Stripping Reagent Research Articles

PVDF-HFP based polymer inclusion membranes containing Cyphos® IL 101 and Aliquat® 336 for the removal of Cr(VI) from sulfate solutions

Poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) was used for the preparation of polymer inclusion membranes (PIMs) containing trihexyltetradecylphosphonium chloride (Cyphos® IL 101) or trioctylmethylammonium chloride (Aliquat® 336) for the removal of Cr(VI) from sulfate solutions. PIMs containing 50 wt% of Cyphos® IL 101 or Aliquat® 336, with corresponding thicknesses of 54 ± 7 or 48 ± 9 μm, were transparent and mechanically stable. Scanning electron microscopy, atomic force microscopy, thermogravimetric analysis, stress–strain analysis, and contact angle measurements were used for characterizing PIM surface morphology, thermal and mechanical properties. The extraction experiments were performed by immersing a circular PIM segment (3.5 cm in diameter) in a 50 mL sulfate solution containing 2.0 × 10−4 mol L−1 of Cr(VI). The optimum conditions with respect to pH and sulfate concentration were 1.2 and 0.045 mol L−1, respectively. The results indicated a higher extraction rate of Cr(VI) by the PIM containing Cyphos® IL 101 in comparison with that achieved by the Aliquat® 336 based PIM. Analysis of the extraction data confirmed that the main extracted species into both PIMs was HCrO4−. A variety of stripping reagents were tested and best results were obtained with 1.0 mol L−1 NaNO3 which allowed efficient back-extraction of Cr(VI) from the loaded PIMs containing Cyphos® IL 101 or Aliquat® 336. The extraction capacity of the Cyphos® IL 101 and Aliquat® 336 based PIM was 71 mg and 70 mg of Cr per 1 g of membrane and they could be reused for 16 and 6 consecutive extraction/back-extraction cycles, respectively. It was found that the mass loss of the Cyphos® IL 101 PIMs in these experiments was significantly lower than that observed for the Aliquat® 336 based PIMs. These results illustrated the excellent stability of the Cyphos® IL 101 based PIM. Both Cyphos® IL 101 and Aliquat® 336 based PIMs did not experience interference in the extraction of Cr(VI) by other common cations. The Cyphos® IL 101 based PIM extracted selectively Cr(VI) in the presence of up to 500 fold excess of nitrate and chloride anions, while this selectivity was limited to 10 fold for the Aliquat® 336 based PIM. The potential of both PIMs for practical applications was demonstrated with the removal of Cr(VI) from well water and industrial wastewater.

Removal of picloram herbicide from an aqueous environment using polymer inclusion membranes

Abstract The picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) herbicide is widely applied to control the growth of woody plants and it has been frequently detected in aqueous environments due to its poor adsorption by soils and high leaching potential. Consequently, the removal of picloram from contaminated environmental waters is of major concern due to its adverse impacts on aquatic organisms, soils and animals. The development of a method for the extraction and transport of picloram from aqueous solutions using a polymer inclusion membrane (PIM) composed of cellulose triacetate, trioctylmethylammonium chloride (Aliquat 336) and 2–nitrophenyl octyl ether (NPOE) is reported. The experimental method was optimized for the PIM composition, and the type and concentration of stripping reagent. The optimized method demonstrated good performance indicators of flux and transport efficiency for picloram during non-competitive and competitive transport experiments. The PIM was also successfully applied in a passive sampling device to recover picloram at the maximum permissable concentration of 500 μg/L from a complex matrix of natural water. Findings from this study demonstrate that PIMs can serve as a potential alternative method for the removal and recovery of picloram and related herbicides from contaminated aqueous solutions.

Selective transportation of molybdenum from model and ore through poly inclusion membrane

The extraction of molybdenum from the aqueous solution through poly inclusion membrane (PIM) containing tri-caprylylmethylammonium chloride (Aliquat-336) as a carrier has been investigated. A solution of molybdenum in phosphoric acid was used as a feed side while sodium hydroxide was used as stripping reagent. The results indicate that maximum flux value was obtained at 0.16 M Aliquat-336. Increase in H3PO4 concentration from 0.05 to 1.5 M results into an increase in molybdenum ions. The maximum flux of 3.00×10−6 mol/m2s through PIM was found at 1.5 M H3PO4. The optimized conditions were applied for removal of Mo(VI) from ore and more than 97 % Mo(VI) was extracted.
 
 Bull. Chem. Soc. Ethiop. 2020, 34(1), 93-104.
 DOI: https://dx.doi.org/10.4314/bcse.v34i1.9

Chondroitin sulfate-functionalized 3D hierarchical flower-type mesoporous silica with a superior capacity for selective isolation of low density lipoprotein

Novel flower-type and three-dimensional porous nanoparticles are prepared for the isolation of low density lipoprotein (LDL). The amino-terminated dendritic mesoporous silica nanoparticles (A-DMSNs) show highly accessible central-radial pore (0.655 cm3 g−1) and surface area (362.263 m2 g−1), which play an important role in the superior adsorption capacity of 816.7 μg mg−1. The A-DMSNs is anchored with chondroitin sulfate (CS), shortly termed as ADC, for ensuring the selectivity of the adsorption. The adsorbed LDL is thereafter readily recovered at pH 9.0 by using 4 mmol L−1 Britton-Robinson buffer as stripping reagent, providing a recovery of 79.1%. ADC nanoparticles are further applied as sorbent for the selective isolation of LDL from simulated serum of hypercholesterolemia patient. High-purity LDL is achieved as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assays.

Separation and recovery of phenols from an aqueous solution by a green membrane system

Abstract Oil-refinery alkali residue wastewater (ORARW) contains large amounts of phenol and its derivatives, which are highly toxic and valuable but difficult to biodegrade. In this study, using vegetable oil as the liquid membrane phase, a green polypropylene-hollow-fiber supported liquid membrane (SLM) system was constructed to simultaneously separate and recover phenol, m-cresol and o-cresol from aqueous solutions. After screening several types of vegetable oil, linseed oil was selected as the liquid membrane phase. Based on a single SLM module and using a NaOH solution as the stripping reagent with feed phase and stripping phase hydraulic retention times (HRTs) of 7.8 and 6.9 min, respectively, the phenol separation and recovery rates were 92.9% and 88.8%, respectively. The separation rates for m-cresol and o-cresol were both greater than 96%, their recovery rates were approximately 92%, and the membrane separation flux of every phenolic compound exceeded 0.40 g/m2·h. When two SLM modules were operated in series at room temperature for 3 h, the separation efficiency for phenols increased by approximately 9% compared with that obtained with a single SLM module. After applying two SLM modules in series to treat ORARW with an initial concentration of phenols of 4800–5200 mg/L, the simultaneous separation and recovery rates of phenols were near 95% and 92%, respectively. This work concluded that vegetable oil can be used as a nontoxic and renewable green liquid membrane phase and that two SLM modules in series efficiently separated and recovered phenols from aqueous solutions.

Rapid and multi-cycle smFISH enabled by microfluidic ion concentration polarization for in-situ profiling of tissue-specific gene expression in whole C. elegans.

Understanding gene regulation networks in multicellular organisms is crucial to decipher many complex physiological processes ranging from development to aging. One technique to characterize gene expression with tissue-specificity in whole organisms is single-molecule fluorescence in situ hybridization (smFISH). However, this protocol requires lengthy incubation times, and it is challenging to achieve multiplexed smFISH in a whole organism. Multiplexing techniques can yield transcriptome-level information, but they require sequential probing of different genes. The inefficient macromolecule exchange through diffusion-dominant transport across dense tissues is the major bottleneck. In this work, we address this challenge by developing a microfluidic/electrokinetic hybrid platform to enable multicycle smFISH in an intact model organism, Caenorhabditis elegans. We integrate an ion concentration polarization based ion pump with a microfluidic array to rapidly deliver and remove gene-specific probes and stripping reagents on demand in individual animals. Using our platform, we can achieve rapid smFISH, an order of magnitude faster than traditional smFISH protocols. We also demonstrate the capability to perform multicycle smFISH on the same individual samples, which is impossible to do off-chip. Our method hence provides a powerful tool to study individual-specific, spatially resolvable, and large-scale gene expression in whole organisms.

PEGylation of metal-organic framework for selective isolation of glycoprotein immunoglobulin G

Metal-organic frameworks (MOFs) have been gaining extensive popularity in the field of sample pretreatment due to their designable structures and easy-modifiable surfaces. Herein, zirconium-based MOFs UiO-66-NH2 is first coated with graphene oxide (GO) layer to enhance the stability, and then grafted with hydrophilic polyethylene glycol (PEG) via amidation reaction to give a product of hydrophilic MOF-based nanomaterial UiO@GO@PEG. The obtained nanoparticles exhibit typical hydrophilic interaction liquid chromatography (HILIC) behavior towards glycoproteins, making itself powerful adsorbent for the extraction of glycoproteins from complex matrices. Under pH 9.0, an adsorption capacity of 139.6 mg g−1 for glycoprotein immunoglobulin G (IgG) is achieved, and the retained IgG could be recovered by 0.5% (m/v) ammonium hydroxide (NH4OH) as stripping reagent with a recovery rate of 82.6%. Moreover, the UiO@GO@PEG shows excellent reusability in IgG isolation. The practicability of UiO@GO@PEG is verified by the selective isolation of IgG from human serum, demonstrating the purity of isolated IgG with SDS-PAGE assay.

Preparation of Tungstotellurate(VI)-coated Magnetic Nanoparticles for Separation and Purification of Ovalbumin in Egg White

Abstract Tungstotellurate(VI)-coated magnetic nanoparticles (MNP) were prepared by immobilization of tungstotellurate(VI) salt onto the surface of branched polyethyleneimine (PEI)-modified magnetic nanoparticles via electrostatic interaction. The structure and composition of TeW@MNP were confirmed by characterization with FT-IR, EDS and SEM. The obtained TeW@MNP composite had proven to be a promising adsorbent for the adsorption of ovalbumin in egg whites. Approximately 0.5 mg of TeW@MNP composite gave rise to a maximum adsorption efficiency of 91.6% for 100 μg mL–1 ovalbumin in 200 mL of sample solution at pH 4.0 (BR buffer). The retained ovalbumin was readily recovered by using 0.1% SDS as a stripping reagent, providing a recovery of 98.1%. The adsorption behavior of ovalbumin fitted Langmuir model, corresponding to a theoretical adsorption capacity of 373 mg g–1. The TeW@MNP composite was practically applied to the selective isolation of ovalbumin from egg whites, and SDS-PAGE assay results clearly indicated that the ovalbumin with high purity was obtained. The above experimental results illustrated the great application potentials of polyoxometalates for protein isolation and purification and extended the application scope of polyoxometalates in life science field.

Efficient Removal of Fluoride by Complexation Extraction: Mechanism and Thermodynamics

A complexation extraction system was designed to develop a new process for the efficient removal of fluoride from solutions, such as zinc or copper electrolytes or wastewater derived from flue gas. The effects of the boron fluoride molar ratio, organic phase composition, initial pH, temperature, and phase volume ratio on the extraction efficiency were investigated. The extraction efficiency was found to increase with the increase in the boron fluoride molar ratio, Alamine336 concentration, and phase volume ratio, whereas it decreased with the increase in temperature. For the simulated electrolyte or wastewater derived from flue gas, the majority of metallic ions were insensitive to the extraction, with the exception of Al3+ and Fe3+. Fluoride decreased from 5 g/L to 0.05 g/L after two-stage cross-flow extraction alone; with an extraction efficiency of 99%. Both the stripping and cycling properties were excellent when sodium hydroxide was employed as the stripping reagent. Furthermore, the loading capacity was 43.4 g/L, and increased by four times, when boric acid was added. This novel process implies a wide range of potential applications, such as the removal of unwanted fluoride ions from various high-fluoride polluted solutions and the simplification of brine phase diagram.

Selective separation of bovine hemoglobin using magnetic mesoporous rare-earth silicate microspheres

Selective separation of heme proteins and peptides from complicated biological samples before comprehensive identification and characterization of intact biomolecules which are low stoichiometry is indispensable for ongoing proteomics. Here, three magnetic mesoporous rare-earth silicate (rare-earth = Er, Tm, Yb) microspheres prepared by facile solvothermal method were used as novel adsorbents for the selective isolation of bovine hemoglobin (BHb), and magnetic mesoporous ytterbium silicate showed apparent adsorption efficiency in isolating BHb with a adsorption capacity of 304.4 mg/g after the comparison. The retained BHb could be eluted by using Na2CO3 as stripping reagent. Meanwhile, Circular dichroism spectra illustrated that the microspheres posed no effect on the secondary structure of BHb. The resultant magnetic particles were characterized by transmission electron microscope, scanning electron microscope, x-ray powder diffraction, vibrating sample magnetometer, Fourier transform infrared spectroscopy, N2 adsorption−desorption isotherm and zeta potential. The as-prepared magnetic microspheres showed high specificity for the separation of BHb from bovine plasma as corroborated by SDS-PAGE and MALDI-TOF MS analysis, which would be expected to possess potential application in isolation of other heme proteins in complex biological samples.

Non-Dispersive Extraction of Ge(IV) from Aqueous Solutions by Cyanex 923: Transport and Modeling Studies

Transport process of germanium from an aqueous solution containing oxalic acid and 100 mg/L Ge was studied. Cyanex 923 immobilized in a polytetrafluoroethylene membrane was employed as a carrier in a flat-sheet supported liquid membrane (FSSLM) system. The speciation of the germanium ion in the oxalic acid medium and related diagrams were applied to study the transport of germanium. The effective parameters such as oxalic acid, carrier concentration, and strip reagent composition were evaluated in this study. Based on the experimental data, the oxalic acid and carrier concentrations of 0.075 mol/L and 20% v/v were the condition in which the efficient germanium transport was achieved, respectively. The concentration range of 0.04–0.1 mol/L was selected for sodium hydroxide (NaOH) as a strip reagent providing the best efficiency to transport germanium through the supported liquid membrane (SLM) system. Furthermore, the permeation model was obtained to calculate the mass transfer resistance of the membrane (Δm) and feed (Δf) phases. According to the results, the values of 1 and 1345 s/cm were found for Δm and Δf, respectively.

PGM ore processing: LIX reagents for palladium extraction & platinum stripping from Alamine 336 using NaOH-NaCl

Solvent extraction can be operated as a batch process or in counter-current mode. The preference for an extractant changes depending on the mode of operation. The extraction kinetics and isotherms are studied for the extraction of palladium using various LIX reagents. It was found that LIX 64N is preferable for counter current extraction, while LIX 984 is suitable for batch extraction of palladium. Further, platinum was stripped from Alamine 336 with a solution of NaOH-NaCl. This reagent is capable of stripping 0.75 g/l of platinum from Alamine 336 in a single contact at A/O of 1. Unlike the thio-reagents studied earlier, this novel stripping reagent is environment friendly. The two studies have been applied for recovering palladium and platinum from ore samples. Palladium and platinum were separated with a purity of >98%.

Separation of Pb(II) Ion with Tetraacetic Acid Derivative of Calix[4]arene by Using Droplet-based Microreactor System

In this study, the microreactor system was investigated and compared with the batch-wise system as rapid and effective extractive Pb(II) separation over Fe(III), Cu(II) and Zn(II) with tetraacetic acid calix[4]arene. By using a microreactor system, the Pb(II) extraction percentages reached the maximum of 73, 89 and 100% in 8 sec residence time at equilibrium pH of 2.00, 2.25 and 2.50, respectively. The stripping percentage was 92% at 8 sec residence time by using a microreactor system with 2.0 M HNO3 as a stripping reagent. Complete separation of Pb(II) over Fe(III), Cu(II) and Zn(II) ions with the tetraacetic acid calix[4]arene in a competitive metal system was achieved at pH 2.00. However, the batch system required 24 h to reach the equilibrium for both extraction and stripping processes. The results suggested that the microreactor system enhanced the Pb(II) extraction and stripping rate up to 104 times faster than the batch-wise system.

Micro-Calorimetric Study on the Thermal Stability of N, N-Dimethylhydroxylamine in the APOR Process

The thermal stability of N, N-dimethylhydroxylamine (DMHAN) in the HNO3 solution was studied using microcalorimeter. The influence of concentration of HNO3, DMHAN, methylhydrazine (MH), atmosphere (air and nitrogen), and metals was investigated. The kinetic parameters and self-accelerating decomposition temperature (SADT) of the feed in process (stripping reagents 1BX, scouring agent 2DS, stripping reagents 2BX, and waste aqueous phase 2DW) were calculated by Advanced Kinetics and Technology Solutions (akts) thermokinetics software. The molar enthalpy of the reaction of NaNO2 with DMHAN and MH was also determined. The results show that the initial reaction temperature (T0) of DMHAN/HNO3 (HNO3: 1.5–3.0 mol/L, DMHAN: 0.05–0.8 mol/L) is increased as the acidity is reduced or the concentration of DMHAN is increased. Holding reductant MH made the induction period of the autocatalytic reaction longer. The air, nitrogen atmosphere, Fe, and the fission products (Zr, Ru) do not affect the decomposition of DMHAN, but the stainless steel made the T0 of DMHAN/HNO3 become lower. The SADT of 1BX/2DS, 2BX, and 2DW is 56 °C, 52 °C and 47 °C, respectively. The molar enthalpies of formation of the reaction of NaNO2 with DMHAN and MH are −411.3 kJ/mol, −246.0 kJ/mol, respectively.

Improvement on the extraction efficiency of low density lipoprotein in an ionic liquid microemulsion

A novel microemulsion is developed at room temperature with 30 µL of sodium alginate sulfate (SAS, 0.02 mol/L), 0.005 g bis (2-ethylhexyl) succinate sulfonate (AOT) and 270 µL of 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF6) ionic liquid as aqueous phase, surfactant and IL phase, respectively. The SAS/AOT/BmimPF6 microemulsion significantly improves the extraction efficiency for low density lipoprotein (LDL). 96% LDL in a 300 µL of PBS is selectively extracted into a same volume of microemulsion, with respect to those of 67%, 76% and 85% by BmimPF6, H2O/AOT/BmimPF6 microemulsion and sodium alginate (SA)/AOT/BmimPF6 microemulsion. LDL in the SAS/AOT/BmimPF6 microemulsion is distributed both in BmimPF6 via hydrophobic interaction and in the “pools” of the microemulsion via electrostatic interaction with AOT and specific interaction between LDL with SAS. 83% of LDL in the microemulsion can be readily back extracted into an aqueous phase with 0.8% (m/v) of sodium dodecyl sulfate (SDS) as stripping reagent. For practical applications, LDL in human serum is selectively extracted with the microemulsion, as demonstrated by enzyme linked immunosorbent assay (ELISA) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Separation and recovery of copper from waste printed circuit boards leach solution using solvent extraction with Acorga M5640 as extractant

ABSTRACTWaste printed circuit boards (WPCBs) have received extensive attention in recent years because of its harmfulness and resource. In this work, two-step leaching process was carried out by using steel pickling waste liquor (SPWL) as the leaching agent. The leaching solution contains a variety of metals, especially iron, which will have an effect on the recovery of copper. Acorga M5640 (M5640) extractant with a kerosene diluent was used to recover copper from WPCBs leach solution, and the separation factor is adopted to analyze the effects of these metal ions. The effect of different parameters such as pH of aqueous phase, phase ratio (O/A), M5640 concentration, contact time as well as the concentration of H2SO4 as stripping reagent were investigated. Over 90.0% copper was extracted with pH 1.1, phase ratio (O/A) 1/1, M5640 concentration 16%, contact time 3 min at room temperature. For the stripping process, the 60 s contact time and 2.5 mol/L H2SO4 concentration are suitable with 90.0% stripping percentage of copper. Copper extraction isotherm accords with Langmuir isotherm equation and the results show that iron is the most influential metal ion for copper extraction, which will reduce the theoretical saturation of the extractant. The extractant M5640 has excellent reuse performance and can be recycled more than 10 times, which demonstrated M5640 has the industrial application value in the extraction of copper from WPCBs leach solution.

A rapid and efficient lithium-ion recovery from seawater with tripropyl-monoacetic acid calix[4]arene derivative employing droplet-based microreactor system

A rapid and efficient lithium-ion recovery from seawater established by employing calix[4]arene derivatives as extraction reagents in a microreactor system. A tripropyl-monoacetic acid derivative of calix[4]arene (1Ac) exhibited a very high extraction ability for lithium-ion within 2.00 s by using a microreactor system. The complete stripping of the lithium ions achieved with 1.0 M HCl as a stripping reagent. In a competitive metal system containing lithium, sodium, potassium, rubidium, cesium, magnesium, calcium, strontium and barium ions; lithium-ion selectively loaded on 1Ac. Further, lithium recovery from seawater was carried out without any pretreatment or pH adjustment in the three-step process. In the first step of the process, 1Ac extracted 100% lithium and 2.56% sodium ions from the seawater. Both lithium and sodium ions stripped with HCl in the second step and then sodium in the stripped solution selectively re-extracted with monopropyl-triacetic acid calix[4]arene derivative (3Ac) thus yielding pure lithium chloride in the aqueous phase. Both 1Ac and 3Ac calix[4]arene derivatives regenerated after the stripping process.

Recycling of colored office paper. Part II: Post-bleaching with formamidine sulfinic acid and hydrogen peroxide

In this study, the optimization of formamidine sulfinic acid (FAS) and hydrogen peroxide (P) bleaching stages of pre-bleached blue, red, yellow, green, and mixed colored office papers was investigated. The FAS was performed as a strong color stripping reagent on blue and green colored samples. However, due to reduced amine compounds FAS was not found to be effective on the red colored samples. In contrast, effective results were obtained with hydrogen peroxide on the red and yellow colored samples. These results showed that the two reagents must be used together in a sequence. Therefore, in this study, FASP and PFAS bleaching sequences were also investigated and the optical properties (absorbance (k/s) spectra, CIE L*a*b* color values, and ISO brightness) of these sequences were compared. The color differences (CIE ΔE) of FASP and PFAS bleaching sequences of the mixed colored samples were calculated as 18.2% and 16.1%, respectively. The FASP bleaching sequence was determined to be more effective than the PFAS sequence for the bleaching of all samples. As a result, this study showed that FAS, a strong reducing agent, and hydrogen peroxide, an effective oxidative decolorizer, can be used together to obtain white writing papers from waste direct dye colored office papers.

A conceptual hybrid process flowsheet for platinum group metals (PGMs) recovery from a chromite-rich Cu-Ni PGM bearing ore in oxidized mineralization through a single-stage leach and adsorption onto ion exchange resin

The availability of oxidized PGM ore deposits, at or near the earth's surface, present lucrative prospects for future PGM production owing to the large resource estimates, the relatively high PGM grades and they can be mined through relatively lower-cost open-pit mining processes. However, these oxidized PGM ores are currently not mined or treated due to insufficient recoveries using established metallurgical techniques. The complex mineralogy and polymodal distribution of PGM in the oxidized ores pose a number of challenges to the metallurgist from a mineral liberation and extraction perspective.A conceptual route to the integrated processing of a Ni-Cu-PGM bearing chromite ore in oxidized mineralization for the recovery of base and precious metals is presented based upon earlier work of the authors and recent research on metal recovery from solution. This paper presents laboratory batch-scale results of simultaneous adsorption of Pd(II) and Pt(IV) onto commercially available weak-base anion exchange resin, diethylaminoethyl (DEAE) cellulose, from hydrochloric acid solutions. Effects of various variables such as equilibration time, thiocyanate and tin chloride concentrations were studied.Without any prior scrubbing step to remove co-extracted impurities, the uptake of Pd(II) and Pt(IV) was successfully accomplished with recoveries approximating 100% within 15 min. The presence of Fe(III) and other non-ferrous metal accompanying ions didn't show any marked tendency to adsorb. Among the adsorption parameters, thiocyanate concentration had the most significant effect on the process. The dual role of Sn(II) i.e. labilizing agent and PGM collector at a time, was simply overridden, overshadowed by the DEAE high selectivity for PGMs. Thiourea proved to be a better stripping reagent for bulk elution of combined Pd(II) and Pt(IV). A conceptual flowsheet is disclosed for hydrometallurgical pilot scale testing, economic evaluation and assessment of potential environmental impacts.

Specific Isolation of Glycoproteins with Mesoporous Zirconia-Polyoxometalate Hybrid.

A novel mesoporous zirconia-polyoxometalate ZrO2 -P8 W48 hybrid was prepared using a surfactant-assisted solvent evaporation technique. The acid-base reaction between the Zr-OH groups of zirconium oxides and P8 W48 was followed by self-assembly with an amphiphilic triblock copolymer as template to obtain a polyoxometalate-based hybrid. The ZrO2 -P8 W48 hybrid was characterized by Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric assay (TGA), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), energy-dispersive X-ray spectroscopy (EDXS), X-ray diffraction (XRD), and nitrogen sorption/desorption. Owing to the multiple hydrogen-bonding interactions between the P8 W48 moiety and the hydroxyl groups of glycoproteins, the ZrO2 -P8 W48 hybrid exhibited highly selective isolation of glycoproteins from complex matrices that included various non-glycoproteins. The retained glycoproteins could be readily recovered using a 0.01mol L-1 cetane trimethyl ammonium bromide (CTAB) solution as stripping reagent, with recovery rates of 92, 100, 100, 100, and 74% for the five target glycoproteins, Ovalbumin (Ova), conalbumin (ConA), immunoglobulin G from human serum (IgG), γ-globulin from bovine milk (γ-Glo), and horseradish peroxidase (HRP), respectively. The ZrO2 -P8 W48 hybrid was successfully applied to the isolation of glycoproteins from egg white and human serum samples, as confirmed by SDS-PAGE and LC-MS/MS assays.