Sunflower Oil Research Articles

PH‐responsive polyurethane‐acrylate from palm olein and sunflower oil: Sustainable synthesis, enhanced lovastatin solubility, and potential drug carrier for targeted delivery

<scp>pH</scp>‐responsive polyurethane‐acrylate from palm olein and sunflower oil: Sustainable synthesis, enhanced lovastatin solubility, and potential drug carrier for targeted delivery

Enriched oil-in-water emulsions as bioactive agents for locust bean gum films: A comparative investigation

In this study, pracaxi oil (PO) and olive oil (OO) emulsions, enriched with turmeric powder (Cur) and stabilized by nanofribrillated cellulose (NFC), were developed and incorporated to glycerol-plasticized locust bean gum (LBG) dispersions. Films were obtained by solution casting, and their properties were compared, aiming to expand PO application as a potential constituent of active food packaging. The films were characterized for their morphological, structural, transparency, gas permeability, wettability, thermal, mechanical, and antioxidant properties. In relation with the plasticized LBG film, both emulsion films showed improved humidity content, surface wettability, oxygen barrier capacity, thermal stability, and mechanical properties. As for wettability, while the initial water contact angle for the LBG film was θ0 = 93.4°, values of θ0 = 105.5° and θ0 = 99.2° were determined for the LBG films incorporated with PO- and OO-based emulsions, respectively. In comparison with the LBG film, the incorporation of PO-based droplets led to an approximately 8.0% reduction in the water vapor transmission rate values. Also, in comparison with the LBG film, the LBG/NFC/PO-Cur and LBG/NFC/OO-Cur films were capable of reducing the peroxidation value of sunflower oil by 16% and 11%, respectively. Enhancements in tensile strength values of 44% and 49% were determined for the PO- and OO-based emulsion films. The emulsion films presented similar antioxidant properties and better preservation of strawberry model food than LBG and polyethylene films. The results of the comparative experiments suggested that the use of PO may be expanded as a component of active food packaging.

Tissue culture and Agrobacterium-mediated genetic transformation of the oil crop sunflower

Sunflower is one of the four major oil crops in the world. ‘Zaoaidatou’ (ZADT), the main variety of oil sunflower in the northwest of China, has a short growth cycle, high yield, and high resistance to abiotic stress. However, the ability to tolerate adervesity is limited. Therefore, in this study, we used the retention line of backbone parent ZADT as material to establish its tissue culture and genetic transformation system for new variety cultivating to enhance resistance and yields by molecular breeding. The combination of 0.05 mg/L IAA and 2 mg/L KT in MS was more suitable for direct induction of adventitious buds with cotyledon nodes and the addition of 0.9 mg/L IBA to MS was for adventitious rooting. On this basis, an efficient Agrobacterium tumefaciens-mediated genetic transformation system for ZADT was developed by the screening of kanamycin and optimization of transformation conditions. The rate of positive seedlings reached 8.0%, as determined by polymerase chain reaction (PCR), under the condition of 45 mg/L kanamycin, bacterial density of OD600 0.8, infection time of 30 min, and co-cultivation of three days. These efficient regeneration and genetic transformation platforms are very useful for accelerating the molecular breeding process on sunflower.

Mesoporous silica aerogels for sunflower oil refining and investigation of their adsorption performance

AbstractInvolving a succession of oil refining stages for edible oil production, a notable constraint lies in the necessity to employ diverse adsorbents at various steps within these processes. This study investigates the synthesis of mesoporous silica aerogels from rice husk ash, comparing their efficacy in physical sunflower oil refining with earth clay (Bentonite) and commercial silica (Trisyl). Tetraethyl orthosilicate (TEOS) impact during aging was analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and N2 adsorption-desorption analyzer to examine alterations in the structure of silica aerogels. The surface areas of TEOS-doped silica aerogel (TSA) and non-TEOS-doped silica aerogel (NTSA) were 296.18 and 267.06 m²/g. Mesoporous silica aerogels were evaluated for their ability to reduce free fatty acids (FFA), peroxide value (PV), phosphorus, and color pigments in sunflower oil. TSA and NTSA demonstrated significant FFA removal, with TSA at 3 wt.% achieving the highest performance of 32.2%. TSA also effectively reduced PV and phosphorus compared to NTSA, Bentonite and Trisyl, exhibiting performance similar to Bentonite in the bleaching process. TEOS-doped silica aerogels have shown promise as adsorbents for impurity removal in sunflower oil and has emerged as the potential adsorbent that can comprehensively and effectively meet the requirements of many edible oil physical refining applications in a singular step. Graphical Abstract

Improvement of the oxidative stability of edible oils through enzymatic esterification with hydroxytyrosol‐rich extract

AbstractOxidative degradation is a common issue of commercial edible oils that results in rancidity, downgrading the organoleptic characteristics of food matrices. The aim of this study was the enzymatic modification of common vegetable oils (pomace olive and sunflower oil) with polyphenolic extract to prepare functional oils enriched with bioactive phenolipids. Under that scope, an agro‐industrial effluent, olive mill wastewater, was used as source to recover phenolic compounds. The recovery of hydroxytyrosol and tyrosol reached up to 81.2% ± 0.3% and 98.3% ± 0.2%, respectively. Further, immobilized lipase was used to catalyze the one‐step acylation of these phenolic antioxidants into the oils with the aim of enhancing their stability under accelerated oxidation conditions. The optimum parameters of the bioconversion were found to be 0.1% (w/w) immobilized lipase, 24h of incubation at 50°C under orbital shaking reaching high conversion yields for both phenolic compounds (≥94%). The results also highlighted that the extract‐modified oils presented notable thermooxidative stability at 60°C for 28 days compared to the control and synthetic antioxidant‐enriched oils, as demonstrated by several analytical (conjugated dienes, thiobarbituric reactive substances), chromatographic (solid‐phase microextraction gas chromatography/mass spectroscopy), and spectroscopic (fluorescence, attenuated total reflectance) techniques, giving new insights regarding the preservation of edible oils.Practical Application: Htyr has recently gained much attention as an active ingredient of food matrices for industrial applications due to its high antioxidant/antiradical activity. A major drawback of Htyr exploitation is the high market price and its solubility. Meanwhile, several edible oils are prone to autooxidation during transport, stock management, and household storage. The proposed one‐step biocatalytic process aims to enhance the lipophilicity and bioactivity of Htyr in bulk oil systems, making the use of this potent antioxidant compound feasible. This approach shows promise in extending the shelf life of common oils and highlights the advantages of by‐product management. Moreover, these functional oils can be applied for the preparation of sauces, bakery products, food supplements, and so on, as the bioactive ingredients that are present in them can delay the oxidative cascade reactions and, thus, the spoilage of the products, whereas the whole process meets the requirements of circular economy and green chemistry.

Production of Camellia oleifera Abel Seed Oil for Injection: Extraction, Analysis, Deacidification, Decolorization, and Deodorization

Camellia seed oil (CSO), as a nutrient-rich edible oil, is widely used in foods, cosmetics, and other fields. In this work, the extraction, deacidification, decolorization, and deodorization processes of CSO were respectively optimized for meeting injectable oil standards. The results showed that the CSO extraction rate reached the highest level of 94% at optimized conditions (ultrasonic time, 31.2 min; reaction pH, 9.2; and reaction time, 3.5 h). The physicochemical indexes of CSO and 10 other vegetable oils were evaluated by the principal component analysis method, and the overall scores of vegetable oils were ranked as camellia seed oil &gt; olive oil &gt; rice oil &gt; peanut oil &gt; sesame oil &gt; corn oil &gt; soybean oil &gt; sunflower oil &gt; rapeseed oil &gt; walnut oil &gt; flaxseed oil. The physicochemical indicators of CSO were the most ideal among the 11 vegetable oils, which means that CSO is suitable as an injectable oil. Through the optimized processes of the deacidification, decolorization, and deodorization, the CSO acid value was reduced to 0.0515 mg KOH/g, the decolorization rate reached a maximum of 93.86%, and the OD430 was 0.015, meeting the requirement (≤0.045 of OD430) of injectable oil. After the deodorization process, these parameters of the refractive index, acid value, saponification value, iodine value, absorbance, unsaponifiable, moisture and volatiles, fatty acid composition, and heavy metal limits all met the pharmacopoeia standards of injectable oil in many countries and regions. The possibility of CSO as an injectable oil was first verified through refining-process optimization and nutritional index analysis, providing an important technical reference for the high-value utilization of vegetable oil.

Training on Processing Sunflower Seed Oil (Helianthus annus L.) into Massage Oil

Massage treatment is part of traditional health services which can provide a stimulating and relaxing effect, improve the blood and lymph circulation system and strengthen other body systems, intended to improve health and fitness. Massage treatments generally use massage oil containing essential oils as aromatherapy. The use of essential oils in aromatherapy, especially through massage treatments, must be mixed with carrier oil in the form of vegetable oil such as sunflower seed oil. Pengalangan village is a sunflower agrotourism village with great sunflower potential but the results of sunflower cultivation are still less than optimal and are limited to sunflower seed food. Therefore, the team provides an Eco-preneurship solution with an effort to increase the selling value of the results of processing sunflower seeds in the form of oil to be processed into massage oil. This effort was carried out by providing material using the lecture method and continued with training in making massage oil for PKK cadres and village residents as participants. The end of this activity showed an increase in participants' knowledge by 16.8%, as well as an increase in participants' skills in making massage oil products independently.

Enhancing high-speed CNC milling performance of Ti6Al4V alloy through the application of ZnO-Ag hybrid nanofluids

Abstract This research investigates the performance of high-speed CNC milling operations on Ti6Al4V alloy by employing a novel ZnO-Ag hybrid nanofluid. The study involves the preparation and characterization of nanofluids with varying concentrations of nanoparticles, focusing on thermal conductivity and stability. The machining experiments encompass four critical input parameters: Minimum Quantity Lubrication (MQL) flow rate, cutting speed, nanofluid concentration, and feed rate. Performance evaluation is based on average surface roughness (Ra) and cutting temperature. Key findings include a remarkable 21.05% improvement in thermal conductivity for the ZnO-Ag-based sunflower oil at 0.2% volume concentration compared to 0.05% concentration. The prepared nanofluids exhibit good stability. Moreover, cutting speed and MQL flow rate emerge as significant contributors to Ra, accounting for 35.62% and 34.82%, respectively. Interestingly, MQL flow rate is identified as the most influential factor, surpassing even cutting speed. SEM images for tool wear reveals that the ZnO-Ag based sunflower oil reduced tool wear significantly. In conclusion, the proposed ZnO-Ag-based sunflower oil at 0.2% concentration emerges as the good option for sustainable high-speed machining of Ti6Al4V alloy, showcasing&amp;#xD;

Absolute kinetics of peroxidation and antioxidant protection of intact triglyceride vegetable oils

To verfy their difference from isolated fatty acids, the absolute kinetics of peroxidation was studied for seven triglyceride-based oils of olive (OLI-1, OLI-2), high-oleic sunflower (SUN-HO), high-oleic and high-linoleic safflower (SAF-HO, SAF-HL) grapeseed (GRA) and borage (BOR), by oxygen uptake monitoring, using 2,6-di-tert-butyl-4-methoxyphenol and 2,2,5,7,8-pentamethyl-6-chromanol as reference inhibitors. Propagation constants (kp/M−1 s−1 at 303 K in PhCl) were respectively 34.8 ± 2.3, 35.1 ± 1.8, 40.6 ± 5.5, 36.0 ± 7.7, 160.8 ± 5.1, 145.1 ± 24.5, 275.1 ± 63.8, while oxidizability responded to empirical equation kp(2kt)-½/M-½s-½ = 1.63 × 10−3[allyl >CH2/M] + 1.82 × 10−2[bisallyl >CH2/M], based on fatty acids residues assessed by GC–MS. Peroxidation kinetics was markedly different from that of isolated fatty acids. The H-bond basicity of all oils was measured by FT-IR affording Abraham's βH2 values in the range 0.55 ± 0.03. H-bonding explained the protection of oils measured for seven reference phenolic antioxidants, except for the catechols quercetin and caffeic acid phenethyl ester, which were 2-to-4-folds more effective than expected, supporting a proposed different mechanism.

Nutritional and Organoleptic Characteristics of Sausage Based on an Autochthonous Ecuatorian Fish, Old Blue (Andinoacara rivulatus).

The consumption of fisheries and aquaculture products has been increasing in recent decades, and it is necessary to strike a balance between production and sustainability in aquaculture; this is essential homework to support the demand for human food. This study aimed to investigate the sensory and nutritional characteristics of a sausage made from the fillet of the native fish Old Blue (Andinoacara rivulatus) to stimulate the local economy. A multifactorial A*B*C design was used, with Factor A being rearing systems (wild and farmed), Factor B being types of protein (quinoa meal and soybean meal), and Factor C being lipids of animal (pork fat) and vegetable (sunflower oil) origin. Highly significant differences were observed in all formulas, according to Tukey (p < 0.05). The highest protein percentage was with soybean flour at 11.24%, while quinoa flour had 10.80% of the product. In sensory characteristics, the best attributes were for texture in the mouth with a hedonic scale from 0 to 5, with 4.2 firmness being mostly acceptable, odor at 4.5, the aromatic attribute with the highest and best value, and color was the clearest at 4.3 acceptability. The flavor was 4.3, mostly more pleasant according to the tasters. The yield of farmed fillets was 23.16% compared to wild 13.89%, and the balance of sausage yield was 393 g with a yield of 76.33% of the total weight. Microbiological analysis of the sausage with the native species showed the absence of pathogenic microorganisms. A commercial shelf life of 30 days was also established for the sausage, demonstrating an added value through this processing, allowing its exploitation in areas further away from the fishing sites and, thus, a greater possibility of social development in the area.

Thermoreversible oleogelation of fatty acid monoglyceride-containing sunflower oil from a rheological approach

Nowadays, there is a growing concern among consumers about the impact of diet on health. In this regard, oleogelation has been proposed as a promising strategy to replace unhealthy fats, as well as to encapsulate water-insoluble bioactive compounds in food matrices. Consequently, the development of thermoreversible oleogels may play an important role in the bioavailability and biostability of functional foods and the beneficial effects associated with them. The aim of this work was to evaluate the viscoelastic behavior of oleogels using a commercial food additive (E471) composed of fatty acid monoglycerides (MG). Furthermore, the study of sol-gel transitions of these thermoreversible oleogels allowed to obtain the optimum concentration of gelling agent required. Refined sunflower oil containing 2-5 wt.% of E471 were characterized by means of SAOS tests, as a function of temperature. All these systems were liquids above 48°C, forming stable oleogels below 20°C. An improvement of the elastic component of them was observed as gelling agent concentration was higher. Oleogels containing 4 and 5 wt.% MG showed very similar viscoelastic properties and high stability at body temperature (ca. 37°C). Conversely, systems containing 2% or 3% led to the formation of weaker gels exhibiting thermo-reversibility but only the latter was stable at body temperature. Therefore, sunflower oleogels containing MG above 3 wt.% could be regarded as potential carrier food matrices for lipid-soluble active ingredients. To this end, the modulation of the oleogel network consistency should play a key role in maintaining the integrity of the active principle during gastrointestinal transit.

Multifunctional Hydrophobin-Like Protein (HFB-NJ1): A Versatile Solution for Wastewater Treatment

As wastewater contains a variety of contaminating bacteria and oily residues, there is an urgent need for environmentally safe bactericidal agents and surfactants which can be applied for wastewater treatment. The present study emphasizes on the potential of hydrophobin-like protein (HFB-NJ1) extracted from sporulating mycelia of Aspergillus sp. NJ1 for wastewater treatment. The purified HFB-NJ1, depicted the presence of one single protein band of molecular size approximately 11-12 kDa on silver-stained SDS-PAGE gel. HFB-NJ1 also presented properties such as surface modification of glass and stable emulsification of sunflower oil. HFB-NJ1 depicted exceptional antibacterial activity against bacterial pathogens such as Bacillus subtilis and Pseudomonas aeruginosa at low MIC of 0.5 μg/mL and 0.75 μg/mL respectively. Additionally, HFB-NJ1 depicted enhanced emulsification of various vegetable and petroleum-based oils (E24 > 80%). HFB-NJ1 effectively reduced gold ions, producing nanospheres with a size of 15.33 nm – a recognized antimicrobial agent. This underscores the multifunctional attributes of HFB-NJ1, highlighting its efficacy in removing pathogenic bacteria, emulsifying organic compounds from wastewater, and demonstrating a reduction ability for nanoparticle synthesis.

Evaluation of antimicrobial activity in contaminated root canals and cytotoxicity of ozonized products: an ex-vivo study

Pulp and periapical diseases originate from microbial colonization of root canals. The development of new technologies and instrumentation techniques has provided more effective shaping of the root canal system. Even so, sodium hypochlorite and calcium hydroxide remain examples of auxiliary substances for endodontic treatment. Recently, ozone has emerged as promising in endodontic therapy. This study evaluated the antimicrobial activity of ozonized water and ozonized oils in root canals infected with Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in addition to the cytotoxicity of ozonized products. 224 human lower premolars were used, divided into 4 groups according to the microorganism tested, challenged with ozonized medications and compared to sodium hypochlorite and calcium hydroxide. The antimicrobial activities of ozonized oils were comparable to those established by sodium hypochlorite and calcium hydroxide. Ozonated sunflower oil and olive oil exhibited lower cytotoxicity and showed promise for understanding new metabolic routes and additional approaches in Endodontics.

Antioxidant and antimicrobial extracts from grape stalks obtained with subcritical water. Potential use in active food packaging development

In order to valorise winemaking grape stalks, subcritical water extraction at 160 and 180 °C has been carried out to obtain phenolic-rich extracts useful for developing active food packaging materials. Red (R) and white (W) varieties (from Requena, Spain) were used, and thus, four kinds of extracts were obtained. These were characterised as to their composition, thermal stability and antioxidant and antibacterial activity. The extracts were incorporated at 6 wt% into polylactic acid (PLA) films and their effect on the optical and barrier properties of the films and their protective effect against sunflower oil oxidation was analysed. Carbohydrates were the major compounds (25–38%) in the extracts that contained 3.5–6.6% of phenolic compounds, the R extracts being the richest, with higher radical scavenging capacity. Every extract exhibited antibacterial effect against Escherichia coli and Listeria innocua, while PLA films with extracts preserved sunflower oil against oxidation.

Improving environmental indicators of the wheeled tractor diesel engine by using biofuels

The object of this study is the process of operation of a diesel engine in a wheeled tractor on methyl ether of spent frying sunflower oil. The task being solved is to improve the environmental performance of a diesel engine in a wheeled tractor during the operation of its diesel on such biodiesel fuel and its mixtures with mineral diesel fuel. The characteristics of the diesel D-243 were determined by the experimental method with the measurement of the toxicity of the waste gases during its operation on the methyl ether of the spent sunflower oil, mineral fuel, and their mixtures. A decrease in the content of soot in diesel exhaust gases and a slight increase in the types of nitrogen oxides was registered. The theoretical method using a mathematical model determined the indicators of a wheeled tractor during its movement with a trailer for the accepted driving cycle and the operation of its diesel on different fuels. To that end, the characteristics of a specific diesel engine were described by polynomial models. The adequacy of the mathematical model of the movement of a tractor with a trailer over a driving cycle was tested. By means of a mathematical model, the total road emissions of harmful substances of a diesel engine were calculated when the tractor is running with a trailer over the accepted driving cycle. Calculations were performed for two types of fuel: mineral diesel fuel and biodiesel fuel. Biofuel consumption increases by almost 10 % compared to diesel fuel. The total emissions of harmful substances are 1.1 times lower in a diesel engine running on biofuel than when using mineral fuel. The results could be used in the operation of technological transport in the industry and agriculture provided there is a sufficient volume of raw materials for the production of biofuel

Development of conditions for obtaining oil from sunflower oil hydration waste

Development of conditions for obtaining oil from sunflower oil hydration waste

Development of an emulsion composition with fennel and caraway essential oils for use in the combined therapy of ulcerative colitis

The aim of the study is to develop the composition of an emulsion containing essential oils of Fennel and Caraway seeds for use in the symptomatic complex therapy of ulcerative colitis with the aim of eliminating functional intestinal disorders. Materials and methods. The objects of the study were samples of emulsions containing active pharmaceutical ingredients (essential oils of Fennel and Caraway), purified water, oils (refined sunflower oil, refined olive oil, refined sesame oil), emulsifiers (polyethylene glycol 40 hydrogenated castor oil, polysorbate 80, polyethylene glycol 100 stearate, acacia gum, guar gum, xanthan gum, soya lecithin), viscosity regulator - apple pectin and flavouring agents (food additives with cherry and tarragon flavour). Organoleptic properties, stability, rheological parameters, pH, particle size determination by microscopy, and a taste test were carried out with model emulsion samples. Research to establish the optimal technological parameters was carried out in parallel. Results. The main parameters of the technological process have been established, which allow to obtain an emulsion with evenly distributed particles: 15 minutes at maximum speed. The concentration of emulsifiers at which the emulsions are stable was selected. It was found that the samples containing polyethylene glycol 100 stearate, gums, and soy lecithin have satisfactory organoleptic properties. The sample with soy lecithin emulsifier differs from the others in its ability to form microemulsions, but it has low viscosity. To improve the rheological properties, apple pectin was added to the emulsion. The taste test showed that among vegetable oils, refined sesame oil has a more neutral taste, and the flavouring additive "Tarkhun" balances the taste better. The release of active pharmaceutical ingredients (APIs) from the emulsion base was confirmed by thin-layer chromatography. Conclusions. A microemulsion with essential oils based on refined sesame oil, soy lecithin, with the addition of viscosity and flavour correctors was developed. The obtained emulsion has satisfactory organoleptic properties and conforms to the requirements for emulsion quality indicators

The Impact of Utilizing Waste Sunflower Oil as a Biodiesel Blend on Four-Stroke Engine Performance and Emissions

The blending of biodiesel with petroleum diesel attracts much attention due to its high potential in reducing emissions. In this work, waste sunflower oil was converted to biodiesel by the trans-esterification method, and it was blended with petroleum diesel in three ratios (10, 30, and 50%). The impact of using these blended fuels in a four-stroke engine on engine performance and exhaust emissions at three engine loads (2, 4, and 6 N.m) was investigated and compared with the use of petroleum diesel and biodiesel. The engine performance was evaluated by determining the brake-specific fuel consumption (BSFC), engine effective power (Ne), brake-specific energy consumption (BSEC), brake thermal efficiency (BTE), and noise intensity. The evaluation of emissions from the engine exhaust was carried out by measuring the levels of carbon oxides (CO and CO2), hydrocarbons (HC), nitrogen oxides (NO and NO2), and particulate matter (PM). The results show that blending diesel with up to 30% biodiesel can reduce CO, HC, and PM emissions by 29.6 ± 1%, 26.0 ± 4%, and 31.0 ± 3%, respectively. However, this decrease is associated with increasing CO2 and NOx emissions by 18.5 ± 2.5% and 29.0 ± 6%, respectively. In addition, the engine showed acceptable performance when using up to 30% biodiesel, where the increase in fuel consumption was limited to 5.8 ± 0.3%. In addition, the engine’s effective power increased with the blending ratio of 10% by 2.0 ± 0.6%, but then decreased with the blending ratio of 30% by only 2.0 ± 0.6%. The noise intensity was also decreased by 2.4%, while BSEC and BTE were reduced by only 2.9 ± 0.9% and 3.5 ± 1%, respectively. The results of this work provide deep insights regarding the utilization of waste sunflower oil as biodiesel to be blended with petroleum diesel, which is a considerable novel approach in the energy and environmental sectors.

Oral Supplementation of Ozonated Sunflower Oil Augments Plasma Antioxidant and Anti-inflammatory Abilities with Enhancement of High-Density Lipoproteins Functionality in Rats

Research on ozonated sunflower oil (OSO) is mostly restricted to its topical application, whereas the functional and toxicological assessment of oral OSO consumption is yet to be solved. Herein, OSO was orally supplemented in rats to assess the impact on plasma antioxidant status, low-density lipoproteins (LDL), and high-density lipoproteins (HDL). Also, the functionality of HDL from the OSO-supplemented rats (OSO-HDL) was tested against carboxymethyl-lysine (CML)- induced hyperinflammation in embryo and adult zebrafish. The results revealed that four weeks of OSO supplementation (3 g/kg BW/day) had no adverse effect on rats’ hematological and blood biochemical profiles. Nonetheless, decreased interleukin (IL)-6, and LDL-C levels, along with enhanced ferric ion reduction ability (FRA) and sulfhydryl content, were observed in the plasma of OSO-supplemented rats compared to the control and sunflower oil (SO) supplemented group. In addition, OSO supplementation stabilized apoA-I/HDL and augmented HDL-allied paraoxonase (PON)-1 activity. The microinjection of OSO-HDL (10 nL, 2 mg/mL) efficiently prevented the CML (500 ng)-induced zebrafish embryo mortality and developmental deformities. Similarly, OSO-HDL thwarted CML-posed neurotoxicity and demonstrated a significant hepatoprotective effect against CML-induced fatty liver changes, hepatic inflammation, oxidative stress, and apoptosis, as well as exhibiting a noticeable influence to revert CML-induced dyslipidemia. Conclusively, OSO supplementation demonstrated no toxic effects on rats, ameliorated plasma antioxidant status, and positively influenced HDL stability and functionality, leading to a protective effect against CML-induced toxicity in zebrafish.

The use of ozonized sunflower oil in the treatment of wounds in small animals

A pele, como camada protetora do corpo, é altamente suscetível a lesões, tornando a cicatrização um tema de grande relevância na medicina veterinária, especialmente na clínica de pequenos animais, devido à alta casuística. O presente trabalho visa avaliar o efeito cicatrizante do óleo de girassol ozonizado no tratamento de feridas em animais, através de dois casos clínicos em espécies distintas. Foram estudadas duas feridas cutâneas com miíase, uma em um canino sem raça definida, com lesão na região perianal e outra em um felino sem raça definida, com lesão na região cervical, ambas as feridas foram tratadas com óleo de girassol ozonizado. A terapia não apresentou efeitos colaterais em nenhum dos animais. Desse modo, o tratamento foi eficaz, no qual a cicatrização completa das feridas ocorreu em 30 dias para o canino e 50 dias para o felino. Além da cicatrização, os animais apresentaram crescimento acelerado dos pelos, possivelmente relacionado ao efeito do óleo de girassol ozonizado na circulação e no metabolismo. A terapia instituída pelo ozônio influenciou positivamente nas feridas, onde a mesma agiu no local, promovendo oxigenação e melhorando o metabolismo dos animais. O óleo de girassol ozonizado contribuiu para a redução da infecção local, atuando como bactericida, analgésico e anti-inflamatório. A ozonoterapia é uma terapia integrativa e age de forma coadjuvante aos tratamentos convencionais. Essa terapia vem sendo cada vez mais utilizada na medicina veterinária nas suas diversas possibilidades de aplicação. O tratamento tópico de óleo de girassol ozonizado demonstrou ser totalmente eficaz na regeneração de ambas as feridas, ocorrendo em um período mais curto de tempo no cão do que no gato devido às particularidades de cada espécie, além de ser um tratamento de fácil aplicação e de custo baixo aos tutores.