Traditional teaching methods alone are no longer sufficient to meet the needs of modern societies. This study aimed to compare the effectiveness of central venous pressure teaching through multimedia and lecture method on learning rate of anesthesia technologist students. In this quasi-experimental study, 41 undergraduate students of anesthesia technologist were selected through the census method and randomly divided into two groups: multimedia- and lecture-based teaching. The educational content was the same for both groups and was taught by the same educator. A pre-test was administered to both groups, and a post-test was conducted two weeks after the intervention. The data were analyzed using descriptive statistics, independent t-test, and paired t-test with SPSS software version 26. Before the intervention, there was no significant difference in the mean scores between the two groups (P = 0.648). However, after the intervention, the learning rate significantly improved in both teaching methods (P < 0.001). Furthermore, the mean scores obtained from post-test increase in the multimedia-based group were significantly higher than the lecture-based group (P = 0.004). The results of this study indicate the effectiveness of both methods. However, considering the advantages of multimedia-based teaching, such as responding to various learning styles and higher mean scores obtained from post-test of the multimedia-based group compared to the lecture-based group, it is recommended to utilize this teaching method in medical science courses.