Summer-autumn Period Research Articles

Dataset of benthic copepods in the littoral zones of Lake Maggiore

Copepods (Crustacea: Copepoda) are widespread in aquatic ecosystems worldwide and represent an important component of the meiobenthic metazoan assemblages of lake littorals. Yet, little is currently known about the diversity of benthic copepods in lentic habitats. We gathered information on the benthic copepod assemblages of the littoral zone of Lake Maggiore as part of the Interreg Italy-Switzerland 2014-2020 Parchi Verbano Ticino project (ID: 481668), funded by the European Regional Development Fund (ERDF). Lake Maggiore is a large and deep temperate lake in north-western Italy human-managed from mid-March to mid-September. This contributes to substantial changes in water levels in relation to human activities such as hydropower, industrial and agricultural uses. We compiled a dataset listing 234 occurrences of benthic copepod species recorded from three littoral sampling sites situated in three protected areas, two of which are part of the Natura 2000 network in Italy and one is part of the Emerald Network in Switzerland. We collected the specimens during the summer-autumn period in 2019-2021. We identified the copepods at the species level and created a dataset with individual georeferenced occurrence records for each species, organized in a standardized Darwin Core Archive format. We made available, through the Global Biodiversity Information Facility (GBIF), a total of 13 distinct taxonomic entities and 234 unique georeferenced occurrence records related to the benthic copepod assemblages of the littoral zone of Lake Maggiore (Tabilio Di Camillo et al., 2023). The dataset has the potential to support the authorities in charge of managing Lake Maggiore’s water levels in addressing the ecological risk relevant to the littoral zone and in developing shared implementation strategies for sustainable water management.

Formation of highly productive agrophytocenoses of <i>Raphanus sativus</i> L. subsp. <i>acanthiformis</i> (Blanch.) Stankev. in the conditions of the North Caucasus

Relevance. One of the most important tasks of agricultural production is to provide the population of the regions with vegetables at the expense of local production. Despite the social significance of the vegetable growing industry, its development in the Central Ciscaucasia at the present stage allows only half to meet the demand of the population. For the Chechen Republic, where the area of arable land is limited, the daikon (Raphanus sativus L. subsp. acanthiformis (Blanch.) Stankev.) Has a certain perspective, especially for cultivation in the summer-autumn period, after early harvesting of vegetables and field crops.Results. Under the conditions of the Chechen Republic, the maximum yield of root crops (29.0 t/ha) and marketability of 65% of the early maturing variety Sasha was obtained with a sowing time of 31.07 and a scheme of 35x10 cm. The highest yield of root crops of varieties Dubinushka, Dragon, Favorit was formed at a sowing time of 17.07 (58.1, 62.3 and 57.3 t/ha, respectively), and variety Moskovsky Bogatyr – at a sowing time of 3.07 (72.3 t/ha) at sowing scheme 70x10 cm.

Mass spectrometry-based metabolite profiling reveals functional seasonal shifts in the metabolome of Zygophyllum dumosum Boiss and its relation to environmental conditions

Main conclusionA multi-year study of perennial Z. dumosum shows a consistent seasonal pattern in the changes of petiole metabolism, involving mainly organic acids, polyols, phenylpropanoids, sulfate conjugates, and piperazines.GC–MS and UPLC–QTOF-MS-based metabolite profiling was performed on the petioles of the perennial desert shrub Zygophyllum dumosum Boiss (Zygophyllaceae). The petioles, which are physiologically functional throughout the year and, thus, exposed to seasonal rhythms, were collected every month for 3 years from their natural ecosystem on a southeast-facing slope. Results showed a clear multi-year pattern following seasonal successions, despite different climate conditions, i.e., rainy and drought years, throughout the research period. The metabolic pattern of change encompassed an increase in the central metabolites, including most polyols, e.g., stress-related D-pinitol, organic and sugar acids, and in the dominant specialized metabolites, which were tentatively identified as sulfate, flavonoid, and piperazine conjugates during the summer–autumn period, while significantly high levels of free amino acids were detected during the winter–spring period. In parallel, the levels of most sugars (including glucose and fructose) increased in the petioles at the flowering stage at the beginning of the spring, while most of the di- and tri-saccharides accumulated at the beginning of seed development (May–June). Analysis of the conserved seasonal metabolite pattern of change shows that metabolic events are mostly related to the stage of plant development and its interaction with the environment and less to environmental conditions per se.

Clinical characteristics of intestinal mixed infections

Objective. To study the features of the clinical picture of mixed intestinal infections in patients treated at the Regional Infectious Clinical Hospital named after A.M. Nichoga in the period from 2017 to 2021.
 Materials and methods. The clinical picture was studied in 55 patients with mixed intestinal infection who were treated at the Regional Infectious Hospital named after A.M. Nichoga (Astrakhan).
 Results. Mixed intestinal infections in most cases were registered in the summer-autumn period (51 % 92.7 %) in the form of sporadic morbidity. Epidemiological anamnesis data show that the majority of patients drank unboiled water, swam in the river, where they accidentally swallowed water, consumed food products of questionable quality, chicken meat, eggs insufficiently heat-treated, unwashed vegetables and fruit. Four patients arrived in Astrakhan from amoebiasis-endemic regions (Uzbekistan, Turkmenistan, Turkey, Egypt); 51 patients (92.7 %) had a moderate form of the disease,4 patients (7.3 %) a severe form. The clinical picture of mixed intestinal inflections was characterized by the symptoms of shigellosis and salmonellosis.
 Two patients, diagnosed shigellosis + amoebiasis had manifestations of ulcerative colitis complicated by intestinal bleeding; 2 patients, diagnosed salmonellosis + amoebiasis, developed dehydration shock. The treatment of patients with a severe form was carried out in the Intensive Care Unit and Resuscitation Unit of the Regional Infectious Clinical Hospital.
 Conclusions. The clinical picture of mixed bacterial intestinal infections and intestinal amoebiasis is mainly determined by the symptoms of shigellosis and salmonellosis and is manifested by the phenomena of intoxication, dehydration and the development of colitic and gastroenterocolitic syndromes.

Ecological and hydrochemical analysis of the rivers Drevlianskyi nature reserve (Ukraine)

Problem statement. The quality of the surface water and ecological condition plays a big role in ecosystems, especially in the hydrochemical regime. The chemical composition and mineralization of river water are in a dynamic link and are formed under the influence of many external factors. Therefore, under the influence of the latter, there was a need to study the hydrochemical composition of the water reserve, which was an urgent issue of the work. The purpose of the research: comprehensive ecological, hydro-chemical analysis of the state of the rivers of the «Drevlyanskyi» nature reserve for the optimization of environmental protection measures, monitoring, eco-safety and preservation of the biodiversity of hydrobionts. Research methodology. Conducting research on water quality was based on classical, generally accepted methods: field research, literature review, laboratory research, analysis of the obtained results. Results. According to research, it was established that within the nature reserve in the summer-autumn period, the water of the main Uzh River and its tributary is moderately fresh in terms of mineralization (0.1–0.6 g/dm3), neutral in pH, and has a hydrocarbon-calcium chemical composition. Insignificant waterlogging of landscapes determines the low content of ammonium nitrogen and dissolved iron in river water. The Zvizdal and Osliv rivers are exception where eutrophication is observed which cauces high runoff of organic substances. It was also investigated that During the summer-autumn period of low water the water contained small amounts of microelements and heavy metals (with the exception of Fe, Mn) which don’t exceed the established sanitary-hygienic maximum allowable concentrations. According to the classification proposed by O.O. Alekin all-natural waters are divided into three classes. According to the content of the main ions and the mineralization of the water, it was analyzed that the water bodies of the nature reserve belong to the calcium hydrogen-carbonate, class of type III. In the water bodies of the nature reserve Drevlianskyi there are water subtypes IIIa-IIIb. The last differ from each other by the presence of magnesium hydrogen carbonate Mg(HCO3)2 in IIIa and calcium sulfate CaSO4 in IIIb. Scientific novelty. For the first time, a comprehensive analysis of surface waters in radiation-contaminated territories was carried out under the conditions of complete bequest. Practical significance and research perspectives. The practical value of the research materials lies in the further monitoring of hydroecosystems, the substantiation of environmental protection measures, and the results will be used in the annual "Chronicle of the Nature" program of the reserve for the Ministry of Environmental Protection and Natural Resources of Ukraine.

Филлофаги древесных растений в рекреационно-озеленительных насаждениях Волгограда

The article provides general information on the taxonomic composition and features of the quantitative abundance of arthropods-phyllophages of woody plants that form the basis of recreational and landscaping plantings of the urbanized territory of Volgograd. Entomological studies were carried out in 2020–2022 in landscaping plantings of different types and categories by visual inspection of foliage and needles, manual collection of insects and collection by mowing with an entomological net in the spring-summer and summerautumn periods. As a result of long-term entomological studies, 108 species of phyllophages were identified on the examined tree species. For the most widespread pest species, data on population density, extensiveness and intensity of damage to tree species (elm, poplar, robinia, pine) are presented. The analysis of the taxonomic abundance of phyllophages revealed in the composition of the considered complex, including alien species of insects for the fauna of the region, damaging woody plants in recreational and landscaping plantings.

Связи изменений ледовой обстановки на Северном морском пути с движением планет Солнечной системы

The paper considers the progress in the hydrographic support for navigation in various parts of the Northern Sea Route in terms of improving the methods for developing tentative forecasts of interannual changes of ice conditions. Among the approaches to solve the task the authors take into account factors that do not depend on the uncertainty of regional climate changes in the future. One of such factors is the main mechanical process in the Solar System — the orbital planetary motion. The paper goal is to confirm the existence of water areas in the seas under study, changes in the ice coverage of which in the summer-autumn period can be significantly associated with planetary orbital motion factor. The authors apply statistical methods to achieve the study goal. As factual material on changes in the ice coverage of the sections of the Northern Sea Route, they use information of GLORYS12v1 and ICDC global reanalysis databases for the period 1993—2019 and 1979—2020 respectively. As a result the authors have revealed locations of the water areas, for which the reliability of the conclusion about the significance of the relationship under consideration in certain months is at least 90%. They have stated that the reliability of such inference is the highest for some water areas of the East Siberian Sea in October and the Chukchi Sea in November. To confirm the suitability of the identified links for the development of tentative forecasts of trends in interannual changes in the ice conditions on the Northern Sea Route, the authors insist on additional testing to check the resistance to updating the actual material.

Correlation analysis of blood biochemical and morphological markers in small breeds dogs with obesity

Purpose : the study of biochemical and morphological indicators of the blood of dogs of small breeds with obesity depending on age, breed and weight. Materials and methods . The analysis of biochemical and morphological indicators of blood 21 dogs of small breeds in the summer-autumn period was analyzed. The age distribution was from 3 to 13 years. In the blood serum, the level of total protein, albumin, globulin, urea, creatinine, total calcium, phosphorus, the activity of aspartateataminostrostrostrase (asat), alanineine -orgrase (alat), alkaline phosphatase were determined. In stabilized blood, indicators of hematocrit, hemoglobin, the number of red blood cells, leukocytes and platelets were determined. The determination of blood indicators was carried out according to generally accepted methods. Results . Higher indicators of the activity of asat enzymes and alkaline phosphatase in the absence of a significant increase in the activity of Alat, the activity of which will increase primarily with the liver damage, were identified. When evaluating red blood indicators, the most visual change was a decrease in the level of hemoglobin and hematocrit, both with the course of age and with an increase in body weight. This may indicate inhibition of hematopoietic function in animals with increased fat mass of the body, possibly due to chronic prolonged inflammation as a result of the release of inflammatory adipokins with fat tissue. The chronic damaging effect of the presumably adipokins of adipose tissue, expressed in the non -specific increase in the activity of blood serum enzymes, anemia of chronic inflammation and moderate leukocytosis in the absence of obvious clinical signs. All of these factors have a negative impact on the work of all organs and systems, including reproductive, as well as significantly reduce the quality and life expectancy of dogs.

Effects of pollen concentration on allergic rhinitis in children: A retrospective study from Beijing, a Chinese megacity

With global climate change and rapid urbanization, the prevalence of allergic diseases caused by pollen is rising dramatically worldwide with unprecedented complexity and severity, especially for children in mega-cities. However, because of the lack of long time-series pollen concentrations data, the accurate evaluation of the impact of pollen on allergic rhinitis (AR) was scarce in the Chinese metropolis. A generalized additive model was used to assess the effect of pollen concentration on pediatric AR outpatient visits in Beijing from 2014 to 2019. A stratified analysis of 10 pollen species and age-gender-specific groups was also conducted during the spring and summer-autumn peak pollen periods separately. Positive associations between pollen concentration and pediatric AR varied with the season and pollen species were detected. Although the average daily pollen concentration is higher during the spring tree pollen peak, the influence was stronger at the summer-autumn weed pollen peak with the maximum relative risk 1.010 (95% CI 1.009, 1.011), which was higher than the greatest relative risk, 1.003 (95% CI 1.002, 1.004) in the spring peak. The significant adverse effects can be sustained to lag10 during the study period, and longer in the summer-autumn peak (lag13) than in the spring peak (lag8). There are thresholds for the health effects and they varied between seasons. The significant effect appeared when the pollen concentration was higher than 3.74 × 105 grain·m−2·d−1 during the spring tree pollen peaks and 4.70 × 104 grain·m−2·d−1 during the summer-autumn weed pollen peaks. The stratified results suggested that the species-specific effects were heterogeneous. It further highlights that enough attention should be paid to the problem of pollen allergy in children, especially school-aged children aged 7–18 years and weed pollen in the summer-autumn peak pollen period. These findings provide a more accurate reference for the rational coordination of medical resources and improvement of public health.

Feeding of walleye pollock and its feeding base in the Chukchi Sea in summer-autumn period

Data on feeding of walleye pollock <i>Gadus chalcogrammus</i> (3–70 cm long) in the Chukchi Sea in summer-autumn of 2017–2020 are presented. The food base includes zooplankton, benthic invertebrates and fish. The juveniles in the northwestern part of the sea consumed 0.033 t/km2 of prey per day, mainly fish (30.9 %), mysids (20.1 %) and amphipods (13.9 %), whereas the portion of copepods and euphausiids was 5.0–7.6 % of the diet. In the southwestern part of the sea, the juveniles consumption was much higher, as 0.200 t/km2 of prey per day, and included mainly euphausiids and copepods (24.9–51.1%). The adults with length 40–70 cm long dominated in the southwestern Chukchi Sea and consumed on average 13.2 t/km2 of prey per day, shared between fish (42 %), euphausiids (20 %), copepods (6 %), and decapods (11 %). Composition and structure of plankton was different between the main water masses on the shelf occupied the northern and the southern parts of the sea. In the northwestern Chukchi Sea, zooplankton distributed with the mean spatial density of 56.3 t/km2, formed mostly by chaetognaths (35.3 t/km2) and copepods (12.6 t/km2). In the southwestern Chukchi Sea, the mean spatial density of zooplankton was 49.2 t/km2, mostly euphausiids (17.3 t/km2), chaetognaths (15.8 t/km2) and copepods (9.2 t/km2). The total density was close to the level observed in the 2000s, but slight decreasing in biomass of chaetognaths and copepods was observed, mainly due to the recent lack of cold-water species <i>Calanus glacialis</i>, whereas the biomass of euphausiids, in particularly <i>Thysanoessa inermis</i>, had almost doubled. Biomass of the main groups of zooplankton prevailing in the diet in the Chukchi Sea in summer-autumn, as copepods and euphausiids, exceeded daily consumption of these groups by walleye pollock in 6–11 times that may indicate a sufficient food supply for this species. Besides, walleye pollock dwelled at the sea bottom and fed on shrimps, gammarids, bottom polychaetes, echiurids, mollusks, and also bottom fish. Total fullness of the pollock stomachs was in the range of 218–228 ‱ that also suggested successful feeding.

Phospholipid Composition of Fingerlings of Atlantic Salmon Salmo salar during Growth and Development in Aquaculture: The Effect of Different Lighting and Feeding Regimes.

The effect of different feeding and lighting regimes (natural and continuous) on the phospholipid composition of Atlantic salmon fingerlings reared in commercial aquaculture in the summer-autumn period in North Ossetia-Alania was studied. Qualitative and quantitative determination of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, lysophosphatidylcholine, sphingomyelin was performed by high-performance liquid chromatography. A decrease (September-November) in the content of the studied phospholipids in fingerlings decreased, which should be considered primarily as a biochemical adaptation of development and preparation of juveniles for the upcoming smoltification. The effects of lighting and feeding regime on phospholipid composition were found mainly in the fish reared under constant lighting and 24/7 feeding and the fish reared under natural light and feeding during daylight hours. However, the observed changes were not specific to a particular experimental group of fish in the framework of this study.

DYNAMICS OF PERMAFROST HEAVING HILLS IN THE SOUTHERN PERMAFROST ZONE OF WESTERN SIBERIA

The article presents an analysis of the annual dynamics of the temperature regime of the permafrost heaving mound in the southern permafrost zone of the West Siberian Plain. The study area belongs to the Tagrinskoye oil and gas field. The use in the study of the data of the automatic measuring system of temperatures within the heaving mounds made it possible to record the following parameters in time: temperature and moisture of the air at an altitude of 2 m, temperature and moisture of the soil at different depths. Modern automatic systems that record temperature data in permafrost make it possible to monitor changes in permafrost processes and predict undesirable technogenic hazards. The temperature regime of the permafrost heaving mounds is differentiated into an active layer with the dynamics of positive and negative temperatures and into the core of the heaving mounds, where long-term negative temperatures remain. Temperature study in permafrost is an important engineering and geological data that allows assessing the plasticity of permafrost in heaving mounds. It was determined that the active layer at a depth of 0,3-0,6 m has the highest dynamics of thawing and freezing, which refers to seasonal thawing. Permafrost rocks are plastic-frozen rocks, since at a depth of 1,6 m the temperature of permafrost increased to -0,3 °C and at a depth of 3 m it is underlain by melted sand with an average temperature of 0,66 °C. The dynamics of geomorphological changes depends on a complex of factors: the amount of precipitation in the summer-autumn period, the transition to zero temperatures and the amount of snow in the autumn-winter period.

The using of data on the moult progress in the study of migration periods of the annual cycle of passerines

Using trapping and ringing of birds for studying the ecology and the demographic parameters of populations is necessary to determine as accurately as possible in which period of the annual cycle each individual was caught. The first-year passerine birds undergo juvenile migration (usually in form of postfledging dispersal) and postmoulting (autumn) migration in summer-autumn period. These stages of annual cycle are difficult to identify in trapping birds by calendar dates because the timing of migration seasons can significantly overlap in the same location. The study of moult at the Ladoga Ornithological Station made it possible to determine the states of plumage that mark juvenile and postmoulting migrations in first-year birds. The article presents two examples of the use of moult progress in the study of bird migrations. The first one is an analysis of the ratio of the terms of departure and passage in three long-distant and three short-distant migrants of passerines. It has been shown that the timing of the departure of local birds is earlier than the timing of the passage of other populations in all studied species, while the last local individuals in long-distance migrants are recorded long before the end of the passage of the species, and in short-distance migrants they can be found until its end. As another example, an analysis of long-term trends in the timing of summer-autumn movements in the Garden Warbler was performed. It is shown that for almost a fifty-year observation period, neither the timing of juvenile migration nor the timing of postmoulting migration of first-year birds has changed. At the same time, the timing of the spring passage of the species shifted to earlier. As a result, the duration of stay of the Garden Warbler in the Ladoga region has increased.

Parameters of Lipid Metabolism in Underyearlings of Atlantic Salmon <i>Salmo salar</i> Reared under Different Regimes of Photoperiod and Feeding Modes in Aquaculture in the Sourthern Region of Russia

A comparative study of the lipid and fatty acid composition of Atlantic salmon underyearlings reared under natural and continuous light in commercial aquaculture in the summer-autumn period in North Ossetia-Alania was carried out. A stable change in the main indicators of lipid metabolism in salmon underyearlings from September to November was found, aimed at preparing juveniles for smoltification. A change in key fatty acid indicators was detected: an increase in the content of PUFAs due to (n-3) PUFAs, and the “marine” type of PUFA – 22:6 (n-3), high values of the ratios (n-3)(n-6) PUFA, 18:3(n-3)/18:2 (n-6), 22:6(n-3)/ 18:3(n-3). It has been shown the stimulating effect of the combined and stable action of the continuous photoperiod and feeding in the summer-autumn period on the preparation for smoltification of Atlantic salmon fingerlings grown under aquaculture conditions.

Stochastic Parameters of Flash Floods Formation in the North of the Black Sea Coast

Abstract—Flash floods are one of the most dangerous hydrometeorological events all over the World. In the current paper stochastic parameters of flash floods formation are studied on the basis of data on flash floods in 1990–2021 in the small river basins of the Caucasus and Crimea Black Sea Coast. The main factor of flash floods formation is heavy rain, but in some cases its occurrence could depend on critical combination of various factors. Flash floods are usually formed in summer-autumn period in the studied region with the maximum of observed events in August. They are characterised by very rapid water level rise of about 1.2–1.3 m/h. Sediment yield during one flash flood could be compared with mean annual values. Statistical analysis of precipitation long ranges demonstrates probability of more often flash floods occurrence in the region in comparison with observed events.

PHOSPHOLIPID COMPOSITION OF FINGERLINGS OF ATLANTIC SALMON <i>SALMO SALAR</i> DURING GROWTH AND DEVELOPMENT IN AQUACULTURE: THE EFFECT OF DIFFERENT LIGHTING AND FEEDING REGIMES

The effect of different modes of feeding and lighting (natural and continuous) on the phospholipid composition of Atlantic salmon fingerlings reared under commercial aquaculture in the summer-autumn period in North Ossetia-Alania was studied. Qualitative and quantitative determination of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, lysophosphatidylcholine, sphingomyelin was carried out by high performance liquid chromatography. A decrease (September-November) The content of the studied phospholipids in fingerlings decreased, which should be considered primarily as a biochemical adaptation of development, preparation of juveniles for the upcoming smoltification. The effects of lighting and feeding regime on phospholipid composition were found mainly in fish reared under constant lighting and 24/7 feeding and fish reared under natural light and feeding during daylight hours, however, the observed changes were not specific to a particular experimental group of fish in the framework of this study.

Feeding Features of the Nine-Spined Stickleback Pungitius pungitius (Gasterosteidae) In Water Bodies of the North-West of Russia

The diet of the nine-spined stickleback Pungitius pungitius has been studied in two water bodies in the north-west of Russia: in the subarctic lake Krivoe (Karelia) and the estuary of the Neva River (Leningrad Oblast). Diptera larvae (Chironomidae, Ceratopogonidae, Simuliidae, and Limoniidae) and planktonic crustaceans formed the basis of the food bolus of fish; however, their abundance and weight ratios differed in the studied water bodies. The seasonal changes of the dominant groups of food objects of the stickleback of Lake Krivoe have been revealed. Additionally to invertebrates and fish eggs, which form the basis of the stickleback diet in winter season, aquatic mosses as well as filamentous and diatom algae present in the fish diet in summer. The comparison of the nitrogen isotope composition of the muscles of stickleback, perch, and invertebrates shows that the stickleback of Lake Krivoe occupies the third trophic level, approaching predatory fish in δ15N values (5.2–8.6‰). A specific feature of the diet patterns of P. pungitius is the transition from a diverse diet of the summer–autumn period to a monotrophic animal diet (chironomid larvae and fish eggs) of the under-ice winter–spring period.

Littoral chironomids and oligochaetes in the subalpine Lake Maggiore: a first dataset

A dataset of 227 oligochaetes and 373 chironomids occurrence records from the subalpine Lake Maggiore, a large and deep temperate lake in Northern-Western Italy and Switzerland was developed within the Interreg Italy-Switzerland 2014-2020 Parchi Verbano Ticino Project (ID:481668) funded by the European Regional Development Fund (ERDF). The lake belongs to the national (LTER-Italy), European (LTER-Europe) and International (ILTER) long-term ecological research networks. Data were collected during the summer-autumn period in 2019-2021. Chironomids (Insecta, Diptera) and oligochaetes (Annelida, Clitellata) were identified to genus/species gr./species level by the authors. All 600 occurrence records are georeferenced and organised in a standardised Darwin Core Archive format. These data gathered along the littoral areas of Lake Maggiore will contribute to the development of common implementation strategies for shared and sustainable water management level of the lake, with particular reference to the protected natural areas (sites belonging to Natura 2000 network in Italy and to the Emerald Network in Switzerland). The authors strongly believe in the great potential of open access occurrence records in biogeographical studies and ecological research in the context of global environmental changes. For that reason, the dataset has been uploaded to the Global Biodiversity Information Facility (GBIF), an intergovernmental free and open access biodiversity data infrastructure.

Conservation of cholera vibrios in complex microcosm containing green microalgae

BACKGROUND: In the summer-autumn period, the peak of phytoplankton reproduction and flowering occurs and results in the deterioration of the quality of river water is noted. At the same time, cases of gastrointestinal diseases in humans are recorded, and epidemic complications of cholera may occur in endemic areas. V. cholerae form biofilm on the surface of chitinous hydrobionts and plastic components can lead to the spread of V. cholerae, possibly explaining the autochthonous mechanism of their existence in water bodies.
 AIM: This study determines the duration of Vibrio cholerae preservation on biotic (chitin) and abiotic (plastic) substrates in the presence of green unicellular algae with a change in cultivation temperature under experimental conditions.
 MATERIALS AND METHODS: This study used bacteriological and molecular genetic methods to achieve its goal.
 RESULTS: The preservation of toxigenic and non-toxigenic strains of V. cholerae O1 El Tor and V. cholerae O139 (ctxАВ+tcpА+csh1 and ctxАtcpАcsh1+) for six months, including three months at a low temperature simulating the autumn-winter period, as part of biofilms in microcosms, where one component is green microalgae. We noted an excess of the concentration of V. cholerae O1 and O139 serogroups by two orders of magnitude in samples where one of the components is chitin and the reproduction of green microalgae in the presence of a chitin substrate. This is probably one stage in the food chain in the ecology of water bodies and, accordingly, can be a reservoir for biofilm forms of cholera vibrios. Preservation of V. cholerae O1 El Tor non-toxigenic strain in a viable state in biofilm samples on plastic at decreased temperatures 82C for three months is possibly due to the presence of a cold shock gene in its genome.
 CONCLUSION: Without a substrate for adhesion, colonization, and biofilm formation, vibrios are incapable of long-term persistence at low temperatures.

Biochemical composition and antioxidant activity of broccoli (Brassica oleracea L. var. italica) during the summer-autumn cultivation period in South Dagestan

Broccoli cabbage Brassica oleracea L. var. italica is an economically important and widely cultivated vegetable crop, consumed both fresh and processed. To solve the problems of import substitution, the provision of high-quality varietal diversity of broccoli for agricultural producers in the middle zone of Russia and the Southern Federal District (SFD) comes first. Our work presents the results of the study of seven varieties and hybrids of broccoli, previously identified by productivity and commercial qualities. For the first time in the conditions of the northern dry subtropics of southern Dagestan, the content of dry matter, water-soluble polysaccharides, organic acids, flavonoids and antioxidant activity in fresh products were determined. The distribution of these substances by plant organs is shown. Broccoli samples with a high content of individual chemicals, as well as a set of characteristics that can be used as sources in breeding for an increased content of dry matter, water-soluble polysaccharides, organic acids, flavonoids and with high antioxidant activity, were isolated.