Enamel Surface Microhardness Research Articles

Effect of whitening dentifrices on dental enamel: an analysis of color, microhardness, and surface roughness in vitro.

This study aimed to evaluate the influence of different whitening toothpastes on color change and alteration in enamel surface roughness and microhardness compared to a conventional toothpaste. Fifty bovine incisors were selected, cleaned, and stored before being divided into five groups: a conventional toothpaste group and three whitening toothpaste groups containing different abrasive agents: silica, hydrogen peroxide, and activated charcoal. Specimens underwent simulated brushing, staining with black tea solution, and subsequent analyses of color, surface roughness, and microhardness. Statistical analysis was performed using three-way ANOVA and Tukey post-hoc tests (P .05). The results showed that the color analysis revealed similar whitening potential among all toothpastes. They showed significant differences in surface roughness (P .001) and microhardness (P .001) after simulated brushing. While all toothpastes caused a decrease in microhardness, the charcoal-based toothpaste showed a significant increase in surface roughness compared to the initial condition. All toothpastes demonstrated whitening capability. Surface roughness changed after brushing with activated charcoal-based whitening toothpaste, but final roughness was similar across all groups. Whitening toothpastes led to a decrease in enamel microhardness, with similar final performance across all toothpastes analyzed.

Assessment of surface microhardness of enamel surface treated with Nano- HAP serum before and after bleaching

Background: Although bleaching is typically considered a safe procedure, various investigations have found minor negative effects and changes in mineral composition. The aim was to Evaluate and compare the efficacy of using Nanohydroxyapatite serum on surface microhardness of enamel surface before and after bleaching with chemically cured Boost bleaching. Material and methods: ten sound human permanent upper and lower premolar teeth were used and their roots were removed 2 mm apically to the cementoenamel junction, the crowns were sectioned mesiodistally into two halves buccal and lingual/palatal, the buccal surface was further subdivided into two halves. The samples were embeded in an acrylic resin, resulting in 30 specimens divided into 3 groups: Control group: using Boost bleaching and stored in artificial saliva for 14 days, prevention group: nanohydroxyaptite (n-HAP) serum applied 2-3 min once daily for 10 days followed by bleaching then stored for 14 days in artificial saliva and treatment group: in which bleaching used before nanohydroxyapatite serum and stored in artificial saliva for 14 days. The samples were subjected to a Vickers microhardness test measured at 4 times: base line, after nanohydroxyapatitem, after one day of bleaching and after storage in artificial saliva for 14 days in all groups. The data were analyzed statistically using repeated analysis of variance (ANOVA) test followed by Tukey's test. Results: there was a significant increase in microhardness in the prevention group (p<0.05) while there was no significant difference in microhardness readings in control and treatment groups (p> 0.05). Conclusion: n-HAP may enhance the microhardness of a bleached enamel surface when used as a preventive & treatment measure. Suggested that a higher increase in enamel microhardness occurs when n-HAP is used for 2-3 min once daily for 10 days before bleaching and maintaining this increase even after storage for 14 days in artificial saliva.

Comparison of Surface Microhardness of Enamel after Bleaching with Two Commercially Available Hydrogen Peroxide-based Bleaching Agents -An In-vitro Study

Comparison of Surface Microhardness of Enamel after Bleaching with Two Commercially Available Hydrogen Peroxide-based Bleaching Agents -An In-vitro Study

Do effervescent vitamin tablets affect the surface roughness, microhardness, and color of human enamel and contemporary composite resins?

To compare the impact of four effervescent vitamin tablets on the surface roughness, microhardness, and color of human enamel and contemporary composite resins. Sixty enamel and a total of 240 anterior (Gradia Direct Anterior), posterior (Gradia Direct Posterior), and universal composite resin (Filtek Ultimate) specimens (n = 60 per group) were fabricated. Each group was subdivided into five subgroups (n = 12, per subgroup). The specimens were subsequently immersed in YOUPLUS,Redoxon, Sunlife Immuvit, and Sambucol effervescent vitamin solutions for 2 min per day over 30 days. Distilled water was used as control. Subsequently, surface roughness (Ra) was detected using a profilometer, and microhardness (VHN) was measured using a microhardness tester. A spectrophotometer device was used to record the L,a,b color coordinates of the specimens after 24 h, 7 days, and 30 days, and the color changes (ΔE00) of the groups were calculated. The data were analyzed by ANOVA, two-way ANOVA, Kruskal-Wallis, Levene's, and Fisher's least significant difference (LSD) tests (p<0.05). SEM analysis was conducted on one randomly selected specimen per group (×1000). In terms of surface roughness, materialXvitamin interactions were found significant (p<0.05). The increase in Ra from 24 h to 30 days was found significant (p<0.05) except for Gradia Direct Anterior X Redoxon, Sunlife Immuvit and Sambucol, Filtek Ultimate X Sunlife Immuvit and Sambucol, and all control groups. Ra changes were also concluded by SEM. Regarding VHNs, material X vitamin interactions were significant (p<0.005), except for all Filtek Ultimate subgroups. The changes in VHNs of the groups from 24 h to 30 days were significant for all enamel and Gradia Direct Anterior X YOUPLUS, Gradia Posterior X YOUPLUS, Sunlife Immuvit, and Sambucol groups (p < 0.05). In terms of ΔE00, significant differences were observed between the 7 days and 30 days in the enamel (p = 0.047), Gradia Direct Anterior (p = 0.019), and Gradia Direct Posterior groups (p = 0.038). Daily consumption of effervescent vitamin tablets can increase surface roughness, decrease microhardness, and influence the color of human enamel and contemporary anterior, posterior, and universal composite resins after a 30-day period.

Comparative Evaluation of Tooth Enamel Surface Microhardness Exposed to Novel Modified Casein Phosphopeptide Amorphous Calcium Phosphate, APF Gel and GC Tooth Mousse

Comparative Evaluation of Tooth Enamel Surface Microhardness Exposed to Novel Modified Casein Phosphopeptide Amorphous Calcium Phosphate, APF Gel and GC Tooth Mousse

Effect of different approaches of direct radiation on the surface structure and caries susceptibility of enamel

It is not clear whether different radiation methods have different effects on enamel. The purpose of this study was to compare the effects of single and fractionated radiation on enamel and caries susceptibility and to provide an experimental basis for further study of radiation‑related caries. Thirty-six caries-free human third molars were collected and randomly divided into three groups (n = 12). Group1 (control group) was not exposed to radiation. Group 2 received single radiation with a cumulative dose of 70 Gy. Group 3 underwent fractionated radiation, receiving 2 Gy/day for 5 days followed by a 2-day rest period, for a total of 7 weeks with a cumulative dose of 70 Gy. Changes in microhardness, roughness, surface morphology, bacterial adhesion and ability of acid resistance of each group were tested. Scanning electron microscope revealed that the enamel surface in both radiation groups exhibited unevenness and cracks. Compared with the control group, microhardness and acid resistance of enamel decreased, while roughness and bacterial adhesion increased in both the single radiation and fractionated radiation groups. Compared with the single radiation group, the enamel surface microhardness and acid resistance decreased in the fractionated radiation group, while roughness and bacterial adhesion increased. Both single radiation and fractionated radiation resulting in changes in the physical and biological properties of enamel, with these changes being more pronounced in the fractionated radiation group. Therefore, fractionated radiation is recommended as a more suitable method for constructing a radiation‑related caries model in vitro.

Effects of experimental in-office bleaching gels incorporated with co-doped titanium dioxide nanoparticles on dental enamel physical properties.

To evaluate the physical properties of enamel submitted to hydrogen peroxide (HP) incorporated with titanium dioxide nanoparticles (NP) co-doped with nitrogen and fluorine and irradiated with violet LED light (LT). Enamel-dentin disks were randomly allocated (T0) into groups, according to HP (HP6, HP15, or HP35) and NP (no NP, 5NP, or 10NP) concentrations, and irradiated or not with LT. A negative control (NC) group was set. After three bleaching sessions (T1, T2, and T3), specimens were stored in saliva for 14days (T4). Enamel surface microhardness number (KHN), surface roughness (Ra), cross-sectional microhardness (ΔS), energy-dispersive spectroscopy (EDS), scanning electron (SEM), and polarized light (PLM) microscopies were performed. Surface KHN was significantly influenced by NP over time, independently of LT irradiation. At T3 and T4, gels with 5NP and 10NP exhibited no KHN differences compared to NC and baseline values, which were not observed under the absence of NP. NP incorporation did not statistically interfere with the ΔS and Ra. PLM images exhibited surface/subsurface darkening areas suggestive of demineralizing regions. SEM demonstrated some intraprismatic affection in the groups without NP. EDS reported a higher enamel calcium to phosphorus ratio following 10NP gels applications. Gels with NP maintained the enamel surface microhardness levels and seemed to control surface morphology, upholding the mineral content. None of the proposed experimental protocols have negatively influenced the enamel surface roughness and the cross-sectional microhardness.

Theobromine for Remineralization of White Spot Lesions on Dental Enamel: A Systematic Review and Meta-analysis.

This systematic review and meta-analysis evaluated theobromine's (Theobroma cacao) potential in remineralizing white spot lesions in dental enamel. Methods: This study is reported according to the PRISMA checklist and was registered in PROSPERO (CRD42023414371). In vitro tests that evaluated the remineralizing potential of theobromine compared to fluoride ion after demineralization for the formation of white spot lesions on enamel were selected, with no limitation on the year of publication. Electronic searches were performed in PubMed/MEDLINE, Scopus, and Web of Science by two independent researchers. Thirty articles were received of which six were selected according to the inclusion criteria. The selected studies evaluated the Enamel Surface Microhardness (SMH), Vickers or Knoop, before and after treatment with theobromine and fluoride solutions. For the SMH Vickers, there were no differences between groups at baseline (p=1.00; mean difference: -0.00; CI: -11.36 to 11.36) and after treatment (p=0.51; mean difference: 4.12; CI: -8.16 to 16.41). The results of SMH Knoop showed differences between groups at baseline, favoring the experimental group (p=0.002; mean difference: 9.84; CI: 3.65 to 16.02) and after treatment favoring the control group (p=0.00001; mean difference: -5.45; CI: -7.62 to -3.27). The use of theobromine increases the microhardness of dental enamel subjected to a demineralization process, thus being effective in the remineralization of this tissue with success equivalent to that obtained with the use of fluoride.

Comparative Evaluation of Dental Enamel Microhardness Following Various Methods of Interproximal Reduction: A Vickers Hardness Tester Investigation.

Interproximal enamel reduction, also known as stripping, is a common orthodontic procedure that reduces the mesiodistal diameter of teeth, allowing for a balance of available space in dental arches. The aim of this study was to assess the enamel surface microhardness resulting from the application of currently available methods for interproximal reduction. Forty-two extracted human permanent teeth were divided into six different groups, each subjected to a therapeutic stripping procedure using various methods (i.e., diamond burs, abrasive strips of 90 μm, 60 μm, 40 μm, and 15 μm, and abrasive discs). Stripping was performed by a single individual in accordance with the manufacturers' recommendations for the various systems used. One of the proximal faces of the tooth underwent IPR, while the other side remained untreated for control. The hardness of the enamel surface was measured using a Vickers hardness tester. The control group achieved the hardest enamel surface (354.4 ± 41.02 HV1), while the lowest was observed for enamel surfaces treated with 90 µm abrasive strips (213.7 ± 118.6). The only statistically significant difference was identified in comparisons between the values measured for the control group and those obtained after stripping with diamond burs (p = 0.0159). Enamel microhardness varied depending on the stripping instrument used, but no statistically significant differences were found (p > 0.05). Optimal microhardness values, close to those of healthy enamel, were achieved after mechanical treatment with 15 µm abrasive strips and abrasive discs. Dental stripping is a safe therapeutic procedure that has a relatively minor influence on the microhardness of surface enamel.

Comparison of the effects of fluoride varnish containing silver nanoparticles and conventional fluoride varnish on the surface microhardness of tooth enamel.

Nano-silver fluoride (NSF) has been introduced to improve enamel lesions. The effective use of varnishes is important in the prevention of dental caries. The study aimed to compare the effect of conventional sodium fluoride varnish with the same varnish containing 1% and 2% silver nanoparticles (AgNP) on the surface microhardness of enamel. The baseline surface microhardness of 40 premolar teeth was measured using a Vickers microhardness tester. After immersing the samples in a demineralizing agent for 24 h, the microhardness was measured again. In group B, a layer of conventional fluoride varnish was applied to the tooth surfaces using a microbrush with soft bristles, following the manufacturer's instructions. Groups C and D were treated with 1% and 2% NSF varnishes, respectively, while group A received no varnish. Surface microhardness tests were conducted on all specimens, including those previously tested. The microhardness of the enamel surface increased significantly in all 3 test groups compared to the microhardness after demineralization (p < 0.05). Conventional fluoride varnish and fluoride varnishes containing 1% and 2% AgNP are equally effective in remineralizing initial caries.

The effect of combined use of resin infiltration with different bioactive calcium phosphate-based approaches on enamel white spot lesions: An in vitro study

ObjectivesThis in vitro study aimed to evaluate the effect of resin infiltration combined with casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF) or bioactive glass (BAG) on the stability of enamel white spot lesions (WSLs) treatment. Materials and methodsEighty-four enamel blocks were prepared from the buccal surfaces of sound human premolars. All enamel blocks were placed in a demineralisation solution for 3 days to establish the artificial enamel WSLs. Enamel blocks with WSLs were randomly divided into three groups (n = 28 each group): RI/B: one-off resin infiltration followed by twice daily BAG treatment; RI/C: one-off resin infiltration followed by twice daily CPP-ACPF treatment; RI: one-off resin infiltration treatment only (as control) and subjected to pH cycling for 7 days. Surface morphology, elemental analysis, crystal characteristics, surface roughness and microhardness of enamel surfaces were investigated by scanning electron microscopy and energy-dispersive spectrometry observation, X-ray diffraction (XRD), atomic force microscope and Vickers’ hardness testing, respectively. ResultsMean values of the surface roughness (mean±standard deviation (nm)) were 24.52±5.07, 27.39±5.87 and 34.36±4.55 for groups RI/B, RI/C and RI respectively (p = 0.003). The calcium to phosphate ratios were 1.32±0.16, 1.22±0.26 and 0.69±0.24 for groups RI/B, RI/C and RI respectively (p < 0.001). XRD revealed apatite formation in all three groups. The mean enamel surface microhardness (kg/mm2) of the groups were 353.93±28.49, 339.00±27.32 and 330.38±22.55 for groups RI/B, RI/C and RI respectively (p = 0.216). ConclusionsResin infiltration combined with CPP-ACPF or BAG remineralisation appears to improve the surface properties of WSLs. Clinical SignificanceThe combination of resin infiltration and CPP-ACPF/BAG remineralisation may be a potential treatment for the management of the WSLs.

Development of toothpaste formulations containing mineral clays as abrasive agents and their effects on the physical properties of dental enamel.

To assess the physical properties and topographical aspect of dental enamel's surface microhardness (KHN), surface roughness (Ra), and color parameters CIELAB (∆Eab*), CIEDE 2000 (∆E00), and whiteness index for dentistry (∆WID) after toothbrushing with experimental toothpaste formulations with the following mineral clay types: kaolin, Sparclay SGY, and Tersil CGY used as abrasive component. Scanning electron microscopy (SEM) was performed for topographical analyses purposes. A total of 96 bovine incisors were used in the experiment. They were divided into eight experimental groups (n = 12), namely: NC-negative control (no treatment), CT12-Colgate Total 12®, CMP-Colgate® Máxima Proteção Anticáries, K-kaolin experimental toothpaste, SGY-Sparclay SGY experimental toothpaste; CGY-Tersil CGY experimental toothpaste, SD-SiO2 experimental toothpaste, and CC-CaCO3 experimental toothpaste. All samples were subjected to mechanical brushing protocol with 5000 cycles and kept in artificial saliva with daily exchanges. KHN was analyzed through the mixed linear model for repeated measures over time. Ra was analyzed through nonparametric Kruskal Wallis and Dunn tests to compare the groups. Paired Wilcoxon test was run to compare experimental times. ∆WID, ∆Eab*, and ∆E00 were analyzed through Kruskal Wallis and Dunn tests. All analyses were performed in R* software, at 5% significance level. EXP_SGY recorded higher KHN than EXP_SiO2 and EXP_CaCO3, whereas EXP_K showed increased Ra in comparison to CMP (p = 0.0229). ∆Eab and ∆E00 were significantly higher in the CT12, EXP_SiO2, and EXP_CaCO3 groups than in the NC and EXP_K (p < 0.0001). There were no significant changes in ∆WID (p = 0.0852). According to SEM results, toothbrushing with experimental toothpastes added with mineral clay types did not have significant impact on enamel's polishing and smoothness. CLINICAL RELEVANCE: Mineral clays have a broad application in the cosmetic industry, and recently, they have been used in the formulation of vegan toothpaste.

Evaluation of the remineralizing effect of the chicken eggshell paste after removal of the fixed orthodontic appliance: An in vitro study.

Demineralization of the enamel surface, which appears as white spot lesions during and after removal of the fixed orthodontic appliance, is the most common disadvantage of the orthodontic treatment course. Using the remineralizing agents during and after orthodontic treatment helps to avoid those enamel defects. The present study aims to assess the remineralizing effect of the chicken eggshell powder on the demineralized enamel surfaces after debonding the orthodontic bracket system. The current study was performed on 80 prepared premolar crowns embedded into acrylic molds. The samples were prepared to receive routine steps of the bonding process for the bracket system. The paste of the chicken eggshell powder was added to the samples after the debonding process. Scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) were used to evaluate the remineralization effect of the chicken eggshell powder. Also, the Vickers microhardness tester was used to assess the enamel surface microhardness. It was found that the mean value of the Ca/P ratio for the samples before bonding of the orthodontic bracket system was (4.17 ± 2.2). This value significantly decreased to (2 ± 1.3) after debonding of the orthodontic bracket system and then showed a significant increase to (4.79 ± 2.65) after remineralization. These results were assured by the values of the Vickers microhardness tester. The chicken eggshell powder has an excellent remineralization effect for the demineralized enamel surface after debonding the orthodontic enamel surface.

Comparative evaluation of probiotic solutions on surface roughness and microhardness of different restorative materials and enamel.

This research study aimed to investigate the impact of probiotic mouthwash and kefir on the surface characteristics, specifically surface roughness and microhardness, of different restorative materials, as well as permanent and deciduous tooth enamels. Thirty disc-shaped specimens were prepared from composite resin (G-ænial Posterior (GP)), polyacid-modified composite resin (compomer) (Dyract-XP (DXP)), and resin-modified glass ionomer cement (Ionoseal (IS)). Additionally, thirty specimens of enamel were obtained from permanent teeth (PT) and thirty from deciduous teeth (DT) by embedding buccal and lingual sections, acquired through vertical sectioning of 15 permanent and 15 deciduous human tooth crowns in the mesiodistal orientation within acrylic resin blocks. The specimens were then categorized into three distinct groups and immersed for 14 days in one of the following solutions: distilled water, kefir or probiotic mouthwash. The mean surface roughness values of all specimens were assessed using an atomic force microscope, while the mean surface microhardness was measured using a Vickers hardness measuring instrument. The results revealed a statistically significant difference in mean surface roughness among the various restorative materials (p < 0.001). Among the restorative materials, the IS material exhibited notably higher mean surface roughness values than other restorative materials and tooth enamel, while no significant differences were observed between the PT and DT groups. Importantly, the main effect of the solutions under investigation was not statistically significant (p = 0.208). No significant difference was found between the surface roughness values of specimens subjected to the different solutions. When evaluating the effects of materials and solutions on microhardness, the main effects of material and solution variables and the influence of material-solution interactions were statistically significant (p < 0.001). Taken together, these results indicate that consistent use of kefir or probiotic mouthwashes may impact the surface properties of various restorative materials and tooth enamel.

A new role for resveratrol: Protection of enamel against erosion

ObjectiveThe aim of this study was to determine the effect of different concentrations of resveratrol in protecting enamel against initial dental erosion in vitro. MethodsNinety bovine enamel samples (4 × 4 mm) were divided into six groups: Phosphate buffered saline (negative control; PBS), Commercial solution (Elmex Erosion Protection™; positive control) and resveratrol at 4 different concentrations (1, 10, 100 or 400 µg/mL). Initially, the samples were incubated in saliva for the formation of the acquired pellicle (250 µL, 1 h, 37 °C, 250 rpm). Afterward, the samples were incubated in the respective treatments (250 µL, 1 min, 37 °C, 250 rpm) and then reincubated in saliva (250 µL, 1 h, 37 °C, 250 rpm). Finally, the samples were subjected to an erosive challenge by incubating in 1 % citric acid (1 mL, pH 3.5, 1 min, 25 °C, 250 rpm). The percentage surface microhardness change (% SMC) was assessed using a microhardness tester. Data were analyzed by Kruskal-Wallis and Dunn's tests (p < 0.05). ResultsThe treatments with Elmex™ and resveratrol (1, 10 and 100 µg/mL) significantly protected enamel compared to the negative control, without significant differences among them. However, the group treated with the highest resveratrol concentration (400 µg/mL) did not show a significant difference from the negative control. ConclusionsResveratrol at concentrations ranging from 1 to 100 µg/ml was effective in preventing loss of enamel surface microhardness. Clinical significanceThis result suggests a potential new direction for the development of dental products based on resveratrol for the prevention of dental erosion.

CAD/CAM leucite-reinforced glass-ceramic for simulation of attrition in human enamel in vitro

ObjectiveInvestigate attrition simulation using CAD/CAM leucite-reinforced glass-ceramic antagonists on occlusal vs. buccal enamel. MethodsThree dental materials with known wear rates (resin-modified glass-ionomer, micro-filled, and fine particle composites) validated the wear simulator (CAD/CAM glass-ceramic antagonists, 200 cycles, 80 N load, deionised water irrigation, 0.7 mm sliding movement). Following this, human molars were sectioned into paired occlusal and buccal polished samples (n = 8/gp). Exposed 1.5 mm Ø enamel areas were subjected to attritional wear with and without pre-immersion in citric acid (5 min, 0.3%, pH 3.8). Profilometry measured step-height enamel wear and surface microhardness at different depths was calculated using Vickers indentation at 0.1 N and 0.5 N loads. ResultsDental material wear using the CAD/CAM antagonists showed consistency with previous data: mean (SD) resin-modified glass ionomer material loss of 177.77 (16.89) µm vs. 22.15 (1.30) µm fine particle hybrid composite resin wear vs. 13.63 (1.02) µm micro filled composite resin wear (P < 0.001). The coefficient of variation was less than 10%. Following validation, enamel sample wear was significantly increased when attrition was introduced (P < 0.001) independent of buccal vs. occlusal sample location (P < 0.05). Attrition resulted in occlusal wear of 26.1 ± 4.5 µm vs. buccal 26.3 ± 1.2 µm and attrition/erosion resulted in occlusal wear of 26.05 ± 4.46 µm vs. buccal 25.27 ± 1.16 µm. Whereas erosion-alone resulted in occlusal wear of 1.65 ± 0.13 µm and buccal 1.75 ± 0.03 µm. Microhardness testing at different loads revealed significantly greater hardness reductions in occlusal enamel vs. buccal enamel for 0.1 KgF indentations (P < 0.001) whereas in contrast 0.5 KgF indentations showed no differences. SignificanceWear simulation with CAD/CAM glass ceramic antagonists produced consistent wear in dental materials and human enamel, regardless of enamel surface origin. Lighter (0.1 KgF) hardness testing of occlusal vs. buccal origin revealed damage to the mechanical integrity of the superficial worn enamel.

Effects of topical fluoride on primary tooth enamel microhardness after diode laser treatment

ObjectivesTooth decay is controlled and prevented using fluorides. Specifically, a combination of laser irradiation and topical fluoride application can improve fluoride absorption. Although laser irradiation is recommended in traditional technology for caries prevention, little is known about the efficacy of caries-inhibiting laser diodes. Further, most studies have focused on permanent teeth. The objective of this study was to explore the effect of laser diode radiation mixed with topical fluoride on the enamel surface microhardness of primary teeth. Material and methodThe 60 primary teeth were divided into 60 plates. Baseline Vickers microhardness was established for each enamel surface. The samples were then divided into three groups. 4 % NaF varnish (group A) and 4 % NaF varnish using diode 2 and 3 W lasers (groups B and C, respectively). The final microhardness was measured and statistically evaluated using SPSS version 16. ANOVA was used to compare the means of the tested groups, which had a P-value of 0.05, indicating significance. ResultThe mean and standard deviation of microhardness for the different groups was tested. Group C (4 % NaF with a diode laser at 3 W) showed a higher average Vickers hardness (VHN) than Group B (4 % NaF with a diode laser at 2 W), whereas group A showed the lowest average VHN. There was a significant difference in microhardness between groups (P 0.05). ConclusionThis study explored the effect of laser mixed with topical fluoride on enamel surface microhardness of primary teeth. The use of a diode laser (Quanta System, Italy) with a fluoride varnish applied to the enamel surface has a greater effect on the resistance of the enamel to caries.

In vitro Evaluation of Remineralization Efficacy of Fluoridated and Nonfluoridated Toothpastes on Demineralized Enamel Surface

Aim: The aim of the present study was to compare the remineralization efficacy of fluoridated and nonfluoridated toothpaste on artificially demineralized enamel. Materials and Methods: Buccal surface of eighty extracted human premolars was treated with demineralizing solution for 96 h and they were randomly distributed into four groups. Group 1 was control, Group 2 was brushed with Xyliwhite toothpaste (fluoride free), Group 3 was brushed with Superbee Propolis toothpaste (fluoride free), and Group 4 was brushed with Amflor Pro toothpaste (Fluoridated). Brushing was performed for 2 min twice daily with a powered toothbrush for 7 days. After every brushing, the specimens were cleaned and stored in artificial saliva at room temperature. The enamel surface microhardness was measured with Vickers hardness test postdemineralization and posttreatment and a comparative analysis was made. Results: One-way analysis of variance F-test showed a statistically significant difference in remineralization efficacy among the groups (P &lt; 0.001). Tukey’s post hoc test showed that Group 2, Group 3, and Group 4 have higher remineralization efficacy as compared to control (P &lt; 0.001). Maximum remineralization efficacy was seen with Group 4. Conclusion: Fluoridated and nonfluoridated toothpastes have the potential to remineralize the demineralized enamel surface.

The Effect of Two Types of Erbium Laser and Light Cure Fluoride Varnish on Enamel Demineralization and Surface Microhardness around Metal Orthodontic Brackets: An in-Vitro study

The Effect of Two Types of Erbium Laser and Light Cure Fluoride Varnish on Enamel Demineralization and Surface Microhardness around Metal Orthodontic Brackets: An in-Vitro study

Comparative Evaluation of the Remineralizing Potential of Silver Diamine Fluoride, Casein Phosphopeptide-amorphous Calcium Phosphate, and Fluoride Varnish on the Enamel Surface of Primary and Permanent Teeth: An In Vitro Study.

With the paradigm shift in the management of dental caries, the focus is now laid on remineralization therapies that can arrest the progression of the disease and remineralize the subsurface lesions. The purpose of this study was to determine and compare the remineralizing potential of silver diamine fluoride (SDF), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and fluoride varnish (FV) on enamel surfaces in primary and permanent teeth. A total of 120 primary anterior teeth and 120 premolars were used to prepare enamel blocks in acrylic resin. The mean baseline surface microhardness (SMH) for each sample was determined using a microvickers hardness testing machine. Thereafter, the samples were randomly and equally distributed into groups and subgroups based on the materials used, that is, SDF, CPP-ACP, FV, and distilled water (control). After subjecting the samples to a pH cycling regime, SMH was determined again and the percentage change in SMH was calculated. The data were tabulated and subjected to statistical analysis using an independent t-test and one-way analysis of variance (ANOVA). In primary teeth, the least mean percentage reduction in SMH was observed after the application of FV followed by SDF, CPP-ACP, and control. In permanent teeth, both SDF and FV showed the least percentage reduction of enamel SMH followed by CPP-ACP and control. Silver diamine fluoride (SDF), FV, and CPP-ACP showed remineralizing potential in both primary and permanent teeth. Kaur S, Bhola M, Bajaj N, et al. Comparative Evaluation of the Remineralizing Potential of Silver Diamine Fluoride, Casein Phosphopeptide-amorphous Calcium Phosphate, and Fluoride Varnish on the Enamel Surface of Primary and Permanent Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2023;16(S-1):S91-S96.