Utilizing potential evapotranspiration (PET) for the estimation of actual evapotranspiration (AET) in cropland is highly valuable for determining agricultural water requirements and developing irrigation schedules. Discrepancies between anticipated and actual PET values may arise due to the limitations in previous reference standards employed for evaluation. A comprehensive evaluation of the accuracy of the PET models in cropland was conducted in this study. Non-water-stressed days with evaporation fraction (EF) exceeding the 95th percentile threshold were selected from cropland sites in the FLUXNET Tier 1 database as the basis for calibrating and validating 22 PET calculation models at the daily scale and 19 at the sub-daily scale. Results indicated that the Penman (1948), Penman (1963), and all radiation-based models showed strong performance, with R2, RMSE, and NSE of 0.82–0.89, 0.58–0.86 mm day−1, and 0.54–0.77 at the daily scale, and 0.73–0.81, 0.04–0.05 mm 0.5 h−1, and 0.62–0.72 at the sub-daily scale, respectively. Four recently developed radiation-based models have exhibited remarkable accuracy on a daily basis. The relative model errors (RMSE/MEAN) tended to decrease as net radiation (Rn), temperature (Ta), and vapor pressure deficit (VPD) increased at both daily and sub-daily time scales. The models displayed strong accuracy in estimating daily PET when Ta > 15 ℃ or 0.4 < VPD < 1.0, with RMSE/MEAN values ranging from 0.10 to 0.22 and R2 values ranging from 0.76 to 0.89. Higher accuracy in sub-daily scale PET estimates was achieved when Ta > 15 ℃ or 0.4 < VPD < 1.6, with RMSE/MEAN of 0.24–0.43 and R2 of 0.54–0.85, respectively. Additionally, the time lag between PET and Rn, Ta, and VPD increased as Rn, Ta, and VPD increased at the sub-daily scale, which may contribute to the reduced precision of sub-daily PET estimates compared to daily scale estimates. These findings suggest that PET models with calibrated parameters have the potential to serve as the basis for estimating cropland AET, and can provide direction for future model improvement.