Chlorpyrifos, a widely used organophosphate pesticide, poses significant environmental and health risks due to its prolonged persistence and toxicity. Hence the biodegradation of chlorpyrifos has immense significance for its effective removal, and the present study has been designed on the management of chlorpyrifos pollution through an innovative bioremediation approach. Here, Pseudomonas sp. CF7b isolated from the soil has been investigated for chlorpyrifos removal in the presence of sub-inhibitory concentrations (sub-MIC) of zinc oxide nanoparticles (ZnONPs). For this, chlorpyrifos-tolerant bacteria were initially isolated from the soil and out of the 29 bacterial isolates obtained, Pseudomonas sp. CF7b was selected for the detailed study. The selected organism was then treated with chlorpyrifos in the presence of sub-inhibitory concentrations of ZnONPs. The quantitative reduction of chlorpyrifos under the experimental conditions was further analyzed using gas chromatography-mass spectrometry (GC-MS). Here, chlorpyrifos treated in the presence of both Pseudomonas sp. CF7b and sub-MIC ZnONPs was found to undergo enhanced reduction when compared with the quantitative changes observed for the treatment containing the organism or ZnONPs alone. After 120 hof incubation, a 90.9 % reduction was observed in chlorpyrifos for the treatment containing both Pseudomonas sp. CF7b and ZnONPs. In comparison, the reduction was 81.6 % for samples treated with Pseudomonas sp. CF7b alone and 53.4 % for samples treated with ZnONPs alone. The observation from the current study provides valuable insights into the role of nanomaterials in enhancing the microbial action against environmental pollutants and hence opens up the scope for the development of novel sustainable methods for the environmental management of pesticides.