Poly- Glutamic acid is an edible, bio-degradable natural bio-polymer possessing well- established heavy metals adsorbent and removal properties. In the present work, a sensitive and selective method for the determination of Pb(II) ions using poly glutamic acid modified graphene electrodes has been developed. The PGA/rGO electrode was employed for the pre-concentration followed by the subsequent anodic stripping analysis of metal ions. The developed sensor showed good sensing characteristic for lead ions and eliminated interferences with other metal ions under the optimized conditions of pH and electrolyte. The modified electrode was further characterised by SEM, IR and AFM spectroscopy. Electro-analytical characterisations were carried out using cyclic voltammetry and square wave voltammetry techniques. The stripping response for the Pb(II) was found to be linear over the concentration range of 0.2–115 μg/L. The detection limit is 0.06 μg/L. The developed sensor was applied for the determination of Pb(II) in well water samples to ascertain the applicability of the proposed method towards real samples.