Abstract Horseshoe crabs, often known as Belangkas or Mimi, is one of the ancient marine faunas with protected conservation status. Horseshoe crabs are distributed in coastal waters of Indonesia of the islands of Sumatra, Java, Kalimantan, and Sulawesi, which are often caught as bycatch. Horseshoe crabs’s bloods were uniquewhich contain amebocytes as a test material for the presence of endotoxin bacteria. The purpose of this study was to identify the species of horseshoe crabs and to analyze environmental conditions, as well as the distribution of horseshoe crabs in the coastal waters of Pamekasan, Madura Island. This study was conducted in June 2023 by collecting data from crab fishers. ArcGIS was applied to delineate the distribution of horseshoe crab. The identification results showed that there was one species horseshoe crab of the Tachypleus gigas in the waters of Polagan Village, Galis District, Pamekasan Regency. The environmental condition of the waters in Polagan Village, Galis District, and Pamekasan Regency was relatively good. Habitat mapping is one way to protect these animals from the threat of extinction and support conservation efforts.
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