The advancement of biomaterials for reconstruction and replacement of damaged tissues is essential, which would possess biocompatibility, better mechanical, biochemical, and physical properties. Merwinite (Ca3MgSi2O8) has emerged as a promising material for hard tissue engineering, particularly in composite with hydroxyapatite (HAp). Strontium-doped hydroxyapatite (SrHAp) was prepared with varying dopant concentrations (1%, 3%, and 5% Sr) on the calcium site of HAp. The materials were analyzed by XRD, FTIR and SEM/EDX techniques. XRD analysis revealed average crystallite sizes ranging from 46 to 50 nm for SrHAp. It was found that the Merwinite-SrHAp composite doped with 5% strontium had a higher rate of apatite nucleation compared to pure composite, indicating that strontium doping enhances the bioactivity of the composite. The composite scaffolds, prepared in an 80:20 ratio of SrHAp to merwinite, exhibited compressive strengths ranging from 63.43 MPa (1% Sr doping) to 80.68 MPa (5% Sr doping). Cytotoxicity assessment against MG63 cell line via MTT assay demonstrated cell inhibition rates ranging from 11.71% (at 6.5 μg/mL) to 68.35% (at 100 μg/mL) for the 5% Sr-doped composite. Alkaline phosphatase (ALP) investigation revealed enhanced ALP activity for cells cultured on the 5% Sr-doped composite compared to control samples, indicating increased cell differentiation and mineralization potential.