Abstract
Pulp and periapical diseases originate from microbial colonization of root canals. The development of new technologies and instrumentation techniques has provided more effective shaping of the root canal system. Even so, sodium hypochlorite and calcium hydroxide remain examples of auxiliary substances for endodontic treatment. Recently, ozone has emerged as promising in endodontic therapy. This study evaluated the antimicrobial activity of ozonized water and ozonized oils in root canals infected with Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in addition to the cytotoxicity of ozonized products. 224 human lower premolars were used, divided into 4 groups according to the microorganism tested, challenged with ozonized medications and compared to sodium hypochlorite and calcium hydroxide. The antimicrobial activities of ozonized oils were comparable to those established by sodium hypochlorite and calcium hydroxide. Ozonated sunflower oil and olive oil exhibited lower cytotoxicity and showed promise for understanding new metabolic routes and additional approaches in Endodontics.
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