Strong Inhibition Zone Research Articles

Bioactive potential of Aphanamixis polystachya seed extracts

Aphanamixis polystachy seed extracts were examined for their in-vitro biological activities. The n-hexane and methanol extract showed significant phenolic content of 12.0 and 11.7 mg GAE/g, respectively. Antioxidant activity was exhibited by the methanol extract (IC50: 22.34 μg/mL) and moderate activity was shown by the n-hexane extract (IC50: 63.05 μg/ml) compared to BHT (tert-butyl-1- hydroxy toluene, IC50: 14.01 μg/ml) though both extracts caused moderate clot lysis of 18.84% and 23.49% compared to the positive control Streptokinase (65.16%). The n-hexane and methanol extract had shown 18.2% and 32.2% inhibition of hypnotic solution induced haemolysis respectively, while the n-hexane extract had almost no activity (2.21%) and methanol extract showed very high (70.17%) inhibition of heat induced haemolysis compared to acetyl salicylic acid (71.9%). Sensible and substantial cytotoxicity was exhibited by the n-hexane (LC50:13.45 μg/ml) and methanol extracts (LC50:1.61 μg/ml), respectively compared to vincristine sulfate (LC50: 0.45 μg/ml). The n-hexane extract showed no antimicrobial activity against the tested micro-organisms but the methanol extract showed a strong zone of inhibition (25 mm) against Staphylococcus aureus and a moderate zone of inhibition (10mm) against Shigella boydii. Both the extracts exhibited almost zero haemolysis.
 Bangladesh J. Sci. Ind. Res.56(2), 75-86, 2021

Phytochemical Screening, Antibacterial and Radical Scavenging Activities of Root Wood and Root Bark Extracts of Albizia schimperiana

Objective: Different parts of Albizia schimperiana are used for treatment of different types of diseases. In Ethiopia traditionally people use Albizia schimperiana to treat headache and other pains. In Tanzania the stem bark is used to treat warts, the leaf has shown significant antimicrobial and potential anti-helmintic activity. The main objective of the study was to identify the phytochemical constituents, and evaluate antibacterial and radical scavenging activities of root wood and root bark extracts of A. schimperiana. Method: The root of A. schimperiana was collected from east wollega zone, Nekemte town, in May 2018. The collected plant root was washed with distilled water and peeled so that the root wood and root bark were dried separately under shade at room temperature and ground in to powder using analytical mill. The bioactive constituents of the root of A. schimperiana were extracted through maceration technique using n-hexane, ethyl acetate, acetone, and methanol. The antibacterial assay was determined using disc diffusion method against four bacteria strains while the antioxidant activity of the plant extracts were investigated spectrophotometrically using DPPH. Result: The phytochemical screening revealed the presence of alkaloids, phenolic compounds, saponins, flavonoids, anthraquinones and terpenoids and absence of tannins and steroids in both root parts. The root extracts were active against all the tested bacterial strains. The root wood extracts of acetone and ethyl acetate demonstrated strong zone of inhibition against S. aureus (30mm) and E. coli (30 mm) at 100 mg/mL, as compared to the reference antibiotic gentamicin (20 mm). The methanol root wood extract of A. schimperiana has shown better DPPH radical scavenging activity at 1 mg/mL (89.8%) when compared to the methanol root bark extract (71.6%) at the same concentration. Conclusion: It can be concluded from this study that the root extracts of A. schimperiana owned many phytochemicals that exhibited promising antibacterial and antioxidant properties. Therefore, the root of A. schimperiana can serve as a potential source for development of new drugs as well as finding natural antioxidants that could assist the body in fighting disease like cancers.

Formulation and Antibacterial Activity Test of Foot Spray with Beluntas Leaf Ethanol Extract (Pluchea Indica L.)

Foot odor is one of the things that is quite disruptive to appearance, the 2014 American Podiatric Medical Association studied a significant increase in the prevalence of foot odor in the community. One of the causes of foot odor is the presence of bacteria, especially the bacteria Bacillus Subtilis. This study used the ethanol extract of beluntas leaf (Pluchea Indica L.) as the active ingredient because the ethanol extract of beluntas leaf has been widely used and proven to have antibacterial activity besides the ethanol extract of beluntas leaf has a high enough compatibility to be used as a cosmetic product, including spray preparations. The spray form was chosen because of its easy and pleasant use. This study aims to make a foot odor control product in the form of a spray with 3 concentrations of beluntas leaf ethanol extract, 0.5%, 1.0% and 1.5% to study the antibacterial activity using the well diffusion method. The results showed that the ethanol extract of beluntas leaf can be formulated into foot spray preparation with the results of the organoleptic examination of the three formulas are clear liquid, and yellow slightly green color. The pH of the preparation is in the range 5.10 -5.92. In formula 3 (F3) with a concentration of 1.5% beluntas leaf ethanol extract, it has a strong bacterial inhibition zone with an average inhibition zone diameter of 12.5 mm.

Gel formulation and evaluation of antimicrobial activity of turmeric (Curcuma longa L.) from Aceh, Indonesia against Aggregatibacter actinomycetemcomitans

Aim: Periodontitis is a chronic disease of dental tissue caused by a bacterial infection, for instance, Aggregatibacter actinomycetemcomitans is caused by dominant bacteria. Currently, there are many cases regarding bacterial resistance to some antibiotics, so an alternative search for new antibiotics from herbal plants is needed. Turmeric (Curcuma longa Linn.) is a plant that is known to contain bioactive compounds that can function as antimicrobials. So far, research on turmeric extract in the form of a gel formula used as a herbal medicine for periodontitis in Indonesia has not been reported. The aim of this research was to study the oral gel formula of the indigenous turmeric extract from Aceh, Indonesia and to evaluate its antimicrobial efficacy against A. actinomycetemcomitans. Materials and Methods: This research was designed to test the effectiveness of C. longa gel in vitro in inhibiting the growth of A. actinomycetemcomitan. Turmeric rhizome was extracted using the maceration method with 96% ethanol; then, the extract was formulated into a gel with concentrations of 0%, 25%, 50%, 75%, and 100%. The curcuma gel was then tested for antimicrobials against A. actinomycetemcomitans bacteria. Results: The in vitro antimicrobial test showed that a 50% concentration of turmeric rhizome extracts gel was effective in inhibiting the growth of A. actinomycetemcomitans with an inhibition zone of 10.8 mm, which is classified as a strong inhibition zone. Conclusion: The efficacy of the oral gel formula containing C. longa extracts as a natural antimicrobial agent has been confirmed, and the possibility of applying it as periodontitis herbal medicine has been suggested.

Phytochemical Screening, Antibacterial and Radical Scavenging Activities of Root Wood and Root Bark Extracts of Albizia schimperiana

Objective: Different parts of Albizia schimperiana are used for treatment of different types of diseases. In Ethiopia traditionally people use Albizia schimperiana to treat headache and other pains. In Tanzania the stem bark is used to treat warts, the leaf has shown significant antimicrobial and potential anti-helmintic activity. The main objective of the study was to identify the phytochemical constituents, and evaluate antibacterial and radical scavenging activities of root wood and root bark extracts of A. schimperiana. Method: The root of A. schimperiana was collected from east wollega zone, Nekemte town, in May 2018. The collected plant root was washed with distilled water and peeled so that the root wood and root bark were dried separately under shade at room temperature and ground in to powder using analytical mill. The bioactive constituents of the root of A. schimperiana were extracted through maceration technique using n-hexane, ethyl acetate, acetone, and methanol. The antibacterial assay was determined using disc diffusion method against four bacteria strains while the antioxidant activity of the plant extracts were investigated spectrophotometrically using DPPH. Result: The phytochemical screening revealed the presence of alkaloids, phenolic compounds, saponins, flavonoids, anthraquinones and terpenoids and absence of tannins and steroids in both root parts. The root extracts were active against all the tested bacterial strains. The root wood extracts of acetone and ethyl acetate demonstrated strong zone of inhibition against S. aureus (30mm) and E. coli (30 mm) at 100 mg/mL, as compared to the reference antibiotic gentamicin (20 mm). The methanol root wood extract of A. schimperiana has shown better DPPH radical scavenging activity at 1 mg/mL (89.8%) when compared to the methanol root bark extract (71.6%) at the same concentration. Conclusion: It can be concluded from this study that the root extracts of A. schimperiana owned many phytochemicals that exhibited promising antibacterial and antioxidant properties. Therefore, the root of A. schimperiana can serve as a potential source for development of new drugs as well as finding natural antioxidants that could assist the body in fighting disease like cancers.

Bioprospecting of marine diatoms Thalassiosira, Skeletonema and Chaetoceros for lipids and other value-added products

Diatoms are a major storehouse of valuable fucoxanthin and polyunsaturated fatty acids, with enormous nutraceuticals and biofuel potential. Three marine diatom species isolated from the southern coast of India has been screened and their results show that highest biomass concentration and fucoxanthin yield was obtained in Chaetoceros sp. as 0.217 g L−1 and 0.403 mg L−1 respectively. Lipid % as dry cell weight was maximum in Thalassiosira sp. (52%) followed by Skeletonema sp. (44%) and Chaetoceros sp. (22%). However, protein and secondary metabolites content besides the total antioxidant activity was estimated highest in Skeletonema sp. Having strong inhibition zones of 18–20 mm against all the five strains of bacteria also highlights the highest antibacterial prospect in Skeletonema sp. This work manifests the plasticity of diatoms and may provide useful insights for further species-specific selection for large-scale production of eicosapentaenoic acid, docosahexaenoic acid, fucoxanthin and other metabolites with potential health benefits.

Metal–organic frameworks as efficient materials for drug delivery: Synthesis, characterization, antioxidant, anticancer, antibacterial and molecular docking investigation

Metal–organic framework (MOF) nano particles are a class of promising porous nano materials for biomedical applications. Owing to its high loading potential and pH‐sensitive degradation, most promising of the MOFs is the zeolitic imidazolate crystal framework (ZIF‐8), a progressive useful material for small molecule distribution. Doxorubicin (DOX), designated as a classical drug, was jobwise entrapped in ZIF‐8 nano particles. ZIF‐8 nano particles, as a novel carrier, were used to monitor the release of the anticancer drug DOX and prevent it from dissipating before reaching its goal. ZIF‐8 nano particles with encapsulated DOX (DOX@ZIF‐8) can be synthesized in a single pot by incorporation of DOX into the reaction mixture. MOFs and the designed drug delivery (DOX@ZIF‐8) system were characterized by Fourier transfer infrared, scanning electron microscopy, N2sorption isotherm and X‐ray diffraction. The impact of MOFs and the engineered drug delivery system on the viability of human breast and liver cancer cell lines was evaluated. The loaded drug was released at pH 5 faster than at pH 7.4. The nano particles of ZIF‐8 showed low cytotoxicity, while DOX@ZIF‐8 showed high cytotoxicity to HepG‐2 and MCF‐7 cells compared with free DOX at the equivalent concentration of DOX of >12.5 μg/ml. These findings indicate that DOX@ZIF‐8 nano particles are a promising method for the delivery of cancer cells to drugs. Furthermore, ZIF‐8, DOX and encapsulated DOX@ZIF‐8 compounds were screened for their potential antibacterial activities against pathogenic bacteria compared with standard antibiotics by the agar well diffusion technique. The results demonstrate that the DOX@ZIF‐8 exhibits a strong inhibition zone against Gram‐negative strains (Escherichia coli) in comparison with the reference drug gentamycin. The docking active site interactions were evaluated to predict the binding between DOX with the receptor of breast cancer 3hb5‐oxidoreductase and liver cancer 2h80‐lipid binding protein for anticancer activity.

Effect of Milk Thistle (Silybum marianum (L.) Gaertn) Seed Extract on Bacterial Activities and Growth of Human Liver Cancer Cells

Silymarin is a polyphenolic flavonoid mixture isolated from milk thistle which is believed to be responsible for the plant’s hepatoprotective action. Silymarin is hepatoprotective flavonoid drug available as bio marker in Silybun marianum (common name milk thistle). Silymarin is being used in treatment of various liver disease of different etiology because of its hepatoprotective action. Phytochemicals are playing a vital role for the treatment of different types of diseases and still used in both traditional and modern medication system. The phytochemical analysis of milk thistle seeds extract indicated that the plant is rich in secondary compounds. The results revealed that milk thistle seeds contain high amount of total phenolic, flavonoid and antioxidant compounds. The antibacterial activities of the ethanol seeds extract of milk thistle was tested against Gram-positive bacteria (Staphylococcus aures) and Gram-negative bacteria (Salmonella entirica). Antibacterial effects of crude extract were performed using modified Kirby-Bauer disc diffusion technique to determine the zone of inhibition. The results demonstrated that ethanol seeds extract of milk thistle is shown strong inhibition zone against Staphylococcus aures and Salmonella entirica compared to the control. Also, milk thistle seeds extract showed highly anticancer activity. This medicinal plant could be developed into affordable and safe standardized herbal products and may serve as a source of new molecules for broad-spectrum anticancer, antimicrobial agents.

EFFICACY OF EUPHORBIA HETEROPHYLLA LATEX AGAINST PATHOGENIC BACTERIA AND FUNGI

Objective: The objective of the study was to determine the antimicrobial activities of Euphorbia heterophylla latex.
 Methods: The antibacterial and antifungal activities of acetone, chloroform, and diethyl ether extracts were assayed by disk diffusion method.
 Results: The study of plant E. heterophylla latex revealed the presence of medically active metabolites. Bacterial strains such as Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Pseudomonas aeruginosa exhibited a strong zone of inhibition. Acetone extract exerts a potent zone of inhibition against P. aeruginosa compared to tetracycline. Fungi, Aspergillus niger, Fusarium oxysporum, and Penicillium sp., were used for the antifungal activity. It was observed that the highest zone of inhibition was noticed against A. niger in all the extracts. Interestingly, F. oxysporum and Penicillium sp. showed no zone of inhibition and were resistant to standard drug, fluconazole which was used as a control.
 Conclusion: E. heterophylla latex extract was found to be more potent than the standard drugs which were used against both the bacterial and fungal strains.

Antibacterial activities of black soldier flies (Hermetia illucens. l) extract towards the growth of Salmonella typhimurium, E.coli and Pseudomonas aeruginosa

This research was conducted to determine the antibacterial activity of BSF extract in vitro on the growth of Salmonella typhimurium, E. coli and Pseudomonas aureginosa. The experiment was carried out according to the Completely Randomized Design (CRD) consisting of six treatments and three replications for each treatment. The treatments were different concentration levels of BSF extract, i.e. 75, 125, 175, 225, 275 and 325 mg.ml−1. Chloramphenicol with concentration of 30 µg. discs paper−1 was used as a positive control and dimethyl sulfoxide (DMSO) as a negative control. BSF extract was made using maceration extraction method. The results of this study indicated that the antibacterial activity of BSF extract increased (P<0.05) in line with the increase level of BSF extract concentration. The average diameter of the inhibition zone for Salmonella typhimurium, E. coli and Pseudomonas aureginosa was 11.77 ± 0.03 mm, 11.15 ± 0.05 mm, and 11.15 ± 0.23 mm respectively, which was categorized as strong inhibition zone. In conclusion, the concentration of BSF extract of 325 mg.ml−1 is an effective concentration to inhibit the growth of the bacteria Salmonella typhimurium, E. coli and Pseudomonas aureginosa.

Antibacterial activity of basil oil (Ocimum basilicum L) and basil oil nanoemulsion

Basil oil contains bioactive compounds with antibacterial activity. One way to increase the antibacterial activity of basil oil is to use a nanoemulsion design. This study aims to obtain a nanoemulsion of basil oil with the best antibacterial activity against Escherichia coli and Staphylococcus aureus compared to basil oil. Process isolation of basil oils uses the method of steam distillation. The nanoemulsion process uses the ultrasound method. The essential oils and nanoemulsions produced were analyzed by GC-MS. The five components of basil oils with the largest percent area are sabinene (60,01%), myrcene (17,76%), trans-caryophyllene (4,08%), linalool (2,58%), and alpha-pellandrene (2,35%). Whereas in nanoemulsion are sabinene (44,68%), myrcene (17,86%), trans-caryophyllene (8,15%), terpineol-4 (6,65%), and 1.6-octadient-3-ol 3,7-dimethyl (4,89%). The basil oil has a droplet size of 54960 nm while the nanoemulsion of basil oil has a droplet size of 243.4 nm. The concentration of basil oil influences the antibacterial activity. The essential oil of basil, at a concentration of 10% to 25%, has a zone of low to medium protein inhibition at E. coli and is strongly directed towards S. aureus. The nanoemulsion of essential oils at a concentration of 5% to 25% has a moderate to strong inhibition zone in E. coli and a moderate to a very strong group in S. aureus.

Screening of Antibacterial Activity of Goniothalamus calvicarpa Extracts against Xanthomonas axonopodis pv. citri in vitro

Citrus canker caused by Xanthomonas axonopodis pv. citri (Xac) is a serious disease in many parts of the world and is difficult to control. The use of copper-based pesticides is becoming a concern due to the accumulation of heavy metals in orchard soils and more benign treatment methods are needed. Eighteen Thai native plant extracts were screened for antibacterial activity against a Thai isolate of Xac and it was found that ethanolic extracts of Goniothalamus calvicarpa leaves showed the strongest antibacterial activity against Xac in vitro. The G. calvicarpa extracts were then sequentially dissolved with hexane, ethyl acetate and methanol and retested. A 70% aqueous ethanol extract and a methanol soluble extract produced strong inhibition zones against Xac. Although thin layer chromatographic profiles revealed the likely presence of flavonoids in the biologically active extracts of G. calvicarpa, the active compounds have yet to be identified. Work is proceeding to determine whether specific extracts of G. calvicarpa have biological activity against citrus canker in the field.

Antibacterial Activity Test of Ethanol Extract Pineapple (Ananas comosus (L.) Merr.) Peel against Growth of Propionibacterium acnes

Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.

Morphological Structure, Optical and Microbial Findings of Ferrites.

In this article, we report the interesting results on the effect of high energy ball milling (under 350 rpm for 0, 1, 3, 5, and 10 h) on the salt (NaCl:KCl) flux synthesized SrFe12O19 powder. The different processed powder was analyzed by powder X-ray diffraction (XRD), thermogravimetric analysis (TG/DTA), field emission scanning electron microscope (FESEM), Fourier-transform infrared (FT-IR) and near infra-red (NIR) reflectance spectroscopic techniques. From the present research findings, we note that the SrFe12O19 phase is susceptible to the high-energy ball milling process. The unmilled SrFe12O19 powder crystallized in hexagonal structure with the platelet-shaped SrFe12O19 grains exhibits high near-infrared (NIR) reflectance. The average crystalline size (XRD) of the unmilled (84.9 nm) SrFe12O19 powder is reduced to 22.2 nm after 1 h of ball milling. Ball milling of good quality SrFe12O19 powder for >1 h, mixed nanocrystalline phases of SrFe12O19 and α-Fe₂O₃ materials are formed. By increasing the ball milling time, the thermal features also vary accordingly. The platelet-shaped SrFe12O19 grains are strongly affected by ball milling of the salt flux synthesized SrFe12O19 powder for 1, 3, 5 and 10 h. Ball milling of SrFe12O19 powder has a strong effect on the NIR reflectance and color of the resultant ball-milled powders. The 1 h as-milled powder shows relatively strong inhibition zone as compared to other powder samples (0, 3, 5, 10 h) against Pseudomonas aeruginosa bacterium.

CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF Codiaeum variegatum LEAVES

Natural substances of botanical origin have been important in African traditional medical practice. They have been used for various illnesses such as infections. Infectious diseases caused by pathogenic bacteria affect many communities and the treatment was made difficult partly because of antibiotic resistant strains. Phytochemicals isolated from medicinal plants are known to be effective in treating bacterial infections. The antibacterial activities of the ethanol and water leaf extracts of Codiaeum variegatumwere tested. Antibacterial effects of crude extracts were performed using modified Kirby-Bauer disc diffusion technique to determine the zone of inhibition. The extracts were tested for the antibacterial activities against Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Serratia marcescens). The results demonstrated that both of ethanol and water leaf crude extracts of Codiaeum variegatumhave shown strong zone of inhibition against Serratia marcescens (20 mm) and Bacillus subtilis(12 mm) compared to control. This medicinal plant could be developed into affordable and safe standardized herbal products and may serve as a source of new molecules for broad-spectrum antimicrobial agents.

ANTIBACTERIAL ACTIVITY OF WATER AND ETHANOLIC EXTRACTS OF Bauhinia variegata LEAVES

Phytochemicals isolated from medicinal plants are known to be effective in treating bacterial infections. The antibacterial activities of the ethanol and water leaf extracts of Bauhinia variegata were tested. Antibacterial effects of crude extracts were performed using modified Kirby-Bauer disc diffusion technique to determine the zone of inhibition. The extracts were tested for the antibacterial activities against Gram-positive bacteria (Sarcina ventriculi) and Gram-negative bacteria (Serratia marcescens). The results demonstrated that both ethanol and water leaf extracts of Bauhinia variegata have shown strong inhibition zone against Serratia marcescens and Sarcina ventriculi compared to control). This medicinal plant could be developed into affordable and safe standardized herbal products and may serve as a source of new molecules for broad-spectrum antimicrobial agents. Our results revealed that Bauhinia variegata leaves contains total phenolic contents (453 mgGAE/ g), flavonoid 166 mg QE/g and total alkaloid 1.674 g/100g. Also Bauhinia variegata leaf extracts showed highly antioxidant activity.

Determination of Antimicrobial Activity and Phytochemical Screening of Selected Medicinal Plants in Tigray region of Northern Ethiopia

Present paper evaluates the antimicrobial activities and preliminary phytochemical screening of the extracts of Beciumgrandiflorum, Meriandrabengalensis, Tamarindusindica, Balanitesaegyptiaca and Otostegiaintegrifolia . Comparative study was made for the selected traditional medicinal plants. The results of phytochemical test indicate that the bioactive chemical components show presence of alkaloids, flavonoids, phenols, carbohydrates, saponins and terpenoids. The antimicrobial activities of aqueous and ethanol extracts of leaf and stem were determined on the selected bacteria strains. The results indicate that the aqueous and ethanol leaf extracts of Meriandrabengalensis show better antimicrobial activity against Staphylococcus aureus and Staphylococcus pneumonia with inhibition zone diameters of 15.97 ± 0.09 and 19.03 ± 0.15 mm respectively, significant at (P<0.05) compared to other extracts. From the phytochemical components tested, carbohydrate was present in Becium grandiflorum extract. The lowest inhibition zone (4.5 ± 0.29 mm) was achieved for the extract of Balanitesaegyptiaca. The phytochemical component saponinis significantly present in Meriandrabengalensis in the aqueous leaf extract. In the comparative study made for the extraction solvents aqueous and ethanol, the aqueous extraction had strong inhibition zone. Therefore, a study need to be done on the isolation, identification, and quantitative determination of antimicrobial components present in the Meriandrabengalensis for its application in both animal and human pharmaceutical industries. Keywords : Antimicrobial activities; Phytochemical screening; Extracts; Inhibition zone; Medicinal plants; Tigray; Ethiopia.

Detection of antifungal compounds in Arabidopsis thaliana and brassica oleracea by thin layer chromatography .

Thin Layer Chromatography (TLC) was applied to detect antifungal compounds both in Arabidopsis thaliana and Brassica oleracea after elicited by various biotic and abiotic elicitors.From TLC bioassays the only strong zone of inhibition detected after challenged by Pseudomonas syringae pv.maculicola was from Arabidopsis tissue and later confirmed by spectrophotometry as camalexin but no corresponding phytoalexin was found in broccoli leaf.

Simultaneous photoinduced electron transfer and photoinduced CuAAC processes for antibacterial thermosets

A combination of simultaneous photoinduced electron transfer and photoinduced CuAAC processes enables the in-situ preparation of antibacterial thermosets containing silver nanoparticles (AgNPs) in one-pot. Upon photolysis of photoinitator, the generated radicals not only reduce Cu(II) into Cu(I) activator to catalyst the CuAAC click reaction, but also simultaneously generate AgNPs from AgNO3 through electron transfer reaction. Due to their reduction potentials difference, the polymer matrix is formed before the formation of AgNPs, assisting to eliminate the agglomeration of them. The thermoset structures are confirmed by FT-IR and solubility tests, whereas the presence of AgNPs is proven by transmission electron microscopy with energy dispersive X-ray system analyzer. The samples containing 5 and 10% AgNPs exhibited strong inhibition zones, where all kinds of bacteria (gram-positive (Staphylococcus Aureus) and gram-negative (Escherichia Coli)) were killed in the surrounding of the film samples.

Diversity and Antifungal Activity of Actinomycetes Symbiont Hard Coral Mucus of Genera Goniopora and Porites

Screening new bioactive compounds from marine actinomycete organisms associated with corals (Goniopora and Porites) can be an alternative method to discover the natural antifungal compounds. This study aims to determine the density and diversity of actinomycete symbionts based on repetitive sequence-based-polymerase chain reactions ( rep- PCR) and to discern the ability of antifungal activity of isolates symbiotic with hard coral mucus by using a pour plate method. A total of 143 isolates were obtained from the hard coral mucus of genera Goniopora and Porites. H igh genetic diversity was observed among the isolates . Ten isolates with different morphological characteristics were selected to extract its secondary metabolites and then followed by an antifungal test. The isolate with the code of SCAS324 was the one with the antifungal activity, marked by the formation of a very strong inhibition zone of 54.7±0.4 mm toward Aspergillus flavus and 49.2±2.7 mm toward Candida albicans . Antifungal screening showed that the antifungal activity of the isolate SCAS324 was three times as effective as the commercial antifungal.