Robust Structure Research Articles

Leveraging Blood Components for 3D Printing Applications Through Programmable Ink Engineering Approaches.

This study proposes a tunable ink engineering methodology to allow 3D printing processability of highly bioactive but otherwise low-viscous and unprintable blood-derived materials. The hypothesis relies on improving the viscoelasticity and shear thinning behavior of platelet lysates (PL) and albumins (BSA) solutions by covalent coupling, enabling simultaneous extrusion and photocrosslinking upon filament deposition. The available amine groups on proteins (PL and BSA) are exploited for coupling with carboxyl groups present in methacrylated proteins (hPLMA and BSAMA), by leveraging carbodiimide chemistry. This reaction enabled the creation of a pre-gel from these extremely low-viscous materials (≈ 1Pa), with precise tuning of the reaction, resulting in inks with a range of controlled viscosities and elasticities. Shape-fidelity analysis is performed on 3D-printed multilayered constructs, demonstrating the ability to reach clinically relevant sizes (>2cm in size). After photocrosslinking, the scaffolds showcased a mechanically robust structure with sustained protein release over time. Bioactivity is evaluated using human adipose-derived stem cells, resulting in increased viability and metabolic activity over time. The herein described research methodology widens the possibilities for the use of low-viscosity materials in 3D printing but also enables the direct application of patient and blood-derived materials in precision medicine.

Raman spectroscopy reveals oxidative stress-induced metabolic vulnerabilities in early-stage AR-negative prostate-cancer versus normal-prostate cell lines

Quantitative Raman spectroscopy provides information-rich imaging of complex tissues. To illustrate its ability to characterise early-stage disease, we compared live P4E6, a low-grade Gleason-3 prostate-cancer cell line, to PNT2-C2, a normal prostate cell-line equivalent, thereby elucidating key molecular and mechanistic differences. Spectral changes from statistically relevant population sampling show P4E6 is defined by reduced DNA/RNA signatures (primarily base-pair modifications), increased protein-related signatures (synthesis), decreased whole-cell measured saturated and unsaturated fatty acids, and increased cholesterol and cholesterol ester (lipid storage). Signatures in the live-cell disease state point to the Warburg effect for aerobic glycolysis as the mechanism for cellular energy generation. A follow-on study involving catastrophic desiccation showed a key survival pathway in the cancer state in the structural robustness of DNA/RNA. Metabolic changes, namely in Warburg-to-oxidative-phosphorylation rerouting and reduced protein synthesis, were also shown. Such modifications limit cancer’s resistance to oxidative damage, and thus its ability to utilise a higher redox homeostasis for metabolic advantage. The results demonstrate the ability of quantitative Raman spectroscopy to uncover, with full molecular-heterogeneity capture, mechanistic vulnerabilities in lowest-grade tumorigenic prostate cancer, thereby revealing underlying targets for disease disruption at early stage.

Precise Single‐Atom Modification of Hybrid Lead Chlorides for Electron Donor‐Acceptor Effect and Enhanced Photocatalytic Aerobic Oxidation

Hybrid lead halides show significant potential in photocatalysis due to their excellent photophysical properties, but the atomically precise modification of their organic component to achieve synergistic interactions with the lead halide units remains a great challenge. Herein, for the first time, we have employed the crystal engineering strategy to construct a class of single‐atom‐substituted hybrid lead halides with electron donor‐acceptor (D‐A) effect. The lead halide frameworks consist of 1D linear [PbCl]+ chains as inorganic building units and benzoxadiazole/benzothiadiazole/ benzoselenadiazole‐funtionalized dicarboxylates as linkers. The covalent bonding between the organic ligands with electron‐withdrawing groups and the electron‐rich lead halide units not only facilitate the charge separation, but also enhance structural robustness that is critical for photocatalysis. The D‐A structured lead halides serve as highly efficient heterogeneous photooxidation catalysts, including aerobic oxidation of C(sp3)‐H bonds, oxidative coupling of primary amines, oxidation of phenylboronic acids and selective oxidation of sulfides that are demonstrated in 30 examples. Importantly, these photooxidation reactions are able to be driven by natural sunlight and ambient air to afford quantitative yields. Moreover, our lead halide photocatalysts are successful to fix into a photocatalytic flow system, which enables the flow‐type synthesis of high value‐added photooxidation products on a gram scale.

Precise Single-Atom Modification of Hybrid Lead Chlorides for Electron Donor-Acceptor Effect and Enhanced Photocatalytic Aerobic Oxidation.

Hybrid lead halides show significant potential in photocatalysis due to their excellent photophysical properties, but the atomically precise modification of their organic component to achieve synergistic interactions with the lead halide units remains a great challenge. Herein, for the first time, we have employed the crystal engineering strategy to construct a class of single-atom-substituted hybrid lead halides with electron donor-acceptor (D-A) effect. The lead halide frameworks consist of 1D linear [PbCl]+ chains as inorganic building units and benzoxadiazole/benzothiadiazole/ benzoselenadiazole-funtionalized dicarboxylates as linkers. The covalent bonding between the organic ligands with electron-withdrawing groups and the electron-rich lead halide units not only facilitate the charge separation, but also enhance structural robustness that is critical for photocatalysis. The D-A structured lead halides serve as highly efficient heterogeneous photooxidation catalysts, including aerobic oxidation of C(sp3)-H bonds, oxidative coupling of primary amines, oxidation of phenylboronic acids and selective oxidation of sulfides that are demonstrated in 30 examples. Importantly, these photooxidation reactions are able to be driven by natural sunlight and ambient air to afford quantitative yields. Moreover, our lead halide photocatalysts are successful to fix into a photocatalytic flow system, which enables the flow-type synthesis of high value-added photooxidation products on a gram scale.

Powerful graph neural network for node classification of the IoT network

Internet of Things (IoT) devices are increasingly used in various applications in our daily lives. The network structure for IoT is heterogeneous and can create a complex architecture depending on the application and geographical structure. To efficiently process the information within this diverse and complex relationship, a robust data structure is needed for network operations. Graph neural network (GNN) technology is emerging as a capable tool for predicting complex data structures, such as graphs. Graphs can be employed to mimic the structure of IoT network and process information from IoT nodes using GNN techniques. In this paper, our goal is to explore the effectiveness of GNN in performing the node classification task for a given IoT network. We have generated three different IoT networks with varying network sizes, number of nodes, and feature sizes. We then test 12 different GNN algorithms to evaluate their performance in IoT node classification. Each method is examined in detail to observe its training behavior, testing behavior, and resilience against noise. In addition, time complexity and generalization ability of each model have also been studied. The experimental results show that some methods exhibit high resilience against noisy data for IoT node classification accuracy.

Facile synthesis of monoclinic (NH4)2V4O9 nanosheets for zinc-ion batteries

Aqueous zinc-ion batteries (ZIBs) are increasingly popular due to their low cost and high safety. However, the quest for cathode materials with robust internal structures and efficient ion transport pathways remains critical. In this study, we introduce a simple hydrothermal method to synthesize monoclinic (NH4)2V4O9 nanosheets, serving as a advanced cathode for ZIBs. This material demonstrates outstanding electrochemical properties, with a high specific capacity of 389 mAh g−1 at 0.1 A g−1, and retains 78.9 % capacity after 3000 cycles at 1 A g−1 and 77.3 % after 15,000 cycles at 5 A g−1. Additionally, it achieves an energy density of 325 Wh kg−1 at 101 W kg−1. In-depth electrochemical analysis reveals the structural stability during reversible Zn2+ ion intercalation/de-intercalation, underscoring the potential of (NH4)2V4O9 as a high-performance material for ZIBs.

Investor relations and corporate governance in the global energy sector: A theoretical review of best practices and their application in emerging economies

Investor relations and corporate governance are critical components in ensuring the sustainable development and operational efficiency of companies within the global energy sector. This theoretical review explores the best practices in investor relations and corporate governance, particularly focusing on their application within emerging economies. The global energy sector is highly capital-intensive, requiring robust governance structures to manage risks, ensure transparency, and align the interests of stakeholders. Effective investor relations strategies foster trust, provide timely and accurate information, and enhance shareholder engagement, all of which are essential for securing long-term investments in energy projects. Corporate governance, on the other hand, ensures accountability, compliance with regulatory frameworks, and ethical management practices. The review highlights best practices in corporate governance, including the establishment of independent boards, strong leadership, risk management frameworks, and the importance of environmental, social, and governance (ESG) criteria in decision-making processes. In terms of investor relations, strategies such as proactive communication, regular financial disclosures, and engagement with both institutional and retail investors are identified as key to maintaining investor confidence. In emerging economies, where energy needs are rapidly increasing, the application of these best practices presents both challenges and opportunities. Regulatory frameworks in these regions are often evolving, and companies must navigate complex political and economic environments while adhering to global standards. This review discusses how emerging markets can benefit from adopting globally recognized governance and investor relations practices to attract foreign investment and foster sustainable energy development. The theoretical insights presented offer a comprehensive understanding of the interplay between investor relations and corporate governance in the energy sector. Additionally, this review underscores the importance of adaptability and innovation in applying these best practices to the unique contexts of emerging economies.. Keywords: Investor Relations, Corporate Governance, Global Energy Sector, Emerging Economies, Best Practices, ESG Criteria, Stakeholder Engagement, Transparency, Risk Management, Sustainable Energy Development.

Evidence of community structure in phonological networks of multiple languages.

Thousands of phonological word forms known to a speaker can be organised as a lexical network using the tools of network science. In these networks, nodes represent words and edges are placed between phonological neighbours. Previous work has shown that phonological networks of various languages have similar macrolevel network properties. The present study aimed to investigate if phonological networks of different languages also have similar mesolevel properties, specifically, the presence of robust community structure. Prior community detection analyses revealed robust community structure for English. Community detection analyses conducted on French, German, Dutch, and Spanish networks indicate that all networks showed strong evidence of community structure-mesolevel clustering of word forms whereby larger communities tended to contain shorter, frequent words with many phonological neighbours. Words of the same community tended to share similar phonotactic structures. Results suggest that the organisation of phonological word forms in language are governed by similar principles that could have important implications for lexical processing. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Structure and sexual dimorphism of the first antennae in Penaeus vannamei, Boone, 1931

ABSTRACT This contribution is a histological and scanning electron microscopy study of major flagellar setae of antennules from the most cultured crustacean species, Penaeus vannamei. Antennule samples were collected from females and males of selected species and processed for histological as well as scanning electron microscopy (SEM) evaluation. The histological and SEM description of major setae from antennules, namely aesthetascs, plumose setae, and male spines are presented in detail. A unique sexually dimorphic structure, male spines on the inner (medial) flagella, is reported for the first time in a penaeid shrimp. These spines are robust structures, short and thick with non-permeable cuticle and no terminal pore, innervated by receptor neuron clusters. This histological organization suggests the spines are sensilla for mechanoreception, possibly involved in courtship mechanoreception, complementing chemosensory information from aesthetascs. Additionally, SEM observations of other antennule setae are presented, including asymmetric setae associated with aesthetascs.

In Situ Molecular Reconfiguration of Pyrene Redox-Active Molecules for High-Performance Aqueous Organic Flow Batteries.

Aqueous organic flow batteries (AOFBs) hold great potential for large-scale energy storage, however, scalable, green, and economical synthetic methods for stable organic redox-active molecules (ORAMs) are still required for their practical applications. Herein, pyrene-based ORAMs are obtained via an in situ organic electrolysis strategy in a flow cell. It is revealed that the water attacking pyrenes restructured molecules to produce a variety of isomers and dimers during the electrolysis, which can be modulated by regulating the local electron cloud density and steric hindrance of pyrene precursors. As a result, the molecularly reconfigured pyrene-based catholytes, even without any further purification, achieved a high electrolyte utilization of ≈96% and volumetric capacity above 50AhL-1. Inspiringly, remarkable cell stability with almost no capacity decay for ≈70 days is achieved, benefiting from the robust aromatic structure of the pyrene cores. The insights into the in situ electrosynthesis of pyrene-based ORAMs provided in the work will provide guidance for designing ultra-stable ORAMs for AOFB applications.

Effect of Temperature on Intermediate Phases of Na3V2(PO4)3 during Cycling by Operando X-ray Diffraction.

Na3V2(PO4)3 (NVP) has gained a lot of attention due to its remarkable properties, such as its robust crystal structure, cycle life, rate capabilities, and so on. Nevertheless, NVP undergoes a substantial decrease in its rate capability at low temperatures, which limits its practical applications. In this study, the performance of NVP at low, room, and high temperatures during cycling is thoroughly investigated using synchrotron operando X-ray diffraction. The (de)insertion of two sodium ions from Na3V2(PO4)3 to Na1V2(PO4)3 appeared to occur via two intermediate phases (Na2V2(PO4)3 and Na1.64V2(PO4)3). The Na1.64V2(PO4)3 phase which is observed for the first-time during operando XRD measurements of NVP, exhibited limited stability at high temperatures. The increase in the quantity of these intermediate phases from high to low temperatures, especially at high C-rates, could be anticipated to be one of the contributing factors of poor rate capabilities of NVP at low temperatures. This study encourages the exploration of suitable strategies to enhance the performance of NVP at low temperatures and high C-rates.

Design and evaluation of a hybrid offshore wave energy converter and floating photovoltaic system for the region of Oran, Algeria

Introduction. This paper presents the novel design and analysis of a hybrid renewable energy system that combines a wave energy converter (WEC) with a floating photovoltaic (FPV) system for offshore installation, with a specific focus on Oran as a case study. The purpose of integrating these two technologies is to harness both wave and solar energy, thereby maximizing energy output and enhancing the reliability of renewable energy sources in offshore environments. The goal of this study is to develop a hybrid system that leverages the complementary nature of WEC and FPV technologies to maximize energy output and improve reliability. By integrating these technologies, the system aims to overcome the limitations of standalone energy systems. The methodology includes selecting suitable WEC and FPV technologies, optimizing their configurations, and analyzing their combined performance under various environmental conditions. To assess the energy production potential, structural stability, and economic feasibility of the hybrid system, computational simulations and data analysis are employed. This comprehensive approach ensures rigorous testing and optimization for real-world applications. The results demonstrate substantial improvements in energy yield and system resilience compared to standalone WEC or FPV systems. The hybrid system shows enhanced performance, particularly in consistent energy output and structural robustness. These findings indicate that combining WEC and FPV technologies can lead to more reliable and efficient offshore renewable energy solutions. The practical values are significant, providing insights into efficient and sustainable offshore renewable energy solutions. By focusing on Oran, it offers a localized perspective that can be adapted to similar coastal areas globally, contributing to the advancement of renewable energy technologies. The hybrid system’s enhanced reliability and efficiency support the broader goal of sustainable energy development in marine environments, highlighting its potential for widespread application and impact. References 23, tables 4, figures 17.

Highly Filled Waste Polyester Fiber/Low-Density Polyethylene Composites with a Better Fiber Length Retention Fabricated by a Two-Rotor Continuous Mixer

A key challenge in the utilization of waste polyester fibers (PET fibers) is the development of fiber-reinforced composites with high filler content and the improvement of fiber length retention. Herein, the effects of a two-rotor continuous mixer and a twin-screw extruder on the structure and properties of waste polyester fiber composites were evaluated. The results revealed that the mechanical properties of the composites were improved significantly with increasing fiber content, especially when processed using the twin-rotor continuous mixer. This mixer facilitated the formation of a robust fiber network structure, leading to substantial enhancements in tensile strength, flexural strength, and heat resistance. Specifically, compared to those processed by the twin-screw extruder, with 60 wt% fibers content, the tensile and flexural strengths of specimens processed by the twin-rotor continuous mixer increase by 21% and 13%, respectively. The average fiber length in specimens processed by the twin-rotor continuous mixer was 32% longer than that in specimens processed by the twin-screw extruder, attributable to the lower shear frequency and the higher tensile ratio of the former. This blending technique emerges as an effective strategy, contributing significantly to promoting the development and practical application of waste textile fiber-reinforced polymer composites.

ZrO2 nanoparticle embedded reusable and self-standing biopolymeric membrane for efficient piezodynamic eradication of gram-positive and gram-negative coliform bacteria

Bacterial infections pose a significant global threat, especially with the increasing prevalence of antibiotic resistance and multidrug resistance (MDR). To address this challenge, dynamic antibacterial therapies are becoming increasingly important, with piezodynamic therapy emerging as a particularly promising approach due to its non-invasive nature and quick response using mechanical stimuli to target bacterial contaminations. This study, probably for the first-time, introduced an advanced piezo-active membrane, ZrO2 nanoparticle-loaded chitosan biopolymer (ZO@CHS), which utilized piezodynamic therapy to combat both Gram-positive (E. faecalis) and Gram-negative (E. coli) coliform bacteria. By combining the biocompatibility of chitosan with the enhanced physiochemical properties of ZrO2, this self-supporting and non-toxic highly polarized bio-nanocomposite (ZO@CHS) membrane generated a significant piezo-voltage of 4.47 V upon finger tapping and promoted cellular adhesion while enhancing reactive radicals’ production under mild ultrasound (∼15 kHz), eliminating over 96 % of E. coli and 97 % E. faecalis within just 20 minutes, which corroborated well with the statistical analysis. Detailed study, including reactive oxygen species (ROS) assessment through Photoluminescence spectroscopy and bacterial FESEM examination, provided insights into the underlying mechanism. Additionally, the membrane’s robust structure and hydrophobic properties allow for cyclic reuse, retaining over 95 % effectiveness after five cycles. Furthermore, the superior biocompatibility of the membrane, evidenced by a minimal haemolysis rate of 0.11 %, making it suitable for potential in vivo applications. Thus, this novel membrane presented promising applications in healthcare systems and pigment industries, serving as a self-cleaning material.

Synergising expertise towards sustainability and robustness of cement-based materials and concrete structures

Synergising expertise towards sustainability and robustness of cement-based materials and concrete structures

Enabling and inhibiting factors of sustainability of Farmer's Producers Organisations in India

Farmer Producer Organisations (FPOs) are farmers' collectives or farmer-led business entities seeking to empower small and marginal farmers in India. Some farmer's collectives successfully achieve their goals and some often fail to meet the increasing demands in the agribusiness ecosystem. However, the academic literature glorifies the success studies of farmers' collectives. There are mentions of sustainability issues of farmers' collectives. However, not enough studies point to the factors that influence the organisational sustainability of farmers' collectives. Therefore, in this paper, the researchers aim to explore both the enabling and inhibiting factors of sustainability of farmer's collectives. A thematic synthesis process was applied to compile information from published literature. The study compiles enabling and inhibiting sustainability factors from 22 studies of FPOs. A qualitative approach using focused group discussion with farmers in FPO was completed to validate the findings from secondary data sources and collect empirical evidence directly from the farmers. The methodology guarantees inclusivity by collecting the voices of farmers through focused group discussion. Key enabling and inhibiting factors of sustainability of FPOs were categorized into economic, social, and environmental dimensions of organisational sustainability. In the economic dimension, improved agricultural income of farmers was reported as an enabling factor for sustainability, while some FPOs report capital shortage as a main hindrance for organisational sustainability in FPOs. The social dimension claims the collective bargaining power of members and effective capacity-building and collaboration opportunities as enabling factors. However, farmers highlight without good leadership strategies, and upskilling of farmers attaining sustainability is a mirage. Effective organisational activities like branding, marketing of products, access and awareness about capacity building were derived as the enabling factors in the environmental dimension of organisational sustainability, whereas, poor infrastructure lack of robust organisational structure, and climate change were termed as inhibiting factors. Understanding these sustainability factors can help policymakers to develop farmer-friendly collectives which enhance agriculture and food systems that will help achieve Sustainable Development Goal (SDG) 1—Zero Poverty and SDG 2—End hunger.

Synergistic effects of deep eutectic solvents on the morphology and performance of polysulfone ultrafiltration membranes

This study investigates the synthesis of flat sheet asymmetric Polysulfone (PSF) membranes using the Non-Solvent Induced Phase Separation (NIPS) method, enhanced by incorporating Deep Eutectic Solvents (DES) composed of Choline Chloride (ChCl) and DL-Malic Acid (MA). The research explores the individual and combined effects of ChCl and MA on membrane morphology and performance. Comprehensive characterization techniques, including Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy-Universal Attenuated Total Reflectance (FTIR-UATR), and Atomic Force Microscopy (AFM), were employed to analyze the structural and surface properties of the membranes. Key performance metrics such as Pure Water Permeability (PWP), protein and dye rejection, fouling behavior, porosity, surface hydrophilicity, and mechanical strength were evaluated. Results demonstrated that integrating DES into the PSF matrix significantly improved membrane properties. The 3% DES membrane exhibited the highest Pure Water Permeability (PWP) of 186.82 L/m2h/bar, the lowest water contact angle of 68.8°, and optimal balance in surface roughness parameters, leading to superior antifouling properties with high flux recovery ratio (FRR) and balanced reversible (Rr) and irreversible fouling (Rir) components. The ChCl (HBA) membrane displayed a notable PWP of 121.62 L/m2h/bar, large pore sizes (42.72 nm), and moderate surface roughness (Ra of 3.32 nm). In contrast, the MA (HBD) membrane demonstrated the highest hydrophilicity with the lowest contact angle (70.7°) and a compact, robust structure, despite its smallest pore sizes and lack of permeability. The findings underscore the synergistic effect of DES formation in the membrane, improving overall performance for ultrafiltration applications. This study provides valuable insights into the distinct roles of ChCl as an HBA and MA as an HBD in DES-modified PSF membranes, revealing their individual contributions and the importance of optimizing DES components and concentrations for specific filtration applications.

Border Gateway Protocol Route Leak Detection Technique Based on Graph Features and Machine Learning

In the Internet, ASs are interconnected using BGP. However, due to a lack of security considerations in the design of BGP, a series of security issues arise during the propagation of routing information, such as prefix hijacking, route leakage, and AS path tampering. Therefore, this paper conducts research on the detection of route leakage. By analyzing BGP routing information, we abstract the routing propagation relationship between ASs into a network topology graph, and extract graph features from the graph abstracted from routing data at certain time intervals. Based on the structural robustness features and centrality measurement features of the graph, we determine whether a route leakage has occurred during the current time period. To this end, we use machine learning methods and propose a weighted voting model. This model trains multiple single models and assigns weights to them, and through the weighted analysis of the results of multiple models, it can determine whether a route leakage has occurred. In addition, to determine the corresponding weights, we use genetic algorithms for identifying route leaks. The experimental results show that the method used in this paper has a high accuracy rate, and compared with a single model, it performs better on multiple datasets.

Crisis management from a relational perspective: an analysis of interorganizational transboundary crisis networks

Abstract Although transboundary crises have gained relevance in an increasingly interdependent world, our understanding of the relational dynamics governing these phenomena remains limited. This paper addresses this knowledge gap by identifying common characteristics across interorganizational transboundary crisis networks and drivers of tie formation in successful structures. For this purpose, it applies descriptive Social Network Analysis and Exponential Random Graph Models to an original dataset of three networks. Results show that these structures combine elements of issue networks and policy communities. Common features include moderately high centralization, reciprocated ties, core-periphery structures, and the popularity of international organizations. Additionally, successful networks display smooth communication between NGOs and international organizations, whereas unsuccessful networks have fewer heterophilous interactions. Transitivity seems to play a role in network success too. These findings suggest that crisis networks are robust structures that reconcile bridging and bonding dynamics, thereby highlighting how evidence from relational studies could guide transboundary crisis management.

Rutile-phase Sn-Ti Mixed Oxides as Acid Catalysts for the Condensation of C2-C3 Oxygenated Compounds in Water.

The design of new cost-effective and water-resistant acid catalysts is crucial for valorizingaqueous side-streams to increase the efficiency and competitiveness of bio-refineries in a sustainable way. In this work, Sn-Ti mixed oxides are prepared via a green and scalable co-precipitation method. This synthesis procedure allows obtaining homogeneous rutile-phase Sn-Ti mixed oxides with enhanced textural properties and a higher amount of Lewis acid sites. More importantly, the catalytic behavior of these Sn-Ti mixed oxides is investigated by using an aqueous mixture of C2-C3 representative oxygenated compounds, closer to real industrial conditions and differing from usual individual probe molecules studies performed even in the absence of water. Catalytic experiments combined and correlated with spectroscopic measurements (i.e., XRD, TEM, FT-IR with pyridine adsorption-desorption and N2 adsorption) are performed to better understand the properties of different Sn-Ti catalysts. These materials show promising results in the condensation of light oxygenated compounds present in aqueous fractions. Homogeneous and robust rutile-phase structures with inherent hydrophobic characteristics turn these Sn-Ti materials into active and highly resistant catalysts capable to transform these low-value oxygenated compounds into a mixture of hydrocarbons and aromatics useful for blending with automotive fuels, especially under complex acidic aqueous environments and moderate process conditions.