Sugarcane Mosaic Virus Research Articles

Registration of ‘CP 14‐1490’ sugarcane

Abstract‘CP 14‐1490’ (Reg. no. CV‐212, PI 700785), a sugarcane (a complex hybrid of Saccharum spp) cultivar, was developed through cooperative research conducted by the USDA‐ARS, the University of Florida, and the Florida Sugar Cane League, Inc. The Florida Sugarcane Variety Committee released CP 14‐1490 in June 2021 to growers for commercial cultivation on organic (muck) soils. It originated from a cross made at Canal Point (CP) on 29 Nov. 2011, where ‘CP 04‐1844’ (PI 664935) was the female parent, and the male parent was ‘CPCL 02‐8021’. CP 14‐1490 was released for its high sugar yields; acceptable commercial recoverable sucrose (though it had significantly lower cane yield at the second ratoon); resistances to Sugarcane mosaic virus strain E (mosaic) and ratoon stunt disease (caused by Clavibacter xyli subsp. xyli Davis et al.); moderate resistances to brown [caused by Puccinia melanocephala (H. & P. Sydow)] and orange rusts [caused by Puccinia kuehnii (Kruger) E. Butler], and leaf scald [caused by Xanthomonas albilineans (Ashby) Dowson]; and moderate susceptibility to smut [caused by Sporisorium scitamineum (Syd., M. Piepenbr., M. Stoll & Oberw)]. Yield data of CP 14‐1490 and reference checks (i.e., ‘CP 00‐1101’ [PI 651881], ‘CP 96‐1252’ [PI 634935], ‘CPCL 05‐1201’ [PI 672485]) were collected from 15 harvests (i.e., three crop cycles [plant cane, first and second ratoon] at five organic‐soil locations in final‐stage replicated yield trials. There was no significant difference in cane yield with all tested reference checks and no significant difference in sugar yield with CP 00‐1101 and CPCL 05‐1201. When compared to CP 96‐1252, CP 14‐1490 had significantly (P ≤ .05) higher sucrose yield (19.2%) and commercial recoverable sucrose (5%). CP 14‐1490 rated moderately tolerant to freezing among 23 tested genotypes.

Registration of ‘CP 14‐1934’ sugarcane for Florida organic soils

Abstract‘CP 14‐1934’ (Reg. no. CV‐210, PI 700310) sugarcane cultivar, a complex hybrid of Saccharum spp., was developed through cooperative research of the USDA‐ARS, the University of Florida, and the Florida Sugarcane League. The Florida Sugarcane Variety Committee released CP 14‐1934 in June 2021 for commercial cultivation on organic (muck) soil. CP 14‐1934 originates from a cross CPX11‐1041 between CP 07‐2460 (female parent) and CP 06‐2335 (male parent). CP 14‐1934 was released due to its high cane yield; high sucrose yields; acceptable commercial recoverable sucrose; and resistance to brown rust (tested positive to Bru1 marker), orange rust, sugarcane mosaic virus strain E (mosaic), and ratoon stunting disease. CP 14‐1934 was found moderately resistant to leaf scald and to have intermediate freeze tolerance. Based on results of three harvests cycles over five locations in final‐stage replicated yield trials (Stage 4) on muck soils, CP 14‐1934 cane and sucrose yields were 17.8 and 19.8% greater than the mean of reference cultivars, respectively. CP 14‐1934 rarely flowers in Florida's climate. The new released cultivar CP 14‐1934 is expected to increase the profitability and the efficiency in the use of resources of Florida sugarcane growers.

Physical Properties of Potyvirus on Chilli (Capsicum annuum) of Doon Valley in Uttarakhand

A virus is transmitted mechanically in the plant host. Potyviridae is a family of Potyvirus,consisting of a positive-sense RNA genome and filamentous particles. It has a single typeof protein coat. Mostly, this virus is identified in Leguminosae and Solanaceae family. Theplant virus is transmitted by mechanical inoculation and aphids. Chilli, cucurbits, potatoes,peas, tomatoes, and other plants were infected with the bean yellow mosaic virus, pea mosaicvirus, bean yellow mosaic virus, sugarcane mosaic virus, and peanut mosaic virus. Thermalinactivation point, dilution endpoint, longevity in vitro, and longevity in vivo were used toidentify the physical properties of a potyvirus. Several isolates mild and severe were collectedin the chilli plant. The infected plant developed necrotic leaf spots and chlorotic symptoms.

Frontline Warrior microRNA167: A Battle of Survival

Plant pathogens such as viruses are detrimental to the survivorship of plant species. Coinfection of maize chlorotic mottle virus (MCMV) and the sugarcane mosaic virus (SCMV) causes a deadly disease in maize. An investigation by Liu et al. (2022) showed the role of Zma-miR167 in positively imparting resistance against the MCMV and SCMV. The authors identified ZmARF3 and ZmARF30 as the targets of Zma-miR167. ZmARF3 and ZmARF30 were identified as transcription factors that bind the cis-element in ZmPAO1 promoters to activate its expression. The authors showed how the Zma-miR167-ZmARF3/30-ZmPAO1 module functions differently in resistant and susceptible lines with high expression of Zma-miR167 in resistant lines correlated with the resistant phenotype. Finally, the authors concluded that MCMV-encoded p31 protein enhances ZmPAO1 enzyme activity for its survival in the host.

Cryobiotechnological approaches to eliminate Sugarcane mosaic virus (SCMV) in sugarcane (Saccharum spp. l.) infected plants

Cryobiotechnological approaches to eliminate Sugarcane mosaic virus (SCMV) in sugarcane (Saccharum spp. l.) infected plants

Registration of ‘CP 13‐4474’ sugarcane for sand soils

Abstract‘CP 13‐4474’ (Reg. no. CV‐209, PI 700305) sugarcane cultivar (a complex hybrid of Saccharum spp.) was released to growers in June 2021 for cultivating on sand soils in Florida after a decade‐long advancement through cooperative research conducted by the USDA‐ARS, the Florida Sugar Cane League Inc., and the University of Florida. It originated from a poly‐cross at Canal Point (CP). The Florida Sugarcane Variety Committee released CP 13‐4474 due to its high sugar yields, sucrose content on sand soils, and acceptable disease resistance. CP 13‐4774 has demonstrated resistance to brown rust (caused by Puccinia melanocephala H. & P. Sydow), orange rust (caused by Puccinia kuehnii E.J. Butler), mosaic (caused by Sugarcane mosaic virus strain E), and ratoon stunt (caused by Leifsonia xyli subsp. xyli Evtushenko et al.) and moderate resistance to smut [caused by Sporisorium scitamineum (Syd.) M. Piepenbr. et al.], leaf scald (caused by Xanthomonas albilineans Ashby, Dowson), and yellow leaf syndrome (caused by Sugarcane yellow leaf virus). In late‐stage yield trials, CP 13‐4474 sugar yields were 9.4% greater than that of the check variety ‘CL 88‐4730’, while sucrose yields did not differ significantly from the reference check ‘CP 96‐1252’. CP 13‐4474 displayed superior resistance to both brown and orange rusts and produced more commercial recoverable sucrose than all three tested reference checks over the same harvest trials. With its demonstrated resistance to diseases and elevated sugar production capacity, CP 13‐4474 is expected to positively influence both sugarcane production and crop sustainability in Florida.

Genetic diversity and molecular evolution of sugarcane mosaic virus, comparing whole genome and coat protein sequence phylogenies

Sugarcane mosaic virus (SCMV) is a widely distributed potyvirus that causes mosaic disease in sugarcane, maize, sorghum, canna, and other monocot species worldwide. This study used 139 SCMV full-length genome sequences to analyze the phylogenetic relatedness of geographically distinct isolates. The phylogenetic analysis revealed four major groups of SCMV isolates that relate to their primary host. The geographic locations for some isolates appear to be mismatched within the tree, suggesting either that convergent molecular evolution has occurred or that the tree reconstruction produces statistically significant incongruences that create uncertainty in the true evolutionary relationships of these virus isolates. Recombination analysis showed hot spots across most of the genome except in the coat protein (CP) coding region. We examined 161 SCMV CP sequences from the GenBank database, including sequences from samples collected in Pakistan, a region that has not been included in prior phylogenetic studies. These data suggest that the SCMV isolates from sugarcane (Saccharum officinarum) predate isolates from all other hosts, regardless of their geographic origins.

Differential Expression of Genes between a Tolerant and a Susceptible Maize Line in Response to a Sugarcane Mosaic Virus Infection

To uncover novel genes associated with the Sugarcane mosaic virus (SCMV) response, we used RNA-Seq data to analyze differentially expressed genes (DEGs) and transcript expression pattern clusters between a tolerant/resistant (CI-RL1) and a susceptible (B73) line, in addition to the F1 progeny (CI-RL1xB73). A Gene Ontology (GO) enrichment of DEGs led us to propose three genes possibly associated with the CI-RL1 response: a heat shock 90-2 protein and two ABC transporters. Through a clustering analysis of the transcript expression patterns (CTEPs), we identified two genes putatively involved in viral systemic spread: the maize homologs to the PIEZO channel (ZmPiezo) and to the Potyvirus VPg Interacting Protein 1 (ZmPVIP1). We also observed the complex behavior of the maize eukaryotic factors ZmeIF4E and Zm-elfa (involved in translation), homologs to eIF4E and eEF1α in A. thaliana. Together, the DEG and CTEPs results lead us to suggest that the tolerant/resistant CI-RL1 response to the SCMV encompasses the action of diverse genes and, for the first time, that maize translation factors are associated with viral interaction.

Supplementary information for The Potential Role of Translation Factors, Heat Shock Protein 90-2, and ABC Transporters in a Tolerant/Resistant Maize Line in Response to Sugarcane Mosaic Virus

Supplementary information for The Potential Role of Translation Factors, Heat Shock Protein 90-2, and ABC Transporters in a Tolerant/Resistant Maize Line in Response to Sugarcane Mosaic Virus

Maize resistance to insect herbivory is enhanced by silencing expression of genes for jasmonate‐isoleucine degradation using sugarcane mosaic virus

AbstractPreviously, sugarcane mosaic virus (SCMV) was developed as a vector for transient expression of heterologous genes in Zea mays (maize). Here, we show that SCMV can also be applied for virus‐induced gene silencing (VIGS) of endogenous maize genes. Comparison of sense and antisense VIGS constructs targeting maize phytoene desaturase (PDS) showed that antisense constructs resulted in a greater reduction in gene expression. In a time course of gene expression after infection with VIGS constructs targeting PDS, lesion mimic 22 (Les22), and Iodent japonica 1 (Ij1), efficient expression silencing was observed 2, 3, and 4 weeks after infection with SCMV. However, at Week 5, expression of Les22 and Ij1 was no longer significantly reduced compared with control plants. The defense signaling molecule jasmonate‐isoleucine (JA‐Ile) can be inactivated by 12C‐hydroxylation and hydrolysis, and knockout of these genes leads to herbivore resistance. JA‐Ile hydroxylases and hydrolases have been investigated in Arabidopsis, rice, and Nicotiana attenuata. To determine whether the maize homologs of these genes function in plant defense, we silenced expression of ZmCYP94B1 (predicted JA‐Ile hydroxylase) and ZmJIH1 (predicted JA‐Ile hydrolase) by VIGS with SCMV, which resulted in elevated expression of two defense‐related genes, Maize Proteinase Inhibitor (MPI) and Ribosome Inactivating Protein 2 (RIP2). Although ZmCYP94B1 and ZmJIH1 gene expression silencing increased resistance to Spodoptera frugiperda (fall armyworm), Schistocerca americana (American birdwing grasshopper), and Rhopalosiphum maidis (corn leaf aphid), there was no additive effect from silencing the expression of both genes. Further work will be required to determine the more precise functions of these enzymes in regulating maize defenses.

Complete genome sequence of a novel potyvirus infecting Miscanthus sinensis (silver grass)

Here, we describe the full-length genome sequence of a novel potyvirus, tentatively named “Miscanthus sinensis mosaic virus” (MsiMV), isolated from Miscanthus sinensis (silver grass) held in a post-entry quarantine facility after being imported into Western Australia, Australia. The MsiMV genome is 9604 nucleotides (nt) in length, encoding a 3071-amino-acid (aa) polyprotein with conserved sequence motifs. The MsiMV genome is most closely related to that of sorghum mosaic virus (SrMV), with 74% nt and 78.5% aa sequence identity to the SrMV polyprotein region. Phylogenetic analysis based on the polyprotein grouped MsiMV with SrMV, sugarcane mosaic virus (SCMV), and maize dwarf mosaic virus (MDMV). This is the first report of a novel monopartite ssRNA virus in Miscanthus sinensis related to members of the genus Potyvirus in the family Potyviridae.

Increasing risk of aphids spreading plant viruses in maize fields on both sides of China's Heihe-Tengchong line under climate change.

In the coming decades, geographical distribution patterns of farmland organisms may undergo drastic changes due to climate change, with significant implications for global food security. In China, Rhopalosiphum maidis and its spread of sugarcane mosaic virus (ScMV) can become an increasingly serious threat to maize (Zea mays) production. We conducted ecological niche modeling for Z. mays, R. maidis, and ScMV under current and future (2041-2060 and 2081-2100) climate scenarios by using MaxEnt software to explore changes in this system. The Heihe-Tengchong line (an imaginary separation line of human population density) can divide China into main (east of the line) and secondary (west of the line) habitats for the three species. With climate change, rapid expansion in suitable areas is projected for ScMV and the aphid vector R. maidis. Taking species interactions into consideration, our overlaying analyses show that most areas east of the Heihe-Tengchong line (optimal for maize and suitable for R. maidis) will become increasingly highly suitable for ScMV, suggesting that the prevention and control of this plant virus and its aphid vector in China's main maize-growing areas (e.g. northeast) will become an increasing challenge in the future. Climate change will profoundly affect ScMV-vector-maize interactions, which may contribute favorably to invasion of this virus into new areas. Our comprehensive and in-depth analyses on shifts in this multi-species system under climate change provide useful and insightful information for devising strategies for the prevention and control of plant viruses and aphid vectors on maize in the future. © 2022 Society of Chemical Industry.

Incidence and detection of wilting pathogen and Sugarcane mosaic virus (SCMV) in Pakistan

AbstractSugarcane (Saccharum officinarum L.) is a perennial grass, and it is cultivated worldwide for sugar and bioethanol production. During 2020–2021, countrywide field surveys were conducted and indicated the incidence of numerous phytopathogens in sugarcane crop. Infected sugarcane plants showed stunted growth, wilting, leaf yellowing and mosaic pattern in major sugarcane growing provinces of Pakistan (Sindh, Punjab and Khyber Pakhtunkhwa). Diseased plants were collected for the isolation of diseases‐causing pathogen. Wilted canes were cut and placed on potato dextrose agar (PDA) media, and after few days, pathogen was successfully grown on media. Based on fungal morphology and DNA sequence analysis, it was characterized and identified as Fusarium sacchari. Diseased sugarcane samples, showing mosaic symptoms, were collected from 57 locations and were subjected to ELISA test. Out of 570 symptomatic samples, 40 samples were tested positive for Sugarcane mosaic virus (SCMV). These results revealed a higher disease incidence rate (7.01%) of SCMV. This is the first report of countrywide incidence of SCMV in Pakistan.

Marker-trait Association for Resistance to Sugarcane Mosaic Virus (SCMV) in a Sugarcane (Saccharum spp.) Panel

Marker-trait Association for Resistance to Sugarcane Mosaic Virus (SCMV) in a Sugarcane (Saccharum spp.) Panel

A maize triacylglycerol lipase inhibits sugarcane mosaic virus infection.

Triacylglycerol lipase (TGL) plays critical roles in providing energy for seed germination and plant development. However, the role of TGL in regulating plant virus infection is largely unknown. In this study, we adopted affinity purification coupled with mass spectrometry and identified that a maize (Zea mays) pathogenesis-related lipase protein Z. mays TGL (ZmTGL) interacted with helper component-proteinase (HC-Pro) of sugarcane mosaic virus (SCMV). Yeast two-hybrid, luciferase complementation imaging, and bimolecular fluorescence complementation assays confirmed that ZmTGL directly interacted with SCMV HC-Pro in vitro and in vivo. The 101-460 residues of SCMV HC-Pro were important for its interaction with ZmTGL. ZmTGL and SCMV HC-Pro co-localized at the mitochondria. Silencing of ZmTGL facilitated SCMV infection, and over-expression of ZmTGL reduced the RNA silencing suppression activity, most likely through reducing HC-Pro accumulation. Our results provided evidence that the lipase hydrolase activity of ZmTGL was associated with reducing HC-Pro accumulation, activation of salicylic acid (SA)-mediated defense response, and inhibition of SCMV infection. We show that ZmTGL inhibits SCMV infection by reducing HC-Pro accumulation and activating the SA pathway.

UAVs to Monitor and Manage Sugarcane: Integrative Review

Pilotless aircraft systems will reshape our critical thinking about agriculture. Furthermore, because they can drive a transformative precision and digital farming, we authoritatively review the contemporary academic literature on UAVs from every angle imaginable for remote sensing and on-field management, particularly for sugarcane. We focus our search on the period of 2016–2021 to refer to the broadest bibliometric collection, from the emergence of the term “UAV” in the typical literature on sugarcane to the latest year of complete publication. UAVs are capable of navigating throughout the field both autonomously and semi-autonomously at the control of an assistant operator. They prove useful to remotely capture the spatial-temporal variability with pinpoint accuracy. Thereby, they can enable the stakeholder to make early-stage decisions at the right time and place, whether for mapping, re-planting, or fertilizing areas producing feedstock for food and bioenergy. Most excitingly, they are flexible. Hence, we can strategically explore them to spray active ingredients and spread entomopathogenic bioagents (e.g., Cotesia flavipes and Thricrogramma spp.) onto the field wherever they need to be in order to suppress economically relevant pests (e.g., Diatraea saccharalis, Mahanarva fimbriolata, sugarcane mosaic virus, and weeds) more precisely and environmentally responsibly than what is possible with traditional approaches (without the need to heavily traffic and touch the object). Plainly, this means that insights into ramifications of our integrative review are timely. They will provide knowledge to progress the field’s prominence in operating flying machines to level up the cost-effectiveness of producing sugarcane towards solving the sector’s greatest challenges ahead, such as achieving food and energy security in order to thrive in an ever-challenging world.

Ultrasensitive nano-gold labelled, duplex lateral flow immunochromatographic assay for early detection of sugarcane mosaic viruses

Sugarcane is one of the important food and bioenergy crops, cultivated all over the world except European continent. Like many other crops, sugarcane production and quality are hampered by various plant pathogens, among them viruses that infect systemically and cause severe impact to cane growth. The viruses are efficiently managed by their elimination through tissue culture combined with molecular diagnostics, which could detect virus titre often low at 10–12 g mL−1. To harmonize the virus diagnostics by molecular methods, we established a nanocatalysis-based high sensitive lateral flow immunochromatographic assay (LFIA) simultaneously to detect two major sugarcane viruses associated with mosaic disease in sugarcane. LFIA is known for poor sensitivity and stability with its signalling conjugates. However, we synthesized positively charged Cysteamine-gold nanoparticles and used them to prepare highly stable to sensitive immunoconjugates and as a colourimetric detection label. Further nanogold signal enhancement was performed on LFIA to obtain a high detection sensitivity, which is higher than the conventional immunoassays. The linear detection range of the nano-LIFA was 10–6 to 10–9 g mL−1, and with the signal enhancement, the LOD reached up to 10–12 g ml−1. This research paper provides relative merits and advancement on nano-LFIA for specific detection of sugarcane viruses in sugarcane for the first time.

甘蔗类泛素蛋白UBL5应答SCMV侵染及其与SCMV-6K2的互作

<p id="C3">Ubiquitylation plays key roles in the regulation of protein function, growth and development, and response to stress. Ubiquitin-like proteins (UBLs) are the main components of the ubiquitin-proteasome system (UPS). In our previous study, the UBL5 homologue was isolated from sugarcane (<italic>Saccharum </italic>spp. hybrid) by yeast two-hybrid (Y2H) with the 6K2 of <italic>Sugarcane mosaic virus</italic> (SCMV) as bait, and then designated as ScUBL5 with 73 aa in length. In the present study, the interaction of ScUBL5 with the SCMV-6K2 was further confirmed by bimolecular fluorescence complementation assays (BiFC). Bioinformatics analysis showed that ScUBL5 is a stable hydrophilic non-secretory protein without signal peptide or transmembrane domain. Phylogenetic tree analysis showed that <italic>ScUBL5</italic> is species specific. Subcellular localization analysis showed that ScUBL5 is localized in cytoplasm and nucleus. <italic>ScUBL5</italic> gene shows obvious tissue specificity in sugarcane by real-time quantitative PCR analysis. The expression levels of ScUBL5 gene in the established morphogenesis tissues such as the 1st leaf, the 7th leaf, the 8th internode and the root were significantly higher than those in the immature tissues such as leaf roll and the 3rd internode. The expression of<italic> ScUBL5</italic> gene is significantly affected by SCMV infection. <italic>ScUBL5</italic> was significantly upregulated in the early stage of SCMV infection, then downregulated but significantly higher than the control at the late stage of SCMV infection.

Incidence and Distribution of Four Viruses Causing Diverse Mosaic Diseases of Sugarcane in China

Mosaic diseases of sugarcane caused by various viruses have been reported in most sugarcane planting countries and threaten global sugar production. There is a lack of extensive, systematic investigation of mosaic diseases and their causal viruses in China. In this study, a total of 901 leaf samples showing mosaic symptoms were collected from commercial fields in eight provincial regions in China and tested for sorghum mosaic virus (SrMV), sugarcane mosaic virus (SCMV), sugarcane streak mosaic virus (SCSMV), and maize yellow mosaic virus (MaYMV) using RT-PCR with four specific primer pairs. Of 901 tested samples, 38.5% (347/901) of samples were infected with one of the four viruses alone. Infection by two or more viruses was seen for 42.6% (384/901) of samples. The highest incidence of virus-causing sugarcane mosaic disease was SrMV (70.1%), followed by SCMV (33.4%) and SCSMV (30.3%), and the lowest incidence was seen for MaYMV (5.1%). Three viruses (SrMV, SCMV, and SCSMV) were found in eight sugarcane-planting provinces, whereas MaYMV was only found in Fujian, Guangxi, and Sichuan provinces. Mixed infections of the three main viruses, particularly for SrMV + SCMV and SrMV + SCSMV, were commonly found in the sugarcane samples. Our systematic determination of the occurrence and distribution of four RNA viruses associated with sugarcane mosaic diseases can provide evidence to guide the development of strategies for the prevention and control of sugarcane mosaic diseases in China.

Sugarcane mosaic virus (sugarcane mosaic)

Sugarcane mosaic virus (sugarcane mosaic)