In this work, we describe the biological method are used for the production of silver nanoparticles using plant extract. Silver (Ag) nanostructures were successfully prepared by a simple, highly efficient, and low-cost using the hydrothermal method by using the mint extract and evaluate their antimicrobial activity. The resulting nanostructures were characterized by XRD, FESEM, and UV-VIS spectroscopy. The nanoparticles structural properties were studied using X-Ray diffraction (XRD) and showed all the diffraction peaks are indexed to (F.C.C) structure. The crystallite size of Ag NPs was calculated and equal to 25 nm. FE-SEM images of silver showed nanoparticles that assembled in flower-like shape with a diameter of 10 nm-20 nm. The optical absorption explained by UV-Visible spectroscopy, the Ag NPs has sharp absorbance with the highest peak at 400 nm. The optical transmittance of the Ag film deposit time was around 40% at wavelength 400 nm then increases sharply at wavelength 400-900 nm. The energy gap increase to 3.4 eV. The antimicrobial activity was evaluated by agar well disc diffusion method against various microorganisms. The zone of inhibition against (Escherichia coli) was 20 mm, and fungus (Bacillus subtilis) was 25 mm. The use of silver nanoparticles in drug delivery systems might be the future thrust in the field of medicine.