Suboptimal Environmental Conditions Research Articles

Range-extending coral reef fishes trade-off growth for maintenance of body condition in cooler waters

As ocean waters warm due to climate change, tropical species are shifting their ranges poleward to remain within their preferred thermal niches. As a result, novel communities are emerging in which tropical species interact with local temperate species, competing for similar resources, such as food and habitat. To understand how range-extending coral reef fish species perform along their leading edges when invading temperate ecosystems, we studied proxies of their fitness, including somatic growth (length increase), feeding rates, and body condition, along a 730-km latitudinal gradient situated in one of the global warming hotspots. We also studied co-occurring temperate species to assess how their fitness is affected along their trailing edges under ocean warming. We predicted that tropical fishes would experience reduced performance as they enter novel communities with suboptimal environmental conditions. Our study shows that although tropical fish maintain their body condition (based on three proxies) and stomach fullness across all invaded temperate latitudes, they exhibit decreased in situ growth rates, activity levels, and feeding rates in their novel temperate environment, likely a result of lower metabolic rates in cooler waters. We posit that tropical fishes face a growth–maintenance trade-off under the initial phases of ocean warming (i.e. at their leading edges), allowing them to maintain their body condition in cooler temperate waters but at the cost of slower growth. Temperate fish exhibited no distinct patterns in body condition and performance along the natural temperature gradient studied. However, in the face of future climate change, when metabolism is no longer stymied by low water temperatures, tropical range-extending species are likely to approach their native-range growth rates along their leading edges, ultimately leading to increased competitive interactions with local species in temperate ecosystems.

Mechanised Rubber Tapping Devices

Kerala is the leading State in India for rubber plantations and grows to 92% of the Country’s total rubber production. Rubber is an important agricultural plantation, rapidly expanding in to both climatically optimal and suboptimal environmental conditions. Tapping of rubber trees is a skill oriented work and the labors availability in this field is a big problem faced today, which affected the natural rubber productivity in the plantations of Kerala. The unskilled labors cannot be used for traditional method of rubber tapping. This condition warrants the need of a semi-automatic or mechanised rubber tapping machine, which doesn’t need labor skill. The present study explains the Designs and other specifications of four Models of mechanised Rubber Tapping Devices

Effect of Preharvest Abiotic Stresses on the Accumulation of Bioactive Compounds in Horticultural Produce.

The quality of horticultural products is the result of the interaction of different factors, including grower’s crop management ability, genotype, and environment. Sub-optimal environmental conditions during plant growth can induce abiotic stresses and reduce the crop performance with yield reduction and quality losses. However, abiotic stresses can induce several physiological, biochemical, and molecular responses in plants, aiming to cope with the stressful conditions. It is well known that these abiotic stresses are also elicitors of the biosynthesis of many metabolites in plants, including a wide range of bioactive compounds, which firstly serve as functional molecules for crop adaptation, but they have also a great interest for their beneficial effects on human health. Nowadays, the consumer is oriented to low-energy foods with low fat content, but at the same time, growing attention is paid to the presence of bioactive molecules, which are recognized as health-related compounds and concur to the nutraceutical value of plant-derived foods. In this context, fruit and vegetables play an important role as sources of bioactive ingredients in the diet. At the cultivation level, the understanding of crop responses to abiotic stresses and how they act in the biosynthesis/accumulation of these bioactive compounds is crucial. In fact, controlled abiotic stresses can be used as tools for improving the nutraceutical value of fruit and vegetables. This review focuses on the quality of vegetables and fruits as affected by preharvest abiotic stressors, with particular attention to the effect on the nutraceutical aspects.

Evaluating the accuracy of satellite-derived evapotranspiration estimates acquired during conditions of water stress

ABSTRACT Surface energy balance models such as the surface energy balance system (SEBS) assume that the influence of soil moisture and biophysical parameters on evapotranspiration (ET), are implicitly encompassed in model input variables such as the land surface temperature. This simplification may generally be suitable for environments in which the available energy constrains ET but may prove to be inadequate during suboptimal environmental conditions. To address this limitation, the SEBSESF approach was proposed to better represent the influence of environmental stress on surface energy fluxes, using an empirically derived calibration factor to adjust the evaporative fraction previously determined in SEBS. SEBSESF was evaluated by comparing the modelled ET estimates against Eddy Covariance ET measurements (ECET) at two sites, with contrasting environmental conditions. Comparisons between ECET and modelled ET indicated that the implementation of SEBSESF resulted in an improved correlation between ECET and modelled ET estimates, as well as an increase in the percentage of modelled ET estimates within an acceptable accuracy range (± 15 to 30%). Overall, the results of the study demonstrate how potential improvements in the mapping of ET within arid and semi-arid environments can be achieved by accounting for environmental stress. This in turn provides an opportunity to better understand and quantify energy and water fluxes, using satellite-based ET estimation approaches.

Human Blastocyst Biopsy and Vitrification.

Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the diagnostic techniques and to transfer the selected embryo(s) on a physiological endometrium in a following natural cycle. A biopsy approach encompassing the sequential opening of the zona pellucida and retrieval of 5-10 trophectoderm cells (ideally 7-8) limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions. After proper training, the technique was reproducible across different operators in terms of timing of biopsy (~8 min, ranging 3-22 min based on the number of embryos to biopsy per dish), conclusive diagnoses obtained (~97.5%) and live birth rates after vitrified-warmed euploid blastocyst transfer (>40%). The survival rate after biopsy, vitrification and warming was as high as 99.8%. The re-expansion rate at 1.5 h from warming was as high as 97%, largely dependent on the timing between biopsy and vitrification (ideally ≤30 min), blastocyst morphological quality and day of biopsy. In general, it is better to vitrify a collapsed blastocyst; therefore, in non-PGT cycles, laser-assisted artificial shrinkage might be performed to induce embryo collapse prior to cryopreservation. The most promising future perspective is the non-invasive analysis of the IVF culture media after blastocyst culture as a putative source of embryonic DNA. However, this potential avant-garde is still under investigation and a reliable protocol yet needs to be defined and validated.

The combined effects of salinity, hypoxia, and oil exposure on survival and gene expression in developing sheepshead minnows, Cyprinodon variegatus

The 2010 Deepwater Horizon oil spill released approximately 780 million liters of crude oil contaminating coastal habitats from Texas to Florida which are important habitats for many fish species during early life stages. These diverse habitats are also prone to rapid fluctuations in water quality, such as dissolved oxygen concentration and salinity. The consequence of combined exposure to crude oil and suboptimal environmental conditions during early life stage development of fish is still largely unknown. The objective of this project was to investigate the impacts of exposure to crude oil in combination with varying environmental stressors on developing Cyprinodon variegatus survival, growth, and gene expression. Three life stages (embryonic, post-hatch, and post-larval) were exposed to four nominal concentrations (6.25%, 12.5%, 50% and 100% with actual polycyclic aromatic hydrocarbon (PAH) concentrations ranging from 0 to 512 μg/L) of high energy water accommodated fractions (HEWAF) under different oxic (2.0 or >5.0 mg/L) and salinity (10 or 30 ppt) regimes at 30 °C for 48 h. We found that the post-larval developmental stage was the most sensitive to oil toxicity. Median lethal concentrations during the post-larval exposures followed a treatment-dependent pattern with the highest mortality observed under hypoxic-high salinity conditions (64.55 μg/L). Real-time PCR analysis identified down regulation of target genes, encoding cytochrome P450-1α (cyp1a1), erythropoietin (epo), and the aryl hydrocarbon receptor nuclear translocator (arnt1) only when oil exposure occurred under hypoxic-high salinity conditions in treatments with PAH concentrations greater than 226 μg/L. The target genes measured in this experiment are involved in the aryl hydrocarbon receptor signaling pathway which modulates metabolism of PAHs (a major component of crude oil), and the hypoxia inducible 1-α signaling pathway which is responsible for resilience to hypoxic stress, and it is known that disruption of these pathways can lead to an array of acute and chronic effects. Our results indicated that sheepshead minnow are most sensitive to oil exposure during the post-larval developmental stage. Survival data from this age-stage also indicate that oil toxicity response is exacerbated in hypoxic and high salinity environments. The increased mortality observed during the post-larval developmental stage might be attributed to the suppression of the aryl hydrocarbon receptor signaling and the hypoxia inducible 1-α signaling pathways which is evident in by the down-regulated expression of cyp1a1, epo, and arnt1. These findings provide more information about interactions between oil and abiotic factors which enable us to make better assumptions of the ecological impacts of DWH on coastal estuaries.

Combined effects of salinity, temperature, hypoxia, and Deepwater Horizon oil on Fundulus grandis larvae

Oil spills have polluted the marine environment for decades and continue to be a major source of polycyclic aromatic hydrocarbons (PAHs) to marine ecosystems around the globe, for example during the 2010 Deepwater Horizon spill. Although the toxicity of PAHs to fish has been well studied, their effects combined with abiotic stressors are poorly understood. The goal of this study was to describe the combined impacts of crude oil and environmental stressors on fish larvae, a sensitive life stage. Gulf killifish (Fundulus grandis) larvae (<24 h post-hatch) were exposed for 48 h to high energy water accommodated fractions (HEWAF; total PAHs 0–125 ppb) of Macondo oil from the Deepwater Horizon spill under different combinations of environmental conditions (dissolved oxygen 2, 6 ppm; temperature 20, 25, 30 °C; salinity 3, 10, 30 ppt). Even under optimal environmental conditions (25 °C, 10 ppt, 6 ppm) larval survival and development were negatively affected by PAHs, starting with the lowest concentration tested (∼15 ppb). Hypoxia and high temperature each increased the adverse effects of HEWAF on development and mortality. In contrast, salinity had little effect on any of the endpoints measured. Importantly, expression of the detoxifying gene cyp1a was highly induced in PAH-exposed larvae under normoxic conditions, but not under hypoxic conditions, potentially explaining the enhanced toxicity observed under hypoxia. This work highlights the importance of considering how suboptimal environmental conditions can exacerbate the effects of pollution on fish early life stages.

Prolactin and human weight disturbances: A puzzling and neglected association.

Weight gain at the outset of prolactinomas in many women is well documented. Yet, this symptom is absent from the clinical descriptions of the disease in textbooks and reviews. This omission is almost certainly due to the absence of a physiological explanation for the phenomenon, as prolactin is not a recognized fat promoting hormone. In this review we present the clinical evidence for a relationship between prolactin and fat accumulation and address some possible mechanisms involved. We put forward the hypothesis that prolactin is a component of a neuroendocrine program - maternal subroutine - aimed at optimizing the care of the young through the production of milk, promotion of maternal behavior and increase in the metabolic efficiency of the mother. These adaptations can enable her to face the extraordinary metabolic expenses of pregnancy and nursing, especially during times of suboptimal environmental conditions. We emphasize the uniqueness of prolactin in that it is a hormone that is tonically inhibited and which has its major effects on the regulation of an inter-individual (the mother - offspring dyad), rather than an intra-individual, system. This approach opens a window to consider the possibility of external events as regulators of this system. It also allows addressing a variety of hitherto unexplained findings reported in the literature. Examples include: association of prolactinomas with paternal deprivation and with stressful life events; pseudocyesis; acute life event-driven episodes of galactorrhea; episodes of rapid weight gain following a life event; prolactin surges (without associated cortisol surges) following some psychological stresses.

Defining features of age-specific fertility and seed quality in senescing indeterminate annuals.

A trade-off between fertility and offspring viability underpins plantreproductive response to sub-optimal environmental conditions. Senescence involves internal resource limitation, and it is a sub-optimal body condition. We tested if senescence affects age-specific fertility and seed viability (quality) in indeterminate annuals. Fertility in individual pods on the monopodial indeterminate inflorescence of Arabidopsis thaliana and its big-seeded relative Brassica nigra was quantified. The reproductive phase was divided into three phases: (1) early-senescence (initial flowers) (2) mid-senescence and (3) late-senescence (wilting leaves). Seed-viability probability as a function of pod position on the inflorescence (a proxy for parent's age) and seed position within pod was verified by germination tests in Brassica and then analysed using a binomial logistic regression model. Age-specific fertility increased gradually, peaked, and then declined significantly during senescence in Arabidopsis and Brassica. Acropetal size distribution of rosette leaves was similar to that of pods (age-specific fertility) in Arabidopsis. Seeds positioned closest to stigma tended to be heavier and more viable than others in highly fertile pods, characteristic of mid-senescence phase in Brassica. Pod position (parent's age) was a significant predictor of seed-viability probability or seed quality, which improved in old and senescing Brassica. High viability probability of seeds produced in low-fertility pods during late-senescence phase suggests weakening of maternal control over seed-size optimization (bigger, fewer, and better seeds) in internally resource-depleted older parent plants. Proximity to stigma can increase seed quality. The unexpected increase in fertility and seed viability during early-senescence phase is likely due to highly conserved developmental constraints on leaf and pod phenotype. Indeterminate annuals can shed light on fertility, offspring quality and senescence relationships in all plants that reproduce sexually and indeterminately.

Ecophysiolomic analysis of stress tolerant Himalayan shrub Hipppophae rhamnoides shows multifactorial acclimation strategies induced by diverse environmental conditions.

Climatic fluctuations are a major global concern, affecting the agronomic productivity of plants. Hippophae rhamnoides a naturally growing stress tolerant Himalayan shrub was chosen to understand its stress hardiness mechanism. Comparative proteomic and biochemical analysis were done for pooled berry populations (HrB13 and HrB14) growing in two different environmental conditions. HrB13, growing under sub-optimal environmental conditions exhibited differential abundance of stress responsive proteins, which were the rate limiting enzymes associated with stress-responsive metabolic pathways, including Xanthine dehydrogenase (reactive oxygen species [ROS] signaling), Farnesyl diphosphate synthase (phenylpropanoid pathway), endosomal BRO-1 domain protein (ultraviolet [UV]-light stress), Phosphofructokinase (sugar metabolism) and Ubiquitin thioesterase (protein alterations). Biochemical investigations showed a positive correlation between proteomic plasticity (HrB13) and 1.6 to 15-fold accumulation of downstream adaptive metabolic signatures like enzymes and antioxidants involved in ROS scavenging pathways (Catalase, Ascorbate peroxidase, Glutathione reductase, ascorbate and glutathione content), secondary metabolites (phenolics, flavonoids, carotenoids) and polyunsaturated fatty acids (∝ - linolenic acid and linoleic acid). Interactome and KEGG pathway analysis also supported interactions of differentially accumulated proteins with stress-responsive signaling components involved in physiological pathways associated with stress tolerance. This is the first 'ecophysiolomics' study, showing the response of seabuckthorn to multiple stress conditions via activation of multifactorial acclimation strategies leading to morphological, metabolic and physiological modifications, resulting in dark orange berries in HrB13. Higher accumulation of omega-6 fatty acids, carotenoids and ascorbate during suboptimal growth conditions, provides exciting prospects for enhancing pharmaceutical properties of seabuckthorn berries, emphasizing need to analyze diversity of plant signaling mechanisms under changing climate conditions.

Cool-season Grasses for Overseeding Sport Turfs: Germination and Performance under Limiting Environmental Conditions

In the transition zone, warm-season grasses are often overseeded with diploid perennial ryegrass (Lolium perenne L., 2n = 2x = 14) to provide a temporary green surface for winter sporting activities. Because improved cultivars of perennial ryegrass will often persist into summer in overseeded turf, alternative cool-season grasses have been developed to facilitate more rapid transition back to the warm-season species. Limited information is available on these alternative species, especially with regard to their germination characteristics under shade and performance under limiting factors, such as low temperature and restricted photoperiod. Greenhouse and growth chamber studies were designed to test four alternative overseeding grasses in comparison with diploid perennial ryegrass, to verify their potential use in the artificial environment of modern stadiums. Meadow fescue (Festuca pratensis Huds.), tetraploid perennial ryegrass (Lolium perenne L., 2n = 4x = 28), annual ryegrass (Lolium multiflorum Lam.), and spreading diploid perennial ryegrass [Lolium perenne L. subsp. stoloniferum (C. Lawson) Wipff.] were tested. Six different shade treatments were used in the greenhouse study, including 30%, 50%, 70%, 90%, and 100% shade and a nonshaded control (0% shade). Germination was monitored daily over a 21-day period by counting and removing emerged seedlings. The experimental design for this study was a randomized complete block design, with four replications of each species and shade level for a total of 120 experimental units. In the growth chamber study, the same plant material was tested simulating optimal, suboptimal, and critical environmental conditions that can be potentially found within a modern sport facility. In the greenhouse study, the highest final germination was observed with annual ryegrass at 90% shade (98.7%), whereas the lowest for tetraploid perennial ryegrass at 30% shade (58.8%). Annual ryegrass was the fastest emerging species, whereas meadow fescue the slowest. In the growth chamber study, in comparison with perennial ryegrass, the following results may be summarized: 1) meadow fescue and tetraploid ryegrass showed coarser leaf texture, similar growth rates and Normalized Difference Vegetation Index (NDVI) value; 2) annual ryegrass had similar leaf texture, accelerated growth characteristics, and lower NDVI value; and 3) spreading perennial ryegrass displayed finer leaf texture, lower vertical growth, and similar NDVI value.

МОЛЕКУЛЯРНЫЕ МЕХАНИЗМЫ ТОЛЕРАНТНОСТИ РАСТЕНИЙ К ВИРУСНЫМ ПАТОГЕНАМ: ИЗМЕНЕНИЯ ТРАНСКРИПЦИОННЫХ ПРОГРАММ ЭКСПРЕССИИ ФАКТОРОВ АУТОФАГИИ И RQC (RNA QUALITY CONTROL) В ПРОЦЕССЕ ПЕРСИСТЕНТНОЙ АЛЛЕКСИВИРУСНОЙ ИНФЕКЦИИ

The two major mechanisms of plant defense against pathogens including viruses are resistance (the host’s ability to limit pathogen multiplication) and tolerance (the host’s ability to reduce the effect of infection on its fitness regardless of the level of pathogen multiplication) [1]. Tolerance is evolutionary more stable defense response and is effected against a larger spectrum of virus isolates compared to resistance; the selection pressure for emergence of virulent strains is also reduced in tolerant cultivars compared to resistant one, and persistent virus infection can improve the plant resilience in sub-optimal environmental conditions [2]. On the basis of our preliminary results [3, 4] we hypothesized that the plant tolerant reaction against virus infection may be in particular the consequence of transcriptomic reprogramming of autophagy and RNA-decay, the key processes of innate plant immunity. So, the task of this study is the experimental verification of this hypothesis by quantitative real-time PCR ascertainment of the autophagy and ROC (RNA quantity control) factors expression changes during persistent shallot virus X infection [5]. The research has been conducted at Molecular Virology Laboratory and Center of collective sharing of the All-Russian Research Institute of Agricultural Biotechnology. In this context the autophagy and RNA-decay factors coding transcripts have been identified and their representation levels were determined in shallot plants. It was drawn a conclusion that persistent Shallot virus infection correlates with the specific autophagy and ROC factors expression changes. Further research on the molecular mechanisms associated with tolerance may identify novel targets for engineering tolerance to improve agriculture practices.

Compliant plant wearables for localized microclimate and plant growth monitoring

The microclimate surrounding a plant has major effect on its health and photosynthesis process, where certain plants struggle in suboptimal environmental conditions and unbalanced levels of humidity and temperature. The ability to remotely track and correlate the effect of local environmental conditions on the healthy growth of plants can have great impact for increasing survival rate of plants and augmenting agriculture output. This necessitates the widespread distribution of lightweight sensory devices on the surface of each plant. Using flexible and biocompatible materials coupled with a smart compact design for a low power and lightweight system, we develop widely deployed, autonomous, and compliant wearables for plants. The demonstrated wearables integrate temperature, humidity and strain sensors, and can be intimately deployed on the soft surface of any plant to remotely and continuously evaluate optimal growth settings. This is enabled through simultaneous detection of environmental conditions while quantitatively tracking the growth rate (viz. elongation). Finally, we establish a nature-inspired origami-assembled 3D-printed “PlantCopter”, used as a launching platform for our plant wearable to enable widespread microclimate monitoring in large fields.

Adrenocortical and bioenergetic responses to cold in laboratory-born northern red-backed voles (Myodes rutilus) from two populations in south Siberia, Russia

In animal populations inhabiting ecologically suboptimal environmental conditions, phenotypical shifts in physiological traits responsible for coping with environmental challenges can be expected. If such variations are of heritable origin, then they will manifest themselves even in individuals bred in captivity. In laboratory-born red-backed voles (Myodes rutilus (Pallas, 1779)) originating from a population with constantly low density, maximum cold-induced metabolic rates were higher than in voles from a high-density population, similar to the data obtained on wild-caught individuals from the same populations. However, unlike wild-caught voles, in laboratory-born individuals maintained under comfortable conditions, we revealed no interpopulation differences either in basal plasma corticosterone level or in corticosterone response to acute cooling. These data confirm the suggestion about the heritable origin of increased maximum cold-induced metabolic rate in a red-backed vole population with relatively low density.

Condition and reproductive strategy of the Argentina sphyraena, a cold-water species in the Mediterranean Sea

We evaluate the condition and reproductive status of the lesser silver smelt, Argentina sphyraena in the north-western Mediterranean Sea. We describe for the first time several life history traits of this species, i.e. condition, reproductive cycle, fecundity, breeding strategy, oocyte recruitment and egg quality, and evaluate the links between these traits. We also contrast condition and reproduction parameters in two study areas with different environmental conditions: the Gulf of Lion and the Balearic Sea. Our results indicate that A. sphyraena is a capital breeder with group-synchronous oocyte development and determinate fecundity. Energy requirements for reproduction are mostly met by lipids stored in the mesentery which is the main fat deposit and a good indicator of condition; this means that condition can be estimated visually and easily using a fat scaling technique. Our results show that, in terms of condition and reproduction, this species performs better in the colder and more productive Gulf of Lion than in the warmer and less productive Balearic Sea, as evidenced by the amount of stored energy and reproductive strategy. These findings suggest that suboptimal environmental conditions may have a negative impact on the condition and reproduction strategy of cold-water species, which will ultimately affect their abundance and distribution.

Prothoracicotropic hormone modulates environmental adaptive plasticity through the control of developmental timing.

Adult size and fitness are controlled by a combination of genetics and environmental cues. In Drosophila, growth is confined to the larval phase and final body size is impacted by the duration of this phase, which is under neuroendocrine control. The neuropeptide prothoracicotropic hormone (PTTH) has been proposed to play a central role in controlling the length of the larval phase through regulation of ecdysone production, a steroid hormone that initiates larval molting and metamorphosis. Here, we test this by examining the consequences of null mutations in the Ptth gene for Drosophila development. Loss of Ptth causes several developmental defects, including a delay in developmental timing, increase in critical weight, loss of coordination between body and imaginal disc growth, and reduced adult survival in suboptimal environmental conditions such as nutritional deprivation or high population density. These defects are caused by a decrease in ecdysone production associated with altered transcription of ecdysone biosynthetic genes. Therefore, the PTTH signal contributes to coordination between environmental cues and the developmental program to ensure individual fitness and survival.

Interplay between the unfolded protein response and reactive oxygen species: a dynamic duo.

Secretory proteins undergo modifications such as glycosylation and disulphide bond formation before proper folding, and move to their final destination via the endomembrane system. Accumulation of unfolded proteins in the endoplasmic reticulum (ER) due to suboptimal environmental conditions triggers a response called the unfolded protein response (UPR), which induces a set of genes that elevate protein folding capacity in the ER. This review aims to establish a connection among ER stress, UPR, and reactive oxygen species (ROS), which remains an unexplored topic in plants. For this, we focused on mechanisms of ROS production originating from ER stress, the interaction between ER stress and overall ROS signalling process in the cell, and the interaction of ER stress with other organellar ROS signalling pathways such as of the mitochondria and chloroplasts. The roles of the UPR during plant hormone signalling and abiotic and biotic stress responses are also discussed in connection with redox and ROS signalling.

Nonadditive effects of consumption in an intertidal macroinvertebrate community are independent of food availability but driven by complementarity effects.

Suboptimal environmental conditions are ubiquitous in nature and commonly drive the outcome of biological interactions in community processes. Despite the importance of biological interactions for community processes, knowledge on how species interactions are affected by a limiting resource, for example, low food availability, remains limited. Here, we tested whether variation in food supply causes nonadditive consumption patterns, using the macroinvertebrate community of intertidal sandy beaches as a model system. We quantified isotopically labeled diatom consumption by three macroinvertebrate species (Bathyporeia pilosa, Haustorius arenarius, and Scolelepis squamata) kept in mesocosms in either monoculture or a three‐species community at a range of diatom densities. Our results show that B. pilosa was the most successful competitor in terms of consumption at both high and low diatom density, while H. arenarius and especially S. squamata consumed less in a community than in their respective monocultures. Nonadditive effects on consumption in this macroinvertebrate community were present and larger than mere additive effects, and similar across diatom densities. The underlying species interactions, however, did change with diatom density. Complementarity effects related to niche‐partitioning were the main driver of the net diversity effect on consumption, with a slightly increasing contribution of selection effects related to competition with decreasing diatom density. For the first time, we showed that nonadditive effects of consumption are independent of food availability in a macroinvertebrate community. This suggests that, in communities with functionally different, and thus complementary, species, nonadditive effects can arise even when food availability is low. Hence, at a range of environmental conditions, species interactions hold important potential to alter ecosystem functioning.

The Future of Coral Reefs Subject to Rapid Climate Change: Lessons from Natural Extreme Environments

Global climate change and localised anthropogenic stressors are driving rapid declines in coral reef health. In vitro experiments have been fundamental in providing insight into how reef organisms will potentially respond to future climates. However, such experiments are inevitably limited in their ability to reproduce the complex interactions that govern reef systems. Studies examining coral communities that already persist under naturally-occurring extreme and marginal physicochemical conditions have therefore become increasingly popular to advance ecosystem scale predictions of future reef form and function, although no single site provides a perfect analogue to future reefs. Here we review the current state of knowledge that exists on the distribution of corals in marginal and extreme environments, and geographic sites at the latitudinal extremes of reef growth, as well as a variety of shallow reef systems and reef-neighbouring environments (including upwelling and CO2 vent sites). We also conduct a synthesis of the abiotic data that have been collected at these systems, to provide the first collective assessment on the range of extreme conditions under which corals currently persist. We use the review and data synthesis to increase our understanding of the biological and ecological mechanisms that facilitate survival and success under sub-optimal physicochemical conditions. This comprehensive assessment can begin to: (i) highlight the extent of extreme abiotic scenarios under which corals can persist, (ii) explore whether there are commonalities in coral taxa able to persist in such extremes, (iii) provide evidence for key mechanisms required to support survival and/or persistence under sub-optimal environmental conditions, and iv) evaluate the potential of current sub-optimal coral environments to act as potential refugia under changing environmental conditions. Such a collective approach is critical to better understand the future survival of corals in our changing environment. We finally outline priority areas for future research on extreme and marginal coral environments, and discuss the additional management options they may provide for corals through refuge or by providing genetic stocks of stress tolerant corals to support proactive management strategies.

Spatiotemporal genetic structure of anadromous Arctic char (Salvelinus alpinus) populations in a region experiencing pronounced climate change

We examined spatio-temporal genetic variation at 53 single nucleotide polymorphisms in anadromous Arctic char populations from Western Greenland, a region experiencing pronounced climate change. The study was based on contemporary and historical samples, the latter represented by DNA extracted from otoliths and scales from the 1950s–1960s. We investigated whether genetic population structure was temporarily stable or unstable, the latter due to relatively small spawning and nursery areas combined with a harsh Arctic environment. Furthermore, in order to evaluate the potential for adaptive responses and local adaptation we estimated effective population size (Ne) and migration rate (m). Temporal stability of genetic population structure was suggested, based on a hierarchical analysis of genetic differentiation showing much higher differentiation among samples from different populations (FCT = 0.091) than among temporal samples from the same populations (FSC = 0.01). This was further supported by a neighbor-joining tree and assignment of individuals that showed high contingency between historical and contemporary samples. Estimates of Ne were high (> 500) in three out of four populations, with a lower estimate in one population potentially reflecting fishing pressure or suboptimal environmental conditions. Estimates of m were in most cases low, ≤ 0.01. Ne and m estimates suggest a potential for adaptive responses and local adaptation. However, long generation time may also cause adaptive responses by microevolution to be unable to track climate change, especially considering the low migration rates that reduce potential evolutionary rescue by gene flow from populations better adapted to the altered environments.