Posterior capsular opacification (PCO), known as a prevalent complication of cataract surgery, occurs because of the attachment and abnormal proliferation of remaining lens epithelial cells (LECs) after cataract surgery. To reduce the PCO occurrence by improving cell adhesion resistance, we attempted zwitterionic sulfobetaine methacrylate (SBMA) coating through ultraviolet-induced graft polymerization on the surfaces of intraocular lens (IOL) materials. In this study, the effect of the concentration of SBMA monomers in the coating solutions adjusted from 10 wt% to 30 wt% was investigated on the chemical composition, morphology, and hydrophilicity of the IOL material surfaces modified with SBMA. Additionally, the effect of SBMA concentration on preventing adhesion to protein and LECs on the surfaces of SBMA-modified IOL materials was evaluated in vitro. Grafting of zwitterionic SBMA on the surfaces of hydrophobic acrylic IOL materials was confirmed by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and water contact angle (WCA). To evaluate the long-term hydrophilicity of coating films prepared with different concentrations of SBMA on the IOL material surfaces, the WCA of IOL materials stored in phosphate-buffered saline solution was measured as a function of time. The coating film prepared at a concentration of 20 wt% of SBMA showed the lowest WCA after 28 days, showing the best long-term hydrophilicity. Also, that prepared at a concentration of 20 wt% of SBMA exhibited the highest anti-adhesion effect on proteins and LECs.