Strontium-Doped Hydroxyapatite Nanowires with a Hierarchical Interface: Enhancing Osteoinduction and Cellular Attachment

Abstract

Strontium-doped hydroxyapatite nanowires with hierarchical interfaces were prepared in this study by a one-step hydrothermal method using Pluronic123 as a structural guide. The cytotoxicity and biocompatibility of the materials were detected by the MTT assay. The adhesion and growth of MG63 (osteosarcoma cells) on materials with different doping ratios were observed by scanning electron microscopy and fluorescence microscopy. Real-time polymerase chain reaction (RT-PCR) was used to detect the transcription levels of alkaline phosphatase (ALP), Runt-associated transcription factor 2 (Runx2), osteopontin (OPN), type I collagen (Coll-I) and osteocalcin (OCN). The protein expression levels of ALP, Runx2, OPN, Col-I and OCN were detected by western blotting (WB). The results showed that strontium-doped hydroxyapatite nanowires had good biocompatibility and osteogenic induction ability, and the strontium-doped hydroxyapatite nanowires with a 1% strontium content exhibited a better osteogenic induction performance. Therefore, it is expected to become a new, promising bone induction scaffold material.