Attempts are going on to incorporate sulphur into the carbon and nitrogen removal processes at a biological wastewater treatment plant by external dosing or its intrinsic presence. A possible form of dosing sulphide at the field scale is the external dosing as the sodium salt of sulphide which under basic hydrolysis increases the pH. We demonstrated that the sodium salt dosage of sulphide can also be useful in achieving partial nitrification (PN). The results showed that 15 mgS/L of the sodium salt of sulphide was sufficient to establish PN in the low-strength synthetic and real wastewater under adequate dissolved oxygen concentrations (1.3 ± 0.4 mg/L during discharge phase of SBR cycle to 5.3 ± 0.2 mg/L during aeration phase of SBR cycle). PN was achieved only during the operational phases when the pH rise due to the basic hydrolysis of sodium sulphide was not controlled. The dosage of sulphide as its sodium salt has dual advantages as an inhibitor to nitrite oxidising bacteria (NOB). The activity of NOBs was 10–20 mgN/gVSS/d during the operational phases when PN was effective. The long-term operation showed a nitrite accumulation ratio of 70 ± 19% with an effluent ammonia concentration of 19 ± 4 mgN/L and nitrite concentrations of 18 ± 4 mgN/L. At the genus level, the NOB communities Nitrospira and Nitrobacter diminished in the long-term phases of stable PN with real wastewater. NOB communities evolved again when sulphide dosage was stopped indicating this strategy needs to be adopted continuously to achieve PN not time-based. The study demonstrated a strategy to achieve the feasibility of sulfide-driven PN systems and has a potential application in nitrogen removal from domestic wastewater.