Surface Acoustic Wave Biosensor Research Articles

Specific targeting of ultrasound contrast agent (USCA) for diagnostic application: an in vitro feasibility study based on SAW biosensor

The present study described a new strategy to examine the interaction between the targeted ultrasound contrast agent (USCA) and its target under flow conditions with a surface acoustic wave (SAW) transducer. The sensing principle is based on the measurement of the phase change on the sensing element upon the binding of specific biomolecules. Love-wave biosensor array was consisting of sensor elements and reference elements. The sensor elements have been prepared by coating the sensor surface with tumor marker EDB-fibronectin by means of SAM technique and carbodiimide chemistry. Reference elements were left blank or coated with fibronectin and used to eliminate thermal drift, unspecific binding, and turbulence from injection of liquids by calculating the differential phase shift with respect to the sensor elements. The binding of targeted USCA to the sensor surface was constantly recorded by monitoring the phase shift on the sensor element. The binding of targeted USCA generated a high phase shift on the sensor elements, but almost no change on the reference elements. Control experiments using non-targeted and isotype-targeted USCA confirmed the specificity of binding due to anti-EDB-fibronectin scFv-antibody-fragment–EDB-fibronectin antigen interaction. The suitability of the SAW technique to monitor the specific binding behavior of targeted micron-sized USCA in real time has been well established.

Improvement of surface acoustic wave gas and biosensor response characteristics using a capacitive coupling technique.

As for many other electronic devices and circuits, electrical contact to surface acoustic wave sensors is usually made using bonding wires. This technique is known to result in reliable contact under most conditions, but it does so with several disadvantages. First, electrical contact is not reversible, impeding replacement of sensor devices. Second, bonding wires are quite delicate and should not be exposed to high gas or liquid flows. Third, the presence of bonding wires may limit the potential to miniaturize a sensor housing or flow cell. Therefore, a capacitive coupling technique was developed to replace bonding wires. This permitted redesign of flow cells and sensor arrays, resulting in flow cell volumes of 80 microL to 60 nL. As a consequence, response times were reduced to 1-2 s in gas sensing and a few minutes in liquid sensing, respectively. At the same time, sensor devices can be easily replaced, and the system is less susceptible to malfunction.

A surface acoustic wave biosensor concept with low flow cell volumes for label-free detection.

Special surface acoustic wave (SAW) devices using horizontally polarized surface shear waves can be operated in water. They allow an easy detection of molecules with biological relevance (e.g., proteins) via direct detection of the adsorbed mass. The transducer structures of conventional SAW devices are usually connected to the electronics by bond wires. In consequence, flow cell volumes can hardly be designed smaller than 50 microL. A new type of SAW device that is coupled capacitively with the electronics enables the reduction of flow cell volumes down to 60 nL, which decreases sample consumption and reduces the length of the measurement cycles down to a few minutes. To create an immunosensor, the SAW devices first are coated with a thin parylene layer for creating a sensor surface that is chemically homogeneous. Then OptoDex, a dextran containing both photoactive and functional groups is immobilized photochemically. Finally, antibodies are coupled via conventional EDC/NHS chemistry. The technique has been used to monitor urease binding at anti-urease-coated SAW devices in real time and with good resolution. Because of the simple sensor handling and the economical sample use, the new SAW device is particularly suitable for the design of an array.

Covalent bound sensing layers on surface acoustic wave (SAW) biosensors

This paper reports on the development of immunosensors based on commercially available surface acoustic wave (SAW) devices working at 380 MHz. Approaches for coating the sensor surface with a sensing layer of receptive biomolecules are presented and discussed. It was found that the sensitivity strongly relates to the immobilization method. Additionally, the sensitivity can be influenced by the density of accessible biomolecules on the active sensing area. Usually, by most of the standard immobilization procedures, two-dimensional layers of receptive biomolecules are obtained. We present a three-dimensional layer, which provides a higher absolute amount of recognition molecules. A dextran layer is photoimmobilized to the sensor surface and the recognition molecules are covalently embedded into the dextran matrix. The feasibility of specific immunosensing is investigated using SAW sensors connected to a fluid handling system.

A comparison of protocols for the optimisation of detection of bacteria using a surface acoustic wave (SAW) biosensor

A dual channel surface acoustic wave (SAW) device has been used as a biosensor to detect two different microorganisms, Legionella and Escherichia coli, simultaneously. A series of experiments was conducted to optimise the use of the SAW for bacterial detection using a novel protocol of coating bacteria on the sensor surface prior to addition of the antibody. Results were compared with an experiment in which a conventional protocol was utilised, where antibody was coated on the sensor surface prior to exposure to bacteria. The concentration of bacteria that attached to the surface of the SAW device was related to the antibody that specifically bound to it and therefore to frequency in a dose dependent fashion. Unlike conventional microbiological techniques quantitative results can be obtained for Legionella and E. coli down to 10 6 cells per ml within 3 h. In addition E. coli was detected down to 10 5 cells per ml in a modified protocol using sheep IgG as a blocking agent.

New Biosensor Using Shear Horizontal Surface Acoustic Wave Device

This paper describes a new biosensor to detect an enzyme reaction in liquid using surface acoustic wave (SAW) devices fabricated on 36°-rotated Y-cut, X-propagating LiTaO3. The sensing wave on the substrate is a predominantly shear-horizontal-mode SAW (SH-SAW) and is affected by a strong acoustoelectric interaction between the piezoelectric potential and electrical properties of the materials in the adjacent liquid. As an example of an electrical property, pH change associated with an enzyme reaction leads to measurable perturbation in the wave-propagation characteristic. Taking advantage of this phenomenon we realized a SAW biosensor which consists of an immobilized urease membrane on the surface. Also, highly sensitive detection for the urea solution was obtained in our preliminary experiments.