Superior Product Research Articles

Novel Cancer Immunotherapy Using Cell-Derived Membrane Vesicles Orchestrates Multimodal Antitumor Immunity

Cancer immunotherapy has emerged as a promising therapy for hematological malignancies. Although multiple immunotherapeutics are currently available in the clinic such as chimeric antigen receptor-engineered T-cell therapy (CAR-T cell therapy) and bispecific T-cell engager (BiTE), their long-term efficacy is still insufficient in most of the cases. Simultaneous inputs of multiple signals may provide more optimal T-cell activation and overcome the immunosuppressive tumor microenvironment (TME), which results in a durable therapeutic effect. However, integrating multifaceted immunomodulatory signals into a single drug or cell is technically difficult. To overcome these limitations, we developed nanosized cell-derived membrane vesicles (MVs) that orchestrate multimodal antigen-specific antitumor immunity in endogenous T cells. We isolated MVs with 100-150 nm in size and intact membrane-bound proteins from the HLA-negative leukemia cell line K562 by mechanical homogenization followed by isolation of the plasma membrane fraction using magnetic beads and ultracentrifugation. MVs can be loaded with a desired combination of immunomodulatory molecules on the surface by preparing cells stably transduced with those proteins. First, K562 cells were genetically engineered with a membrane-bound form of the anti-CD3/CD19 BiTE and homogenized to form BiTE-expressing MVs. When co-cultured with T cells and CD19-positive leukemia cell line NALM-6, MVs loaded with anti-CD3/CD19 BiTE induced potent cytotoxic activity in nonspecific T cells in a dose-dependent manner. To provide optimal T-cell activation, we further loaded MVs with a variety of immunostimulatory molecules including costimulatory ligands (CD80 and 4-1BBL) and cytokines (IL-7 and IL-15) on the surface. The generated antitumor MVs simultaneously induced multiple immunostimulatory signals in T cells, resulting in superior proliferation, cytokine production, and cytolytic functions compared with MVs loaded with BiTE alone. Intriguingly, MVs activated T cells only in the presence of target tumor cells, which is considered to provide a safety advantage in clinical settings. Changing the antitumor mAb sequences of the BiTE enabled to generate MVs against any type of antigen including B cell maturation antigen (BCMA), EGFR, and mesothelin. To test functions of our MVs in vivo, NSG mice were transplanted with NALM-6 and then infused with human peripheral blood T cells. While infusion of T cells alone did not contribute to controlling leukemia progression, infusion of anti-CD19 MVs with CD80, 4-1BBL, IL-7, and Il-15 induced potent antitumor response. Repeated infusion of MVs further augmented their therapeutic efficacy. To overcome the immunosuppressive TME, we additionally loaded MVs with immune checkpoint inhibitors (anti-PD-1 and anti-CTLA-4 antibody), TGF-b receptors to trap TGF-b, and inflammatory cytokines (IL-12 and IL-18) that activate both T cells and myeloid cells in the TME. Immunocompetent Balb/c mice were subcutaneously inoculated with colon carcinoma cell line CT26 ectopically expressed with CD19 and treated with MVs targeting CD19. The injected MVs converted tumor-associated M2-type macrophages into an inflammatory phenotype and promoted CD8+ T cell infiltration within the TME, resulting in better control of tumor progression in in vivo solid tumor mouse models. K562 cells are highly sensitive to NK cells because they endogenously express several ligands for NK cell activation receptors such as MIC-A and MIC-B, and lack the expression of HLA class I that represses NK cell activation. K562-derived MVs induced potent cytotoxic activity in NK cells against NK-cell-resistant myeloma cell line MM1S in an antigen-specific manner. In summary, this novel technology enables to deliver desired combinations of antitumor immune signals to not only T cells but also NK cells and other types of immune cells, which can transmit superior therapeutic efficacy against the tumor cells that are resistant to the present immunotherapeutics.

Age at first calving and productive performance of primiparous Holsteins.

This study investigated the age at first calving in Holstein heifers and its impact on various production parameters. A sample of 737 Holstein heifers born between 2015 and 2018 and finishing their first lactations between 2018 and 2020 was included. Cluster analysis revealed three groups based on age at first calving: high precocity, medium precocity and low precocity. Medium-precocity group exhibited the highest 305-day milk yield and peak milk production. Additionally, the same group demonstrated superior mean production per lactation, 305-day milk protein content, and 305-day milk yield. The lowest somatic cell count was found in the low-precocity group. In conclusion, medium-precocity cows showed better results such as higher 305-day milk yield and peak milk production.

PPPUD Group "Maja Tani Soka" in Banjar Soka, Senganan Village Penebel District, Tabanan District

Regional Featured Product Development Program Activities in Banjar Soka, Senganan Village, Penebel, Tabanan, Bali at the Maja Tani Soka Group, engaged in organic farming, namely producing organic red rice. The availability of rice is not sustainable, partners want to increase production both in quantity and quality, Partners do not yet understand the proper and correct technology for cultivating plants using an organic system; Organic fertilizer has not been fermented properly. Solutions by providing appropriate technology in cultivating rice plants and making organic fertilizer, organic pesticides and packaging. The method is technology transfer and introducing science and technology, namely outreach activities, training, mentoring, demonstration plots; both partners are actively and creatively invited to work together. The result is superior products of Maja liquid organic fertilizer and Maja organic pesticides and also an increase in the production of organic red rice qualitatively and quantitatively and the welfare of partners increases

Optimization of tilapia (Oreochromis niloticus) viscera oil extraction using response surface methodology

Tilapia is one of the export commodities with superior fish fillet products. Fillet processing produces much waste, one of which is viscera, which has the potential as raw material for fish oil. Several factors, including the method, temperature, and extraction time, influenced the quality of fish oil. This study aimed to determine the effect of wet rendering, extraction time and temperature on tilapia viscera oil and determine the best temperature and time based on the characteristics of fish oil using response surface methodology (RSM). The data analysis comprised the peroxide value, free fatty acid, anisidine value, total oxidation, and yield value. The result was then optimized using the response surface method with Design Expert 11. The recommended model is linear. The results for extraction at 60℃ for 25.95 mins had a yield, peroxide, free fatty acid, p-anisidine, and total oxidation value of 21.6%, 6.69 meq/kg, 4.16%, 3.87 meq/kg, 17.79, respectively, with, a desirability value of 0.62.

Analisis Ergonomi di Lingkungan Kerja Industri Rumah Tangga Kerupuk Ikan Tenggiri Agung Rasa di Cilacap

The fish processing industry in Cilacap district is a business that is quite popular, because processed fish is a superior product in Cilacap district, one of which is mackerel fish crackers. This is influenced by the fact that the Cilacap district is a coastal area and is a place for tourist visits so that people's interest in various food flavors is quite high. Ergonomic analysis is needed to create safe, comfortable and healthy working conditions so that work activities can be effective which has an effect on increasing productivity. The method used in this research is descriptive experimental through observation and interview methods. Located in the Agung Rasa mackerel fish cracker home industry. The results of the research obtained an ergonomic analysis using an approach to 8 ergonomic aspects in the form of use of muscle power, work attitude, nutrition, work environment, work time, information systems, socio-cultural conditions and human-machine interaction which is known to still not show ergonomic working conditions. From the results of this analysis, recommendations can be made in the form of implementing short breaks or implementing exercise between work activities to avoid feelings of boredom, fatigue and musculoskeletal complaints, then improvements to work stations, regular working time arrangements and layout arrangements can be implemented to create ergonomic working conditions.

The effects of stance width during barbell hip thrust on power and velocity output among adolescent Silat athletes

Background and Study Aim. In the physically demanding combat sport of Silat, strength and power dominate. Consequently, applying various stance widths during barbell hip thrusts may tailor athletes' lower-body exercises to individual needs. This has the potential to optimize performance. The aim of this study is to investigate the impact on performance of power, speed, and stance width among Silat combat athletes. Material and Methods. Participants performed 10RM tests in three stance widths: wider than shoulder width (WSW), normal shoulder width (NSW), and narrower than shoulder width (NRW). This was done using a 72-hour counterbalance cross-over study design. Power and velocity were measured and analyzed using a mixed ANOVA design. Results. The results indicated a significant main effect of stance width on power (F(2,56) = 3.086, p < 0.05) and velocity (F(2,56) = 3.683, p < 0.03) output. Both males and females demonstrated the highest power in NRW (M = 413.26, SD = 131.76; M = 239.53, SD = 111.16), followed by WSW and NSW. A strong positive correlation between power and velocity was observed for all stance widths: WSW (r(28) = 0.77, p < 0.001), NSW (r(28) = 0.79, p < 0.001), and NRW (r(28) = 0.89, p < 0.001). NRW was associated with superior power production, while WSW facilitated higher velocity. Conclusion. The results of this study demonstrate the importance of considering a variety of stance width techniques during exercise due to their effects on power and velocity during the barbell hip thrust exercise. Coaches can tailor training programs with a velocity-targeted strength and conditioning approach to enhance performance and competitiveness. Further research should investigate different athlete groups and age levels to refine training methodologies.

Enhanced interfacial charge transfer by oxygen vacancies in ZnCdS/NiCo-LDH heterojunction for efficient H2 evolution

The elaborate modulation of charge transfer in Z-scheme heterojunctions is of significant importance yet poses a formidable challenge for achieving efficient photocatalytic hydrogen production. The present study reports the fabrication of ZnCdS nanoparticle wrapped by NiCo-layered double hydroxide with enriched oxygen vacancies (ZCS@LDH-Ov) was fabricated, which exhibits enhanced interfacial binding and internal electric field (IEF) in comparison to ZCS@LDH. Under visible-light irradiation, the as-prepared ZCS@LDH-Ov exhibits a superior photocatalytic H2 production rate of up to 2.68 mmol·g−1·h−1, which is approximately 9.24-fold and 2.68-fold of the pristine ZnCdS and ZCS@LDH, respectively. The stability of the photocatalyst after 5 cycles is obviously enhanced, with ZCS@LDH exhibiting a remaining hydrogen evolution reaction (HER) rate of 52.7 % and ZCS@LDH-Ov demonstrating an impressive remaining rate of 98.2 %. The Z-scheme facilitates rapid charge transfer and efficient separation, thereby enhancing the catalytic activity for HER. The excellent recyclability of ZCS@LDH-Ov is primarily attributed to enhanced photo-corrosion resistance of ZnCdS, which can be ascribed to the efficient transfer of photo-generated holes through a rapid interfacial charge pathway.

The impact of climate change and production technology heterogeneity on China's agricultural total factor productivity and production efficiency

Sustainable agricultural production efficiency is important for global food security, environmental conservation, economic development, human Health, and social equity. However, Climate change has had a significant impact on global agricultural productivity. To this end, investigating climate change's effect on agricultural production efficiency is critical for the food security of any particular country or region, and China is not distinct. Further, the influencing factor of agricultural total factor productivity (technology or technical efficiency) and regional heterogeneity in agricultural production technologies of China are worth exploring for sustainable agricultural growth. To this end, this study employed the DEA-Malmquist Productivity Index to gauge the total factor productivity change (TFPC) in 31 provinces and administrative units of China from 2000 to 2021. Additional inputs of climate factors were added to the estimation process to explore the impact of climate change on TFPC for different periods and regions. The meta-frontier analysis estimates the agriculture production technology gap among nine regions of China. Results revealed that climate factors could overestimate China's average total factor agricultural productivity over the study period. Among 8 out of 9 regions in China witnessed the diverse effects of climate factors; however, it positively impacted agricultural TFPC in the Qinghai Tibet Plateau. Sichuan Basin and surrounding regions performed best, ranked top in China with an average growth rate of 22.3 % in TFPC. Decomposing the TFPC into efficiency and technological change, the study found that the influence of climate on technological change is greater than compared to efficiency change. Northeast China Plain and Sichuan Basin and surrounding regions have superior agriculture production technology with a TGR score 1. Mann-Whitney U and Kruskal-Wallis test proved the statistically significant difference among agricultural productivity scores estimated with and without climate factors and production technology gaps among nine regions of China.

Designing Improvements To The Product Attributes Of Samase Koko Clothes Based On Consumer Preferences Using The Conjoint Analysis Method

Samase is one of the MSMEs from Bandung that was established in 2014. Samase is engaged in fashion, especially men's Muslim clothing. One of its superior products is koko clothes consisting of koko shirts and koko kurta, but the sales of koko clothes have not been able to meet the set target, this is due to product attributes that are not by consumer desires. This study aims to formulate a combination of attributes and the level of attributes on Samase koko shirt products that are considered important by consumers, by compiling recommendations for improving the right koko clothes attributes according to consumer desires and comparing them with existing attributes. The questionnaire in this study was distributed online to consumers who had bought and used koko clothes products from Samase, totaling 108 respondents. The results of the questionnaire were processed using conjoint analysis to determine consumer preferences. Based on calculations using conjoint analysis, it is known that consumer preferences with the highest utility value are, supernova material, long sleeve size, koko shirt model, deep color, basic collar, printing motifs, and price in the range of Rp. 200,000 - Rp. 300,000

Surface-Degenerate Semiconductor Photocatalysis for Efficient Water Splitting without Sacrificial Agents via a Reticular Chemistry Approach.

The production of green hydrogen through photocatalytic water splitting is crucial for a sustainable hydrogen economy and chemical manufacturing. However, current approaches suffer from slow hydrogen production (<70 µmol·gcat-1·h-1) due to the sluggish four-electrons oxygen evolution reaction (OER) and limited catalyst activity. Herein, we achieve efficient photocatalytic water splitting by exploiting a multifunctional interface between a nano-photocatalyst and metal-organic framework (MOF) layer. The functional interface plays two critical roles: (1) enriching electron density directly on photocatalyst surface to promote catalytic activity, and (2) delocalizing photogenerated holes into MOF to enhance OER. Our photocatalytic ensemble boosts hydrogen evolution by ~100-fold over pristine photocatalyst and concurrently produces oxygen at ideal stoichiometric ratio, even without using sacrificial agents. Notably, this unique design attains superior hydrogen production (519 µmol·gcat-1·h-1) and apparent quantum efficiency up to 13-fold and 8-fold better than emerging photocatalytic designs utilizing hole scavengers. Comprehensive investigations underscore the vital role of the interfacial design in generating high-energy photoelectrons on surface-degenerate photocatalyst to thermodynamically drive hydrogen evolution, while leveraging the nanoporous MOF scaffold as an effective photohole sink to enhance OER. Our interfacial approach creates vast opportunities for designing next-generation, multifunctional photocatalytic ensembles using reticular chemistry with diverse energy and environmental applications.

Surface‐Degenerate Semiconductor Photocatalysis for Efficient Water Splitting without Sacrificial Agents via a Reticular Chemistry Approach

AbstractThe production of green hydrogen through photocatalytic water splitting is crucial for a sustainable hydrogen economy and chemical manufacturing. However, current approaches suffer from slow hydrogen production (&lt;70 μmol ⋅ gcat−1 ⋅ h−1) due to the sluggish four‐electrons oxygen evolution reaction (OER) and limited catalyst activity. Herein, we achieve efficient photocatalytic water splitting by exploiting a multifunctional interface between a nano‐photocatalyst and metal–organic framework (MOF) layer. The functional interface plays two critical roles: (1) enriching electron density directly on photocatalyst surface to promote catalytic activity, and (2) delocalizing photogenerated holes into MOF to enhance OER. Our photocatalytic ensemble boosts hydrogen evolution by ≈100‐fold over pristine photocatalyst and concurrently produces oxygen at ideal stoichiometric ratio, even without using sacrificial agents. Notably, this unique design attains superior hydrogen production (519 μmol ⋅ gcat−1 ⋅ h−1) and apparent quantum efficiency up to 13‐fold and 8‐fold better than emerging photocatalytic designs utilizing hole scavengers. Comprehensive investigations underscore the vital role of the interfacial design in generating high‐energy photoelectrons on surface‐degenerate photocatalyst to thermodynamically drive hydrogen evolution, while leveraging the nanoporous MOF scaffold as an effective photohole sink to enhance OER. Our interfacial approach creates vast opportunities for designing next‐generation, multifunctional photocatalytic ensembles using reticular chemistry with diverse energy and environmental applications.

Construction of Z-scheme heterojunction TiO2-ZnO@Oxygen-doped gC3N4 composite for enhancing H2O2 photoproduction and removal of pharmaceutical pollutants under visible light

In this study, the heterojunction photocatalyst of titanium dioxide-zinc oxide@oxygen-doped graphitic carbon nitride (TiO2-ZnO@OCN (TZ@OCN)) was synthesized using a simple and cost-effective hydrothermal self-assembly process. Characterization of the fabricated materials revealed that the incorporation of TiO2 and ZnO as inherent semiconductors along with the oxygen doping has effectively broadened the absorption spectrum of gC3N4, which can promote the electron generation and provide more active sites for O2 and H+ adsorption. It is noteworthy that the mentioned structure modification resulted in a superior H2O2 production rate among gC3N4-based photocatalysts with up to 3.11 mM.g−1 under visible light irradiation. Moreover, a valence band shifting from 1.57 to 2.91 eV upon doping oxygen atoms also elucidated the participation of active species in the photocatalytic degradation of ciprofloxacin (99.7 %, k = 0.030 min−1) and tetracyline (94.6 %, k = 0.017 min−1), in which superoxide radicals (•O2−) were shown to play a major role in removing the antibiotic. Besides, a Z-scheme heterojunction mechanism is additionally suggested, demonstrating the effectiveness of electron-hole pair splitting in the oxidation of antibiotics. Conclusively, the results show a novel and feasible modification pathway to enhance the photocatalytic activities of gC3N4 and other advanced semiconductor catalysts, especially for eliminating antibiotics and generating H2O2 within aqueous media.

Optimizing the Chemical Composition of Brake Shoes According to the Hardness Recommended by the Product Standard.

Optimizing the chemical composition of phosphorus cast iron for the manufacture of brake shoes, which are used in the rolling stock, is based on specific tests, and the results must be within the specified limits of railway standards. This paper presents the processing of data taken from an economic agent producing brake shoes-with chemical composition and hardness values determined through the Brinell method-as well as optimizing these data by obtaining optimal variation intervals. These technological intervals are useful in the case of foundries in order to obtain superior products from a qualitative point of view.

Sosialisasi dan Pelatihan Literasi Digital untuk Meningkatkan Pengetahuan dan Inovasi Produk Unggulan Masyarakat Desa Poleng Kabupaten Sragen

This article discusses community service activities in Poleng Village, Gesi District, and Sragen Regency which focus on socialization and digital literacy training in an effort to increase knowledge and develop community superior product innovations. This service uses a Participatory Action Research (PAR) approach. The aims of this service is to motivate the people of Poleng to optimize their potential and increase innovation in regional superior products. This service answers the challenges of an increasingly sophisticated digital era and realizes the importance of empowering rural communities to explore their own potential and develop regional superior product innovations so as to increase the competitiveness and economic value of community products. Digital literacy socialization and training using the "SiBooky" digital library is the key in providing skills and literacy to the community for community empowerment and sustainable development. This application aims to realize community empowerment and economic independence, as well as expand access to digital technology for all levels of society. enable people to access information flexibly and easily. Through this program, it is hoped that village communities will be better prepared to face the ever-evolving digital era, be able to contribute to regional development in a sustainable manner, realize community empowerment and economic independence, and expand access to digital technology for all levels of society.

Membangun Potensi Kemandirian Ekonomi Santri Pada Pondok Pesantren Mambaul Ulum Mojokerto

Mamba'ul Ulum Mojosari Islamic boarding school is one of the Islamic boarding schools that has economic independence. This Islamic boarding school has several superior products which have extraordinary potential if marketed appropriately. Apart from that, the concept of entrepreneurship in the era of digital transformation 4.0 is also very important as initial capital towards successful entrepreneurship today. The target to be achieved is how the school (in the Islamic boarding school environment) connects and internalizes the teachings of entrepreneurship and the contribution of economic development to the Islamic boarding school environment, through making products made by Mamba'ul Ulum Islamic boarding school students which will then be sold on e-commerce . This research uses a qualitative-descriptive method, the research results illustrate how the principles created as the basis for the entrepreneurial spirit of santri are useful for improving the economy in the Islamic boarding school environment with the aim of developing economic independence among the santri environment, increasing knowledge and understanding of entrepreneurial potential. There are results achieved in making products to be marketed. The use of online marketing media is simple, mentoring is provided by subject teachers to maintain stable abilities and understanding

PELATIHAN PEMBUATAN PERMEN OLEORESIN LADA PUTIH BANGKA (PIPER NINGRUM LINN.) DI PT. IZZAH GLOBALINDO INDONESIA

White pepper is one of the spices that is widely produced in Bangka. White pepper is only used as a spice. So far there have been no innovative products produced from white pepper. This activity aims to (1) Increase knowledge and information about the potential of Bangka white pepper, oleoresin, hard candy and its economic aspects (2) Make Bangka white pepper hard candy as a superior product of PT. Izzah Globalindo Indonesia so that it can increase income for local farming communities. The method used in this activity is training (socialization) which is carried out as part of the Kedaireka activities of Djuanda University aimed at PT Izzah Globalindo Indonesia. The result of this training is to provide knowledge and the creation of Bangka white pepper oleoresin hard candy products, as a product that can become a superior product of PT. Izzah Globalindo Indonesia.

Influence of pretreatment on properties of bamboo portland cement particle board

Bamboo contains water-soluble saccharides and carboxylic acid which have an anticoagulation effect on Portland cement, and the anticoagulation ingredients can directly influence the hydration reaction extent. Hydration product varieties and hydration product-bamboo shaving binding interfaces of the Portland cement, and finally the mechanical properties of bamboo cement particle boards. In this paper, bamboo shavings are pretreated by carbonizing treatment, hydro-thermal treatment and alkali treatment; high performance liquid chromatography is adopted to analyze the influences of three different pretreatment methods on contents of water-soluble saccharides and carboxylic acid in the bamboo shavings; a Fourier infrared spectrometer and an X-ray diffractometer are respectively utilized to analyze the characteristic peak changes and crystallization property changes of chemical ingredients of the bamboo shavings before and after the three types of pretreatment. This paper discusses effects of three types of pretreatment methods in eliminating water-soluble saccharides and carboxylic acid in the bamboo shavings. Bamboo Portland cement particle boards was prepared using bamboo shavings, which are pretreated in three ways, and influences and mechanisms of different pretreatment methods on properties of the bamboo Portland cement particle boards were studied. Research indicates that the mechanical properties of the Portland cement particle board prepared from bamboo shavings pretreated with 3 % NaOH solution are superior to requirements of qualified products and superior products specified in the Standard.

Analysis of Labor Productivity in Single and Multi-household Grassland Management Patterns: A Case Study in Maqu County, Qinghai-Tibetan Plateau

This study investigated labor productivity in meat and milk/dairy production within single and multi-household management patterns, based on primary data collected from 156 randomly selected herder households in Maqu County, Tibetan Plateau. The results showed that in the rotational grazing system, herder households in both single and multi-household management patterns achieved higher labor productivity for meat production (70.36 Kg/man-day and 51.21 Kg/man-day, respectively) compared to the overall study households (40.89 Kg/man-day). In contrast, within the continuous grazing system, the single-household management pattern recorded lower labor productivity for meat production (23.04 Kg/man-day). Significantly, regional variations in the distance between pastures and market centers led herder households in the single-household management pattern within the continuous grazing system to achieve superior labor productivity for milk and dairy production (19.74 $/man-day) compared to the overall study households (15.44 $/man-day). In the rotational grazing system, labor productivity for milk and dairy production stood at 12.63 $/man-day for the single-household management pattern and 8.30 $/man-day for the multi-household management pattern. These findings underscore the complexities associated with achieving high labor productivity simultaneously in both meat and milk/dairy production within the same grassland management pattern. While the multi-household management pattern shows promise in reducing labor inputs, it also grapples with challenges in achieving substantial production levels for meat and milk/dairy products. To address these challenges, policymakers should consider follow-up measures that prioritize the simultaneous enhancement of meat and milk/dairy production within the multi-household management pattern. Special attention should be given to reducing the distance between herder households and market centers to facilitate the sale of milk/dairy products. Simply advocating for the broader adoption of the multi-household management pattern may fall short without addressing these production-related hurdles.

Biomimetic Fe7S8/Carbon electrocatalyst from [FeFe]‐Hydrogenase for improving pH‐Universal electrocatalytic hydrogen production

AbstractEfficient and cost‐effective electrocatalysts that can operate across a wide range of pH conditions are essential for green hydrogen production. Inspired by biological systems, Fe7S8 nanoparticles incorporated on polydopamine matrix electrocatalyst were synthesized by co‐precipitation and annealing process. The resulting Fe7S8/C electrocatalyst possesses a three‐dimensional structure and exhibits enhanced electrocatalytic performance for hydrogen production across various pH conditions. Notably, the Fe7S8/C electrocatalyst demonstrates exceptional activity, achieving low overpotentials of 90.6, 45.9, and 107.4 mV in acidic, neutral, and alkaline environments, respectively. Electrochemical impedance spectroscopy reveals that Fe7S8/C exhibits the lowest charge transfer resistance under neutral conditions, indicating an improved proton‐coupled electron transfer process. Continuous‐wave electron paramagnetic resonance results confirm a change in the valence state of Fe from 3+ to 1+ during the hydrogen evolution reaction (HER). These findings closely resemble the behavior of natural [FeFe]‐hydrogenase, known for its superior hydrogen production in neutral conditions. The remarkable performance of our Fe7S8/C electrocatalyst opens up new possibilities for utilizing bioinspired materials as catalysts for the HER.

Multicopy Chromosome Integration and Deletion of Negative Global Regulators Significantly Increased the Heterologous Production of Aborycin in Streptomyces coelicolor.

Aborycin is a type I lasso peptide with a stable interlocked structure, offering a favorable framework for drug development. The aborycin biosynthetic gene cluster gul from marine sponge-associated Streptomyces sp. HNS054 was cloned and integrated into the chromosome of S. coelicolor hosts with different copies. The three-copy gul-integration strain S. coelicolor M1346::3gul showed superior production compared to the one-copy or two-copy gul-integration strains, and the total titer reached approximately 10.4 mg/L, i.e., 2.1 times that of the native strain. Then, five regulatory genes, phoU (SCO4228), wblA (SCO3579), SCO1712, orrA (SCO3008) and gntR (SCO1678), which reportedly have negative effects on secondary metabolism, were further knocked out from the M1346::3gul genome by CRISPR/Cas9 technology. While the ΔSCO1712 mutant showed a significant decrease (4.6 mg/L) and the ΔphoU mutant showed no significant improvement (12.1 mg/L) in aborycin production, the ΔwblA, ΔorrA and ΔgntR mutations significantly improved the aborycin titers to approximately 23.6 mg/L, 56.3 mg/L and 48.2 mg/L, respectively, which were among the highest heterologous yields for lasso peptides in both Escherichia coli systems and Streptomyces systems. Thus, this study provides important clues for future studies on enhancing antibiotic production in Streptomyces systems.