Structural Functional Properties Research Articles

Light-Induced Transient Lattice Dynamics and Metastable Phase Transition in CH3NH3PbI3 Nanocrystals.

Methylammonium lead iodide (MAPbI3) perovskite nanocrystals (NCs) offer desirable optoelectronic properties with prospective utility in photovoltaics, lasers, and light-emitting diodes (LEDs). Structural rearrangements of MAPbI3 in response to photoexcitation, such as lattice distortions and phase transitions, are of particular interest, as these engender long carrier lifetime and bolster carrier diffusion. Here, we use variable temperature X-ray diffraction (XRD) and synchrotron-based transient X-ray diffraction (TRXRD) to investigate lattice response following ultrafast optical excitation. MAPbI3 NCs are found to slowly undergo a phase transition from the tetragonal to a pseudocubic phase over the course of 1 ns under 0.02-4.18 mJ/cm2 fluence photoexcitation, with apparent nonthermal lattice distortions attributed to polaron formation. Lattice recovery exceeds time scales expected for both carrier recombination and thermal dissipation, indicating meta-stability likely due to the proximal phase transition, with symmetry-breaking along equatorial and axial directions. These findings are relevant for fundamental understanding and applications of structure-function properties.

Study of complexation of single-stranded poly(rA) and poly(rU) with methylene blue

To reveal the effect of DNA- or RNA-specific low-molecular compounds on cellular processes on the molecular level, we have carried out the studies with the application of spectroscopic methods. It is necessary for the understanding of structural-functional properties of nucleic acids in cell. In this work the interaction of DNA-specific thiazine dye methylene blue (MB) with synthetic polynucleotides poly(rA) and poly(rU) was studied. The interaction of MB with synthetic polyribonucleotides poly(rA) and poly(rU) was examined in the solution with high ionic strength in a wide phosphate-to-dye (P/D) range, using the absorption and fluorescence spectroscopies, as well as the fluorescence 2D spectra and 3D spectra analyses were given. Values of the fluorescence quenching constants for the complexes of MB with poly(rA) and poly(rU) were calculated (KSV is the Stern-Volmer quenching constant). Two different modes of MB binding to single-stranded (ss-) poly(rA) and poly(rU) and to their hybrid double-stranded (ds-) structure – poly(rA)-poly(rU) were identified. This ligand binds to ss-poly(rA) and poly(rA)-poly(rU) by semi-intercalation and electrostatic modes, but to ss-poly(rU) the prevailing mode is the electrostatic interaction. Communicated by Ramaswamy H. Sarma

Gene Expression, Structural Characterization, and Functional Properties of Exopolysaccharide Produced from Potential Probiotic Enterococcus faecalis NOC219 Strain.

This study aimed to reveal the structural characterization and functional properties of microbial EPS-NOC219 material produced by the Enterococcus faecalis NOC219 strain with high EPS yield isolated from yogurt, with simultaneously, demonstrating the potential of this EPS for future industrial applications. According to the results of the analyses made for this aim, it was determined that the NOC219 strain contains the epsB, p-gtf-epsEFG, and p-gtf-P1 genes. In addition, it was also revealed that the EPS-NOC219 structure is expressed by the epsB, p-gtf-epsEFG, and p-gtf-P1 genes and has a heteropolymeric feature consisting of glucose, galactose, and fructose units. According to the results of the analyses made for this aim, it was determined that the EPS-NOC219 structure, which was produced from the NOC219 strain containing the epsB, p-gtf-epsEFG, and p-gtf-P1 genes, had a heteropolymeric structure consisting of glucose, galactose, and fructose units. On the other hand, it was shown that this structure had a thickener property, high heat stability exhibited a pseudoplastic flow behavior, and had a high melting point. This showed that the EPS-NOC219 had high heat stability and could be used as a thickener in heat treatment processes. In addition, it was revealed that it is suitable for plasticized biofilm production. On the other hand, the bioavailability of this structure was demonstrated with its high antioxidant activity (55.84%) against DPPH radicals and high antibiofilm activity against Escherichia coli (77.83%) and Listeria monocytogenes (72.14%) pathogens. These results suggest that the EPS-NOC219 structure may be an alternative natural resource for many industries as it has strong physicochemical properties and a healthy food-grade adjunct.

Ultrasonic assisted extraction of mulberry leaf protein: Kinetic model, structural and functional properties, in vitro digestion

The world's protein resources are in short supply, and to develop a new source of plant protein, this experiment used ultrasonic-assisted technology to extract mulberry leaf proteins, and investigated the effects of different ultrasonic power densities (150, 300, and 450 W/L), ultrasonic temperatures (30, 40, and 50 °C), and ultrasonic time (2.5, 5, 7.5, 10, 12.5, 15, 17.5, and 20 min) on the yield and structure-function properties of mulberry leaf proteins. Peleg’s model was used to describe extraction kinetics. The highest yield of mulberry leaf protein was 30.03 % at 50 °C, 450 W/L, and 20 min, which was 17.6 % higher than that of the control sample. The secondary and tertiary structures of mulberry leaf protein treated by ultrasound were changed, the content of α-helix and β-turn was decreased, the content of random coil and β-sheet was increased, the fluorescence intensity was changed, and the solubility, emulsification, antioxidant capacity, and in vitro digestibility were significantly improved. According to the experimental results, ultrasonic-assisted extraction technology can significantly increase the yield of mulberry leaf protein and effectively improve its structural and functional properties.

Effect of Catechin on Yolk Immunoglobulin Structure and Properties: A Polyphenol-Protein Interaction Approach.

The preparation of the interaction between polyphenols and protein is of great significance for increasing added value and promoting the application of egg yolk immunoglobulin (IgY). This study systematically investigated the effect of catechin on yolk immunoglobulin structural characteristics and functional properties. The binding conditions, force types, molecular conformation, and residual microenvironment of the interaction between catechin and IgY were analyzed by molecular docking technology, UV-vis absorption and fluorescence spectroscopy studies. The results showed that the main binding forces in the complex were hydrogen bonding and van der Waals forces. After the interaction, fluorescence quenching occurred and the maximum emission wavelength was redshifted. The results showed that the microenvironment around IgY increased polarity, increased hydrophilicity and decreased hydrophobicity, and the structure of the peptide chain changed. The bacteriostatic thermal stability of the compound against Escherichia coli and Staphylococcus aureus was lower than that of catechin IgY. The bacteriostatic acid and base stability were higher than that of catechin and IgY. The antioxidant activity was catechin, complex, and IgY, in descending order. The antioxidant activity of catechin and complex was significantly higher than that of IgY. At the same concentration, the apparent viscosity of the three samples was complex, IgY and catechin, in descending order. G' was greater than G" indicating that elastic properties dominate in G". The G' and G" values of the complex were higher than those of the other groups. Rheological results indicated that the complex may have high physical stability. This study provides theoretical support for broadening the application field of IgY and suggest its properties change in the machining process. It also provides new ideas for the development of functional foods from poultry eggs.

Tree visualizations of protein sequence embedding space enable improved functional clustering of diverse protein superfamilies.

Protein language models, trained on millions of biologically observed sequences, generate feature-rich numerical representations of protein sequences. These representations, called sequence embeddings, can infer structure-functional properties, despite protein language models being trained on primary sequence alone. While sequence embeddings have been applied toward tasks such as structure and function prediction, applications toward alignment-free sequence classification have been hindered by the lack of studies to derive, quantify and evaluate relationships between protein sequence embeddings. Here, we develop workflows and visualization methods for the classification of protein families using sequence embedding derived from protein language models. A benchmark of manifold visualization methods reveals that Neighbor Joining (NJ) embedding trees are highly effective in capturing global structure while achieving similar performance in capturing local structure compared with popular dimensionality reduction techniques such as t-SNE and UMAP. The statistical significance of hierarchical clusters on a tree is evaluated by resampling embeddings using a variational autoencoder (VAE). We demonstrate the application of our methods in the classification of two well-studied enzyme superfamilies, phosphatases and protein kinases. Our embedding-based classifications remain consistent with and extend upon previously published sequence alignment-based classifications. We also propose a new hierarchical classification for the S-Adenosyl-L-Methionine (SAM) enzyme superfamily which has been difficult to classify using traditional alignment-based approaches. Beyond applications in sequence classification, our results further suggest NJ trees are a promising general method for visualizing high-dimensional data sets.

Structural characterization and functional properties of egg white protein treated by electron beam irradiation

This study investigated the functional properties and structural characteristics of egg white protein (EWP) powder treated by electron beam irradiation (EBI) at the doses of 5, 10, 20, 30, 50, 70 and 100 kGy. In comparison with the untreated sample (0 kGy), the EBI-induced EWP showed generally better solubility and higher antioxidant capacity by ABTS•+ radical scavenging assay. Furthermore, the emulsifying activity index of EBI-induced EWP increased with increased EBI dose, reaching the maximum value of 15.8 ± 0.4 m2/g at 100 kGy. The emulsifying stability index and ϛ-potential of EBI-induced EWP showed fluctuating tendencies. These functional property changes of protein were attributed to the conformational changes caused by EBI. SDS-PAGE patterns confirmed that high-dose EBI would result in protein depolymerization to low-mass molecules at 11 kDa, contributing to the increased solubility. Circular dichroism data demonstrated that EBI-induced EWP exhibited partial denaturation and a flexible structure, which was responsible for the improved emulsifying properties. Fluorescence spectra revealed that high EBI doses led to an increase in the polar environment around tryptophan, which could enhance the solubility and antioxidant activity of EWP. In addition, high EBI doses decreased the content of free and total sulphydryl groups, and changed the intermolecular forces of protein, including ionic bonds, hydrogen bonds, hydrophobic interactions, and disulfide bonds. These findings can provide solid support for practical applications of EBI technology in EWP food processing.

Re-entrant relaxor ferroelectric behaviour in Nb-doped BiFeO3–BaTiO3 ceramics

Re-entrant relaxor ferroelectric behaviour is identified in BiFeO3–BaTiO3 ceramics, yielding anomalous thermal variations in structural distortion and functional properties.

РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЙ ДЕНТАЛЬНЫХ ИМПЛАНТАТОВ, УСТАНОВЛЕННЫХ ПАЦИЕНТАМ ПОСЛЕ ПРОВЕДЕНИЯ КОСТНО-ПЛАСТИЧЕСКИХ ОПЕРАЦИЙ ПО УВЕЛИЧЕНИЮ РЕДУЦИРОВАННЫХ АЛЬВЕОЛЯРНЫХ ОТРОСТКОВ ЧЕЛЮСТЕЙ МЕТОДОМ ЧАСТОТНО-РЕЗОНАНСНОГО АНАЛИЗА

The use of dental implants being the intraosseous artificial fixtures is often required for orthopedic rehabilitation of completely edentulous patients; for their placement, adequate amount of bone tissue is needed, which in many cases is insufficient due to physiological bone loss, long-term wearing of full dentures, the duration of edentulism and complex clinical situations caused by maxillofacial injuries or general somatic diseases. Implant placement in the above situations requires timely preparation of deficient alveolar ridge by means of osteoplastic surgery. In its turn, the newly formed regenerated bone by its histological, physicomechanical and structural-functional properties can only approach the recipient bone tissue; therefore, the issue of implant osseointegration and its functional loading in regenerated bone remains debatable. This research uses the method of frequency-resonance analysis showing implant stability coefficient numerically equal to the ISQ (Implant stability Quotient) values and reflecting the percentage of the osseointegration area of the intraosseous part of the implant. The results of research obtained by means of frequency-resonance analysis have proved that with the timely start of implant usage and adequate functional loading, ISQ values increase, that is, increases the osseointegration area along the interface of the intraosseous part of the implant placed in regenerated bone. Comparative results of research reflecting ISQ values in 12 months after implant placement in bone augmented using various forms of autogenous bonegrafts, have been presented; therefore, a conclusion can be made that there is a direct relationship between osseointegration under conditions of functional chewing load and the shape and size of autogenous bonegrafts.

Effects of dielectric barrier discharge plasma treatment on the structural orders and functional properties of chickpea starch

SummaryDielectric barrier discharge (DBD) plasma with different generation currents was introduced to modify chickpea starches of Kabuli and Desi varieties. The changes of starch structural orders and functional properties were investigated. Results demonstrated that the crystalline structure of starches was generally destroyed upon plasma treatment, while the change of double‐helical order was relatively limited. X‐ray photoelectron spectroscopy revealed that DBD plasma altered the surface molecular structure of starch, mainly involving oxidation effects. The gelatinization transition temperatures of plasma‐treated Kabuli starch (KS) mainly remained unchanged, while those of Desi starch (DS) significantly decreased. DBD plasma dramatically reduced the pasting viscosity of KS with the increase of treatment intensity, as well as the amylose content and swelling power, while had little influence on DS. Furthermore, the digestion rate and extent of chickpea starches gradually increased with the treatment intensity. Correlation analysis revealed that the starch digestibility induced by DBD plasma was largely depending on the crystalline structure, while the double helical order had little influence. These findings could provide theoretical basis for exploiting DBD plasma as a new strategy to modify chickpea starch.

Potential implications of oxidative modification on dietary protein nutritional value: A review

During food processing and storage, proteins are sensitive to oxidative modification, changing the structural characteristics and functional properties. Recently, the impact of dietary protein oxidation on body health has drawn increasing attention. However, few reviews summarized and highlighted the impact of oxidative modification on the nutritional value of dietary proteins and related mechanisms. Therefore, this review seeks to give an updated discussion of the effects of oxidative modification on the structural characteristics and nutritional value of dietary proteins, and elucidate the interaction with gut microbiota, intestinal tissues, and organs. Additionally, the specific mechanisms related to pathological conditions are also characterized. Dietary protein oxidation during food processing and storage change protein structure, which further influences the in vitro digestion properties of proteins. In vivo research demonstrates that oxidized dietary proteins threaten body health via complicated pathways and affect the intestinal microenvironment via gut microbiota, metabolites, and intestinal morphology. This review highlights the influence of oxidative modification on the nutritional value of dietary proteins based on organs and the intestinal tract, and illustrates the necessity of appropriate experimental design for comprehensively exploring the health consequences of oxidized dietary proteins.

A solution structure analysis reveals a bent collagen triple helix in the complement activation recognition molecule mannan-binding lectin

Collagen triple helices are critical in the function of mannan-binding lectin (MBL), an oligomeric recognition molecule in complement activation. The MBL collagen regions form complexes with the serine proteases MASP-1 and MASP-2 in order to activate complement, and mutations lead to common immunodeficiencies. To evaluate their structure-function properties, we studied the solution structures of four MBL-like collagen peptides. The thermal stability of the MBL collagen region was much reduced by the presence of a GQG interruption in the typical (X-Y-Gly)n repeat compared to controls. Experimental solution structural data were collected using analytical ultracentrifugation and small angle X-ray and neutron scattering. As controls, we included two standard Pro-Hyp-Gly collagen peptides (POG)10-13, as well as three more peptides with diverse (X-Y-Gly)n sequences that represented other collagen features. These data were quantitatively compared with atomistic linear collagen models derived from crystal structures and 12,000 conformations obtained from molecular dynamics simulations. All four MBL peptides were bent to varying degrees up to 85o in the best-fit molecular dynamics models. The best-fit benchmark peptides (POG)n were more linear but exhibited a degree of conformational flexibility. The remaining three peptides showed mostly linear solution structures. In conclusion, the collagen helix is not strictly linear, the degree of flexibility in the triple helix depends on its sequence, and the triple helix with the GQG interruption showed a pronounced bend. The bend in MBL GQG peptides resembles the bend in the collagen of complement C1q and may be key for lectin pathway activation.

Temperature-Dependent Structure–Function Properties of Bacterial Xylose Isomerase Enzyme for Food Applications: An In Silico Study

Xylose Isomerase (XI) is an intramolecular oxidoreductase enzyme and catalyzes the reversible conversion of ketoses and aldoses in addition to the bioconversion of ethanol from xylose in the production of bioethanol from hemicellulose. It has a broad range of industrial applications in the food and pharmaceutical sectors, particularly in the production of the sweetener high fructose corn syrup (HFCS). It is one of the most widely used industrial enzymes after protease. Taking this into consideration, four bacterial XI sources were selected based on growth temperature, i.e., psychrophile, mesophile, thermophile, and hyperthermophile, for analyzing Xylose Isomerase’s structure-function characteristics. It was found that thermophilic XI was structurally less stable than mesophilic and hyperthermophilic XI, whereas structural plasticity ran opposite towards mesophiles. The interaction of xylose isomerase (XI) with two ligands, namely Amino-2-Hydroxymethyl-Propane-1,3-Diol and (4R)-2-Methylpentane-2,4- Diol, was also studied. Mesophilic XI demonstrated better binding affinity with structurally stabilizing amino acids (Ala, Asp, Gly, Leu, and Arg). In comparison, Thermophilic XI showed nearly similar binding affinity with both Amino-2-Hydroxymethyl-Propane-1,3-Diol and (4R)-2-Methylpentane-2,4-Diol. The results of this investigation suggest that thermophilic XI, followed by mesophilic XI, would be the most appropriate for establishing process stability and sustainability in the food industry.

Metal Oxide/Graphene/Metal Sandwich Structure for Efficient Photoelectrochemical Water Oxidation

AbstractSingle‐layer graphene (SLG) has drawn considerable interest in photoelectrochemical (PEC) cells due to its atomically flat pinhole‐free structure and remarkable in‐plane carrier mobility. It is challenging, however, to obtain efficient SLG‐modified photoelectrodes for PEC water splitting mainly due to the inefficient charge transfer interface. Here, a transition metal oxide/SLG/transition metal sandwich structure modified n‐Si‐based model photoanode is constructed to regulate the interfacial charge transfer behavior for enhanced PEC water oxidation performance. In this sandwich configuration, SLG tailors the morphology, structure, and work function properties of surface metal electrocatalysts to obtain both higher thermodynamic photovoltage and faster kinetical charge transfer at the semiconductor/electrolyte interface. In addition, SLG promotes the surface catalytic reaction as an effective charge trap and storage layer. This study provides a new structural design to engineer the SLG interfacial properties for high‐performance energy conversion devices.

Polymer Molecular Weight Determination via Fluorescence Lifetime.

Control of polymer molecular weight is critical for tailoring structure-function properties; however, traditional molecular weight characterization techniques have limited ability to determine the molecular weight of polymers in real time without sample removal from the reaction mixture, with spatial resolution, and of insoluble polymers. In this work, a fluorescence lifetime imaging microscopy (FLIM) method was developed that overcomes these limitations. The method is demonstrated with polynorbornene and polydicyclopentadiene, polymers derived from ruthenium-catalyzed ring-opening metathesis polymerization (ROMP). The polymer Mw, ranging from 35 to 570 kg/mol as determined by gel-permeation chromatography, was quantitatively correlated with the fluorescence lifetime. The revealed correlation then enabled time-resolved measurement of Mw during an ongoing ROMP reaction, requiring only 1 s per measurement (of a 45 μm × 45 μm polymer sample area), and provided spatial resolution, resulting in simultaneous characterization of polymer morphology. To provide the fluorescence signal, the initial reaction solutions contained a very low doping of a reactive norbornene monomer labeled with fluorescent boron dipyrromethene (BODIPY), such that 1 in every 107 monomers contained a fluorophore. The resulting FLIM visualization method enables the rapid determination of the molecular weights of growing polymers without removal from the reaction mixture and regardless of polymer solubility.

Structural and functional modification of proteins from black soybean Aquasoya via ultrasonication.

Plant-based proteins obtained from agricultural by-products have garnered growing interest in response to consumer awareness of health and environmental issues. This study aimed to improve the functionalities of the proteins recovered from black soybean Aquasoya (PBSA) by modifying their structure via ultrasonication. PBSA was ultrasonicated with a frequency of 40kHz at 350W for different time periods (0, 20, 40, and 60min), and its structural characteristics, physicochemical properties, and functional properties were investigated. Ultrasonication left the primary structure intact but altered the secondary and tertiary structures of the PBSA; α-helix and β-sheet contents decreased, random coil contents increased, and buried non-polar amino acid residues were exposed. Moreover, ultrasound promoted an increase in free sulfhydryl content and a reduction in particle size. Consequently, functional properties, such as solubility, emulsion stability, and foaming performance were improved by modifying the structure and physicochemical properties of PBSA. This work demonstrates the potential of ultrasound, which is favorable for modifying the spatial conformation and related functionalities of proteins, thus meeting the needs of manufacturers to use function-enhanced proteins as food additives.

Structural-Functional Properties of Asymmetric Fluoro-Alkoxy Substituted Benzothiadiazole Homopolymers with Flanked Chalcogen-Based Heterocycles.

The synthesis and characterization of asymmetric alkoxy- are reported, fluoro-benzothiadiazole (BT) acceptor core derivatized with a series of six different heterocycles (selenophene, thiophene, furan, 5-thiazole, 2-thiazole and 2-oxazole). The effect of the flanked-heterocycles containing different chalcogen atoms of the six homopolymers (HPX) is studied using optical, thermal, electrochemical, and computational analysis. Computational calculations indicate a strong relationship between the most stable conformation for each homopolymer and their bearing heterocycle, thus homopolymers HPSe', HPTp', HPFu', and HPTzC5, adopted the syn-syn and syn-anti conformations due to their noncovalent interactions with shorter distances, while HPTzC2' and HPOx' demonstrate preference for the anti-anti conformation. Optical property studies of the homopolymers reveal a strong red-shift in solution and film upon exchanging the chalcogen atom from Oxygen < Sulfur < Selenium in HPFu, HPTp, and HPSe, respectively. In addition, deeper highest occupied molecular orbital (HOMO) energy levels are observed when the donor-acceptor moieties (HPSe, HPTp, and HPFu) are substituted for the acceptor-acceptor systems such as HPTzC5, HPTzC2, and HPOx. Improved packing and morphology are exhibited for the donor-acceptor homopolymers. Thus, having a flanked heterocycle containing different chalcogen-atoms in polymeric systems is one way of tuning the physicochemical properties of conjugated materials for optoelectronic applications.

Submodular maximization of concave utility functions composed with a set-union operator with applications to maximal covering location problems

We study a family of discrete optimization problems asking for the maximization of the expected value of a concave, strictly increasing, and differentiable function composed with a set-union operator. The expected value is computed with respect to a set of coefficients taking values from a discrete set of scenarios. The function models the utility function of the decision maker, while the set-union operator models a covering relationship between two ground sets, a set of items and a set of metaitems. This problem generalizes the problem introduced by Ahmed S, Atamtürk A (Mathematical programming 128(1-2):149–169, 2011), and it can be modeled as a mixed integer nonlinear program involving binary decision variables associated with the items and metaitems. Its goal is to find a subset of metaitems that maximizes the total utility corresponding to the items it covers. It has applications to, among others, maximal covering location, and influence maximization problems. In the paper, we propose a double-hypograph decomposition which allows for projecting out the variables associated with the items by separately exploiting the structural properties of the utility function and of the set-union operator. Thanks to it, the utility function is linearized via an exact outer-approximation technique, whereas the set-union operator is linearized in two ways: either (i) via a reformulation based on submodular cuts, or (ii) via a Benders decomposition. We analyze from a theoretical perspective the strength of the inequalities of the resulting reformulations, and embed them into two branch-and-cut algorithms. We also show how to extend our reformulations to the case where the utility function is not necessarily increasing. We then experimentally compare our algorithms inter se, to a standard reformulation based on submodular cuts, to a state-of-the-art global-optimization solver, and to the greedy algorithm for the maximization of a submodular function. The results reveal that, on our testbed, the method based on combining an outer approximation with Benders cuts significantly outperforms the other ones.

Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities.

To understand the mechanisms of immunomodulatory effect, Dendrobium Officinale polysaccharides (DOP) were treated by ultrasound and mild base separately to generate fractions of various weight-average molecular weight (Mw) and degrees of acetylation (DA). The structural features, conformational properties, functional properties and immunomodulatory activities of original and modified DOPs were investigated. Ultrasonic treatment decreased the Mw and apparent viscosity and improved the water solubility of DOP. Mild base treatment remarkably reduced the DA and the water solubility, while the overall apparent viscosity was increased. Conformational analysis by triple-detector high performance size-exclusion chromatography showed that the molecular chain of DOP turned more compact coil conformation with decreased DA. Results from the macrophages RAW 264.7 analysis showed that samples sonicated for 200 min (Mw 34.2 kDa) showed the highest immune-regulation effects. However, the immunomodulatory effects of the samples after de-acetylation were all compromised compared to the original DOP. This study inspires further research to establish the structural-immunomodulatory relationships, which promote the application of DOP in both the food and medicine fields.

Characterization of light chain c-terminal extension sequence variant in one bispecific antibody.

Protein modifications such as post-translational modifications (PTMs) and sequence variants (SVs) occur frequently during protein biosynthesis and have received great attention by biopharma industry and regulatory agencies. In this study, an aberrant peak near light chain (LC) was observed in the non-reduced capillary electrophoresis sodium dodecyl sulfate (nrCE-SDS) electrophoretogram during cell line development of one bispecific antibody (BsAb) product, and the detected mass was about 944 Da higher than LC. The corresponding peak was then enriched by denaturing size-exclusion chromatography (SEC-HPLC) and further characterized by nrCE-SDS and peptide mapping analyses. De novo mass spectra/mass spectra (MS/MS) analysis revealed that the aberrant peak was LC related sequence variant, with the truncated C-terminal sequence “SFNR” (“GEC”deleted) linked with downstream SV40 promotor sequence “EAEAASASELFQ”. The unusual sequence was further confirmed by comparing with the direct synthetic peptide “SFNREAEAASASELFQ”. It was demonstrated by mRNA sequencing of the cell pool that the sequence variant was caused by aberrant splicing at the transcription step. The prepared product containing this extension variant maintained well-folded structure and good functional properties though the LC/Heavy chain (HC) inter-chain disulfide was not formed. Several control strategies to mitigate the risk of this LC related sequence variant were also proposed.