Suppress Fusarium Wilt Disease Research Articles

Exploring the Potential of Bacillus subtilis IS1 and B. amyloliquificiens IS6 to Manage Salinity Stress and Fusarium Wilt Disease in Tomato Plants by Induced Physiological Responses.

The intensified concerns related to agrochemicals' ecological and health risks have encouraged the exploration of microbial agents as eco-friendly alternatives. Some members of Bacillus spp. are potential plant-growth-promoting agents and benefit numerous crop plants globally. This study aimed to explore the beneficial effects of two Bacillus strains (B. subtilis strain IS1 and B. amyloliquificiens strain IS6) capable of alleviating the growth of tomato plants against salinity stress and Fusarium wilt disease. These strains were able to significantly promote the growth of tomato plants and biomass accumulation in pot trials in the absence of any stress. Under salinity stress conditions (150 mM NaCl), B. subtilis strain IS1 demonstrated superior performance and significantly increased shoot length (45.74%), root length (101.39%), fresh biomass (62.17%), and dry biomass (49.69%) contents compared to control plants. Similarly, B. subtilis strain IS1 (63.7%) and B. amyloliquificiens strain IS6 (32.1%) effectively suppressed Fusarium wilt disease and significantly increased plant growth indices compared to the pathogen control. Furthermore, these strains increased the production of chlorophyll, carotenoid, and total phenolic contents. They significantly affected the activities of enzymes involved in antioxidant machinery and the phenylpropanoid pathway. Hence, this study effectively demonstrates that these Bacillus strains can effectively alleviate the growth of tomato plants under multiple stress conditions and can be used to develop bio-based formulations for use in the fields.

Rhizobacteria Induce Growth Promotion and Fusarium Wilt Disease Suppression in Watermelon

Watermelon (Citrullus lanatus) cultivation is important for Malaysia's agricultural sector, accounting for approximately 8.8% of tropical fresh fruit production in 2021, primarily concentrated in Kelantan, Pahang, Johor, and Terengganu. Production dropped from 2016 to 2021, but showed resilience recently, bouncing back to 136 kilotonnes in 2022. Watermelon serves as a key export commodity, reaching markets in Singapore, China, and the Middle East. However, Fusarium wilt, caused by Fusarium oxysporum f.sp. niveum (Fon), poses significant challenges, resulting in yield losses ranging from 30% to 80%. The disease thrives in warm, moist climates and can persist in soil for years, posing a long-term threat to cultivation. Management strategies like using resistant crop varieties, rotating crops, and cultural practices are used, but chemical control is limited by environmental concerns and other restrictions. Continuous watermelon cultivation depletes soil nutrients and increases pathogen buildup due to the crop exhausting essential nutrients and promoting disease-causing organisms, thereby reducing soil health and crop yield. Plant growth-promoting rhizobacteria (PGPR) show promise in enhancing plant growth, suppressing pathogens, and improving soil sustainability. This study aims to evaluate the effectiveness of selected rhizobacteria species in promoting watermelon growth and suppressing Fusarium wilt in vitro. The tested rhizobacteria were identified as Pseudomonas atagonensis, P. resinovorans, P. germanica, P. alcaligenes, and P. glycinae. The production of fluorescence pigments was assessed using King's B medium and were detected in P. atagonensis and P. glycinae. The bacteria were further evaluated for their plant growth-promoting abilities, revealing that P. atagonensis, P. resinovorans, P. germanica, and P. glycinae exhibit nitrogen fixation, phosphate solubilization, and potassium solubilization activities. Fusarium wilt disease suppression was assessed via dual culture techniques by measuring the inhibition zone. Results indicated that P. atagonensis exhibited the highest inhibition zone (10 mm), followed by P. glycinae (5.3 mm) and P. alcaligenes (4 mm) compared to the control. Overall, this study suggests that Pseudomonas species can induce growth promotion and suppress Fusarium wilt disease in watermelon.

Comparative Effect of Seed Coating and Biopriming of Bacillus aryabhattai Z-48 on Seedling Growth, Growth Promotion, and Suppression of Fusarium Wilt Disease of Tomato Plants.

Beneficial plant microbes can enhance the growth and quality of field crops. However, the benefits of microbes using cheap and efficient inoculation methods are still uncommon. Seed coating with biocontrol agents can reduce the amount of inocula along with having the potential for large-scale application. Hence, in this research work, the comparative potential of tomato seed coating and biopriming with Bacillus aryabhattai Z-48, harboring multiple plant-beneficial traits, to suppress Fusarium wilt disease along with its beneficial effect on seedling and plant growth promotion was analyzed. Among two bacterial strains, B. aryabhattai Z-48 was able to antagonize the mycelial growth of Fusarium oxysporum f.sp. lycopersici in vitro and its application as a seed coating superiorly benefited seedling traits like the germination percentage, vigor index, and seedling growth index along with a reduced germination time. The seed coating with B. aryabhattai Z-48 resulted in significant increases in the shoot length, root length, dry biomass, and total chlorophyll contents when compared with the bioprimed seeds with the same bacterial strain and non-inoculated control plants. The seed coating with B. aryabhattai Z-48 significantly reduced the disease index (>60%) compared with the pathogen control during pot trials. Additionally, the seed coating with B. aryabhattai Z-48 resulted in a significantly higher production of total phenolics, peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase enzyme in tomato plants. The GC/MS-based non-targeted metabolic profiling indicated that the seed coating with B. aryabhattai Z-48 could cause large-scale metabolite perturbations in sugars, sugar alcohols, amino acids, and organic acids to increase the fitness of tomato plants against biotic stress. Our study indicates that a tomato seed coating with B. aryabhattai Z-48 can improve tomato growth and suppress Fusarium wilt disease effectively under conventional agricultural systems.

Soil amendments with nanoparticles control cucumber wilt caused by Fusarium oxysporium

Soil amendments with nanoparticles control cucumber wilt caused by Fusarium oxysporium

Intercropping with Trifolium repens contributes disease suppression of banana Fusarium wilt by reshaping soil protistan communities

Intercropping with Trifolium repens contributes disease suppression of banana Fusarium wilt by reshaping soil protistan communities

The Imperative Use of Bacillus Consortium and Quercetin Contributes to Suppress Fusarium Wilt Disease by Direct Antagonism and Induced Resistance.

Fusarium wilt diseases severely influence the growth and productivity of numerous crop plants. The consortium of antagonistic rhizospheric Bacillus strains and quercetin were evaluated imperatively as a possible remedy to effectively manage the Fusarium wilt disease of tomato plants. The selection of Bacillus strains was made based on in-vitro antagonistic bioassays against Fusarium oxysporum f.sp. lycoprsici (FOL). Quercetin was selected after screening a library of phytochemicals during in-silico molecular docking analysis using tomato LysM receptor kinases "SILKY12" based on its dual role in symbiosis and plant defense responses. After the selection of test materials, pot trials were conducted where tomato plants were provided consortium of Bacillus strains as soil drenching and quercetin as a foliar spray in different concentrations. The combined application of consortium (Bacillus velezensis strain BS6, Bacillus thuringiensis strain BS7, Bacillus fortis strain BS9) and quercetin (1.0 mM) reduced the Fusarium wilt disease index up to 69%, also resulting in increased plant growth attributes. Likewise, the imperative application of the Bacillus consortium and quercetin (1.0 mM) significantly increased total phenolic contents and activities of the enzymes of the phenylpropanoid pathway. Non-targeted metabolomics analysis was performed to investigate the perturbation in metabolites. FOL pathogen negatively affected a range of metabolites including carbohydrates, amino acids, phenylpropanoids, and organic acids. Thereinto, combined treatment of Bacillus consortium and quercetin (1.0 mM) ameliorated the production of different metabolites in tomato plants. These findings prove the imperative use of Bacillus consortium and quercetin as an effective and sustainable remedy to manage Fusarium wilt disease of tomato plants and to promote the growth of tomato plants under pathogen stress conditions.

Potential of local Bacillus spp. isolates as wilt disease biocontrol agents for Fusarium oxysporum f. sp. cepae on Allium x wakegi

Abstract. Asrul. 2023. Potential of local Bacillus spp. isolates as wilt disease biocontrol agents for Fusarium oxysporum f. sp. cepae on Allium x wakegi. Biodiversitas 24: 4989-4997. Implementation of biological agents for eradication and inhibition of plant pathogens is often ineffective due to its protracted consequences. The objective of this research was to examine the potential of local isolates of Bacillus spp. as a biocontrol agent in suppressing Fusarium wilt disease (Fusarium oxysporum f. sp. cepae) on wakegi onions (Allium × wakegi Araki). The research was conducted in a completely randomized design (CRD) with the treatment of rhizospheric bacterial isolates. The treatments included control (without isolate application) and seven bacterial isolates, namely KP17, KP5, DB9, DB12, DB18, DG4 and DG11. Each treatment was repeated five times and each replication consisted of 10 wakegi onion plants. The results showed that a total of 46 isolates were isolated from the rhizosphere of wakegi onion and seven isolates among them were selected as biocontrol agents. These isolates had similarities with Bacillus spp. in accordance with the colony morphology, physiology and biochemical characteristics. Among the isolates found, DB12 emerged as the isolate that had the potential to be developed as a biocontrol agent.

Double action plant growth promotion microorganisms in suppressing Fusarium wilt disease and increase tomato production

Fusarium oxysporum f.sp lycopersici is a fungus that causes wilt disease in tomatoes, attacking nurseries to mature plants. This study aims to examine several plant growth-promoting microorganisms (PGPM) to control Fusarium wilt and increase tomato production. The study was designed with a completely randomized design in a screen house with four treatments and three experimental units. The treatments tested were mycorrhiza 5 g per plant (M), a mixed solution containing Bacillus sp., Pseudomonas sp., and Trichoderma sp. 10 ml per plant (PGPM1), and combination treatment M and PGPM1 (PGPM2). The study showed a more extended incubation period of the pathogen-causing diseased plants with the treatment of PGPM2 compared to other treatments. The lower intensity of wilt disease was also found in the PGPM2 treatment. Consistently the same treatment also had a better effect on the number of leaves, plant height, and fresh tomato fruit production than other treatments. This study showed a significant effect of PGPM in controlling Fusarium wilt and increasing tomato production.

Exploration, Screening, and Application of Silica Solubilizing Bacteria and Silica Fertilizer to Suppress Fusarium Wilt Disease in Abaca

Pisang abaka (Musa textilis) merupakan penghasil serat alam berkualitas tinggi dengan permasalahan utama penyakit layu fusarium oleh Fusarium oxysporum f. sp. cubense TR4. Penelitian ini bertujuan memperoleh bakteri pelarut silika (BPS), menentukan pengaruh aplikasi BPS dan pupuk silika untuk menghambat serangan fusarium serta memacu pertumbuhan tanaman pisang. Sebanyak enam isolat BPS diperoleh dari tanah rizosfer pisang abaka sehat, tiga di antaranya memiliki aktivitas antagonis terhadap F. oxysporum f. sp. cubense TR4. Empat perlakuan yang dicobakan menunjukkan keparahan penyakit layu fusarium yang tidak berbeda. Demikian juga keempat perlakuan tidak menunjukkan perbedaan pada peubah pertumbuhan yang diamati. Bakteri pelarut silika dan pupuk silika belum mampu mengendalikan F. oxysporum f. sp. cubense TR4. Oleh karena itu, pisang abaka terbukti rentan terhadap F. oxysporum f. sp. cubense TR4.

Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants.

Sweet pepper (Capsicum annuum L.), also known as bell pepper, is one of the most widely grown vegetable crops worldwide. It is attacked by numerous phytopathogenic fungi, such as Fusarium equiseti, the causal agent of Fusarium wilt disease. In the current study, we proposed two benzimidazole derivatives, including 2-(2-hydroxyphenyl)-1-H benzimidazole (HPBI) and its aluminum complex (Al-HPBI complex), as potential control alternatives to F. equiseti. Our findings showed that both compounds demonstrated dose-dependent antifungal activity against F. equiseti in vitro and significantly suppressed disease development in pepper plants under greenhouse conditions. According to in silico analysis, the F. equiseti genome possesses a predicted Sterol 24-C-methyltransferase (FeEGR6) protein that shares a high degree of homology with EGR6 from F. oxysporum (FoEGR6). It is worth mentioning that molecular docking analysis confirmed that both compounds can interact with FeEGR6 from F. equiseti as well as FoEGR6 from F. oxysporum. Moreover, root application of HPBI and its aluminum complex significantly enhanced the enzymatic activities of guaiacol-dependent peroxidases (POX), polyphenol oxidase (PPO), and upregulated four antioxidant-related enzymes, including superoxide dismutase [Cu-Zn] (CaSOD-Cu), L-ascorbate peroxidase 1, cytosolic (CaAPX), glutathione reductase, chloroplastic (CaGR), and monodehydroascorbate reductase (CaMDHAR). Additionally, both benzimidazole derivatives induced the accumulation of total soluble phenolics and total soluble flavonoids. Collectively, these findings suggest that the application of HPBI and Al-HPBI complex induce both enzymatic and nonenzymatic antioxidant defense machinery.

Effective Applications of Trichoderma spp. as Biofertilizers and Biocontrol Agents Mitigate Tomato Fusarium Wilt Disease

Eco-friendly and sustainable plant disease management employing Trichoderma spp. as bioagents is an economically feasible and ecologically sustainable approach. Therefore, their use in agriculture should be encouraged. The two main goals of the present study were to evaluate the abilities of two Trichoderma isolates to prevent Fusarium wilt disease, which is caused by Fusarium solani, in vitro and under greenhouse conditions, as well as their potential as biofertilizers to enhance cherry tomato growth and development. The results of a dual culture test revealed that T. viride and T. harzianum are antagonistic against the F. solani pathogen. The antagonism mechanisms include competition for nutrients and space, mycoparasitism, and antibiosis, according to scanning electron microscopy (SEM) findings. Additionally, T. harzianum reduced the mycelial growth of F. solani by 78.0%, whereas T. viride inhibited the growth by 61.2%, 10 days post-inoculation. In a greenhouse experiment, cherry tomato plants treated with each of these antagonistic Trichoderma isolates separately or in combination significantly suppressed Fusarium wilt disease, improved plant growth parameters, increased macro- and micronutrients uptake, and increased the content of photosynthetic pigments and total phenols. In conclusion, effective applications of Trichoderma isolates have the potential to mitigate Fusarium wilt disease, which is caused by F. solani in cherry tomato plants, while simultaneously promoting the growth and development of cherry tomatoes.

Ferric Oxide Colloid: Towards Green Nano-Fertilizer for Tomato Plant with Enhanced Vegetative Growth and Immune Response Against Fusarium Wilt Disease

Global food crisis due to climate change, pandemic COVID-19 outbreak, and Russia-Ukraine conflict leads to catastrophic consequences; almost 10 percent of the world’s population go to bed hungry daily. Narrative solution for green agriculture with high vegetation and crop yield is mandatory; novel nanomaterials can improve plant immunity and restrain plant diseases. Iron is fundamental nutrient element; it plays vital role in enzyme activity and RNA synthesis; furthermore it is involved in photosynthesis electron-transfer chains. This study reports on the facile synthesis of colloidal ferric oxide nanoparticles as novel nano-fertilizer to promote vegetation and to suppress Fusarium wilt disease in tomato plant. Disease index, protection percent, photosynthetic pigments, and metabolic indicators of resistance in plant as response to induction of systemic resistance (SR) were recorded. Results illustrated that Fe2O3 NPs had antifungal activity against F. oxysporum. Fe2O3 NPs (at 20 µg/mL) was the best treatment and reduced percent disease indexes by 15.62 and gave highly protection against disease by 82.15% relative to untreated infected plants. Fe2O3 NPs treatments in either (non-infected or infected) plants showed improvements in photosynthetic pigments, osmolytes, and antioxidant enzymes activity. The beneficial effects of the synthesized Fe2O3 NPs were extended to increase not only photosynthetic pigments, osmolytes contents but also the activities of peroxidase (POD), polyphenol oxidase (PPO), catalase (CAT) and superoxide dismutase (SOD), enzymes of the healthy and infected tomato plants in comparison with control. For, peroxidase and polyphenol oxidase activities it was found that, application of Fe2O3 NPs (10 µg/mL) on challenged plants offered the best treatments which increased the activities of POD by (34.4%) and PPO by (31.24%). On the other hand, application of Fe2O3 NPs (20 µg/mL) on challenged plants offered the best treatments which increased the activities of CAT by (30.9%), and SOD by (31.33%).

Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings

BackgroundThe promotion of plant growth and suppression of plant disease using beneficial microorganisms is considered an alternative to the application of chemical fertilizers or pesticides in the field.ResultsA coconut-scented antagonistic Trichoderma strain LZ42, previously isolated from Ganoderma lucidum-cultivated soil, was investigated for biostimulatory and biocontrol functions in tomato seedlings. Morphological and phylogenetic analyses suggested that strain LZ42 is closely related to T. atroviride. Tomato seedlings showed increased aerial and root dry weights in greenhouse trials after treatment with T. atroviride LZ42 formulated in talc, indicating the biostimulatory function of this fungus. T. atroviride LZ42 effectively suppressed Fusarium wilt disease in tomato seedlings, with an 82.69% control efficiency, which is similar to that of the carbendazim treatment. The volatile organic compounds (VOCs) emitted by T. atroviride LZ42 were found to affect the primary root growth direction and promote the root growth of tomato seedlings in root Y-tube olfactometer assays. The fungal VOCs from T. atroviride LZ42 were observed to significantly inhibit F. oxysporum in a sandwiched Petri dish assay. SPME–GC–MS analysis revealed several VOCs emitted by T. atroviride LZ42; the dominant compound was tentatively identified as 6-pentyl-2H-pyran-2-one (6-PP). The VOC 6-PP exhibited a stronger ability to influence the direction of the primary roots of tomato seedlings but not the length of the primary roots. The inhibitory effect of 6-PP on F. oxysporum was the highest among the tested pure VOCs, showing a 50% effective concentration (EC50) of 5.76 μL mL−1 headspace.ConclusionsTrichoderma atroviride LZ42, which emits VOCs with multiple functions, is a promising agent for the biostimulation of vegetable plants and integrated management of Fusarium wilt disease.

Potential of biosynthesized zinc oxide nanoparticles to control Fusarium wilt disease in eggplant (Solanum melongena) and promote plant growth

In this study, a novel, non-toxic, eco-friendly zinc oxide nanoparticles (ZnO-NPs) was used instead of the synthetic fungicides widely used to control the destructive phytopathogenic fungus Fusarium oxysporum, the causative agent of wilt disease in Solanum melongena L. Herein, the biosynthesized ZnO-NPs was carried out by Penicillium expansum ATCC 7861. In vitro, mycosynthesized ZnO-NPs exhibited antifungal activity against Fusarium oxysporum. In vivo, ZnO-NPs suppressed Fusarium wilt disease in cultivated Solanum melongena L. by decreasing the disease severity with 75% of plant protection. Moreover, ZnO-NPs stimulated the recovery of eggplant as an indicated by improving of morphological and metabolic indicators including plant height(152.5%), root length(106.6%), plant fresh biomass (146%), chlorophyll a (102.8%), chlorophyll b (67.86%), total soluble carbohydrates (48.5%), total soluble protein (81.8%), phenol (10.5%), antioxidant activity and isozymes compared with infected control. Therefore, this study suggests using mycosynthesized ZnO-NPs as an alternative to synthetic fungicides not only to eradicate the Fusarium wilt disease in cultivated eggplant (Solanum melongena) but also to promote the growth parameters and metabolic aspects.

Iron-Carbon Nanofibers Coated with Acylated Homoserine Lactone Enhance Plant Growth and Suppress Fusarium Wilt Disease in Cicer arietinum by Modulating Soil Microbiome

Iron-Carbon Nanofibers Coated with Acylated Homoserine Lactone Enhance Plant Growth and Suppress Fusarium Wilt Disease in <i>Cicer arietinum</i> by Modulating Soil Microbiome

Humic substances suppress Fusarium oxysporum by regulating soil microbial community in the rhizosphere of cucumber (Cucumis sativus L.)

Fusarium wilt causes severe yield loss in greenhouse cucumbers. Humic substances refer to organic amendments typically applied in the soil to suppress pathogens and promote vegetable growth. The present study evaluated the effects of two humic substances (humic acid (HA)) from weathered coal and sodium humate from peat (HN) on the relative abundance of Fusarium oxysporum (F. oxysporum) and the greenhouse cucumber growth. HA and HN showed different chemical structures as per the Fourier transform infrared spectroscopy. HA and HN had different impacts on soil bacterial and fungal community compositions as well as on their network topological patterns. Specifically, both HA and HN increased the relative abundance of Firmicutes, Planctomycetes and Chloroflexi. HA increased the bacterial chao1 index and the observed_OTUs, while HN improved both the bacterial and fungal alpha-diversities. Both HA and the HN also increased the network interactions, as well as the clustering coefficients (21.2%–21.5%), degrees (387%–554%) and densities (300%–467%) compared to the unamended control. The structural equation model indicated that the HA and HN demonstrate positive correlations with the bacterial and fungal community structure and density; besides, the fungal community structure was negatively correlated with network density. The latter showed a negative correlation with the disease index. Hence, HA and the HN increase the fungal network complexity of the rhizosphere soil and downgrade the network density and subsequently the disease index; as a result, the aboveground biomass of the greenhouse cucumbers increases. Overall, this study demonstrates the link between humic substances and microbial community structures and highlights the significance of network topological patterns in suppressing Fusarium wilt disease and promoting cucumber growth.

Biodiversity of Trichoderma antagonist saprophytic fungi and its use for biocontrol of Fusarium wilt disease on shallots at Lombok Island, West Nusa Tenggara, Indonesia

One of the obstacles in the development of shallots in West Nusa Tenggara (NTB) is the presence of Fusarium wilt disease. The use of antagonistic saprophytic fungi Trichoderma spp. is a biological control technique. The aim of this research is to explore the biodiversity of Trichoderma spp. and its use for controlling Fusarium wilt disease. Research using exploratory methods conducted at the shallot planting center on Lombok Island included isolation of antagonistic saprophytic fungi, purification and identification of isolates. Furthermore, research was carried out in the laboratory using experimental methods including in-vitro antagonism tests by direct opposition and culture steam. The research was continued in Greenhouses in planta and in field conditions in the Highlands of Sembalun Village, Medium Plains of Santong Village and Senteluk Village Lowlands. The results concluded that: there were 6 isolates of saprophytic fungi that were antagonistic, namely T. viride, T. longibrachiatum, T. koningii, T. piluliferum, T. harzianum, and T. hamatum. The mechanism of antagonism is physically through space competition, mycoparasites and antibiosis. Three isolates, namely T. virede, T. harzianum and T. hamatum, were effective in suppressing Fusarium wilt disease and even causing immunity.

Trichoderma biofungicides formulations on shallot growth, yield and fusarium wilt disease resistance

The impact of climate change is an increase in extreme climatic events that make plants susceptible to disease. Fusarium wilt disease is one of the endemic diseases in extreme climatic areas in the high and lowlands. To deal with this climate change adaptation, a study was carried out that aims to determine the effect of the biofungicide Trichoderma spp. in controlling Fusarium wilt disease in several shallot varieties. This study used an experimental method carried out in the Highlands of Sembalun Bumbung Village, East Lombok using a Randomized Block Design with factorial experiments. The main factor is the formulation of biofungicides, namely: without biofungicides (control), liquid biofungicides, tablets and powders. The simple factor is shallot varieties, namely: Bali Karet, Ampenan, Keta Monca, Super Philip, and Super Cross. The results showed that liquid, tablet and powder of biofungicide could suppress Fusarium wilt disease up to 0%, while in control the percentage of disease reached 60%. Bali Karet varieties are very adaptive in the highlands, so they are very resistant to Fusarium wilt, Ampenan and Keta Monca adaptive in the highlands, while Super Philip and Super Cross are less adaptive. Bali Karet varieties showed the highest growth and yield.

Selection of Rhizosphere Bacterial Isolates and Development of Antagonistic Bacterial-Based Formulation to Control Fusarium Wilt on Shallot

&lt;p&gt;Wilt disease caused by Fusarium spp. is a major disease in shallot-producing areas particularly in the North Coast of West Java. The research was aimed to select rhizosphere bacteria and develop an antagonistic bacterial-based formulation for its effectiveness to suppress wilt disease caused by Fusarium spp. on shallot plant under greenhouse and field trials. The field trials were carried out using randomized complete block design with four replications at the shallot farmer condition in Subang and Indramayu during the 2019 planting season. Antagonistic test of bacterial isolates from the rhizosphere showed that most of the bacterial isolates can be used as a biocontrol to Fusarium spp. pathogen. The result revealed that bacterial suspension formulation (E-76 + DBS-2 isolates) in combination with phytohormone (1:1 [v/v]) at Subang and Indramayu tended to suppress Fusarium wilt disease intensity with the disease inhibition ranging from 73.54–93.39% and 66.3–95.65%, respectively. The spraying application of formulation 2.5 ml/l was obtained as the best formula to suppress the disease. The growth and production of shallot were also affected by the application of antagonistic-bacterial formula.&lt;/p&gt;

A phytoremediation coupled with agro-production mode suppresses Fusarium wilt disease and alleviates cadmium phytotoxicity of cucumber (Cucumis sativus L.) in continuous cropping greenhouse soil

Cadmium (Cd) contamination and continuous cropping obstacle often coexist in greenhouse soil and seriously restrict cucumber production. In this study, hyperaccumulator Sedum alfredii Hance was intercropped with spring cucumber (Cucumis sativus L.), then rotated with low accumulator water spinach and autumn cucumber under rational water regime, composited amendment was applied to soil before transplanting autumn cucumber. The results showed that, compared with conventional crop rotation system (Chinese cabbage and cucumber rotation), superposition management practice suppressed Fusarium wilt disease by 28.4 and 57.4% and increased yield by 35.2 and 383% for spring and autumn cucumbers, respectively. Meanwhile, photosynthetic characteristics, antioxidant system and fruit quality were significantly improved. Furthermore, this mode modified soil microbial community structure, enhanced soil enzyme activities, and simultaneously reduced soil total and phytoavailable Cd by 30.3 and 47.7%, respectively. These results demonstrated a feasible technical system to achieve phytoremediation coupled with argo-production in Cd contaminated greenhouse soil with continuous cropping obstacle and provided useful information for further revelation of interaction mechanisms between multicropping and comprehensive biofortification measurements.