The relation between cancer and inflammation has been widely discussed, and the onset of inflammation in tumor microenvironment promotes the progression of cancer is generally recognized. Tumor necrosis factor (TNF) α, a major inflammatory cytokine, which exists in tumor microenvironment and is constitutively produced by both stromal cells and malignant cells. The implication of TNFα in tumor growth and metastasis has been reported in several literatures. In addition, TNFα causes tissue factor (TF) upregulation in several cell types, including monocyte, endothelial cells, and cancer cells that may contribute to tumor‐associated coagulopathy and cancer metastasis. 4β‐hydroxywithanolide E (4HW) is a natural compound isolated from an edible plant, Physalis peruviana. 4HW has been reported to exhibit promising anti‐cancer and anti‐inflammatory activities. In the present study, we examined the effect of 4HW on TNFα‐induced TF expression and procoagulant activity in two non‐small cell lung cancer cell lines, A549 and H1299. At the non‐cytotoxic concentrations, 4HW potently prevented TF expression caused by TNFα in both cell lines. The procoagulant activity of TNFα‐induced TF was further studied by using plasma clotting assay. The plasma clotting time in the presence of TNFα‐treated cancer cells was obviously shorter than control group, and this phenomenon was successfully reversed by pretreating with either 4HW or anti‐TF antibody. Our results suggest that 4HW might have the potential to treat tumor‐associated thrombosis and inflammation.Support or Funding InformationThis research was supported by Ministry of Science and Technology (MOST 106‐2320‐B‐037‐003‐MYB) and Kaohsiung Medical University (KMU‐TP105H03)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.