The traditional corn grain harvester threshing device has the problems of high grain breakage rate, high unthreshed grain rate and high energy consumption when harvesting high water content corn ears. A longitudinal-flow corn ear threshing device and its measurement and control system were proposed to overcome these problems. The working performance and energy consumption of the threshing device were optimized by conducting three factors and five levels corn ear threshing experiments. The experiments took the threshing cylinder speed (X1), threshing gap (X2), and guide vane angle (X3) as the experimental factors, and the grain breakage rate (Y1), unthreshed grain rate (Y2), unit energy consumption (Y3), and threshing concave pressure (Y4) as the experimental indicators. According to the experimental results, the quadratic polynomial regression models between experimental indicators and experimental factors were established. Through models optimization, the optimal working parameters for the threshing device were obtained as X1 at 392.40 r/min, X2 at 40.70 mm, and X3 at 33.16°. Through conducting verification experiment, it was found that under the optimal parameters, the experimental indicators were Y1 at 2.32 ± 0.06 %, Y2 at 0.48 ± 0.04 %, Y3 at 2.14 ± 0.10 kJ/kg, and Y4 at 46.28 ± 3.23N. The errors between the experimental results and the quadratic polynomial regression models were less than 5 %, which indicated that the models had high prediction accuracy. Compared with the unoptimized experimental indicators, the optimized experimental indicators Y1, Y2, Y3 and Y4 decreased by 36.26 %, 39.24 %, 14.74 % and 16.27 %, respectively. The working performance of the threshing device was significantly improved, and the energy consumption was significantly reduced. The relevant research could provide reference for the optimization of the mechanical structure and working parameters of the corn ear threshing device.