Multi-enzyme Supplementation Research Articles

PSIV-4 Effect of combinations of feed enzymes on growth performance, and nutrient digestibility in nursery pigs fed complex diets

Abstract Feed enzymes provide opportunities to enhance nutrient utilization and intestinal health of newly weaned pigs. The objective of this study was to evaluate supplemental effects of multi-enzymes in diets that are 7% deficient in SID Lys and other essential amino acids. Sixty four pigs weaned at 7.22 ± 0.04 kg BW were allotted to 2 dietary treatments with 8 replicates per treatment and 4 pigs per pen based on a completely randomized block design using sex and initial BW as blocks. Dietary treatment was the supplementation of multi-enzymes (4,000 U of xylanase, 150 U of amylase, 150 of β-glucanase, and 3,000 of U protease/kg, DuPont Animal Nutrition, UK). Pigs were fed based on 2 phases (14 and 27 days for each phase). Phase 2 diets included titanium dioxide as an indigestible external marker to calculate nutrient digestibility. Body weight and feed intake were measured at d 0, 14, and 42. From d 40 to 42, fresh fecal samples were collected from each pen and pooled by pen, weighed, freeze dried, and ground. Diet and fecal samples were used to quantify dry matter, titanium dioxide, and gross energy. Data were analyzed using the MIXED procedure of SAS. Initial BW were not different among treatments. Supplementation of multi-enzymes increased (P < 0.05) ADG (69 to 131 g/d), ADFI (134 to 181 g/d), and G:F (0.411 to 0.717) during phase 1. During phase 2, growth performance was not different among pigs. Supplementation of multi-enzyme increased (P < 0.05) apparent total tract digestibility (ATTD) of gross energy (84.5 to 89.3%). From this study, supplementation of 4,000 U of xylanase, 150 U of β-glucanase, 3,000 U of protease/kg, and 150 U amylase enhanced growth performance of pigs during the first 14 days after weaning and energy digestibility at 42 days after weaning.

Dietary cassava peel meal, methionine, and multi-enzyme supplementation in rabbits\u2019 nutrition: effect on growth, digestibility, and carcass traits

BackgroundThis study was carried out to assess the performance indices, nutrient digestibility, and carcass trait of weaner rabbits fed diets containing cassava peel meal (CPM) supplemented with methionine (MET) and multi-enzyme (ENZ). Two hundred and forty 5-week-old growing rabbits were randomly allotted to 8 dietary treatments (10 replicates/treatment) in a factorial arrangement (2 × 2 × 2) of 2 CPM inclusion levels (0 and 35%), 2 MET levels (0.56 and 0.83%), and 2 ENZ levels (0 and 0.05%).ResultsThe final weight gain (1563.16 g), daily weight gain (17.67 g), and feed conversion ratio of rabbits fed diet 5 (35% CPM; 0.56% MET) were significantly (P < 0.05) lower than those fed the rest of the diets. While the 35% CPM dietary inclusion significantly led to the performance and dry matter digestibility reduction, the high methionine (0.83%) and multi-enzyme (0.05%) significantly (P < 0.05) improved the final weight gain (FWG) and feed and conversion ratio in the rabbits. The interactive effect of methionine and multi-enzyme supplementation on FWG of the rabbits was significant (P < 0.05). Dry matter (DM) digestibility decreased (P < 0.05) with CPM inclusion, while the DM, organic matter (OM), crude protein (CP), acid detergent fiber, and gross energy (GE) digestibility improved (P < 0.05) with high methionine (0.83%) and enzyme supplementation. The slaughter weight (SLW), carcass weight, and carcass weight percentage of rabbits on diet containing 35% CPM and 0.56% methionine but without enzyme supplementation (diet 5) was significantly (P < 0.05) lower than the rest of the diets. There were significant (P < 0.05) increases in liver and kidney weights of rabbits fed diet 5.ConclusionThe growing rabbit’s diet with 35% CPM inclusion will require the supplementation of methionine at a high level in addition to multi-enzyme supplementation to achieve a desired performance by the farmer.

Porcine in vitro digestion and fermentation characteristics of corn wet distillers’ grains and dried distillers grains with solubles without or with multi-enzyme

Wet distillers grains (wet DG) and condensed distillers are co-products from ethanol plants, which are combined and dried into distillers grains with solubles (DDGS). Information is lacking on effects of producing DDGS from wet DG on response to fiber-degrading enzymes in terms of digestibility of DDGS for pigs. This study determined porcine in vitro digestion and fermentation characteristics of wet DG and DDGS without or with multi-enzyme that supplied 9600 U of xylanase, 1200 U of glucanase, 4000 U of cellulase, 480 U of mannanase, 5600 U of invertase, 40,000 U of protease, and 96,000 U of amylase/kilogram of feedstuff. Four gram samples were weighed into conical flasks (5 flasks per treatment) and hydrolyzed in 2 steps using pepsin and pancreatin. Subsequently, undigested residues were incubated in a buffer solution with minerals and fresh pig feces as inoculum. Gas production was measured for 72 h, and modeled to estimate kinetics of gas production. Concentration of volatile fatty acids (VFA) was measured in fermented solutions. On dry matter (DM) basis, the wet DG and DDGS contained 235.2 and 288.7 g/kg crude protein, and 62.5 and 109.9 g/kg ether extract, respectively. There was no interaction between feedstuff and multi-enzyme on any of the response criteria measured in this study. Coefficient of in vitro digestibility of DM (IVDDM) of wet DG (0.497) did not differ from that of DDGS (0.498). Multi-enzyme supplementation did not affect the IVDDM of wet DG or DDGS. Total gas production per unit weight of enzymatically unhydrolyzed residue for wet DG was greater (P<0.05) than that of DDGS (122.8 vs 108.9 mL/g DM). Multi-enzyme supplementation increased (P<0.05) the total gas production per unit weight of enzymatically unhydrolyzed residue for wet DG and DDGS by 4.94 and 10.33%, respectively. There was no effect of feedstuff or multi-enzyme on degradation rate and VFA production of enzymatically unhydrolyzed residue. In conclusion, the wet DG and DDGS were similar in IVDDM; however total gas production for enzymatically unhydrolyzed residue for wet DG was greater than that for DDGS, implying that wet DG is more fermentable than DDGS. The multi-enzyme product used in the current study can improve fermentability of wet DG and DDGS for pigs. Feedstuff and multi-enzyme did not interact on any response criteria measured this study, implying that the drying of wet DG into DDGS did not influence the effect of the multi-enzyme on digestibility of DDGS for pigs.

Dietary multi-enzyme complex improves In Vitro nutrient digestibility and hind gut microbial fermentation of pigs.

This study was conducted in two stages to investigate the potential of multi-enzyme supplementation on the nutrient digestibility, growth performance, and gut microbial composition of pigs. In stage 1, effects of multi-enzyme complex (xylanase, α-amylase, β-glucanase, and protease) supplementation on the ileal and total tract dry matter (DM) digestibility of feed-stuffs were investigated with in vitro two-stage and three-stage enzyme incubation methods. A wide range of feed ingredients, namely, corn meal, wheat meal, soybean meal, fish meal, Oriental herbal extract, Italian rye-grass (IRG) and peanut hull were used as substrates. Supplementation of the multi-enzyme complex increased (P < 0.05) the digestibility of the Oriental herbal extract and corn meal. In stage 2, in vivo animal studies were performed to further investigate the effects of the dietary multi-enzyme complex on the nutrient utilization, growth performance, and fecal microbial composition of pigs. A total of 36 weaned pigs were fed corn- and soybean meal-based diets without (control) and with the multi-enzyme complex (treatment) for 6 weeks. Fecal samples were collected from 12 pigs to analyze the microbial communities by using DNA sequencing and bioinformatics tools. Multi-enzyme supplementation had no effect on apparent digestibility of nutrients and growth performance of pigs compared to control. Taxonomic analysis of the fecal samples indicated that the bacteria in both control and treatment samples predominantly belonged to Firmicutes and Bacteroidetes. In addition, the proportion of the phylum Firmicutes was slightly higher in the treatment group. At the genus level, the abundance of Treponema and Barnesiella increased in the treatment group; whereas the numbers ofthe genera including Prevotella, Butyricicoccus, Ruminococcus and Succinivibrio decreased in the treatment group. These results suggest that multi-enzyme supplementation with basal diets have the potential to improve nutrient digestibility and modify microbial communities in the hind-gut of pigs.

Nutrient digestibility of multi-enzyme supplemented low-energy and AA diets for grower pigs1.

A study was conducted to determine effects of supplementing multi-enzyme on apparent ileal digestibility (AID) of energy and AA; and apparent total tract digestibility (ATTD) of energy for pigs fed low-energy and AA diets. Eight ileal-cannulated barrows (initial BW: 38.7 ± 2.75 kg) were fed four diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets were positive control (PC) diet, negative control (NC) diet without or with multi-enzyme at 0.5 or 1.0 g/kg. The PC diet was formulated to meet or exceed NRC (2012) nutrient recommendations for grower pigs (25 to 50 kg), except for Ca and digestible P, which were lower than NRC (2012) recommendations by 0.13 and 0.17 percentage points, respectively, due to phytase supplementation at 1,000 FTU/kg. The NC diet was formulated to be lower in NE by 75 kcal/kg and standardized ileal digestible AA content by a mean of 3%. These reductions were achieved by partial replacement of corn and soybean meal (SBM) and complete replacement of soybean oil and monocalcium phosphate in PC diet with 25% corn distillers dried grains with solubles (DDGS) and 3.6% soybean hulls. Multi-enzyme at 1.0 g/kg supplied 1,900 U of xylanase, 300 U of β-glucanase, 1,300 U of cellulase, 11,500 U of amylase, 120 U of mannanase, 850 U of pectinase, 6,000 U of protease, and 700 U of invertase per kilogram of diet. The AID of GE, N, most AA, most component sugars of nonstarch polysaccharides (NSP) and P; ATTD of GE for PC diet was greater (P < 0.05) than those for NC diets. An increase in dietary level of multi-enzyme from 0 to 1.0 g/kg resulted in a linear increase (P < 0.05) in AID of Ile by 4.3%, and tended to linearly increase (P < 0.10) AID of Leu, Met, Phe, and Val by a mean of 3.4%. Increasing dietary multi-enzyme from 0 to 1.0 g/kg linearly increased (P < 0.05) AID of total NSP and P by 53.7% and 19.2%, respectively; ATTD of GE by 8.4% and DE and NE values by 8.8% and 8.2%, respectively; tended to linearly increase (P < 0.10) AID of GE by 8.1%. The NE values for NC diet with multi-enzyme at 1.0 g/kg tended to be greater (P < 0.10) than that for PC diet (2,337 vs. 2,222 kcal/kg of DM). In conclusion, multi-enzyme supplementation improved energy and nutrient digestibilities of a corn-SBM-corn DDGS-based diet, implying that the multi-enzyme fed in the current study can be used to enhance energy and nutrient utilization of low-energy AA diets for grower pigs.

Effects of multi-enzyme supplementation in a corn and soybean meal-based diet on growth performance, apparent digestibility, blood characteristics, fecal microbes and noxious gas emission in growing pigs

Effects of multi-enzyme supplementation in a corn and soybean meal-based diet on growth performance, apparent digestibility, blood characteristics, fecal microbes and noxious gas emission in growing pigs

Effects of Feeding Graded Levels of Distillers Dried Grains with Soluble DDGS) With or Without Supplementation of Multi-enzymes on Blood Bio-Chemical Constituents of Indigenous Chicken

An experiment was conducted using distillers dried grains with soluble (DDGS) at 0, 10 and 20% levels with or without enzymes supplementation in the diets of indigenous chicken to study the effect on different blood bio-chemicals parameters. A total of one hundred and eighty (21 days old) indigenous chicks were divided into six groups viz. T1, T2, T3, T4, T5 and T6 containing 30 chicks in each group. The birds of T1 (control) and T2 groups were offered the standard chick, grower and layer feeds as per BIS, 2007 without and with multi-enzymes (Xzyme), respectively. The birds of T3, T4, T5 and T6 groups were fed the rations containing 10% DDGS without and with enzymes and 20% DDGS without and with enzymes, respectively. The feeding trial was conducted for a period of 13 fortnights. The average levels of different blood parameters, estimated at the end of the experiment, viz. haemoglobin, glucose, serum protein, lipid, cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium and phosphorus ranged from 9.96±0.20 to 10.91±0.24 g/dl, 185.08±0.54 to 188.67±1.86 mg/dl, 3.05±0.17 to 3.32±0.09 g/dl, 72.45±0.29 to 72.96±0.49 mg/dl, 130.91±0.59 to 133.71±2.07 mg/dl, 71.66±0.44 to 72.28±0.40 U/L, 11.42±0.32 to 11.93±0.12 U/L, 10.16±0.44 to 10.81±0.29 mg/dl and 4.93±0.11 to 5.94±0.14 mg/dl, respectively. Non-significant (P>0.05) differences were observed with respect to average values of different blood parameters among the treatment groups except for blood inorganic phosphorus. Serum concentration of inorganic phosphorus was significantly (P<0.05) lower in T1 as compared to other groups. It can be concluded that the incorporation of DDGS up to 20% level in the diets of indigenous chicken did not have any adverse effect on the blood bio-chemical constituents of experimental birds.

Growth performance, gastrointestinal weight, microbial metabolites and apparent retention of components in broiler chickens fed up to 11% rice bran in a corn-soybean meal diet without or with a multi-enzyme supplement.

We investigated the effects of adding up to 11% rice bran (RB) in corn-soybean meal diets fed to broiler chickens without or with a multi-enzyme supplement (MES). The MES supplied xylanase, β-glucanase, invertase, protease, cellulase, α-amylase and mannanase with targeted activity of 2,500, 300, 700, 10,000, 1,200, 24,000, and 20 U/kg of feed, respectively. The study used a two-phase feeding program (starter, d 0 to 24; finisher, d 25 to 35) with RB added at 5% and 11%, respectively creating 4 diets in each phase. Diets were iso-caloric and iso-nitrogenous and contained phytase (500 FTU/kg) and TiO2 as a digestibility marker. Three hundred and sixty d-old male Ross 708 broiler chicks were placed in cages based on BW (15 birds/cage) and allocated to 4 diets (n = 6). Birds had free access to feed and water. Body weight and feed intake were recorded. Excreta samples were collected 3 d prior to the end of each phase for apparent retention (AR) of components. Samples of birds were sacrificed on d 24 and 35 for gut weight and ceca digesta for organic acid content. There was no interaction (P > 0.10) between RB and MES on BWG and FCR in starter or finisher phase. In finisher phase, birds fed MES had better BWG (961 versus 858 g) and FCR (1.69 versus 1.86) than birds fed non-MES diets (P < 0.01). Feeding RB reduced (P = 0.02) BWG in finisher phase resulting in lower d 35 BW. Birds fed RB had higher (P ≤ 0.01) gizzard weight on d 24 and 35 than non-RB birds. An interaction (P ≤ 0.01) between RB and MES on concentrations of propionic and iso-butyric acids in ceca digesta showed that MES reduced these acids in non-RB diet. The AR of gross energy was higher (P < 0.02) for MES versus non-MES birds in starter and finisher phases. In conclusion, independently, RB increased gizzard weight and reduced final BW whereas MES improved growth and energy utilization.

Les lapins nourris avec de l’amidon de manioc fermenté résidus II: enzyme supplementation

La probabilité d’augmenter le niveau de remplacement optimale du maïs avec de l’amidon de manioc fermenté d’origine microbienne (résidus MFCSR) avec multi-enzyme supplementation en régimes de lapin a été évalué dans un arrangement factoriel 2x3 à huit semaines au procès. Bien que le gain de poids total (GTT) et le ratio de conversion d’alimentation (FCR) a significativement (P

Gliricidia leaf meal and multi-enzyme in rabbits diet: effect on performance, blood indices, serum metabolites and antioxidant status

BackgroundFollowing the ban on the importation of import-dependent fed ingredients in most developing countries, the need to look inward for local content is now compelling. Thus, leaf meals that have phytogenic additive potentials are envisaged will be a viable feed ingredient in rabbit diets.MethodsThe effect of dietary inclusion of gliricidia leaf meal (GLM) with or without multi-enzyme (E) supplementation in rabbits was investigated using ninety-six 35-day old rabbits of crossbreed (Newzealand and Chinchilla). One basal diet that met the requirements of growing rabbit was formulated (Diet 1). Thereafter, another two diets were formulated to contain 15% GLM and 15% GLM plus multi-enzyme at 1 g/kg and designated as diets 2 and 3 respectively. The rabbits were randomly distributed into the 3 diets (32 rabbits/treatment; 4 rabbits/replicate) and fed their respective experimental diets for 8 weeks.ResultsThe body weight and daily weight gain of the rabbits fed on GLM free diet and those on GLM-based diets (diets 1 and 2) were similar at finishing period of 63–91 day but have lower (P < 0.01) values than those rabbits fed GLM + E based diet (diet 3) at finishing period (63–91 days) and whole fattening period (35-91 days). The apparent dry matter and crude protein digestibility of rabbits fed control diet and those fed 15% GLM based diet were lower (P < 0.05) than those fed 15% GLM + E-based diet. Triglycerides concentration of rabbits fed 15% GLM-based diet without enzyme addition were lower (P < 0.05) than those observed for rabbits on the rest test diets. Cholesterol and Low-Density Lipoprotein levels of rabbits fed 15% GLM and 15% GLM + E-based diets were lower (P < 0.05) than those fed the GLM free diet. The superoxide dismutase and glutathione peroxidase of rabbits fed the GLM free diet (diet 1) were significantly (P < 0.05) lower than those fed the 15%GLM and 15% GLM + E-based diets.ConclusionDietary inclusion of GLM at 15% of the diet did not have a negative effect on the rabbits postweaning period (35–63 days) but will require multi-enzyme supplementation to enhance growth indices at finishing period (63–91 day) without precipitating negative effect on the rabbits’ health status.

Effect of pawpaw leaf meal and multi-enzyme supplementation in the diet on performance, digestibility, and oxidative enzyme status of rabbits

BackgroundThis study aimed at determining the effect of pawpaw leaf meal (PLM 26.14% crude protein [CP] and 16.06% crude fiber [CF]) and multi-enzyme (m-E: amylase, beta-glucanase, phytase, cellulase, and xylanase, protease) supplementation in diets on performance, nutrient digestibility, and oxidative status of rabbits. Four experimental diets were formulated and designated as diet 1(0%PLM-m-E), diet 2(0%PLM + m-E), diet 3(10%PLM-m-E), and diet 4(10%PLM + m-E). One hundred and twenty (120), 5-week-old rabbits of cross breed rabbits (Chinchilla x New-Zealand white) of equal sexes; weighing 738.21 ± 33 g were randomly distributed to 4 dietary treatments (30 rabbits/treatment; 3 rabbits/replicate).ResultsHighest weight gain being recorded in rabbits fed diet 4 is similar to the weight gain recorded for rabbits fed diets 2 and 3 but higher (P < 0.05) than weight gain of rabbits fed diet 1. Best feed conversion ratio (FCR) recorded in rabbits fed diet 4 was comparable to the FCR of rabbits fed diets 2 and 3 but higher (P < 0.05) than FCR of rabbits fed diet 1. Higher CP digestibility recorded in rabbits fed diets 2 and 4 were similar to CP digestibility in rabbits fed diet 3 but higher (P = 0.01) than CP digestibility in rabbits fed with diet 1. The starch digestibility was higher (P < 0.05) in the rabbits fed m-E supplemented diets 2 and 4. Higher (P < 0.05) glutathione peroxidase and superoxide dismutase activities were recorded in rabbits fed with diets 3 and 4.ConclusionPLM inclusion and m-E supplementation improved performance, crude protein, and starch digestibility and antioxidant enzyme status of the rabbits.

Porcine in vitro degradation and fermentation characteristics of canola co-products without or with fiber-degrading enzymes

An in vitro study was conducted to determine the effects of supplementing solvent-extracted canola meal (SECM) and cold-pressed canola cake (CPCC) with fiber-degrading enzymes (multi-enzyme) on porcine in vitro digestion and fermentation characteristics. Canola co-products without or with multi-enzyme (Superzyme-CS, Canadian Bio-Systems Inc., Calgary, AB, Canada) that supplied 24,000 U of xylanase, 3000 U of glucanase, 10,000 U of cellulase, 1200 U of mannanase, 14,000 U of invertase, 10,000 U of protease, 24,000 U of amylase and 8,500 U of pectinase/l of incubation medium were digested using porcine pepsin and pancreatin. Undigested residue was subjected to in vitro fermentation for 72 h. Accumulated gas production during microbial fermentation was recorded and modeled to estimate kinetics of gas production. Total volatile fatty acids (VFA) concentration per unit weight of DM of enzymatically unhydrolyzed residue incubated, and per unit weight of DM feedstuff that was enzymatically hydrolyzed and then fermented was also measured. On a DM basis, CPCC and SECM contained 318 and 451 g/kg CP, respectively. The in vitro digestibility of DM (IVDDM) for CPCC was greater (P < 0.05) than that for SECM (0.635 vs. 0.584, respectively). Multi-enzyme supplementation tended to improve (P = 0.060) the IVDDM regardless of canola co-product type. Total gas production for CPCC was less (P<0.05) than that for SECM (89.3 vs. 106.0 ml/g, respectively). Multi-enzyme reduced (P < 0.05) total gas production for CPCC and SECM. Total VFA production for CPCC was lower (P < 0.05) than that for SECM (2.89 vs. 4.43 mmol/g DM of enzymatically unhydrolyzed residue, respectively). Total VFA production for CPCC was also lower (P < 0.05) than that for SECM (1.07 vs. 1.82 mmol/g DM of feedstuff, respectively). Multi-enzyme did not affect total VFA concentration per unit weight of enzymatically unhydrolyzed for canola co-products but tended to reduce (P = 0.058) total VFA production per unit weight of feedstuff. In conclusion, CPCC was more digestible but less fermentable than SECM. Multi-enzyme supplementation improved the IVDDM and reduced total gas and VFA production for CPCC and SECM. Thus, multi-enzyme shifted the digestibility of CPCC and SECM from microbial fermentation towards enzymatic digestion, implying that the multi-enzyme improved efficiency of utilization of nutrients in the two canola co-products.

279 Amino Acid Digestibility of a Modified Corn Byproduct (Gold Pro) with and with a Multi-Enzyme Supplement (CORE) When Fed to Weanling Pigs.

279 Amino Acid Digestibility of a Modified Corn Byproduct (Gold Pro) with and with a Multi-Enzyme Supplement (CORE) When Fed to Weanling Pigs.

Effects of Eco-friendly Multi-enzyme on Growth Performance, Intestinal Morphology, and Nutrient Digestibility of weaned Pigs

This experiment was conducted to investigate the effects of multi-enzyme on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs (5.92 ± 0.48 kg BW; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multi-enzyme (Multi; mixture of β- mannanase, xylanase, α-amylase, protease, β-glucanase, and pectinase). Pigs were fed their respective diets for 6 wk. Measurements were growth performance, morphology of ileum, apparent ileal digestibility and apparent total tract digestibility of dry matter, crude protein, and energy of weaned pigs. There were no significant differences on growth performance during overall experimental period. No differences were found for the morphology of ileum and nutrient digestibility between CON and Multi groups. Therefore, the results in the current study indicated that multi-enzyme supplementation in diets had no effects on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

Nutritive value of multienzyme supplemented cold-pressed camelina cake for pigs.

Cold-pressed camelina cake (CPCC) is a fibrous co-product of camelina seed pressing and available for livestock feeding. However, information is lacking on the effect of supplementing fiber-degrading enzymes to CPCC-based diets on nutrient utilization by pigs. Experiment 1 determined the effect of multienzyme supplementation on standardized ileal digestibility (SID) of amino acid (AA) and net energy (NE) value of CPCC for pigs. Six ileal-cannulated barrows (average initial body weight [BW] = 36 kg) were fed five diets in 5 × 5 Latin square design with 1 added column to give six replicates per diet. The diets were a corn-soybean meal (SBM)-soybean oil-based diet and the basal diet with corn, SBM, and soybean oil replaced by 25% CPCC with or without multienzyme (600 U of xylanase, 75 U of glucanase, 250 U of cellulose, 30 U of mannanase, 350 U of invertase, 2,500 U of protease, and 6,000 U of amylase/kg of diet; Superzyme-CS, 0.5 g/kg) in a 2 × 2 factorial arrangement. The fifth diet was a low-casein cornstarch-based diet. The ratio of corn to SBM and soybean oil in the basal diet was identical to the CCPC-containing diets to allow calculation of nutrient digestibility of CPCC by the difference method. On a dry matter (DM) basis, CPCC contained 42% crude protein, 10.5% ether extract, 25.4% neutral detergent fiber (NDF), 2.07% Lys, 0.73% Met, 1.64% Thr, 0.51% Trp, and 22.1 trypsin inhibitor units per milligram, respectively. The SID of Lys, Met, Thr, and Trp for CPCC were 43.5%, 70.7%, 44.8%, and 55.3%, respectively. The digestible energy (DE) and NE values for CPCC were 3,663 and 2,209 kcal/kg of DM, respectively. Multienzyme supplementation did not affect the SID of AA, and DE and NE values for the corn-SBM-CPCC-based diet, and for the CPCC. In experiment 2, the effects of multienzyme dosage (0.5 or 50 g/kg of treated feedstuff) on porcine in vitro digestibility of DM (IVDDM) of CPCC was determined. The IVDDM of CPCC was increased (P < 0.001) with an increase in multienzyme dosage. Multienzyme at 0.5 g/kg did not affect IVDDM of CPCC. However, multienzyme at 50 g/kg increased (P < 0.01) IVDDM for CPCC by at least 16%. In conclusion, multienzyme at 0.5 g/kg did not affect SID of AA and NE values, and IVDDM for CPCC. However, multienzyme at 50 g/kg improved IVDDM of CPCC, implying that the efficacy of the multienzyme with regard to improving nutrient digestibility of CPCC in pigs is dosage dependent.

Influence of exogenous multi-enzymes in broiler chickens fed on maize-wheat-soybean meal-based diets

Summary This study evaluated the effects of multi-enzyme (xylanase, mannanase, β-glucanase, cellulase, pectinase, and amylase) supplementation on growth performance, nutrient digestibility, blood characteristics, relative organ weight, and breast muscle quality in broilers fed maize-wheat-soybean meal-based diets. A total of 800, one-day-old Ross 308 broiler chicks were randomly assigned to one of 4 treatments according body weight (body weight, 45.3 ±Â 0.72 g) in a 35-day trail. Each treatment had 10 replicate cages with 20 birds per replicate cage. Dietary treatment groups were: (1) CON, maize-wheat-soybean meal basal diet; 2) ME60, CON + 60 mg/kg multi-enzyme; 3) ME120, CON + 120 mg/kg multi-enzyme; 4) ME180, CON + 180 mg/kg multi-enzyme.

Effect of Multi-enzymes Supplementation in Egg type Chicks Diet with Varying Protein and Energy levels During Hot-dry Summer

Effect of Multi-enzymes Supplementation in Egg type Chicks Diet with Varying Protein and Energy levels During Hot-dry Summer

Effects of Supplementation of Multienzymes in Diets Containing Different Energy Levels on Growth Performance, Nutrient Digestibility, Blood Metabolites, Microbiota and Intestinal Morphology of Broilers

Effects of Supplementation of Multienzymes in Diets Containing Different Energy Levels on Growth Performance, Nutrient Digestibility, Blood Metabolites, Microbiota and Intestinal Morphology of Broilers

Effects of dietary protein levels and multienzyme supplementation on growth performance and markers of gut health of broilers fed a miscellaneous meal based diet

The current study investigated the effects of dietary protein levels and multienzyme supplementation on the growth performance, subclinical necrotic enteritis, intestinal mucosal barrier and microflora of broilers fed diets containing meals of cottonseeds, rapeseeds, peanuts and sesame. A total of 480 one-day-old male Cobb broilers were assigned to a 2×2 factorial arrangement of treatments with 6 replicated pens of 20 birds each. Experimental factors included two dietary protein levels at 205 or 225g/kg, and without or with multienzymes. The multienzymes contained phytase, protease and xylanase at 1000, 2000 and 2000U/kg of feed, respectively. The trial lasted for 21days. The high protein diet increased (P<0.05) small intestinal lesions, duodenal mucin 2 secretion, ileal counts of C. perfringens and E. coli, but decreased (P<0.05) secretary IgA (sIgA), polymeric Ig receptor (pIgR), ileal counts of Lactobacilli and Bifidobacteria. Multienzymes increased (P<0.05) feed intake, body weight gain, pIgR, sIgA, and ileal counts of Lactobacilli and Bifidobacteria and decreased (P<0.05) intestinal lesions, serum a-toxin antibodies, mucin 2 expression and ileal count of C. perfringens, but the magnitude of the effect of multienzymes was dependent on the protein level in the diet (interaction, P<0.05). It can be concluded that diets containing high nonconventional protein can increase the incidence of subclinical necrotic enteritis, while supplementing multienzymes can decline this risk in broilers.

The microbial pH-stable exogenous multienzyme improved growth performance and intestinal morphology of weaned pigs fed a corn–soybean-based diet

ABSTRACTThe present study investigated the effects of dietary supplementation of multienzyme preparation on growth performance, apparent total tract digestibility (ATTD) of nutrients, and gut health of weaned pigs. A total of 240 weaned pigs (initial body weight: 7.3 ± 0.7 kg) were randomly allotted to five treatments on the basis of body weight. The dietary treatments included a corn–soybean meal (SBM)-based diet supplemented with 0 (control), 0.025, 0.050%, 0.075%, or 0.10% multienzyme preparation. The experimental diets were fed in a meal form for two phases (d 0–14, phase I and d 15–28, phase II). Increasing multienzyme supplementation linearly improved overall (d 0–28) average daily gain, gain:feed, and ATTD of dry matter, gross energy, and crude protein. At d 21, pigs fed dietary increasing levels of multienzyme preparation had linearly greater Lactobacillus spp. (ileum and caecum and faeces) and fewer Clostridium spp. (ileum and caecum) and Escherichia coli (ileum and faeces) populations. On d 28, villus height of the jejunum and ileum were linearly increased with dietary increase in multienzyme preparation. In conclusion, this multienzyme has potential to improve the growth performance, ATTD of nutrients, and gut health of weaned pigs fed a corn–SBM-based diet in the absence of antibiotics growth promoters.