МОЛЕКУЛЯРНЫЕ МЕХАНИЗМЫ ТОЛЕРАНТНОСТИ РАСТЕНИЙ К ВИРУСНЫМ ПАТОГЕНАМ: ИЗМЕНЕНИЯ ТРАНСКРИПЦИОННЫХ ПРОГРАММ ЭКСПРЕССИИ ФАКТОРОВ АУТОФАГИИ И RQC (RNA QUALITY CONTROL) В ПРОЦЕССЕ ПЕРСИСТЕНТНОЙ АЛЛЕКСИВИРУСНОЙ ИНФЕКЦИИ

Abstract

The two major mechanisms of plant defense against pathogens including viruses are resistance (the host’s ability to limit pathogen multiplication) and tolerance (the host’s ability to reduce the effect of infection on its fitness regardless of the level of pathogen multiplication) [1]. Tolerance is evolutionary more stable defense response and is effected against a larger spectrum of virus isolates compared to resistance; the selection pressure for emergence of virulent strains is also reduced in tolerant cultivars compared to resistant one, and persistent virus infection can improve the plant resilience in sub-optimal environmental conditions [2]. On the basis of our preliminary results [3, 4] we hypothesized that the plant tolerant reaction against virus infection may be in particular the consequence of transcriptomic reprogramming of autophagy and RNA-decay, the key processes of innate plant immunity. So, the task of this study is the experimental verification of this hypothesis by quantitative real-time PCR ascertainment of the autophagy and ROC (RNA quantity control) factors expression changes during persistent shallot virus X infection [5]. The research has been conducted at Molecular Virology Laboratory and Center of collective sharing of the All-Russian Research Institute of Agricultural Biotechnology. In this context the autophagy and RNA-decay factors coding transcripts have been identified and their representation levels were determined in shallot plants. It was drawn a conclusion that persistent Shallot virus infection correlates with the specific autophagy and ROC factors expression changes. Further research on the molecular mechanisms associated with tolerance may identify novel targets for engineering tolerance to improve agriculture practices.