Selenium is an important element in nutrition, showing great potential in the udder health of dairy cattle and in the control of subclinical mastitis. However, there are few studies on selenium and subclinical mastitis in cows, and the correlation is not clear. A trial was designed to investigate the association between serum selenium levels and the immune and antioxidant capacity of dairy cattle with subclinical mastitis. Fifty cattle in early lactation with similar background information were selected randomly from an intensive dairy farm. Blood samples were collected for the detection of serum selenium levels by ICP-optic emission spectrometer. The cattle were divided into a low-selenium group (< 0.05mg/L) and three normal selenium groups with different serum selenium levels (0.05-0.08mg/L). The somatic cell count, immune indexes, and other indexes in the milk of each group were detected. The somatic cell count was found to be negatively correlated with serum selenium level. GSH-Px had a low positive correlation and IL-6 had a low negative correlation with serum selenium level. With a serum glutathione peroxidase < 148U/L and IL-6 > 451pg/mL, the risk of subclinical mastitis in dairy cattle increased.