Event Abstract Back to Event Evaluation of Total Phenolic, Flavonoids, and Antioxidant Activities of Crude and Fractionated Extracts of Selected Local Medicinal Plants Phaik Har Yong1*, Wai Hin Chan1, Mei Rong Chong1, Kuan Zheng Chu1 and Xiao Jiun Tan1 1 Mahsa University, Malaysia Background Recent studies have suggested that the development of metabolic syndrome complications could be reduced by the intake of natural antioxidants from plants. Medicinal plants have been used as traditional medicine for the treatment of various diseases related to oxidative stress by local populations in Malaysia. Hence, this study hypothesizes that the selected medicinal plant extracts would be able to demonstrate significant levels of phenolic and flavonoid compounds which possess anti-oxidant activities. The objective of this study was to evaluate the antioxidant potential of Centella asiatica, Phyllanthus urinaria, Folium mori, Rhinacanthus nasutus, Alternanthera sessilis and Strobilanthes crispus. Methods Selected local plants were processed and macerated with absolute methanol. The solvent extracts were rotary evaporated, subjected to fractionation with different solvents in the following order: water, hexane, chloroform, ethyl acetate and n-butanol. The antioxidant activities of extract and fractions were evaluated through Ferric Reducing Antioxidant Power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) free radicals scavenging assays. Folin-Ciocalteu and aluminium chloride methods were used to quantify the phenolic, flavonoid contents of crude extracts and fractions. Results P. urinaria demonstrated considerable levels of phenolic, flavonoids and antioxidant activities across all extract fractions. In contrast, only a few selected fractions of other plant samples showed high levels of activities. These were particularly of ethyl acetate, n-butanol and chloroform fractions which possessed high levels of phenolic, flavonoids and antioxidant activities. Ethyl acetate fraction of C. asiatica displayed the highestphenolic, flavonoid contents and antioxidant activities when compared to the other plant samples. In general, the phenolic (r = 0.875, p<0.001) and flavonoid contents (r = 0.527, p<0.001) were positively correlated with total antioxidant activities. Conclusion The study showed that the selected medicinal plants, particularly P. urinaria possess high antioxidant potential and could be a potential natural source of antioxidant. Keywords: Antioxidants, medicinal plants, Phenolic, Flavonoids, Fractionation Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Miscellaneous Citation: Yong P, Chan W, Chong M, Chu K and Tan X (2019). Evaluation of Total Phenolic, Flavonoids, and Antioxidant Activities of Crude and Fractionated Extracts of Selected Local Medicinal Plants. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00090 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Sep 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Phaik Har Yong, Mahsa University, Kuala Lumpur, Malaysia, yphaikhar@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Phaik Har Yong Wai Hin Chan Mei Rong Chong Kuan Zheng Chu Xiao Jiun Tan Google Phaik Har Yong Wai Hin Chan Mei Rong Chong Kuan Zheng Chu Xiao Jiun Tan Google Scholar Phaik Har Yong Wai Hin Chan Mei Rong Chong Kuan Zheng Chu Xiao Jiun Tan PubMed Phaik Har Yong Wai Hin Chan Mei Rong Chong Kuan Zheng Chu Xiao Jiun Tan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.