Successful Growth Research Articles

Generation of bovine decellularized testicular bio-scaffolds as a 3D platform for testis bioengineering

Accelerating the genetic selection to obtain animals more resilient to climate changes, and with a lower environmental impact, would greatly benefit by a substantial shortening of the generation interval. One way to achieve this goal is to generate male gametes directly from embryos. However, spermatogenesis is a complex biological process that, at present, can be partially reproduced in vitro only in the mouse. The development of reliable 3D in vitro models able to mimic the architecture and the physiological microenvironment of the testis, represents a possible strategy to facilitate ex vivo haploid male gamete generation in domestic species. Here we describe the creation of bovine testicular bio-scaffolds and their successful repopulation in vitro with bovine testicular cells. In particular, bovine testes are subjected to three different decellularization protocols. Cellular compartment removal and extracellular matrix preservation are evaluated. The generated bio-scaffolds are then repopulated with bovine testicular fibroblasts. The results obtained demonstrate that the decellularization protocol involving the use of 0.3% sodium dodecyl sulfate (SDS) for 12 h efficiently eliminates native cells, while preserving intact ECM composition and microstructure. Its subsequent repopulation with bovine fibroblasts demonstrates successful cell homing, colonization and growth, consistent with the scaffold ability to sustain cell adherence and proliferation. Overall, the generated 3D bio-scaffolds may constitute a suitable artificial niche for ex vivo culture of testicular cells and may represent a possible strategy to reproduce spermatogenesis in vitro.

Research the Reasons for BiliBilis Success

BiliBili has grown into a successful commercialization and sustainable growth example in the digital l entertainment industry since its listing on NASDAQ in 2018. In this study an attempt has been made to examine the factors that have contributed to BiliBilis success, specifically, the companys unique business model, community centered approach, and e-sports initiatives. The paper went on to a deep analysis of BiliBili's diversity of offerings, including Professional User Generated Videos (PUGV), Occupationally Generated Videos (OGV) and its active user community consisting mainly of Generation Z. This paper identifies the mechanisms that have propelled Bilibili's consistent user engagement, and revenue growth, by evaluating the company's marketing strategies, its unique PUGV+OGV content model, and its content ecosystem. In addition, a SWOT analysis of BiliBili identifies the strengths of diversified monetization channels, as well as challenges and opportunities in the forming market. It turns out BiliBilis forray into e-sports is an area of untapped revenue that drives through innovative ways of using brand sponsorships, player endorsements, event hosting and content production all of which contribute big to its revenue. Besides the insights provided in the development trajectory of BiliBili itself, this study also represents valuable references to other emerging digital platforms pursuing sustainable business practices or community driven growth strategies.

An Analysis of Cash Management Strategies in E-Commerce Companies

This paper investigates the vital role of cash management in e-commerce companies, focusing on three major players: Pinduoduo, Jing Dong, and Amazon. The study delves into the strategies these firms use to optimize cash flows, emphasizing how technological advancements and social factors unique to the e-commerce industry influence their cash management practices. By exploring the advanced systems implemented by these companies, this research provides valuable insights into the techniques that drive financial efficiency. In addition to analyzing existing cash management methods, the study incorporates a comparative analysis of financial models and statistical data to highlight the best practices in the sector. The findings underscore the importance of cash management in sustaining operational success and long-term growth in the e-commerce industry. Furthermore, the paper offers predictions on future trends, identifying potential challenges and opportunities for innovation in cash management. This research aims to contribute to the broader understanding of financial optimization in online retail, offering actionable insights for both academics and practitioners in the field.

Precision targeting of fat metabolism in triple negative breast cancer with a biotinylated copolymer.

Mitochondrial CPT1-mediated fatty acid β-oxidation (FAO) critically contributes to the accelerated metastatic expansion of triple negative breast cancer (TNBC). Hence, inhibition of FAO through active CPT1 targeting could be a promising therapeutic approach in anti-TNBC therapies. Herein, we strategically synthesized a pyrene chain end labelled copolymer bearing biotin pendants, CP4, that actively targets CPT1 and efficiently blocks FAO in metastatic TNBC. Following the comprehensive characterization and synthesis of CP4, in silico negative docking score and Ramachandran plot analyses confirmed its on-target binding potential to CPT1. As a result, CP4 disrupts mitochondrial membrane potential, generates excessive ROS, and restricts excessive ATP production by impairing mitochondrial respiration, glycolytic function, and FAO. Subsequently, CP4 suppressed FA uptake and regulated FAO-associated gene expressions, exhibiting successive metastatic growth inhibition and apoptosis induction. Also, in an animal model, CP4 demonstrated active binding to CPT1, as evidenced by the significant depletion of CPT1A expression in tumor and liver tissue, akin to the specific CPT1-targeted drug. This active targeting of CPT1 has further consolidated the healing of altered lipid and oxidative stress, resulting in remarkable tumor regression, highlighting CP4 as a promising anticancer therapy focused on mitochondrial FAO, advancing future breast cancer treatments.

Establishment of a visualized mouse orthotopic xenograft model of nasopharyngeal carcinoma

ABSTRACT Mouse orthotopic xenograft tumor models are commonly employed in studies investigating the mechanisms underlying the development and progression of tumors and their preclinical treatment. However, the unavailability of mature and visualized orthotopic xenograft models of nasopharyngeal carcinoma limits the development of treatment strategies for this cancer. The aim of this study was to provide a simple and reliable method for building an orthotopic xenograft model of nasopharyngeal carcinoma. Human nasopharyngeal carcinoma (C666–1-luc) cells, stably expressing the firefly luciferase gene, were injected subcutaneously into the right axilla of BALB/C nude mice. Four weeks later, the resulting subcutaneous tumors were cut into small blocks and grafted into the nasopharynx of immunodeficient BALB/C nude mice to induce tumor formation. Tumor growth was monitored by bioluminescence imaging and small animal magnetic resonance imaging (MRI). The expression of histological and immunological antigens associated with orthotopic xenograft nasopharyngeal carcinoma was analyzed by tissue section analysis and immunohistochemistry (IHC). A visualized orthotopic xenograft nasopharyngeal carcinoma model was successfully developed in this study. Luminescence signal detection, micro-MRI, and hematoxylin and eosin staining revealed the successful growth of tumors in the nasopharynx of the nude mice. Moreover, IHC analysis detected cytokeratin (CK), CK5/6, P40, and P63 expression in the orthotopic tumors, consistent with the reported expression of these antigens in human nasopharyngeal tumors. This study established a reproducible, visual, and less lethal orthotopic xenograft model of nasopharyngeal carcinoma, providing a platform for preclinical research.

An Analysis Of Linguistic Errors In Scientific Articles Of Indonesian Language Education Students

This study examines linguistic errors in scientific articles written by students from the Indonesian Language Education Study Program at Universitas Malikussaleh. The primary objective is to identify, categorize, and analyze common mistakes that compromise the clarity, coherence, and quality of student writing. Employing a descriptive qualitative approach, the research focuses on errors in morphology, syntax, spelling, and punctuation. The data comprises four student-authored scientific articles published in Jurnal Kande, selected through purposive sampling based on criteria such as academic supervision and thematic relevance to Indonesian language education. The analysis reveals recurring issues, including grammatical inaccuracies, improper sentence structures, misused terminology, and spelling and punctuation errors, which diminish the clarity of arguments and affect the credibility of students' scientific communication. By identifying error patterns through content analysis, the study provides insights into their causes and effects, contributing to the existing knowledge of linguistic error analysis. The findings offer practical recommendations for enhancing students' scientific writing skills, which are essential for academic success and future professional growth. In conclusion, this study emphasizes the need for targeted linguistic training and mentorship to improve the quality of scientific writing and highlights the importance of continuous support systems in academic settings to address persistent language errors. It advocates for the implementation of specialized educational strategies to enhance students' writing clarity, accuracy, and professionalism.

Рост окуня Perca fluviatilis L., 1758 (Percidae) в казахстанской части бассейна Ишима

The growth of Eurasian perch in water bodies within the Ishim River system in the Republic of Kazakhstan has been investigated. Data from 29 samples from various water bodies of the Ishim watershed and some adjacent watersheds are presented. The most extensive age series were found in specimens from Lake Lobanovo (National Park “Kokshetau”) – up to sixteen years (15+). River populations had a shorter lifespan. Comparison of growth rate patterns indicates the presence of two conditional “units”: the first includes the majority of lake populations, while the second includes Karatomarskoe Reservoir and Lake Lobanovo. Some samples are located outside of these groupings. The examined chronological samples show significant differences in growth for some. This is typical for Lake Karakol and, partialy, – the Ishimskoe Reservoir, where positive hydrological changes occurred between perch catches years, resulting in increased growth rates. These samples exhibit a low level of correlation in growth indicators between adjacent ages, indicating disruption in growth rate “canalization” and the acquisition of opportunities for individuals in a renewed unbalanced habitat environment. In relatively stable conditions of most other water bodies, the correlation level of these data indicators is high for the majority of comparison pairs. When comparing Ishim populations with previously published data on the Nura and Sarysu river watersheds re-garding growth rates, one can empirically conclude about the more successful growth of perch in the Nura-Sarysu watershed. However, it should be noted that mainly water bodies without commercial fishing and with developed recreational fishing were studied in the Ishim watershed. In contrast, water bodies with predominant commercial fishing were surveyed in the Nura-Sarysu system. The different impacts of these types of fishing may determine both the growth rates and the lifespan of perch.

Peran Pelatihan dan Pengembangan Sumber Daya Manusia dalam Meningkatkan Service Quality dalam Kinerja Perusahaan

In the midst of increasingly fierce business competition, companies need to continuously improve their service quality in order to maintain customer loyalty and gain a larger market share. The role of human resources (HR) has a vital role in improving service quality to excel, especially in interacting with customers. In this article, titled “The Role of Human Resources Training and Development in Improving Service Quality in Company Performance,” a literature review has helped the researcher to frame an in-depth analysis based on existing data to understand the contribution of employee training and development in improving service quality in the company. In addition to improving individual competencies, HR training and development also plays an important role in shaping an organizational culture that prioritizes service quality. Thus, companies need to pay serious attention to human resource training in order to continue to develop, as this is an important step in achieving success and sustainable growth.

An assessment of the supply chain management for agricultural products in Cambodia

The primary objective of this research is to examine the supply chain management of cashew nuts, mangoes, and cassava in Cambodia. The findings indicate that Cambodian farmers predominantly rely on traditional methods for crop cultivation. A significant issue is their lack of adequate knowledge regarding the assessment and utilization of land quality, which results in both the quantity and quality of their crop yields falling short of acceptable standards. Consequently, this deficiency adversely affects the market prices of their produce. To address this knowledge gap, it is essential for relevant institutions, particularly the Ministry of Agriculture, Forestry and Fisheries, to offer training programs that focus on modern agricultural techniques aimed at enhancing both the quantity and quality of crop production. Additionally, the study highlights the absence of an institution dedicated to analyzing land types and quality in Cambodia. Moreover, the establishment of an effective irrigation system is crucial for the successful growth of crops.

Corrosion of nickel foam electrodes during hydrothermal reactions: The influence of a simple protective carbon black coating

Nickel foam (NF) substrates are widely used to support electrocatalysts, and this is frequently achieved using hydrothermal reactions, where the NF is immersed in the hydrothermal reactor together with the electrocatalyst precursors. However, other reactions including the corrosion of the NF and changes to the pH occur simultaneously, and these can affect the quality of the final electrocatalyst. Herein, a simple approach is devised to minimise these unwanted reactions. Carbon black (CB) was non-covalently functionalised at room temperature using tannic acid to give very stable and good dispersions of fCB in deionised water. Using a simple sonication step, the NF was coated with a uniform layer of the dispersed fCB. This layer served to minimise the corrosion of the underlying NF during the hydrothermal reactions with very good protection observed up to a temperature of 160 °C in deionised water at a pH of 2.0. The corrosion currents of the NF and fCB@NF were estimated at 8.7 µA and 3.9 µA, respectively, at room temperature in this acidic solution. Using a model reaction, the successful nucleation and growth of MnCo2O4 cubes was observed at fCB@NF, but not at the corroding NF.

Probing Surface-Mediated Electronic Coupling in Flat Hexagonal Phosphorus Nanostructures and Monolayer on Au(111).

Due to its diverse allotropes and intriguing properties, 2D phosphorus, also known as phosphorene, is a material of great interest. Here, the successful growth of flat hexagonal 2D phosphorus on Au(111) is reported. Starting from phosphorus linear chains at low coverage, a porous network and finally an extended 2D flat hexagonal (HexP) layer while increasing phosphorus deposition is formed. Using scanning tunneling microscopy/spectroscopy combined with ab initio calculations, the structure and electronic properties of the as-grown phosphorus structures are followed. The progressive formation of a phosphorus electronic band in the conduction band region is followed. More strikingly, a partial flatband that appears only on the HexP phase characterized by a sharp peak in the electronic spectrum is observed. These bands arise from the hybridization of phosphorus and gold atoms. This novel phosphorus-based structure exhibits remarkable electronic properties due to gold mediated phosphorus-phosphorus electronic coupling. This work paves the way for new interface material developments with attractive electronicproperties.

Impact of Marketing Strategies on Business Growth of Small and Medium Enterprises in Sri Lanka

This study was conducted to find the impact of marketing strategies on growth ofsmall and medium enterprises: special reference to Northern Province. Marketingstrategies consisted as marketing mix strategy, Ansoff matrix, and porter’s generictheory. The study was motivated by the fact that SMEs find themselves in acompetitive environment and may not know which marketing strategies are effectivein improving their business performance. The study adopted the survey methodamong 100 SMEs within the Northern Province. The data obtained was analyzedusing validity test, reliability test, descriptive analysis, correlation analysis andregression analysis with the use of Statistical Package for Social Science (SPSS). Thestudy revealed that there is a significant impact of marketing strategies on businessgrowth of SMEs. The study recommends that SMEs should concern more onmarketing mix strategy, Ansoff matrix and porter’s generic theory in marketingstrategies to improve the growth of the business. Findings showed that adjusted R2ofMarketing Strategies is 0.779. Therefore, 77.9% of variation in Business growthexplained by marketing strategies. This study facilitates to SMEs and policy makers toimplement efficient policies the successful business growth.

Selective area molecular beam epitaxial growth of n-GaN nanowires on SiO2 hole-mask patterned sapphire and graphene substrates

The growth conditions of silicon-doped GaN (n-GaN) nanowire (NW) selective area growth (SAG) are explored using plasma-assisted molecular beam epitaxy on SiO2 hole-mask patterned substrates: sapphire (SAP) and graphene on SAP (GoS). To achieve high selectivity, the growth temperature and Ga flux windows in which the nucleation on the SiO2 mask is highly suppressed are investigated and found to be quite narrow. By tuning the mask etching process, n-GaN NW morphologies ranging from vertical and widely separated NWs grown through SiO2 pinholes to densely positioned NWs with coalesced upper surface in wider mask holes are demonstrated on SAP.Using optimized growth conditions for SAG on SAP substrates, successful growth of vertical n-GaN NWs was achieved on similarly hole-mask patterned GoS substrates. Micro-Raman spectra indicate that the graphene is well protected from the nitrogen plasma by the SiO2 mask, whereas the graphene is damaged in the holes of the mask. X-ray diffraction spectra reveal an epitaxial relationship with the SAP and resistance measurements show conductivity between NWs of different mask holes. From this, we conclude that n-GaN NWs after nucleating on SAP through nanoholes in the graphene overgrow it and that this part of the graphene, at least close to the edge of the hole areas, can conduct well to the SiO2 mask covered graphene, indicating that graphene in such configuration can be used as a bottom electrode in a device structure. The impact of Ga diffusion and desorption on SAG is demonstrated for growth temperatures of 810 and 815 °C for SiO2 hole-mask patterns with nominal hole diameter of 2.5 µm and hole pitches of 3.0–10.0 µm. For hole pitches above 5 µm, Ga diffusion from the mask is the major contribution to the NW growth.

The Impact of Business Incubation Programmes on Start-up Success and Economic Growth in Indonesia

The Impact of Business Incubation Programmes on Start-up Success and Economic Growth in Indonesia

Continuous preparation of hydro-alcoholic gel with antimicrobial complex nanoparticles via cascaded reactive confined impinging jets micromixing

Hand hygiene was crucial for suppressing a spread of infectious diseases. A gel hand sanitizer (GHS) can be used in circumstances of a water shortage and suitable for outdoor usages. A hydro-alcoholic gel formation required mixing a viscous aqueous solution of a thickener with an alcohol solution of a gelling reagent. The homogeneity of the mixing and the gelling reaction dominantly affected the product quality and the expiration period. Moreover, a GHS was typically only able to kill the pathogens rather than also to inhibit a successive pathogen growth. To overcome this mixing issue as well as prolong antimicrobial persistence, this study employed a confined impinging jets (CIJ) microreactor to generate a GHS with suspending antimicrobial nanoparticles of carboxymethyl chitosan (CMC)/Zn(II)/proanthocyanidins (PAC) via the reactive flash nanoprecipitation (RFNP), intensifying shear thinning in turbulent mixing. The pressure drops and the energy consumption of the CIJ micromixing were theoretically analyzed. The developed process was continuous, effective and energy-saving and the device was compact but capable of massively producing the GHS, enabling a distributed production and beneficial to infection controls in undeveloped areas.

Unraveling the venom constituents of the endoparasitoid Aphidius gifuensis with an emphasis on the discovery of a novel insecticidal peptide.

Venom serves as a pivotal parasitic factor employed by parasitoid wasps to manipulate their hosts, creating a favorable environment for the successful growth of their progeny, and ultimately kill the host. The bioactive molecules within parasitoid venoms exhibit insecticidal activities with promising prospects for agricultural applications. However, knowledge regarding the venom components of parasitoids and the discovery of functional biomolecules from them remains limited. In this study, 30 venom proteins were identified from the endoparasitoid Aphidius gifuensis through the application of a transcriptomic approach. These proteins were categorized into five groups: hydrolase, molecular chaperone, transferase, other functional protein, and hypothetical protein with unknown function. Particularly noteworthy is the abundant expression of the peptide Vn1 in the venom apparatus of A. gifuensis, indicating its pivotal role in venom activity. Consequently, Vn1 was chosen for further functional analysis, exhibiting insecticidal activity against Tenebrio molitor pupae. Further assessment for revealing its mode of action disclosed that Vn1 impacts genes related to immune response, environmental information processing, metabolism, and response to external stimuli in T. molitor, suggesting its involvement in the intricate parasitoid wasp-host interaction. The findings of this study significantly contribute to our knowledge of the composition and functionality of A. gifuensis venom, establishing a foundation for further investigation into the biological roles of the identified venom constituents. The insecticidal Vn1 isolated from the venom of this parasitoid represents a valuable resource for the development of innovative biocontrol agents with potential applications in agriculture. © 2024 Society of Chemical Industry.

Level of Awareness of ICT Tools Among the Faculty Working in Higher Education Institutions in the Malabar Region, Kerala

Introduction: The advent of internet and innovative technologies has rendered learning solution for universities currently navigating an environment of passionate change. Universally, the COVID-19 lockdown has impacted every facet of life, and education is no exception. Understanding faculty perceptions on e-learning technologies is essential for the successful growth of academic programs. Objective: This study aims to investigate college faculty members current status and future expectations regarding e-learning. Method: It is an empirical research study encompassing faculty teaching higher education in arts, sciences, humanities, engineering, and health sciences. The purposive sampling, with a sample size of 389 is adopted for the study. The primary data is collected through questionnaire. Descriptive Statistics and Regression, aided by IBM SPSS Statistics 22, are employed for analysis and interpretation. Results: The study reveals a moderate awareness level towards E-Learning.

Deposition of SiC Thin Films By Using R. F. Power Ramps

SiC is a broadly researched and utilized material in a wide variety of known applications like coatings, MOSFETs and other miscellaneous devices; Regardless of this, SiC technology still has potential growth in new material engineering and devices through use of modern growth techniques and methods. In this study, we attempted to deposit SiC films on Si and glass substrates by using low temperature and different R. F. power ramps in a Magnetron Sputtering.The experiment presented successful growth of SiC thin films through a novel ramping method that allowed us to deposit a thin film at room temperature. The films were characterized by using AFM Microscopy, Spectroscopic Ellipsometry and Micro-Raman Spectroscopy. The valuable information obtained on surface morphology, band gap energy and crystal structure will fuel further endeavors to continually develop this material for future applications.AFM measurements presented a periodic grain pattern akin to terraces with quadrilateral grains and a meaningfully different geometry when compared to traditional semiconductor surfaces. Ellipsometry data provided a band gap energy from 3.3 eV. Raman spectra revealed broad peaks pertaining to multiple SiC polytypes.

HWCVD growth of hydrogenated nanocrystalline silicon oxide window layers for SHJ solar cells

In recent years, successful development of n-type hydrogenated nanocrystalline silicon oxide (n-nc-SiOx:H) window layers with high conductivity and low parasitic absorption, grown by plasma enhanced chemical vapor deposition (PECVD) technology, has significantly enhanced the power conversion efficiency of silicon heterojunction (SHJ) solar cells. Although SHJ solar cells have significant advantages in efficiency, the high cost of the PECVD equipment presents a significant barrier to their mass production. The utilization of the more cost-effective hot wire chemical vapor deposition (HWCVD) technology presents a promising solution. Nevertheless, HWCVD technology currently lacks an industrially viable methodology for growing high-performance window layers due to a phenomenon of associated severe loss of passivation. In this work, the phenomenon and the solution have been investigated. The results demonstrate that, in HWCVD processes, large amounts of hydrogen and small amounts of oxygen, which are essential for enhancing the crystallinity and conductivity and reducing the parasitic absorption, both induce significant damage to the previously grown intrinsic hydrogenated amorphous silicon layer, leading to poor passivation, and hence much lower cell efficiency. Introduction of a densified amorphous silicon barrier layer was found to be an effective solution to the aforementioned passivation loss, resulting in the successful growth of nc-SiOx:H window layers by HWCVD, with an efficiency enhancement of 0.37%abs. Finally, a commercial-size SHJ solar cell with an efficiency of 24.74 % was fabricated using a home-made HWCVD-based pilot SHJ cell line.

Crystal growth and pressure effect on the transport properties in 122-type (La,Na,K)Fe2As2 superconductors

Abstract High-quality single crystals are essential for probing the intrinsic properties of unconventional superconductors. In this work, we report the successful growth of (La,Na,K)Fe2As2 single crystals, a novel 122-type iron-based superconductor, using the self-flux method. The crystal structure and chemical composition were characterized through x-ray diffraction and energy-dispersive x-ray spectroscopy. The (La,Na,K)Fe2As2 single crystals exhibit bulk superconductivity, with a critical transition temperature Tc of approximately 22 K. Magnetization measurements reveal a second peak effect and a critical current density Jc ~ 5×105 A/cm2 at 2 K in a self-field. The upper critical field anisotropy was systematically studied within the framework of the anisotropic Ginzburg–Landau theory, yielding an anisotropy value of approximately 2.6. Furthermore, we employ the diamond anvil cell technique to investigate the pressure effect on (La,Na,K)Fe2As2. Our results demonstrate that superconductivity is gradually suppressed with increasing pressure, while the normal state resistivity at low temperatures evolves from non-Fermi liquid to Fermi liquid behavior. This observation suggests that (La,Na,K)Fe2As2 may be situated on the right of the quantum critical point or, at the very least, within the over-doped region. These findings provide a critical sample platform and experimental insights for advancing the understanding of the physical properties of the newly discovered (La,Na,K)Fe2As2 superconductors.