Stress is a biological response in the form of physiological, biochemical, hematological, and behavioral changes to internal or external stimuli that threaten the homeostasis of living beings. Effects that activate the defense system in living things are defined as stressors, and it is possible to talk about many different stress factors. Factors that cause stress can be divided into environmental, physical, social or emotional. Stress reactions, which begin with the effect of the stressor, vary according to the duration and severity of exposure to stress. In animal welfare, stress has many negative effects on organism. These negative effects may cause many problems and, shape future by adding problems such as stress and chain links in animals. Just as well-being is for humans, well-being is very important for animals. To determination of glucocorticoids or metabolites in the bloodstream of an organism under stress, noninvasive methods that provide reliable stress measurement without interfering with the organism have recently become increasingly popular. In this review article study, nineteen articles from various parts of the world were examined. In this review, measures of stress by non-invasive methods by looking at GlucoCorticoid Metabolites (GCM) and the latest developments in this field are discussed. In this review article study, nineteen articles from various parts of the world were examined. As a result of the articles reviewed, non-invasive methods for measuring stress may aid and improve our understanding of stress biology and animal welfare. Applying this method to many animal species and biological materials will provide accurate results and support animal welfare.