PurposeThe purpose of this study is to identify and analyze the barriers associated with the adoption of Industry 4.0 technologies in agricultural supply chains.Design/methodology/approachThe study initially identified thirteen barriers by conducting a literature review and semi-structured interviews with key stakeholders. Subsequently, these barriers were validated and modeled using an integrated Fuzzy Delphi-ISM approach. Finally, MICMAC analysis was employed to categorize the barriers into distinct clusters.FindingsThe results provide considerable insights into the hierarchical structure and complex interrelationships between the barriers as well the driving and dependence power of barriers. Lack of information about technologies and lack of compatibility with traditional methods emerged as the two main barriers which directly and indirectly influence the other ones.Research limitations/implicationsThe robust hybrid Fuzzy Delphi and ISM techniques used in this study can serve as a useful model and benchmark for similar studies probing the barriers to Industry 4.0 adoption. From a theoretical standpoint, this study expands the scope of institutional theory in explaining Industry 4.0 adoption barriers.Practical implicationsThe study is timely for the post-COVID-19 recovery and growth of the agricultural sector. The findings are helpful for policymakers and agriculture supply chain stakeholders in devising new strategies and policy interventions to prioritize and address Industry 4.0 adoption barriers.Originality/valueIt is the first comprehensive, multi-country and multi-method empirical study to comprehensively identify and model barriers to Industry 4.0 adoption in agricultural supply chains in emerging economies.