This study was undertaken to evaluate the performance of cut Dendrobium pink sunshine under various holding solutions at post graduate lab Department of Horticulture, Faculty of Agriculture, Annamalai University, Tamil Nadu, India, during February 2016. The treatments with three replications were carried out in completely randomized design with 3 spikes in each replication. Nine chemical preservative solutions were used for extending the vase life and the treatments are T1 8-HQC (200-ppm), T2 Citric Acid (200-ppm), T3 STS (200-ppm), T4 GA (200-ppm), T5 Al2 (SO4)3 (10-ppm), T6 8-HQC (200-ppm) + 5% sucrose, T7 Citric Acid (200- ppm + 5% sucrose, T8 STS (200-ppm) + 5% sucrose, T9 GA (200-ppm) + 5% sucrose,T10 Al2(SO4)3 (200-ppm) + 5% sucrose and T11 Distilled water. Postharvest observations including bud open (%), flower drop (%), flower colour retention (days), flower diameter (cm), vase life and water uptake (ml). Among the treatments (T10) Al2(SO4)3 (200-ppm) + 5% sucrose recorded maximum performances in the postharvest studies followed by (T8) STS (200-ppm) + 5% sucrose. This study reports the phytochemical production from the callus and shoot cultures of Phyllanthus amars. Callus and shoot cultures were established from the nodal explants. Murashige and Skoog medium supplemented with N 6 - benzylaminopurine was found best medium for multiple shoot regeneration from the node explant and further maintenance of the shoot cultures. Callus induction was favoured on medium supplemented with 4.50 µM 2,4- dichlorophenoxy acetic acid. In vitro regenerated shoots and callus cultures were dried at room temperature and after measuring the dry biomass subjected for phytochemical extraction by using methanol and water. The extracts were quantified tested for total phenolics and flavonoids. The methanolic extract of in vitro and in vivo shoot showed the antioxidant activity.