas chromatography[6], and an enzyme-linked immunosorbent a ssay[7]. These methods can effectively identify genistein, real samples exhibit highly complex matrices, so the analysis of genistein requires sample pretreatment methods. Imprinted polymers (MIPs) have been synthesized as new innovative s orbents[12,13,14] and possess excellent characteristics, such as low cost, ease of preparation, excellent reusability and high s electivity[15] Due to their excellent adsorption performance, MIPs have attracted increasing attention[16]. This study aimed to design and synthesize a magnetic molecularly imprinted column that can recognize genistein and establish a detection method for genistein in a complex matrix
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